Vol 17, No 3 September 2016 · Vol 17, No 3 September 2016 Terakreditasi Dirjen Dikti S.K. No....

Vol 17, No 3 September 2016

Terakreditasi Dirjen Dikti

S.K. No. 81/DIKTI/Kep/2011

pISSN: 1411-8327; eISSN: 2477-5665Website : ojs.unud.ac.id

Jurnal Tiga BulananJurnal Veteriner

Jurnal Kedokteran Hewan Indonesia

(Terbit : Maret, Juni, September, Desember)

depan 1

PEMIMPIN UMUM : I WAYAN BATAN DEWAN REDAKSI : NYOMAN MANTIK ASTAWA (KETUA), IDA BAGUS ARKA,NYOMAN SADRA DHARMAWAN, IWAN H. UTAMA, I GUSTI NGURAH KADE MAHARDIKA, I KETUT PUJA, I KETUTSUATHA, TJOK GDE OKA PEMAYUN, ROOSTITA L. BALIA, I KETUT BERATA, REDAKTUR PELAKSANA : I NYOMANSUARTHA & I G M KRISNA ERAWAN, SEKRETARIS REDAKSI : I NYOMAN SUARSANA, STAF REDAKSI : I WAYANSUARDANA, I GUSTI NGURAH SUDISMA, AIDA LOUISE TENDEN ROMPIS, NI GUSTI AGUNG AYU SUARTINI, I MADE SUKADA,ANAK AGUNG SAGUNG KENDRAN, ANAK AGUNG AYU MIRAH ADI, I MADE KARDENA, YANA QOMARIANA.TATA USAHA : PUDJI RAHARDJO, WERDI SUSARI. KANTOR REDAKSI & ALAMAT SURAT : KLINIK HEWAN FKH UNUD,Jl. Raya Sesetan Gg. Markisa 6 Banjar Gaduh, Denpasar - Bali. PENERBIT : FAKULTAS KEDOKTERAN HEWAN, UNIVERSI-TAS UDAYANA BEKERJA SAMA DENGAN PERHIMPUNAN DOKTER HEWAN INDONESIA. REKENING : NOMOR 0118628705A/N drh. I NYOMAN SUARTHA, M.Si BNI CABANG DENPASAR DICETAK OLEH: PERCETAKAN PELAWA SARI, JL. ANTOSURA33 DENPASAR

Setiap naskah yang dikirim ke redaksi untuk dipublikasikan dalam

Jurnal Veteriner dipandang sebagai karya asli penulis dan bila diterima, naskah

tersebut tidak diperkenankan dipublikasikan lagi secara keseluruhan ataupun

sebagian tanpa seijin Jurnal Veteriner.

Di Kalimantan berdasarkan wilayahnya tersebar tiga subspesies orang utan. Orang utan subspecies

Pongo pygmaeus pygmaeus ada di barat laut; Pongo pygmaeus wurmbii ada di barat dan di tengah; dan

Pongo pygmaeus morio ada di timur. Status mereka pada tahu 2016 dinyatakan kritis (terancam punah)

oleh Badan Konservasi Dunia, mengikuti saudaranya orang utan Sumatra Pongo abelii. Yang membuat

mereka nyaris punah, karena kebanyakan dari mereka hidup di kawasan non konservasi yang ditetapkan

sepihak oleh manusia. Mereka kini hidup pada hutan produksi, konsesi batubara, konsesi kelapa sawit,

kebun warga, dan sempadan sungai. Mereka renta berkonflik dengan saudara satu kelasnya, manusia

(Homo sapiens). Oleh manusia yang beriman (?) mereka biasa dijebak, diperangkap, ditangkap, dan

diperdagangkan. Walau pun sebenarnya kita sudah memberi perhatian kepada mereka, nampaknya apa

yang telah kita berikan, sepertinya itu semua belum cukup.A

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KerjasamaFakultas Kedokteran Hewan Universitas Udayana& Perhimpunan Dokter Hewan Indonesia, Jakarta

MITRA BESTARI JURNAL VETERINER

Prof. Dr. drh. Sri Subekti DEAGuru Besar Parasitologi Fakultas Kedokteran Hewan Universitas Airlangga Surabaya

Prof. drh. Hastari Wuryastuti,MSc., PhDGuru Besar Nutisi Klinik Fakultas Kedokteran Hewan Universitas Gadjah MadaYogyakarta

Prof. drh. R. Wasito,MSc., PhDGuru Besar Patologi Fakultas Kedokteran Hewan Universitas Gadjah MadaYogyakarta

Prof. Ir. D.K.HaryaPutra,MSc.,PhD.Guru Besar Biologi Reproduksi Fakultas PeternakanUniversitas Udayana Denpasar

Prof. Dr. I Wayan Teguh WibawanAhli Imunologi, Mikrobiologi, Patologi Fakultas Kedokteran Hewan Institut Pertanian Bogor

Drh. Dewa Made Ngurah Dharma,MSc., PhD.Ahli Patogi Balai Besar Veteriner Denpasar

Prof. Dr. Ir Ika MustikaProfesor Riset, Ahli Nematologi Balai Penelitian Tanaman Tropis, Cimangu Bogor

Prof. Ir. Wasmen Manalu,PhDGuru Besar Fisiologi dan Farmakologi Fakultas Kedokteran Hewan IPB Bogor

Prof. drh. Adji Santoso Dradjat, BSc Vet. Mphil., PhD.Guru Besar Reproduksi Ternak Fakultas Peternakan Universitas Mataram

Prof (Riset) Dr Supar, MS, APUAhli Bakteriologi Balai Besar Penelitian Veteriner Bogor

Dr Nastiti KusumoriniAhli Neurofisiologi Fakultas Kedokteran Hewan IPB Bogor

Drh Fadjar Satrija, MSc., PhDAhli Parasitologi Veteriner Fakultas Kedokteran Hewan IPB Bogor

Prof. Dr. Fedik Abdul RatamGuru Besar Virologi dan Imunologi Fakultas Kedokteran Hewan Universitas Airlangga

Prof . Dr. drh. Siti Isrina Oktavia salasiaGuru Besar Patologi Klinik Fakultas Kedokteran Hewan Universitas Gadjah Mada Yogyakarta

Drh. Sulistiyani, MSc., PhDAhli Molekular dan Selular Patobiologi Fakultas MIPA IPB Bogor

Prof. drh Arief Boediono, PhDGuru Besar Embriologi Fakultas Kedokteran Hewan IPB Bogor

Dr. Agus WiyonoAhli Virologi, Sub Direktorat Perlindungan Hewan Dirjenak Deptan Jakarta

Naskah AsliOriginal Article

WYANDA ARNAFIA, SITI GUSTI NINGRUM, ERFIANDINI EKA PUSPITA,DENNY WIDAYA LUKMAN, FACHRIYAN HASMI PASARIBU, I WAYAN TEGUH WIBAWANCross Reaction of Serum in Salmonella enteritidis-Vaccinated Chicken to Some Salmonella enterica Serotypes(REAKSI SILANG SERUM AYAM YANG DIVAKSIN DENGAN SALMONELLA ENTERITIDIS TERHADAP BEBERAPA SEROTIPE SALMONELLA ENTERICA) ............................ 316-321

RISA INDRIANI, NI LUH PUTU INDI DHARMAYANTIPengembangan Vaksin Inaktif TeteloGenotipe VII Isolat Lokal pada Kondisi Laboratorium.(DEVELOPMENT OF TETELO INACTIVATED VACCINEGENOTYPE VII LOCAL ISOLATE IN LABORATORY CONDITION) ................................................... 322-330

ANDIKA BUDI KURNIANTO, GUSTI AYU YUNIATI KENCANA,I NYOMAN MANTIK ASTAWARespons Antibodi Sekunder Terhadap Penyakit Tetelo pada Ayam Petelur PascavaksinasiUlangan dengan Vaksin Tetelo Aktif(NEWCASTLE DISEASESECONDARY ANTIBODY RESPONSEAFTER REVACCINATION IN LAYER WITH THE ACTIVE ND VACCINE) ........................................ 331-336

WAHONO ESTHI PRASETYANINGTYAS, DENY PUTRA ROMADHON, FITRI SUSANA,ITA DJUWITA, KUSDIANTORO MOHAMADBlack Seed (Nigella sativa) Extract Induce in Vitro Proliferationand Differentiation of Rat Pancreatic and Bone Cells(EKSTRAK JINTAN HITAM (Nigela sativa) MENGINDUKSI PROLIFERASIDAN DIFERENSIASI SEL PANKREAS DAN SEL TULANG TIKUS SECARA IN VITRO) ................. 337-346

WORO HASTUTI SATYANTINI, AGUSTONO, ARIMBI,EMY KOESTANTI SABDONINGRUM, MYRNA BUDI, LINA WAFIA ASMIPeningkatan Respons Imun Non Spesifik Ikan Gurame PascapemberianEkstrak Air Panas Mikroalga Spirulina platensis(ENHANCEMENT OF NON-SPECIFIC IMMUNE RESPONSE OF OSPHRONEMUS GOURAMYAFTER GIVING OF HOT WATER EXTRACT SPIRULINA PLATENSIS) ............................................. 347-354

KHAIRUN NISAA, SUKENDA, MUHAMMAD ZAIRIN JUNIOR,ANGELA MARIANA LUSIASTUTI, SRI NURYATIBenih Keturunan Induk Ikan Nila yang Divaksinasi pada Tingkat Kematangan Gonad-2Lebih Tahan Terhadap Infeksi Streptococcus agalactiae(RESISTANCE OF FRY FROM TILAPIA (OREOCHRIMIS NILOTICUS) VACCINATIONBROODSTOCK AT DIFFERENT GONADAL DEVELOPMENTAL STAGESTO STREPTOCOCCUS AGALACTIAE INFECTION) ................................................................................ 355-364

NINING ARINI, MIRNAWATI SUDARWANTO, IDWAN SUDIRMAN, AGUSTIN INDRAWATIPemanfaatan Supernatan Lactobacillus plantarum Sebagai PenghambatPertumbuhan Escherichia coli pada Dangke Susu Sapi(UTILIZATION OF LACTOBACILLUS PLANTARUM SUPERNATANAS AN INHIBITIOR OF ECHERICHIA COLI GROWTH IN COW’S MILK DANGKE .......................... 365-373

KORBINIANUS FERIBERTUS RINCA, TJOKORDA SARI NINDHIA,I WAYAN SUARDANAFaktor-faktor Risiko Penyebaran Escherichia coli O157:H7pada Sapi Bali di Kuta Selatan, Badung, Bali(RISK FACTORS FOR DISSEMINATION OF ESCHERICHIA COLI O157:H7IN BALIN CATTLE IN SOUTH KUTA, BADUNG, BALI) ......................................................................... 374-382

TRI NURHAJATI, KOESNOTO SOEPRANIANONDO,WIDYA PARAMITA LOKAPIRNASARIUji Aktivitas Pertumbuhan Enterobacter cloacae Selulolitik Aerob Rumen-1 IsolatAsal Limbah Cairan Rumen Sapi Peranakan Ongole(GROWTH ACTIVITY ASSAY OF CELLULOLYTIC BACTERIA ENTEROBACTER CLOACAESAR 1 (CELLULOLYTIC AEROB RUMEN 1) ISOLATED FROM ONGOLE CROSSBREEDBOVINE RUMEN FLUID WASTE) ................................................................................................................. 383-388

I GUSTI AYU DEWI RATNAYANTI, NI PUTU SRIWIDYANI, I DEWA AYU INTEN PRIMAYANTI,I GUSTI KAMASAN, NYOMAN ARIJANA, I GUSTI NYOMAN SRI WIRYAWAN,IDA AYU IKA WAHYUNIARI, I WAYAN SUGIRITAMA, I GUSTI NGURAH MAYUNGrowth Hormone Menurunkan Ekspresi Protein p53 dan p21 Sel Endotel Tikus Jantan(GROWTH HORMONE REDUCES P53 AND P21 ENDOTHELIALPROTEIN EXPRESSION IN MALE RAT) .................................................................................................... 389-395

DAFTAR ISIVol 17, No. 3 September 2016

Terakreditasi Dirjen DiktiS.K. No. 81/DIKTI/Kep/2011


Terbit sejak 18 Desember 2000

Jurnal VeterinerJurnal Kedokteran Hewan Indonesia Indonesian Veterinary Journal

depan 3

DAFTAR ISI (Lanjutan)Vol 17, No 3 September 2016






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NOVI SUPRIHATIN, LIGAYA ITA TUMBELAKA, MOHAMAD AGUS SETIADIProfil Progesteron Air Susu dan Tingkat Kebuntingan Sapi Perah PascasinkronisasiEstrus Menggunakan Prostaglandin F

2Alfa atau Progesteron-CIDR

(MILK PROGESTERONE PROFILE AND PREGNANCY RATE ON DAIRY CATTLEAFTER ESTROUS SYNCHRONIZATION WITH PROSTAGLANDIN F

2

ALFA OR PROGESTERONE-CIDR) .............................................................................................................. 396-403

RINI WIDYASTUTI, SONY HERU SUMARSONO, ARIEF BOEDIONO, SITI DARODJAH RASADLow Concentration of Ethylene Glycol Improve Recovery Rate of HumanSpermatozoa After Vitrification(ETILEN GLIKOL KONSENTRASI RENDAH MENINGKATKANRECOVERY RATE SPERMATOZOA MANUSIA PASCAVITRIFIKASI) ................................................. 404-410

YULIANA, SIANNY HERAWATIPhytochemical Content and Protective Effect of Kleinhovia hospital Leaves Extracton Pancreatic Cytotoxicity in Hyperglycemic Rats(KANDUNGAN FITOKIMIA DAN EFEK PROTEKTIF EKSTRAK DAUN PALIASA (KLEINHOVIAHOSPITA Linn) PADA SITOTOKSISITAS PANKREAS TIKUS HIPERGLIKEMIA) ........................... 411-417

ISWINARNO DOSO SAPUTRO, LOBREDIA ZARASADE, REVITA WIDYA PRASANTIHambatan Kolonisasi Methicillin Resistant Staphylococcus Aereus oleh EkstrakKulit Delima pada Luka Bakar Derajat-2 pada Tikus(INHIBITION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS BACTERIACOLONIZATION ON SECOND DEGREE BURN WOUND IN WISTAR RATBY USING POMEGRANATE PEEL EXTRACT) ......................................................................................... 418-423

IWAN HARJONO UTAMA, SRI KAYATI WIDYASTUTI, ANAK AGUNG AYU MIRAH ADI,I GUSTI MADE KRISNA ERAWAN, IDA BAGUS OKA WINAYA, IDA BAGUS KOMANG ARDANA,ACHOIRO WATI RASID, TYAS PANDIEKA YOGAGambaran Sitologi Cairan Peritoneal dan Sinovial Itik Bali(CYTOLOGIC FIGURE OF PERITONEAL AND SYNOVIAL FLUID IN BALI DUCKS) ..................... 424-429

DILLA MAREISTIA FASSAH, LILIS KHOTIJAHPengimbuhan Vitamin-E dalam Ransum Kaya Asam Lemak Tidak JenuhTerhadap Profil Darah Induk Domba Laktasi§

(VITAMIN-E SUPPLEMENTATION ON RICH POLY-UNSATURATEDFATTY ACID RATION TO BLOOD PROFILE OF LACTATION EWES) ............................................... 430-439

HILMA PUTRI FIDYANDINI, MUNTI YUHANA, ANGELA MARIANA LUSIASTUTIPemberian Probiotik Multispesies dalam Media Budi Daya Ikan Lele Dumbo untukMencegah Penyakit Motile Aeromonads Septicemia(ADDITION OF MULTISPECIES PROBIOTICS IN THE CULTURE MEDIUM OF AFRICANCATFISH TO PREVENT THE MOTILE AEROMONADS SEPTICEMIA DISEASE) .......................... 440-448

ANINDYA KURNIAWATI, DENNY WIDAYA LUKMAN, I WAYAN TEGUH WIBAWANResistensi Antibiotik pada Salmonella Isolat Sapi Bakalan Asal Australia yang DiimporMelalui Pelabuhan Tanjung Priok Jakarta(ANTIBIOTIC RESISTANCE OF SALMONELLA ISOLATES FROM AUSTRALIAN IMPORTEDFEEDER CATTLES THROUGH TANJUNG PRIOK PORT JAKARTA) ................................................ 449-456

MUHAMMAD RIZA, MUHAMMAD RIYADHIFertilitas Semen Kerbau Rawa (Bubalus bubalis carabanensis) yang Diencerkandengan Pengencer Nira Aren(FERTILITY OF SWAMP BUFFALO SEMEN (BUBALUS BUBALIS CARABANENSIS)DILUTED WITH SUGAR PALM JUICE EXTENDER) ............................................................................... 457-467

DATIK SETIAWATI, BAMBANG SUKAMTO, HANNY INDRAT WAHYUNIPengimbuhan Enzim Fitase dalam Ransum Ayam Pedaging Meningkatkan PemanfaatanKalsium untuk Pertumbuhan Tulang dan Bobot Badan(ADDING PHYTASE ENZYMES ON BROILER RATION INCREASING CALCIUM UTILIZATIONFOR BONE GROWTH AND BODY WEIGHT GAIN) ................................................................................ 468-476

PEDOMAN BAGI PENULIS ................................................................................................................................... i-ii

Mitra Bebestari Maret 2015

Indrawati SendowBalai Besar Penelitian Veteriner (Balitvet) jln REMartadinata No 30, Bogor 16114

Prof Dr Siti Isrina Oktavia SalasiaLab Patologi Klinik Fakultas Kedokteran HewanUniversitas Gadjah Mada Jln Fauna No 2 KampusUGM Jogjakarta

Dr Rini WidayantiBagian Biokimia Fakultas Kedokteran HewanUniversitas Gajah Mada Jln Fauna No 2 Kampus UGMJogjakarta 55281

Dr Drh I Wayan Suardana MSiLab Kesehatan Masyarakat Veteriner FakultasKedokteran Hewan Universitas Udayana

Drh Cok Sari Nindhia MPLab Biostatistika Fakultas Kedokteran HewanUniversitas Udayana, Jln Sudirman Denpasar Bali

Prof Dr Drh Retno Damajanti MSLab Imunologi, Fakultas Kedokteran Hewan InstitutPertanian Bogor Jln Agatis Kampus IPB DramagaBogor 16680

Prof Wasmen Manalu PhDLab Fisiologi, Dept AFF, Fakultas Kedokteran HewanInstitut Pertanian Bogor, Jln Agatis Kampus IPBDramaga Bogor 16680

Dr Indrawati SendowBalai Besar Penelitian Veteriner (Balitvet) JLN REMartadinata No 30, Bogor 16114

Drh I Made Kardena MVScLab Patologi Fakultas Kedokteran Hewan UniversitasUdayana, Jln Sudirman Denpasar Bali

Dr Bayu RosadiFakultas Peternakan Universitas Jambi Jln JambiMuaro Bulian Km 15 Mandalo Darat Jambi

Prof Dr HerdisBadan Pengkajian dan Penerapan Teknologi (BPPT)Gedung II BPPT Lt.16 Jalan M.H. Thamrin No.8Jakarta Pusat 10340

Dr drh Arman SayutiLab Farmakologi, Fakultas Kedokteran HewanUniversitas Syiah Kuala, Jln Tengku Hasan KruengKalee No 4 Kampus Unsyiah, Darusalam Banda AcehNangroe Aceh Darusalam 23111

Prof Dr drh Tongku Nizwan Siregar MPBagian Reproduksi dan KebidananFakultas KedokteranHewan Universitas Syiah Kuala, Jln Tengku HasanKrueng Kalee No 4 Kampus Unsyiah, DarusalamBanda Aceh Nangroe Aceh Darusalam 23111

Prof drh Deni Noviana PhDBagian Bedah dan Radiologi, Departemen Klinik,Reproduksi dan Patologi, Fakultas Kedokteran Hewan,Institut Pertanian Bogor, Bogor, 16680, Tlp. (0251)8628080

MITRA BEBESTARI TAMU JURNAL VETERINER 2015 (1 dari 5)

Kami mengucapkan terima kasih dan penghargaan setinggi-tingginya kepada para mitra bestari JurnalVeteriner yang tercantum di bawah ini atas, kesediaannya dan ketekunannya menelaah dengan penuh

seksama artikel-artikel yang dikirim ke kami, sehingga artikel tersebut menjadi jauh lebih sempurna.Ada pun mitra bebestari Jurnal Veteriner untuk edisi tahun 2015 adalah :

Dr Muhammad RizalKompleks Perumahan Meranti Griya Oxygen JlnOxygen 1 Blok D No 8 Kota Banjar Baru KalimantanSelatan 70711

Prof Dr drh Iman SupriatnaBagian Reproduksi dan KebidananDept Klinik Patologidan Reproduksi Fakultas Kedokteran Hewan, InstitutPertanian Bogor Kampus IPB Dramaga, Jln AgatisBogor 16680

Prof Drh Adji Santoso Dradjat MSc, PhDFakultas Peternakan, Universitas Mataram JlnMajapahit No 62 Mataram NTB

dr Yuliana M.BiomedLab Anatomi, Fakultas Kedokteran UniversitasUdayana, Jln Sudirman, Denpasar, Bali

Dr drh Gunanti, MSBagian Bedah dan Radiologi, Departemen Klinik,Reproduksi dan Patologi, Fakultas Kedokteran Hewan,Institut Pertanian Bogor, Bogor, 16680, Tlp. (0251)8628080

Prof drh Anak Agung Ayu Mirah Adi, MSi, PhDLab Patologi, Fakultas Kedokteran Hewan, UniversitasUdayana, Jln Sudirman Denpasar Bali

Dr drh Hera MaheswariLab Fisiologi, Dept AFF, Fakultas Kedokteran HewanInstitut Pertanian Bogor, Jln Agatis Kampus IPBDramaga Bogor 16680

Dr Agustina Dwi WijayantoBagian Farmakologi, Fakultas Kedokteran Hewan,Universitas Gadjah Mada, Kampus UGM, Jln FaunaNo 2 Jogjakarta 55281

Prof drh M Lazuardi, MSc, PhDBagian Farmasi, Fakultas Kedokteran HewanUniversitas Airlangga, Kampus-C Unair, JlnMulyorejo, Surabaya

Dr I Putu Sampurna MSLab Biostatistika Fakultas Kedokteran HewanUniversitas Udayana, Jln Sudirman Denpasar Bali

Dr Ir Bagus Priyo Purwanto, MAgrDepartemen Ilmu Produksi dan Teknologi PeternakanFakultas Peternakan, Institut Pertanian Bogor,KampusDramaga IPB, Jln Agathis, Bogor 16680

Ir Ari Sudewo MSFakultas Peternakan, Universitas Jendral Sudirman JlnSoeparno Karang Wangkal, Purwekerto 53123

Prof Dr Nono NgadionoFakultas Peternakan, Universitas Gadjah Mada, JlnFauna No 3, Kampus UGM, Jogjakarta, 55281

Prof Wasmen Manalu PhDLab Fisiologi, Dept AFF, Fakultas Kedokteran HewanInstitut Pertanian Bogor, Jln Agatis Kampus IPBDramaga Bogor 16680

Dr I Putu Sampurna MSLab Biostatistika Fakultas Kedokteran HewanUniversitas Udayana, Jln Sudirman Denpasar Bali

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Dr Theresa Nur Indah KoniJurusan Peternakan, Politeknik Pertanian NegeriKupang Jln Adi Sucipto, Penfui, Kupang, NusaTenggara Timur

Dr Mirni LamidDepartemen Peternakan, Fakultas Kedokteran HewanUniversitas Airlangga, Kampus-C Unair, JlnMulyorejo Surabaya

Prof I Made Mastika MSc, PhDFakultas Peternakan Universditas Udayana JlnSudirman Denpasar Bali

Prof Dr Cece SumantriLab. Genetika dan Pemulian Ternak, Departemen IlmuProduksi dan Teknologi Peternakan, FakultasPeternakan, Institut Pertanian Bogor, KampusDramaga IPB Jln Agatis Bogor 16680

Dr drh Wiwin Winarsih, MSiBagian Patologi, Fakultas Kedokteran Hewan InstitutPertanian Bogor, Kampus IPB Dramaga Jln AgatisBogor 16680

Dr drh Wisnu NurcahyoLab Parasitologi, Fakultas Kedokteran HewanUniversitas Gadjah Mada, Jln Fauna No 2, KampusUGM, Jogjakarta

Dr Drh I Nyoman Suartha MSiLab Penyakit Dalam Veteriner Fakultas KedokteranHewan Universitas Udayana


Prof drh Nyoman Suarsana, MSiLab Biokimia, Fakultas Kedokteran Hewan,Universitas Udayana, Jln Sudirman Denpasar Bali

Mitra Bebestari Juni 2015

Drh I Ketut Adnyane Mudite, PhDLab Histologi, Fakultas Kedokteran Hewan InstitutPertanian Bogor Jln Agatis Kampus IPB DramagaBogor 16680


Prof Dr Drh Fachriyan H PasaribuLab Mikrobiologi Medik, Fakultas KedokteranHewan, Institut Pertanian Bogor Jln Agathis KampusIPB Dramaga Bogor 16680


Dr drh Maxs Urias Ebenhaizar Sanam MScFakultas Kedokteran Hewan, Univesitas Nusa CendanaJln Adi Sucipto, Penfui, Kupang, NTT 85001

Dr drh AAG PutraBalai Besar Veteriner Denpasar Jln Raya SesetanPegok, Denpasar

Dr Drh I Nyoman Suartha MSiLab Penyakit Dalam Veteriner Fakultas KedokteranHewan Universitas Udayana

Dr Inriyanti AssaFakultas Kesehatan Masyarakat UniversitasCendrawasih Jln Sentani Abepura Kampus BawahAbepura Jaya Pura, Papua

Dr Drh. Elok Budiretnani MS.Bag/Lab Helminthologi FKH IPB. Bidang Keahlian :Helminthologi

Prof Dr Putu SutisnaFakultas Kedokteran, Universitas Warmadewa JlnTerompong Denpasar Bali

Dr Drh. Elok Budiretnani MS.Bag/Lab Helminthologi FKH IPB. Bidang Keahlian :Helminthologi Fakultas Kedokteran Hewan, InstitutPertanian Bogor, Kampus IPB Dramaga

Dr Inriyanti AssaFakultas Kesehatan Masyarakat UniversitasCendrawasih Jln Sentani Abepura Kampus BawahAbepura Jaya Pura, Papua

Prof Dr Drh NS Dharmawan MSLab Patologi Klinik Veteriner Fakultas KedokteranHewan Universitas Udayana

Prof Dr Sri Subekti Bandryman SoedjokoLab Parasitologi, Fakultas Kedokteran HewanUniversitas Airlangga, Kampus-C Unair Jln Mulyorejo,Surabaya

Dr drh Angela Mariana Lusiastuti MSiInstalasi Litbang Pengendalian Penyakit Ikan JlnPerikanan No 13 Depok

Dr Woro Hastuti SatyantiniDept Manajemen Kesehatan Ikan dan BudidayaPerairan, Fakultas Perikanan dan Kelautan, UniversitasAirlangga, Kampus-C Unair, Jln Mulyorejo Surabaya

Dr Ni Nyoman Suryani MSiFakultas Peternakan, Universitas Udayana, JlnSudirman, Denpasar, Bali

Prof Dr Edy Rianto MScLab Produksi Ternak Potong dan Ternak Perah FakultasPeternakan dan Pertanian, Universitas Diponogoro JlnKampus drh Soejono Kusumowardoyo TembalangSemarang Jawa Tengah 50275

Prof Dr Asmuddin Natsir MScKompleks Universitas Hasanuddin (Unhas) Blok GBNo 28, Tamalanrea, Makassar 90245

Sri Sugiwati, S.Si, M.SiTaman Sari Bukit Damai Blok A4 No.18, GunungSindur Kab. Bogor Jawa Barat 16340

Dra Puspa Dewi N LotulungLab Kimia Terapan LIPI, Kompleks Puspitek Serpong,Tanggerang, Banten




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Drh Wirdateti, MSiBidang Zoologi, Pusat Penelitian Biologi Lembaga IlmuPengetahuan Indonesia / LIPI Jln Raya Jakarta-Bogorkm 46, Cibinong, Bogor

Prof Dr Drh Mohammad Agus SetiadiBagian Reproduksi dan Kebidanan, Dept Klinik,Reproduksi dan Patologi, Fakultas Kedokteran Hewan,Institut Pertanian Bogor,Jln Agattis Kampus IPBDramaga 16880

Prof Dr Aulani’am, DESDepartemen Kimia, Fakultas Matematika dan IlmuPengetahuan Alam, Universitas Brawijaya, Jln. VeteranMalang

Dr Gatot CiptadiFakultas Peternanak, Universitas Brawijaya JlnVeteran Malanag Jawa Timur

Dr Drh Widjiati MsiFakultas Kedokteran Hewan Universitas Airlangga,Kampus C Unair Jln Mulyorejo, Surabaya

Drh Ni Wayan Kurniani Karja PhDBagian Reproduksi dan Kebidanan, Dept Klinik,Reproduksi dan Patologi Fakultas Kedokteran Hewan,Institut Pertanian Bogor, Kampus IPB Dramaga

Dr Drh Tjok Gede Oka PemayunLab Reproduksi Dan Kebidanan Fakultas KedokteranHewan Universitas Udayana Jln Sudirman DenpasarBali

Prof Dr Raden Iis ArifiantiPS Biologi Reproduksi, Bagian Reproduksi danKebidanan Departemen Anatomi FisiologiFarmakologi, Fakultas Kedokteran Hewan, InstitutPertanian Bogor, Jalan Agatis Kampus IPB DarmagaBogor 16680

Prof Dr drh Pudji AstutiLab Fisiologi, Fakultas Kedokteran HewanUniversitasGadjah Mada, Jln Fauna No 2, Kampus UGMJogjakarta

Prof Dr Drh Mohammad Agus SetiadiBagian Reproduksi dan Kebidanan, Dept Klinik,Reproduksi dan Patologi, Fakultas Kedokteran Hewan,Institut Pertanian Bogor, Jln Agattis Kampus IPBDramaga 16880

Dr Thomas Mata HineLab Biologi Reproduksi & Kesehatan Ternak, FakultasPeternakan Universitas Nusa Cendana, Jln Adi Sucipto,Penfui, Kupang, NTT-85000

Dr Mobius Tanari MSiFakultas Pertanian, Universitas Tadulako Jln Soekarno-Hatta km-9, Bumi Tadulako, Tondo Palu SulawesiTengah

Dr Dewi Elfidasari MsiLP2M UAJ Jln Sisingamanagaraja, Kebayoran Baru,Jakarta Selatan, 12110

Dr drh Yatri Dr estini MScBagian Kesehatan Masyarakat Veteriner FakultasKrdokteran Hewan Universitas Gadjah Mada Jln FaunaNo 2 Kampus UGM Jogjakarta

Dr drh Tri UntariBagian Mikrobiologi Fakultas Kedokteran HewanUniversitas Gadjah Mada Jl. Fauna 2, Karangmalang,Yogyakarta 55281

Mitra Bebestari September 2015

Dr drh Wisnu NurcahyoLab Parasitologi, Fakultas Kedokteran HewanUniversitas Gadjah Mada, Jln Fauna No 2, KampusUGM, Jogjakarta

Prof Dr drh Rochiman Sasmita MSLab Parasitologi Fakultas Kedokteran HewanUniversitas Airlangga, Kampus-C Unair Jln Mulyorejo,Surabaya

Dr drh Ida Ayu Pasti Apsari MPLab Parasitologi Fakultas Kedokteran HewanUniversitas Udayana Jln Sudirman Denpasar Bali


Prof drh Fadjar Satrija PhDLab Helmintologi, Fakultas Kedokteran Hewan,InstitutPertanian Bogor, Kampus IPB Dramaga Jln AgatisBogor 16680

Dr Welmintje Marlena Messang Nalley.Lab Reproduksi Dan Kesehatan Hewan, FakultasPeternakan, Universitas Nusa Cendana

Prof Trini SusilawatiLab Reproduksi Hewan Fakultas Peternakan,Universitas Brawijaya, Jln Veteran Malang

Dr Muhammad RizalKompleks Perumahan Meranti Griya Oxygen JlnOxygen 1 Blok D No 8 Kota Banjar Baru KalimantanSelatan 70711

Dr Endang SSWBalai Besar Pelatihan Peternakan Batu Jln SonggoritiNo 24 Batu Jawa Timur

Dr Widya Ayu PuspitaJln Wiguna Selatan II/17, Surabaya

Dr Drh I Wayan Suardana MSiLab Kesehatan Masyarakat Veteriner FakultasKedokteran HewanUniversitas Udayana

Dr drh Yusuf Ridwan MSiBagian Parasitologi Dept Ilmu Penyakit Hewan danKesmavet Fakultas Kedokteran Hewan InstitutPertanian Bogor, Jln Agatis Bogor 16680

Dr Ietje WientarsihLab Farmasi, Dept Klinik Patologi Reproduksi,Fakultas Kedokteran Hewan,Institut Pertanian BogorJln Agathis Kampus IPB Dramaga Bogor 16680




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Dr Adriani, MSiFakultas Peternakan, Universitas Jambi Jln JambiMuaro Bulian km 15 Mandalo Darat Jambi

Prof Dr Revolson A Mege, MSJurusan Biologi Fakultas Matematika IlmuPengetahuan Alam, Universitas Negeri Manado,Manado Sulawesi Utara


Dr Soewarno Hadisusanto SULab Ekologi, Fakultas Biologi, Univ Gadjah Mada JlnTeknika Seltan, jogjakarta 55281

Prof Dr drh Darmawi MSiFakultas Kedokteran Hewan Universitas Syiah KualaJln Tgk Hasan Krueng Kalee No 4 Darusalam, BandaAceh 23111

Dr drh I Nyoman Dibia MPBalai Besar Veteriner Denpasar Jln Raya Sesetan,Pegok, Sesetan Denpasar Bali

Dr dr I Dew Made Sukrama, MSi, SpMKBagian Mikrobiologi, Fakultas Kedokteran,Universitas Udayana, Jln Sudirman, Denpasar, Bali

Prof Dr Ida Bagus Putra Manuaba MphilBagian Kimia, Fakultas Matermatika dan IlmuPengetahuan Alam, Universitas Udayana, KampusUnud Bukit Jimbaran, Kuta, Badung, Bali

Dr NursyirwaniLab Mikrobiologi Laut, Jurusan Ilmu Kelautan,Fakultas Perikanan dan Ilmu Kelautan, UniversitasRiau, Kampus Bina Widya Km 12,5 Simpang Baru,Panam, Pekan Baru 28239

dr Ni Nengah Dwi Fatmawati, SpMK, PhDLab Mikrobiologi Klinik Fakultas Kedokteran,Universitas Udayana, Jln Sudirman, Denpasar, Bali

Prof Dr Cece SumantriLab. Genetika dan Pemulian Ternak, Departemen IlmuProduksi dan Teknologi Peternakan, FakultasPeternakan, Institut Pertanian Bogor, KampusDramaga IPB Jln Agatis Bogor 16680

Drh Hamny MSiLaboratorium Anatomi Fakultas Kedokteran HewanUniversitas Syiah Kuala, Banda Aceh Jln Tgk HasanKrueng Kalee No 4 Darusalam, Banda Aceh 23111

Dr Despal MSc AgrFakultas Peternakan, Institut Pertanian Bogor JlnAgatis, Kampus IPB, Dramaga Bogor 16680

Prof Dr Ristiyanto Utomo MSFakultas Peternakan,Universitas Gadjah Mada JlnFauna No 3 Kampus UGM, Jogjakarta

Prof Dr Asmuddin Natsir MScKompleks Universitas Hasanuddin (Unhas) Bolk GBNo 28, Tamalanrea, Makassar 90245

dr Yuliana M.BiomedLab Anatomi, Fakultas KedokteranUniversitasUdayana, Jln Sudirman, Denpasar, Bali

Mitra Bebestari Desember 2015

Dr MasrianiProgram Studi Pendidikan Biologi, FKIP, UniversitasTanjung Pura Jln Prof Dr H Hadari Nawawi, PontianakTanggaraKota Pontianak, Kalimantan Barat 78115

Dr Adriani, MSiFakultas Peternakan, Universitas Jambi Jln JambiMuaro Bulian km 15 Mandalo Darat Jambi

Prof Komang BudaarsaFakultas Peternakan Universditas Udayana JlnSudirman Denpasar Bali

Dr Mien Theodora Rossesthellinda LapianProgram Studi Ilmu Ternak, Laboratorium ProduksiTernak, Fakultas Peternakan Universitas SamratulangiManado, Sulawesi Utara, Fax.0431863186, [email protected].

Dr Andi Mu’nisaJurusan Biologi, FMIPA, Universitas NegeriMakassar, Parang Tambung, Jln Daeng Tata Raya,Makassar 90223

Dr drh IB Swacita MPLab Kesehatan Masyarakat Veteriner FakultasKedokteran Hewan Universitas Udayana, JlnSudirmsn Denpasar Bali

Dr drh Joko Prastowo MSiBagian Parasitologi, Fakultas Kedokteran Hewan,Universitas Gadjah Mada, Jln Fauna No 2, KampusUGM Jogjakarta

Dr JakariaLab Genetika, Fakultas Peternakan, Institut PertanianBogorJln Agathis, Kampus IPB Dramaga Bogor 16680

Anni Nurliani SSi, MScJln Sekumpul Gg Latansa No 5RT 3 RW 1, KelSekumpul, Kec Martapura, Kabupaten BanjarKalimantan Selatan 70614

Dr MachfudzDept Parasitology, Fakultas Kedokteran, UniversitasAirlangga, Jln Prof Dr Moestopo No 47 Surabaya60131


Dr Ni Nengah Dwi Fatmawati SPMK, PhDBagian/SMF Mikrobiologi Klinik, RSUPSanglah,Fakultas Kedokteran, Universitas Udayana

Dr Drh Rahmat Setya AdjiBagian bakteriologiBalai Besar Penelitian VeterinerJln Re Martadinata No 30 Bogor 16114

Dr drh Maxs Urias Ebenhaizar Sanam MScFakultas Kedokteran Hewan, Univesitas Nusa CendanaJln Adi Sucipto, Penfui, Kupang, NTT 85001

Drh Mokhamad Fakhrudin PhdLab Embriologi Fakultas Kedokteran Hewan InstitutPertanian Bogor Jln Agatis Dramaga Bogor 16680




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drh Sri Gustari MPBagian Reproduksi dan KebidananFakultas KedokteranHewan, Universitas Gajah Mada Jln Fauna No 2Kampus UGM Jogjakarta

Drh Yulvian Sani PhDBagian Farmakologi & Toksikologi Balai BesarPenelitian Veteriner Jln Re Martadinata No 30 Bogor16114

Dr Gagak Donny SatriaBagian Farmakologi, Fakultas Kedokteran Hewan,Universitas Gadjah Mada, Kampus UGM, Jln FaunaNo 2 Jogjakarta

Dr drh Andriyanto. MSIBagian Farmakologi & Toksikologi DepartemenAnatomi Fisiologi Farmakologi, Fakultas KedokteranHewan, Institut Pertanian Bogor, Jalan Agatis KampusIPB Darmaga Bogor 16680

Prof Dr drh Aulanni’amJurusan Kimia/Lab Biokimia, Fakultas Matematika danIlmu Pengetahuan Alam, Universitas Brawijaya, JlnVeteran Malang

Prof Dr Drh I Ketut Berate MsiLab Patologi, Fakultas Kedokteran Hewan, UniversitasUdayana, Jln SudirmanDenpasar Bali

Prof Drh Arief Boediono PhDLab Embriologi, Fakultas Kedokteran Hewan InstitutPertanian BogorJln Agatis Dramaga Bogor 16680

Prof drh Ekowati Handharyani PhDLab Patologi, Fakultas Kedokteran Hewan InstitutPertanian Bogor, Jln Agathis. Kampus IPB DramagaBogor 16680

Prof drh Anak Agung Ayu Mirah Adi, MSi, PhDLab Patologi, Fakultas Kedokteran Hewan, UniversitasUdayana, Jln SudirmanDenpasar Bali

Prof drh I Nyoman Mantik Astawa PhDLab Virologi, Fakultas Kedokteran Hewan, UniversitasUdayana, Jln Sudirman Denpasar Bali

Dr Rini WidayantiBagian Biokimia Fakultas Kedokteran HewanUniversitas Gajah Mada Jln Fauna No 2 Kampus UGMJogjakarta 55281

Prof Dr Drh Gusti Ayu Yuniati Kencana, MPLab Virologi Veteriner Fakultas Kedokteran HewanUniversitas Udayana Jalan Sudirman Denpasar Bali

Dr Drh Michael Haryadi WibowoLab Mikrobiologi, Fakultas Kedokteran Hewan,Universitas Gadjah Mada Kampus UGM, Jln FaunaNo 2 Jogjakarta

Prof drh M Lazuardi, MSc, PhDBagian Farmasi, Fakultas Kedokteran HewanUniversitas Airlangga, Kampus-C Unair JlnMulyorejo, Surabaya

Dr Ietje WientarsihLab Farmasi, Dept Klinik Patologi Reproduksi,Fakultas Kedokteran Hewan,Institut Pertanian BogorJln Agathis Kampus IPB Dramaga Bogor 16680


Prof Dr Drh Fachriyan H PasaribuLab Mikrobiologi Medik, Fakultas KedokteranHewan, Institut Pertanian Bogor Jln Agathis KampusIPB Dramaga Bogor 16680


Dr Gagak Donny SatriaBagian Farmakologi, Fakultas Kedokteran Hewan,Universitas Gadjah Mada, Kampus UGM, Jln FaunaNo 2 Jogjakarta

Prof. Dr. Supar, MS, APUKompleks Perumahan-Balitvet Tajur No 47/49 JalanRaya Tajur, Bogor





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RALAT

Bersama ini kami sajikan ralat abstrak artikel atas nama Siti Gusti Ningrum et al; edisiMaret 2016. Abstrak bahasa Indonesia ini adalah pengganti abstrak bahasa Inggris yangditampilkan secara ganda pada penerbitan tersebut. Atas kekeliruan ini redaksi minta maaf.

ABSTRAK

Staphylococcus aureus adalah penyebab utama mastitis pada sapi perah. Pada penelitian ini,sebanyak 104 isolat Staphlylococcus yang berasal dari mastitis subclinik pada sapi dikarakterisasi secarafenotipe dan melacak keberadaan protein-A Staphylococcus (Spa). Resistensi sejumlah bakteri terhadapjuga dilakukan terhadap erythromycin, streptomycin, tetracycline, cefepime, nitrofurantoin, amikacin,chloramphenicol, dan ciprofloxacin. Sekitar 78% dari isolate tersebut bersifat sensitive sedang terhadapnitrofurantoin, sedangkan 89% sangat resisten terhadap cefepime dan ciprofloxacin. Dengan menggunakanberbagai sera mammalia, tujuh isolate terungkap menunjukkan memiliki Spa.

Kata-kata kunci: protein A, Staphylococcus aureus, mastitis subklinik, phenotyping

Kulit Dalem

CETAK LEPAS / REPRINTVol 17, No 3 September 2016

Terakreditasi Dirjen DiktiS.K. No. 81/DIKTI/Kep/2011




Naskah AsliOriginal Article

WYANDA ARNAFIA, SITI GUSTI NINGRUM, ERFIANDINI EKA PUSPITA,DENNY WIDAYA LUKMAN, FACHRIYAN HASMI PASARIBU, I WAYAN TEGUH WIBAWANCross Reaction of Serum in Salmonella enteritidis-Vaccinated Chicken to Some Salmonella enterica Serotypes(REAKSI SILANG SERUM AYAM YANG DIVAKSIN DENGAN SALMONELLA ENTERITIDIS TERHADAP BEBERAPA SEROTIPE SALMONELLA ENTERICA) ............................ 316-321

RISA INDRIANI, NI LUH PUTU INDI DHARMAYANTIPengembangan Vaksin Inaktif TeteloGenotipe VII Isolat Lokal pada Kondisi Laboratorium.(DEVELOPMENT OF TETELO INACTIVATED VACCINEGENOTYPE VII LOCAL ISOLATE IN LABORATORY CONDITION) ................................................... 322-330

ANDIKA BUDI KURNIANTO, GUSTI AYU YUNIATI KENCANA,I NYOMAN MANTIK ASTAWARespons Antibodi Sekunder Terhadap Penyakit Tetelo pada Ayam Petelur PascavaksinasiUlangan dengan Vaksin Tetelo Aktif(NEWCASTLE DISEASESECONDARY ANTIBODY RESPONSEAFTER REVACCINATION IN LAYER WITH THE ACTIVE ND VACCINE) ........................................ 331-336

WAHONO ESTHI PRASETYANINGTYAS, DENY PUTRA ROMADHON, FITRI SUSANA,ITA DJUWITA, KUSDIANTORO MOHAMADBlack Seed (Nigella sativa) Extract Induce in Vitro Proliferationand Differentiation of Rat Pancreatic and Bone Cells(EKSTRAK JINTAN HITAM (Nigela sativa) MENGINDUKSI PROLIFERASIDAN DIFERENSIASI SEL PANKREAS DAN SEL TULANG TIKUS SECARA IN VITRO) ................. 337-346

WORO HASTUTI SATYANTINI, AGUSTONO, ARIMBI,EMY KOESTANTI SABDONINGRUM, MYRNA BUDI, LINA WAFIA ASMIPeningkatan Respons Imun Non Spesifik Ikan Gurame PascapemberianEkstrak Air Panas Mikroalga Spirulina platensis(ENHANCEMENT OF NON-SPECIFIC IMMUNE RESPONSE OF OSPHRONEMUS GOURAMYAFTER GIVING OF HOT WATER EXTRACT SPIRULINA PLATENSIS) ............................................. 347-354

KHAIRUN NISAA, SUKENDA, MUHAMMAD ZAIRIN JUNIOR,ANGELA MARIANA LUSIASTUTI, SRI NURYATIBenih Keturunan Induk Ikan Nila yang Divaksinasi pada Tingkat Kematangan Gonad-2Lebih Tahan Terhadap Infeksi Streptococcus agalactiae(RESISTANCE OF FRY FROM TILAPIA (OREOCHRIMIS NILOTICUS) VACCINATIONBROODSTOCK AT DIFFERENT GONADAL DEVELOPMENTAL STAGESTO STREPTOCOCCUS AGALACTIAE INFECTION) ................................................................................ 355-364

NINING ARINI, MIRNAWATI SUDARWANTO, IDWAN SUDIRMAN, AGUSTIN INDRAWATIPemanfaatan Supernatan Lactobacillus plantarum Sebagai PenghambatPertumbuhan Escherichia coli pada Dangke Susu Sapi(UTILIZATION OF LACTOBACILLUS PLANTARUM SUPERNATANAS AN INHIBITIOR OF ECHERICHIA COLI GROWTH IN COW’S MILK DANGKE .......................... 365-373

KORBINIANUS FERIBERTUS RINCA, TJOKORDA SARI NINDHIA,I WAYAN SUARDANAFaktor-faktor Risiko Penyebaran Escherichia coli O157:H7pada Sapi Bali di Kuta Selatan, Badung, Bali(RISK FACTORS FOR DISSEMINATION OF ESCHERICHIA COLI O157:H7IN BALIN CATTLE IN SOUTH KUTA, BADUNG, BALI) ......................................................................... 374-382

TRI NURHAJATI, KOESNOTO SOEPRANIANONDO,WIDYA PARAMITA LOKAPIRNASARIUji Aktivitas Pertumbuhan Enterobacter cloacae Selulolitik Aerob Rumen-1 IsolatAsal Limbah Cairan Rumen Sapi Peranakan Ongole(GROWTH ACTIVITY ASSAY OF CELLULOLYTIC BACTERIA ENTEROBACTER CLOACAESAR 1 (CELLULOLYTIC AEROB RUMEN 1) ISOLATED FROM ONGOLE CROSSBREEDBOVINE RUMEN FLUID WASTE) ................................................................................................................. 383-388

I GUSTI AYU DEWI RATNAYANTI, NI PUTU SRIWIDYANI, I DEWA AYU INTEN PRIMAYANTI,I GUSTI KAMASAN, NYOMAN ARIJANA, I GUSTI NYOMAN SRI WIRYAWAN,IDA AYU IKA WAHYUNIARI, I WAYAN SUGIRITAMA, I GUSTI NGURAH MAYUNGrowth Hormone Menurunkan Ekspresi Protein p53 dan p21 Sel Endotel Tikus Jantan(GROWTH HORMONE REDUCES P53 AND P21 ENDOTHELIALPROTEIN EXPRESSION IN MALE RAT) .................................................................................................... 389-395

Vol 17, No 3 September 2016






CETAK LEPAS / REPRINT

NOVI SUPRIHATIN, LIGAYA ITA TUMBELAKA, MOHAMAD AGUS SETIADIProfil Progesteron Air Susu dan Tingkat Kebuntingan Sapi Perah PascasinkronisasiEstrus Menggunakan Prostaglandin F

2Alfa atau Progesteron-CIDR

(MILK PROGESTERONE PROFILE AND PREGNANCY RATE ON DAIRY CATTLEAFTER ESTROUS SYNCHRONIZATION WITH PROSTAGLANDIN F

2

ALFA OR PROGESTERONE-CIDR) .............................................................................................................. 396-403

RINI WIDYASTUTI, SONY HERU SUMARSONO, ARIEF BOEDIONO, SITI DARODJAH RASADLow Concentration of Ethylene Glycol Improve Recovery Rate of HumanSpermatozoa After Vitrification(ETILEN GLIKOL KONSENTRASI RENDAH MENINGKATKANRECOVERY RATE SPERMATOZOA MANUSIA PASCAVITRIFIKASI) ................................................. 404-410

YULIANA, SIANNY HERAWATIPhytochemical Content and Protective Effect of Kleinhovia hospital Leaves Extracton Pancreatic Cytotoxicity in Hyperglycemic Rats(KANDUNGAN FITOKIMIA DAN EFEK PROTEKTIF EKSTRAK DAUN PALIASA (KLEINHOVIAHOSPITA Linn) PADA SITOTOKSISITAS PANKREAS TIKUS HIPERGLIKEMIA) ........................... 411-417

ISWINARNO DOSO SAPUTRO, LOBREDIA ZARASADE, REVITA WIDYA PRASANTIHambatan Kolonisasi Methicillin Resistant Staphylococcus Aereus oleh EkstrakKulit Delima pada Luka Bakar Derajat-2 pada Tikus(INHIBITION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS BACTERIACOLONIZATION ON SECOND DEGREE BURN WOUND IN WISTAR RATBY USING POMEGRANATE PEEL EXTRACT) ......................................................................................... 418-423

IWAN HARJONO UTAMA, SRI KAYATI WIDYASTUTI, ANAK AGUNG AYU MIRAH ADI,I GUSTI MADE KRISNA ERAWAN, IDA BAGUS OKA WINAYA, IDA BAGUS KOMANG ARDANA,ACHOIRO WATI RASID, TYAS PANDIEKA YOGAGambaran Sitologi Cairan Peritoneal dan Sinovial Itik Bali(CYTOLOGIC FIGURE OF PERITONEAL AND SYNOVIAL FLUID IN BALI DUCKS) ..................... 424-429

DILLA MAREISTIA FASSAH, LILIS KHOTIJAHPengimbuhan Vitamin-E dalam Ransum Kaya Asam Lemak Tidak JenuhTerhadap Profil Darah Induk Domba Laktasi§

(VITAMIN-E SUPPLEMENTATION ON RICH POLY-UNSATURATEDFATTY ACID RATION TO BLOOD PROFILE OF LACTATION EWES) ............................................... 430-439

HILMA PUTRI FIDYANDINI, MUNTI YUHANA, ANGELA MARIANA LUSIASTUTIPemberian Probiotik Multispesies dalam Media Budi Daya Ikan Lele Dumbo untukMencegah Penyakit Motile Aeromonads Septicemia(ADDITION OF MULTISPECIES PROBIOTICS IN THE CULTURE MEDIUM OF AFRICANCATFISH TO PREVENT THE MOTILE AEROMONADS SEPTICEMIA DISEASE) .......................... 440-448

ANINDYA KURNIAWATI, DENNY WIDAYA LUKMAN, I WAYAN TEGUH WIBAWANResistensi Antibiotik pada Salmonella Isolat Sapi Bakalan Asal Australia yang DiimporMelalui Pelabuhan Tanjung Priok Jakarta(ANTIBIOTIC RESISTANCE OF SALMONELLA ISOLATES FROM AUSTRALIAN IMPORTEDFEEDER CATTLES THROUGH TANJUNG PRIOK PORT JAKARTA) ................................................ 449-456

MUHAMMAD RIZA, MUHAMMAD RIYADHIFertilitas Semen Kerbau Rawa (Bubalus bubalis carabanensis) yang Diencerkandengan Pengencer Nira Aren(FERTILITY OF SWAMP BUFFALO SEMEN (BUBALUS BUBALIS CARABANENSIS)DILUTED WITH SUGAR PALM JUICE EXTENDER) ............................................................................... 457-467

DATIK SETIAWATI, BAMBANG SUKAMTO, HANNY INDRAT WAHYUNIPengimbuhan Enzim Fitase dalam Ransum Ayam Pedaging Meningkatkan PemanfaatanKalsium untuk Pertumbuhan Tulang dan Bobot Badan(ADDING PHYTASE ENZYMES ON BROILER RATION INCREASING CALCIUM UTILIZATIONFOR BONE GROWTH AND BODY WEIGHT GAIN) ................................................................................ 468-476

PEDOMAN BAGI PENULIS ................................................................................................................................... i-ii

411

Phytochemical Content and Protective Effect

of Kleinhovia hospital Leaves Extract

on Pancreatic Cytotoxicity in Hyperglycemic Rats

(KANDUNGAN FITOKIMIA DAN EFEK PROTEKTIF

EKSTRAK DAUN PALIASA (KLEINHOVIA HOSPITA Linn)

PADA SITOTOKSISITAS PANKREAS TIKUS HIPERGLIKEMIA)

Yuliana1, Sianny Herawati2

1Department of Anatomy, 2Department of Clinical Pathology,

Faculty of Medicine, University of Udayana,

Jln. Sudirman, Denpasar, Bali, Indonesia

Phone: 0361 222510; Email: [email protected]

ABSTRACT

Diabetes mellitus is a wide problem nowadays. Many traditional plants are used to overcome thecomplication of this disease. One of them is paliasa (Kleinhovia hospital Linn) leaves. This study aimswere to investigate phytochemical contents and the protective effects of paliasa leaves extract on pancreaticcytotoxicitybased on microscopic lesion such as hemorrhagic score and necrotic appearances, in alloxan-induced hyperglycemic rats. Male Wistar rats (three months old) were divided into four groups consistedofseven rats each. Group I was diabetic control, Group II was diabetic groups, paliasa extract (300mg/kgBW) were given by sonde for a period of 14 days prior to alloxan injection (150 mg/kg intraperitoneal).Group III was diabetic rats given 600 mg/kg BW paliasa extract and group IV was diabetic rats given 900mg for 14 days. At the end of the study, rats were sacrificed. Tissue sample ofpancreaswas then processedfor slide preparation and was stain with hematoxylin eosin.Pancreas hemorrhagic score was divided intofour criteria, focal(score 1), multifocal (score 2), extensive (score 3), and difuse (most severe, score 4).Microscopic examination was done using binocular microscope, at Laboratory ofVeterinaryPathology,Disease Investigation Centre Denpasar, Bali. Data werethenanalyzed by using analysis ofvarians. Study showed that paliasa extract could lower hemorrhagic score on pancreas of diabetic rats,even though it was not significant compared to control group (p 0,205). Phytochemical analysis showedthat paliasa leaves extract contained alkaloid, terpenoid, and flavonoid. Necrotic appearances were variedfrom pycnosis, karyorheksis, karyolysis, and vacuolization. In conclusion, paliasa leave extract may haveprotective effect on pancreas cytotoxicity.

Key words: alloxan; paliasa extract; diabetes; phytochemical; rats

ABSTRAK

Diabetes mellitus merupakan masalah yang sering dijumpai di dunia. Banyak tanaman tradisionaldigunakan untuk mengatasi komplikasi penyakit ini. Salah satu di antaranya adalah daun paliasa(Kleinhovia hospital). Penelitian ini bertujuan untuk mengetahui kandungan fitokimia dan efek proteksiekstrak daun paliasa terhadap sitotoksisitas pancreas (tingkat perdarahan dan gambaran nekrosis)pada tikus Wistar hiperglikemia yang diinduksi aloksan. Penelitian menggunakan rancangan ControlGroup Design, menggunakan 28 ekor tikus berumur tiga bulan. Tikus-tikus percobaan dibagi secara acakmenjadi empat kelompok, yaitu kelompok kontrol, paliasa dosis 300mg/kg berat badan (BB), 600 mg/kgBB, dan 900 mg/kg BB. Setelah diberikan perlakuan selama 14 hari, tikus dikorbankan nyawanya dandilakukan pemeriksaan pankreas dengan pengecatan hematoxylin eosin. Tingkat perdarahan pankreasdibagi menjadi empat skor lokal (skor 1), multifokal (skor 2), ekstensif (skor 3), dan difus (paling berat,diberi skor 4). Pemeriksaan mikroskopis memakai mikroskop cahaya binokuler. Pemeriksaan dilakukandi Laboratorium Patologi Veteriner, Balai Besar Veteriner (BB Vet) Denpasar, di Pegok, Denpasar, Bali.Analisis data dilakukan dengan sidik ragam. Penelitian menunjukkan bahwa ekstrak paliasa dapatmenurunkan tingkat perdarahan pancreas tikus hiperglikemik, namun tidak signifikan (p 0,205). Ujifitokimia dilakukan di Jurusan Farmasi Fakultas MIPA Universitas Udayana. Hasil penelitianmenunjukkan uji fitokimia ekstrak tanaman paliasa mengandung alkaloid, terpenoid, dan flavonoid.Gambaran nekrosis bervariasi dari piknosis, karioreksis, kariolisis, dan vakuolisasi. Ekstrak tanamanpaliasa memiliki efek protektif pada sitotoksisitas pancreas.

Kata-kata kunci: aloksan; ekstrak paliasa; diabetes; fitokimia; tikus

Jurnal Veteriner September 2016 Vol. 17 No. 3 : 411-417pISSN: 1411-8327; eISSN: 2477-5665 DOI: 10.19087/jveteriner.2016.17.3.411Terakreditasi Nasional SK. No. 15/XI/Dirjen Dikti/2011 online pada http://ojs.unud.ac.id/php.index/jvet

412

INTRODUCTION

Diabetes mellitus is a worldwide disease and

is depicted in blood glucose increasing above

normal. World Health Organization predicted

that more than 220 million people in the world

will suffer from diabetes and this number will

be doubled in 2030 (Hamid and Moustafa, 2013).

In Indonesia, it is estimated that 50% of diabetic

patients had not been diagnosed (Muhtadi et al.,

2015).

Oral anti-diabetic and insulin sensitizer can

repair insulin insensitivity, increase insulin

production, and lower blood glucose. However,

those medicines have some side effects, such as

hypoglycemia at higher dosage, low oral

bioavailability, and low permeability when

entering intestine epithelium. This will urge

increasing demand of anti diabetic herbal

products with relatively fewer side effects

(Malekpour et al., 2012). One of traditional

herbal plants that used to treat diabetes is

paliasa. Paliasa (Kleinhovia hospital Linn) is

found at South Sulawesi. Paliasa leaves can be

used as vegetables, supportive medicine for liver

disease, and combat hair flea (Dini, 2009).

Diabetes mellitus induced by alloxan is type-

1 diabetes mellitus, insulin dependent diabetes

mellitus (Viana et al., 2004). It happened due to

autoimun disorder and beta cell pancreas

damage in Langerhans islets. First, beta cell

pancreas decrease in number and volume, then

insulin deficiency will happen permanently

(Waer and Helmy, 2012). Diabetes mellitus

treatment without any side effects is a big

challenge for future. WHO declared that anti-

diabetic herbal medicine need special concerns

(Patil et al., 2011).

Hiperglycemia stimulates reactive oxygen

species from many ways such as oxidative

fosforilation, glucose autooxidation, lipooxy-

genase, and nitrit oxide sintase. Natural anti

oxidant in our body is not sufficient to neutralize

free radicals that can cause tissue damage. Thus,

herbal anti oxidant is needed as an alternative

for the treatment (Hamzah et al., 2012).

In diabetic rats, pancreas cells showed

granular degeneration, pycnosis, karyorrhexis,

and karyolysis (Liu et al., 2014). In histo-

pathology of alloxan induced diabetic rats, Mir

et al. (2013) found changes in exocrine and

pancreatic beta cells. On the islets of Langerhans

cell degranulation occurs, accompanied by the

formation of ghost islets with loss of whole cells.

Bleeding is also found in all parts of the gland.

This study aims were to investigate phyto-

chemical contents and the protective effects of

paliasa leaves extract on pancreatic cytotoxicity

(hemorrhagic score and necrotic appearances) in

alloxan-induced hyperglycemic rats.

RESEARCH METHODS

Plant Material Collection and Preparation

Paliasa leaves were harvested from

Makassar, a city of South Sulawesi Province.

The process of extraction was done as following:

1 kg of dry paliasa leaves, were poured with 15

litres methanol in room temperature (26°C) for

14 hours, then they were filtered with Whatman

paper. Filtrate was dried by vacuum through

rotary evaporation (Arung et al., 2009).

Animals

Three month-old male Wistar rats (180-200

g body weight) bred in Pharmacology Depart-

ment, Faculty of Medicine, University of

Udayana, animal house were used. The rats were

housed in cages with room temperature (26°C).

This experimental protocol was approved by

Ethics Committee of Animal Usage for Research

and Education from Faculty of Veterinary

Medicine, Udayana University (Letter Number:

191/KE-PH-Lit-2/V/2015 on 12 May 2015).

Alloxan was purchased from Pharmacological

Laboratory of Widya Mandala University,

Surabaya, Indonesia.

Experimental Design

A total of 28 rats were randomly divided into

four groups of seven each and induced with

alloxan 120 mg/kg BW intraperitoneal in saline.

All rats were found to be diabetic after 72 h. Rats

with a blood glucose level above 150 mg/ dL were

considered to be diabetic (Agunbiade et al., 2015).

Group I served as the diabetic control; Group II

were given paliasa leaves extract by using sonde

orally with dose 300 mg/kg BW; Group III were

given paliasa leaves extract by using sonde orally

with dose 600 mg/kg BW; Group IV were given

paliasa leaves extract by using sonde orally with

dose 900 mg/kg BW. The curative effect of paliasa

extract was then evaluated for a period of 14 days

after alloxan administration and animals were

sacrificed by using chloroform. Pancreases were

collected immediately for histopathological

examination.

Yuliana, et al Jurnal Veteriner

413

Glycoside. It is done by Liebermann-

Burchard reaction. One mililiter extract was

evaporated; the rest was soluted in 5 mL

anhidrate acetat acid, and then added with 10

drop sulfate acid. The extract has black brown

colour, it meant negative for glycoside.

Flavonoid. One mililiter extract solution

was evaporated, the rest was wetted by aseton,

enhanced by a little of smooth borac acid powder

and oxalic acid, then it was heated carefully. After

that, the rest was mixed with 10 mL eter. By

using UV366

; the extract showed intensive

yellowish fluorescence, and it meant there was

flavonoid. Phytochemical test showed that

paliasa contains alkaloid, terpenoid, and

flavonoid

Statistical Analysis.

All the grouped data were statistically

evaluated with one way analysis of variance

(Anova), P< 0.05 was considered statistically

significant.

RESULTS AND DISCUSSION

Result of treatment effect was shown on

Table 1 (electric microscope, 400x)

The Table 1 showed the mean pancreatic

hemorrhagic score between groups after being

given treatment did not differ significantly (p>

0.05). Necrosis appearances were varies, from

pycnosis, karyolysis, and karyorhexis as shown

in Figure 1.

Alloxan is beta cytotoxin. Alloxan chemically

induces diabetes by destroying beta cells that

produce insulin. A decrease in the release of

insulin lowers insulin usage to neutralize blood

glucose in the body. Increased oxygen free

radicals occur due to high blood glucose levels.

Histopathological Studies

Pancreatic tissues from all groups were

subjected to histopathological studies and

collected in jar contained with approximately

10% buffered formalin solution. Sections of

approximately 5 µm thickness of the pancreas

were made and stained with hematoxylin and

eosin (HE tissue staining) and they were

examined for pancreatic hemorrhagic score.

Stained sections were qualitatively evaluated

using an electric binocular microscope (Olympus

Japan), with magnification 400 times.

Pancreas hemorrhagic score was divided into

four groups, i.e. local (score 1), multifocal (score

2), extensive (score 3), and diffuse (score 4).

Phytochemical Analysis

Phytochemical analysis was done at

Pharmacy Department, of the Faculty of

Mathematics and Natural Science, Udayana

University, in order to investigate the main

content of paliasa extract that has effect in

protecting pancreas.

Atsiri Oil

Extract was added with etanol. Paliasa

leaves extract has no aromatic smell although

it was evaporated until dry.

Alkaloid. Two mililiters extract was

steamed until it get residu. Residu was added

with 5 mL HCl 2N, then it was divided to five

reaction tube. First tube was added with weak

acid that acted as blanko. Second tube was added

with three drops Dragendorrff, third tube was

added with three drops Mayer reaction, fourth

tube was added wagner reaction. The paliasa

extract showed orange sediment in second tube

and white yellowish sediment in third tube. It

showed there was alkaloid.

Sterol and terpenoid. One mililiter

extract was steamed, and then it was added by

0.5 mL chloroform, and added by 0.5 mL anhidrat

acetic acid. Then it was dropped by 12 mL

sulphate acid. The extract showed brownish or

violet ring in the boundary, it revealed there was

terpenoid.

Saponin. Three mililiters paliasa extract

was shaken vertically for 10 seconds, then it

waited for 10 seconds. The extract had no stable

foam, it meant there was no saponin.

Polyphenol. One mililiter extract was

added with iron/ferri (III) chloride 10%, if it

turned to dark blue, blue black or black green,

then it meant there was polyphenol there. The

extract showed negative for polyphenol.

Table 1. Mean pancreatic hemorrhagic score of

diabetic rats after treatment with

paliasa extract (n=7).

Treatment Group Mean pancreatic

hemorrhagic

score ± SD

Control 3,14 ± 0,690

Paliasa 300 2,71 ± 0,756

Paliasa 600 2,57 ± 0,535

Paliasa 900 2,43 ± 0,545

Jurnal Veteriner September 2016 Vol. 17 No. 3 : 407-413

414

angiotensin system locally on the pancreas will

increase pancreatic damage induced by ROS

(Leung, 2007).

Beta cells of diabetic rats showed

vacuolization and a decrease in secretory

granules, fusion of some granules and pycnotic

nuclei (Hamid and Moustafa, 2013). Giving

alloxan causes morphological changes in diabetic

rats in the form of severe damage to the beta

cells of the pancreas, which is characterized by

a decrease in the number of cells, cell damage,

and even cell death (Dahecha et al., 2011).

Necrosis is usually found in the middle of

Langerhans islet because this part is initially

influenced by alloxan (Singh and Gupta, 2007).

Karyolysis, the loss of the cell nucleus, as well

as the presence of remnants (residue) cells that

have vacuole shaped found in research Jelodar

et al. (2005). At the core of beta cells that undergo

karyolisis, also showed debris in the form of the

Auto oxidation produces free radicals and this is

exacerbated by the effects of diabetogenic alloxan

(Hamzah et al., 2012).

Alloxan and its reduction product, dialuric

acid, cause a redox cycle through the formation

of superoxide radicals. Superoxide radicals

experience dismutase releases hydrogen peroxide.

Highly reactive hydroxyl radicals are formed by

Fenton reaction (Rohilla and Adi, 2012).

The effects of reactive oxygen species (ROS)

along with an increase in cytosolic calcium

concentration would lead to massive destruction

of beta cells rapidly (Hamid and Moustafa, 2013).

The severity of tissue damage and the degree of

oxidative stress may depend on an imbalance

between excessive ROS production and

antioxidant defenses in the cells of the pancreas

(Kikumoto et al., 2010). Reactive oxygen species

are involved in various signaling pathways of

angiotensin II. Increased activity of the renin-

Figure 1. Necrosis appearance in pancreas of four study group (HE, 400x). Figure A is pancreas

of hyperglycemic (arrow showed karyorhexis). Figure B is pancreas of hyperglycemic

rats with 300 mg/kg BW paliasa extract (there is pycnosis, karyorhexis, and karyolysis).

Figure C is pancreas of hyperglycemic rats with 600 mg/kg BW paliasa extract (there is

karyorhexis). Figure D is pancreas of hyperglycemic rats with 900 mg/kg BW paliasa

extract (there is pycnosis and vacuola).


415

fragment mass surrounding the cell nucleus

(Boudreau et al., 2006).

Pancreas is more susceptible to oxidative

stress compared to other organs as the cells in

the pancreas have lower anti-oxidant enzymes

(Robertson, 2006). Oxidative stress that induced

by alloxan will cause breakdown of DNA and

activate polysintetase resulting in impaired

synthesis of insulin in the pancreas (Takemoto

et al., 2014). Pancreas functions as a glucose

sensor and insulin-requiring oxygen-rich

environment as well as glucose, so that it can

generate sufficient signal for insulin secretion

and providing adequate insulin to the target

tissues. These special circumstances make

pancreatic beta cells are highly susceptible to

oxidative stress (Lenzen, 2008). Hyperglycemic

conditions lead to an increase in the concen-

tration of H2O

2 and induce catalase activity as a

defense mechanism against free radicals.

However catalase levels may not be sufficient to

prevent the onset of diabetes (Kikumoto et al.,

2010).

Vacuolization is one indication of structural

disruption of membrane permeability, cause

increase in fluid and electrolyte transport into

the cell. Permeability disorders occur due to

reactive oxygen species (Hamid and Moustafa,

2013). Variations of histopathological pancreas

may occur because of differences in the status of

anti-oxidants, as well as the size and maturity

of cells (Mir et al., 2013).

Anti-oxidants contained in the plants may

act to neutralize free radicals. Free radicals are

associated with the development of degenerative

disorders such as diabetes mellitus and

cardiovascular related diseases (Renjith et al.,

2013). A phytochemical test result of paliasa

plant contains alkaloids, terpenoids and

flavonoids. Alkaloids may act to stimulate the

multiplication of existing cells in Langerhans

islets and differentiation into new cells. Alkaloids

maybe also important for the recovery of partial

beta cell (Singh and Gupta, 2007). Alkaloid has

anti-hyperglycemic, anti-inflammatory, and

anti-oxidants effects (Adeneye and Crook, 2015).

Alkaloids lower insulin-mediated glucose disposal

and reduce activities of two enzymes that are

essential for the production of glucose, namely

glycogen phosphorylase and glucose-6-

phosphatase (Ezuruike and Prieto, 2014).

Terpenoids and flavonoids have anti-diabetic

properties. Flavonoids are able to regenerate the

damaged beta cells in alloxan-induced diabetic

rats and act as insulin secretagogous

(Balamurugan et al., 2014). Liu et al. (2007)

found that flavonoids can enhance insulin

sensitivity. Terpenoids and flavonoids may be

able to improve the integrity of the B-

endocrinocyte through increasing the release of

insulin or insulin activity improvement (Snigur

et al., 2008).

Flavonoids can modulate the activity of

glycolytic and gluconeogenic enzymes (Renjith

and Rajamohan, 2012). Flavonoids inhibit the

glucose transporter epithelial cells in the small

intestine and regenerate the damaged beta cells

in the alloxan induced of pancreatic beta cells

(Sharma et al., 2013). The formation of advanced

glycated end products (AGEs) can be prevented,

and there is no atherosclerosis, nephropathy, and

retinopathy (Rahimi et al., 2005).

An efficacy hypoglycemic herb are obtained

through a process of increasing insulin secretion,

inhibit glucose absorption from the intestine,

inhibits glucose production from hepatocytes,

and increase glucose uptake by muscle and fat

(Hui et al., 2009). Giving phytonutrients may

be an effective strategy to overcome the

complications of diabetes by regulating the

enzymes that responsible for metabolizing

glucose (Renjith and Rajamohan, 2012). The

active substance in traditional crops can activate

the regeneration of pancreatic beta cells (Jelodar

et al., 2005). Pancreatic function recovery occurs

through the facilitation of metabolites in insulin-

dependent process (Balamurugan, 2014).

CONCLUSION

The conclusion obtained from the study is:

paliasa plant extracts contain alkaloids,

terpenoids and flavonoids, and paliasa plant

extract given to hyperglycemic rats may not

signifcantly reduce the level of hemorrhagic

pancreas.

SUGGESTION

Further studies are needed to investigate

each phytochemical content of paliasa extract

towards pancreas chemical markers.

ACKNOWLEDGEMENT

The authors are grateful to Udayana Uni-

versity for the financial assistance, with


416

Contract Letter number 1438/UN14.2/PNL/

01.03.00/2015 (Donation of Study Program).

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