Bioreactor Design Nur Istianah, ST.,MT.,M.Eng

TEKNOLOGI BIOPROSES

Nur Istianah-THP-FTP-UB-2017

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Bioreactor

Project!

An industry need to produce 100

ton per day of bioethanol from

cassava tubers. It is suplaid by

2000 ton cassava tubers per day.

Could you tell us how about the

spesification of bioreactor should

we use?

Nur Istianah-THP-FTP-UB-2017

Design, calcutate, discuss

Designing steps

• Requirements

Microbes

• Kinetics

Bioreactor types

• Mass Transfer

• Heat Transfer

Dynamics

• Power consumption

• Hold Up

Dimension

Nur Istianah-THP-FTP-UB-2017

FED

BATCH PLUG

FLOW CSTR

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Bioreactor Operation Modes

BATCH

A batch bioreactor is

normally equipped

with an agitator to

mix the reactant, and

the pH of the

reactant is

maintained by

employing either

buffer solution or a

pH controller

Sm

Ss

CK

Cr

dt

dCr

max

trCCC

CK ss

s

sm max0

0ln Change of Cs

with time, t

Batch operation

with stirring

•A foam breaker may be installed to disperse foam

Nur Istianah-THP-FTP-UB-2017

-1.a. Batch Operation

Case study

• Contoh soal 1:

• Sebuah bioreaktor digunakan untuk

memproduksi enzim dengan konsentrasi

substrat awal sebesar 12x10-3 M dan

konversi bioreaktor 90%. Berapakah

waktu yang diperlukan untuk produksi

enzim jika data kinetik reaktor diberikan

sebagai berikut:

• rmax = 2.5 mmol/m3.det; Km = 8.9x10-3 M

Solution

• Konsentrasi substrat akhir,

• Cs = (1-XA)Cs0 = (1-0.9)( 12x10-3 M) = 1.2x10-3 M

• rmax = 2.5 mmol/m3det= 2.5 x10-6 mol/L.det

jamt

t

CCC

CK

rt ss

s

s

m

5.3det12517

x102.1x1012x102.1

x1012ln8.9x10

x105.2

1

ln1

3- 3-

3-

3- 3-

6-

0

0

max

Case study

• Contoh soal 2:

• Hitunglah laju fermentasi etanol dari nira

sorgum jika konsentrasi gula awal sebesar

0.1 M dan di akhir fermentasi diperoleh

konsentrasi gula sebesar 0.025 M.

Fermentasi berlangsung selama 4 jam

dengan konstanta Monod 5x10-3 M.

Solution

• Laju fermentasi bioetanol tersebut adalah 0.017 M.jam-1

1

max

3-

max

0

0

max

.02.0

025.01.0025.0

1.0ln5x10

4

1

ln1

jamMr

r

CCC

CK

tr ss

s

s

m

1

3-

max

.017.0

025.05x10

)025.0(02.0

jamMr

r

CK

Crr

Sm

S

Nur Istianah-THP-FTP-UB-2017

-1.b. Fed Batch Operation

Case study

• Contoh soal 3:

• Sebuah bioreaktor fed batch dioperasikan pada

volume cairan awal 600 mL. Untuk menjaga

kebutuhan nutrisi mikroba didalamnya, telah

dialirkan tambahan media dengan laju alir 0.01

mL/det. Tentukan volume akhir cairan selama

proses fermentasi 2 jam dan tentukan pula

konsentrasi biomas jika pada akhir fermentasi

diperoleh 3.5gr padatan kering(biomass).

Diasumsikan berat molekul biomass sebesar

367 gr/mol.

Solution

Konsentrasi biomass (X) =

mLV

xxmL

mLV

tFVV

tFVV

dtFdV

Fdt

dV

t

t

At

At

A

A

672

det36002det

01.0600

0

0

MX

L

molgr

gr

X

14.0

672.0

.367

5.31

11

In a plug-flow

reactor, the substrate

enters one end of a

cylindrical tube with

is packed with

immobilized enzyme

and the product

steam leaves at the

other end.

max0

0ln rCCC

CK ss

s

s

m

F, Cs0 F, Cs

t = 0 F

V

An ideal plug-flow reactor can

approximate the long tube,

packed-bed and hollow fiber or

multistaged reactor

Residence

time

Continuous

operation without

stirring

V

Nur Istianah-THP-FTP-UB-2017

-2. Plug-flow mode

THANKS FOR YOUR ATTENTION

The best person is one give something useful always

Nur Istianah-THP-FTP-UB-2017