practical manual - fundamentals of plant biochemistry

PRACTICAL MANUAL

Prepared by:

Dr. N.H. GaraniyaDr. A.P. ItaliyaDr. K.H. Patel

Department of Soil Science & Agricultural ChemistryCollege of Agriculture,

Campus BharuchNavsari Agricultural University

FUNDAMENTALS OF PLANT BIOCHEMISTRY

[Biochem 2.1]

Navsari Agricultural University

PRACTICAL MANUAL

Prepared by:

[Biochem 2.1]

PRACTICAL MANUAL

Prepared by:

[Biochem 2.1]

Publisher :PrincipalCollege of Agriculture,Navsari Agricultural UniversityBharuch Compound,Bharuch - 392 012

Edi�on : FIRST

Year : 2018

Funded by : ICAR (Strenthening and development grant 2018-19)

University Publica�on No. : 98/2018-19

Registration No.:________________

CERTIFICATE

This is to certify that the practical exercises duly signed were performed in the

subject of Biochemistry, Course No. Biochem. 2.1 (2+1) [Fundamentals of Plant

Biochemistry] as a part and partial requirement of the Course by

Mr./Ms._________________________________________________

Roll No. _________ of Second Semester class during academic year 20___.

The numbers of practical performed were ______ out of _____.

Course Teacher� � � Professor & Head

Examiner� � � � Examiner

� (Internal Exam.)� � � (External Exam.)

Publisher :PrincipalCollege of Agriculture,Navsari Agricultural UniversityBharuch Compound,Bharuch - 392 012

Edi�on : FIRST

Year : 2018

Funded by : ICAR (Strenthening and development grant 2018-19)

University Publica�on No. : 98/2018-19

Registration No.:________________

CERTIFICATE

This is to certify that the practical exercises duly signed were performed in the

subject of Biochemistry, Course No. Biochem. 2.1 (2+1) [Fundamentals of Plant

Biochemistry] as a part and partial requirement of the Course by

Mr./Ms._________________________________________________

Roll No. _________ of Second Semester class during academic year 20___.

The numbers of practical performed were ______ out of _____.

Course Teacher� � � Professor & Head

Examiner� � � � Examiner

� (Internal Exam.)� � � (External Exam.)

Title of Practical Page

Date Signature

1 Quantitative determination of total carbohydrates from

plant material by Anthrone Method

2 Estimation of soluble proteins from plant leaf or grain

samples by Lowry's Method

3 To observe protein denaturation by various factors 7

4 Estimation of Free Fatty Acids from Oil/Fat samples 10

5 Estimation of Total Phenol content from Plant samples 12

6 Estimation of titrable acidity from different samples 14

7 Estimation of Vitamin-C by titrametric method 18

8 Estimation of Chlorophyll ‘a’ and ‘b’ content in green

leaves

9 Extraction of Plant Genomic DNA by CTAB Method 22

10 Quantification of Plant Genomic DNA 25

Title of Practical Page

Date Signature

1 Quantitative determination of total carbohydrates from

plant material by Anthrone Method

2 Estimation of soluble proteins from plant leaf or grain

samples by Lowry's Method

3 To observe protein denaturation by various factors 7

4 Estimation of Free Fatty Acids from Oil/Fat samples 10

5 Estimation of Total Phenol content from Plant samples 12

6 Estimation of titrable acidity from different samples 14

7 Estimation of Vitamin-C by titrametric method 18

8 Estimation of Chlorophyll ‘a’ and ‘b’ content in green

leaves

9 Extraction of Plant Genomic DNA by CTAB Method 22

10 Quantification of Plant Genomic DNA 25

practical manual - fundamentals of plant biochemistry

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