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Umul Fatin. 2016. Induksi Kalus Eksplan Daun Sirih Hitam (Piper betle L.)dengan Kombinasi Konsentrasi Zat Pengatur Tumbuh Indole Butyric Acid (IBA)dan Kinetin. Skripsi ini dibawah bimbingan Dr. Junairiah, S.Si., M.Kes dan Dr. Y.Sri Wulan Manuhara, M.Si., Departemen Biologi, Fakultas Sains dan Teknologi,Universitas Airlangga.

ABSTRAK

Tanaman sirih hitam (Piper betle L.) merupakan tanaman obat yangmemiliki kandungan metabolit sekunder yang berupa alkaloid, flavonoid, saponin,tanin, steroid, triterpenoid dan polifenolat yang berkhasiat sebagai anti infeksi.Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi zat pengaturtumbuh Indole Butyric Acid (IBA) dan kinetin yang paling baik untuk induksikalus sirih hitam (Piper betle L.). Eksplan dari daun P.betle L. ditumbuhkan padamedia Murashige and Skoog (MS) padat yang diperkaya dengan zat pengaturtumbuh IBA dan kinetin dengan konsentrasi masing-masing 0; 0,5; 1; 1,5; 2 mg/L.Panen terhadap kalus dilakukan pada minggu ke-8 usia kultur. Hasil penelitianmenunjukkan bahwa zat pengatur tumbuh IBA dan kinetin berpengaruh terhadappertumbuhan eksplan daun sirih hitam. Penambahan konsentrasi zat pengaturtumbuh IBA 2 mg/L dan kinetin 1,5 mg/L menunjukkan respon terbentuknyakalus paling cepat yaitu 7,5 hari dengan persentase terbentuknya eksplan 100%.Penambahan konsentrasi zat pengatur tumbuh IBA 2 mg/L dan kinetin 1,5 mg/Lmerupakan konsentrasi terbaik menghasilkan berat basah tertinggi yaitu 177 mgdan berat kering tertinggi yaitu 46,6 mg. Warna kalus yang teramati adalah putih,hijau, dan cokelat dengan tekstur kompak.

Kata Kunci :induksi kalus, Piper betle L., IBA, Kinetin

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ADLN - PERPUSTAKAAN UNIVERSITAS AIRLANGGA

SKRIPSI INDUKSI KALUS EKSPLAN... UMUL FATIN

ix

UmulFatin. 2016. Callus Induction of Black Betel’s Leaf (Piper betleL.) Explantwith Combination of Growth Regulators Indole Butyric Acid (IBA) and Kinetin.This script is guided by Dr. Junairiah, S.Si., M.Kes., and Dr. Yosephine SriWulan Manuhara, M.Si., Department of Biology, Faculty of Science andTechnology, Airlangga University, Surabaya.

ABSTRACT

Black betel (Piper betle L.) is medicinal plant which contains secondarymetabolite such as alkaloid, flavonoid, saponin, tannin, steroid, triterpenoid andpolyfenolat that have anti infection ability. This research aimed to know theinfluence of the best combination of growth regulator Indole Butyric Acid (IBA)and kinetin towards callus induction of black betel (Piper betle L.). Explant fromleaf of P.betle L. was grown on solid Murashige and Skoog (MS) mediaaugmented with growth regulators IBA and kinetin with 0; 0,5; 1,0; 1,5; 2,0 mg/Lin concentration respectively. Callus harvesting was done in 8th week of culture.The result of this research showed that IBA and kinetin had effects on explantgrowth of Piper betle L.’s leaf explant. Combination of 2 mg/L IBA and 1,5 mg/Lkinetin showed the fastest callus forming at 7,5 days with 100% in percentage ofexplants forming. Combination of 2 mg/L IBA and 1,5 mg/L kinetin is the bestconcentration which produced the highest fresh weight (177 mg) and the highestdry weight (46,6 mg). The color of callus were white, green and brown withcompact texture

Key words: Callus induction, IBA, Kinetin, Piper betle L.

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