MATERI 7PERKEMBANGAN KONSEPTUS

MK. Ilmu Reproduksi

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SUB POKOK BAHASAN

• Proses pembelahan sel zygote

• Differensiasi sel embrio dan perkembangan embrio awal

• Proses implantasi

• Faktor-faktor yang mempengaruhi perjalanan embrio hingga terjadinya implantasi

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Oviductal Transport

in theMare

Ovulation

Ovary

Oviduct

Uterine Horn

UnfertilizedOva

Blastocyst

PGE Release

Only fertilized ova are transportedinto the the uterine horn of the mare

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4

Ovulasidan Fertilisasi

Early Embryonic Development

All cell divisionsare mitotic Smallercell size with eachcleavage division

SeparateBlastomere’sfor twins

Enters uterus -Even if not fertilizedExcept Mare- Unfertilized remainin oviduct

Fuse twomorulatogether fora chimera

Ampullary- 0-2 One cellIsthmicJunction

1-3 Two cellAmpullary-IsthmicJunction

Isthmus 2-3 Four cell

Isthmus 3-5 Eight cell

Uterus 4-5 Sixteencell

Uterus 5-8 Morula

Location Day Development

Two PolarBodies

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6

Day Development

6-7 Tight MorulaCompact Morula formtight cell contacts so thatBlastocyst formation is possible

7-8 Early Blastocyst

7-9 Blastocyst

Fills with fluid in center -Cells have established polarity

Blastocoel

8-10 Expanded Blastocyst

Trophoblast -Forms Placenta

Inner Cell Mass -Forms Embryo

If split ICM get twins

9-11 Hatching Blastocyst

Contraction and expansion of blastocystas well as enzymes are involved withzona removal

Picture reproduced with permission of Academic Press In: Physiology and Technology of ReproductionPicture courtesy of Dr. J.E. Flechon

Unpolarized Morula Polarized Morula

Blastocyst

Blastocoel

Inner CellMass

TrophectodermBlastocoel

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8

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9

Embrio Hatching

Terjadi Implantasi

(Ternak Bunting)

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10

Embryo sapi

Blastocyst - Nuclei

Inner CellMass

Photomicrograph courtesy of Dr. Daniel Pomp11

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Time comparisons from ovulation during cleavage for different farm species

1 cell 8 Cell Blastocyst Enter Uterus Length ofSpecies (hours) (Days) Gestation

Cattle 24 3 8 3.5 281

Horse 24 3 6 5 337

Sheep 24 2.5 7 3 148

Swine 14-16 2 6 2 114

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Differentiation o f Conceptus Cell Types

1

2

3Three Layers Form Day 14

Expanded Blastocyst

Inner Cell Mass

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