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Uji Efek Antibakteri Ekstrak Etanol Tinta Cumi (Loligo duvauceli)

Terhadap Bakteri E.coli Yang Menjadi Penebab !nfeksi "uka #perasi di

$umah %akit Umum Bahteramas%e&araIn Vitro

Nirmalasari,*Tomy Nurtamin,** Sulastrianah **' Program Sarjana Kedokteran Universitas Halu Oleo

** Program Studi Fakultas Kedokteran UHO

ABSTRACT

Hospital a!uired in"etion #H$%& 'as an appeared in"etion on patient in the hospital or other health"aility, 'hih the in"etion did not appear 'hen the patient 'as aepted in the hospital ( Surgial'ound in"etion 'as one o" the kinds o" nosoomial in"etions that ourred in ) hours a"ter surgery($nti+iotis 'as epeted to +e a+le to eliminate +ateria that aused in"etion( Ho'ever, resisten anti+iotisould ause in"etion that 'as di""iult to +e ured( S!uid ink produe anti+akterial ompounds that an +ealternative "or anti+aterial ethanol etrat o" s!uid ink as anti+aterial against E.coli+ateria( This study 'as a

post test eperimental researh 'ith post test only design 'ith treatment o" ethanol etrat o" s!uid ink # loligo

duvauceli) againstE.coli. -oyyline 'as per"ormed as positive ontrol and -.SO #-imethyl Sul"oidase& asthe negative ontrol( The inhi+ition test used various onentration /0000 ppm, 1000 ppm, 100 ppm, /10

ppm, /10 ppm, 21 ppm, and 3/ ppm 'ith 3 repetitions( %4 10used in this study to see ho' +ig the ethanoletrak o" s!uid ink #Loligo duvauceli& is a+le to inhi+it E.coli+ateria( The result o" the researh sho'ed thatthere 'as inhi+ited response againtsE.coli+egan that at the highest onentration to the lo' onentration 'ithinhi+ited 5one diameter 30(00 mm, 6,7/ mm, ,1 mm, /8(00 mm, /2,1 mm, /1, 1 mm, and 3,1 mm o"

positive ontrol( .%4 #.inimum %nhi+itory 4onentration& o" ethanol etrat o" s!uid ink againstE.coli sho'edtur+idity at the onentration 3/ ppm( %410value o" ethanol etrat o" s!uid ink o+tained 'as ,)21 ppm(4onslusions o" this researh s!uid ink ethanol etrat has the a+ility to inhi+itthe gro'th o" +ateria E.coli 'ith.%4 3/ ppm, %410,)21eords* ethanol etrat o" s!uid ink #Loligo duvauceli&, E.coli, anti+aterial ativity, .%4, %410(

PE+,A-U"UA+%n"eksi nosokomial atau yang +iasadise+ut healthcare associated infection#H$%& adalah in"eksi yang didapatkan dan

+erkem+ang selama pasien dira'atdirumah sakit( .enurut French national

Prevalence Survey( 9okasi yang seringterjadi in"eksi nosokomial diantaranyaadalah luka operasi, saluran kemih, salurannapas, kulit dan jaringan, telinga hidungdan tenggorokan, mata, lokasi pemasangan

kateter dan lokasi lainnya #:HO, 00&(.ar'oto #00)&, mengungkapkan

+ah'a di $merika Serikat, Length of Staypasien in"eksi nosokomial +ertam+ah 1;/0hari, angka kematian pasien le+ih tinggimenjadi 2SU pendidikan di %ndonesia,in"eksi nosokomial ukup tinggi yaitu 2@/2< dengan rata@rata 6,8< pada tahun0/0( %n"eksi nosokomial paling umumterjadi adalah in"eksi luka operasi #%9O&(Hasil penelitian terdahulu menunjukan

+ah'a angka kejadian %9O pada rumahsakit di %ndonesia +ervariasi antara @/8, Tairas ?,

:uisan ?( 0/3( -/i efek antiakteri

tinta cumi$cumi (Loligo sp.)

terhadap akteri saluran akar gigi.

.anadoQ Fakultas Kedokteran

Universitas Sam >atulangi .anado(

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>ahmah, .( N( S( T, Utami >(, dan Fitri

N(>(, 0/0( Pemeriksaan esidu

*ntiiotik Pada ati 'erau 6

+kan !ila dengan 0etode 4ifusi

*gar, ?urnal Peternakan ) #/&( 6@37( Sukandar( 006( C+nfeksi Saluran 'emih

Pasien 4e3asa2. -alam Sudoyoso,et.al. Auku $jar %lmu Penyakit-alam( Edisi ( ?akarta Q %nternaPu+lishing(

:orld Health Organi5ation #:HO&, 00(Prevention of ospital$*cquired

+nfections. $ Partial Buide nd