LAPORAN HASIL PENELITIAN HIBAH KOMPETENSI TAHUN ANGGARAN 2011

PENGEMBANGAN PRODUK PANGAN MENGANDUNG POLISAKARIDA GADUNG DAN GEMBILI

UNTUK PENDERITA DIABETES

Prof. Dr. Ir. Harijono, MAppSc. Dr. Teti Estiasih, STP, MP

Wenny Bekti Sunarharum, STP, MFoodSt.

Dibiayai Oleh Direktorat Jendral Pendidikan Tinggi, Kementrian Pendidikan Nasional Sesuai dengan Surat Perjanjian Pelaksanaan Penugasan Penelitian Hibah Kompetensi

Tahun Anggaran 2011 Nomor : 361/SP2H/PL/Dit-Litabmas/IV/2011 tanggal 14 April 2011

UNIVERSITAS BRAWIJAYA

NOVEMBER 2011

BIDANG ILMU: GIZI DAN KESEHATAN

RINGKASAN DAN SUMMARY

RINGKASAN

Bagi penderita diabetes, konsumsi makanan yang tepat sangat diperlukan untuk

mengendalikan kadar gula darah. Produk makanan yang tepat diperlukan sehingga

asupan nutrisi tetap terjaga dengan tetap dapat menjaga kadar gula darah.

Pengembangan produk pangan yang biasa dikonsumsi bagi penderita diabetes perlu

dilakukan dengan menggunakan pendekatan pola konsumsi masyarakat Indonesia.

Penelitian kami sebelumnya menunjukkan bahwa pemberian polisakarida larut air (PLA)

dari gadung dan gembili secara oral pada tikus diabetes dapat menurunkan kadar gula

darah dari hiperglikemia menjadi normoglikemia. Ekstraksi PLA gadung dan gembili

dilakukan secara fermentatif dengan menggunakan ragi tempe. Penelitian Tahun ke-1

berhasil membuat produk pangan berupa bubur dan mie instan mengandung PLA

gadung dan gembili dengan dan atau tanpa kombinasi dengan alginat, yang

menghambat peningkatan kadar gula darah setelah konsumsi. Penghambatan tertinggi

diperoleh dari penambahan PLA 15%. Penambahan PLA gadung dan gembili

dikombinasikan dengan alginat juga menghasilkan karakteristik produk mie dan bubur

instan yang lebih baik.

Pada Tahun ke-2 penelitian ini dilakukan formulasi PLA dari gadung dan gembili

pada produk roti tawar dan biskuit. Hasil formulasi ini akan diperoleh formula mix roti

tawar dan biskuit yang berpotensi HKI. Penelitian ini akan menguji produk roti tawar dan

biskuit mengandung polisakarida gembili/gadung dengan atau tanpa kombinasi dengan

alginat terhadap penurunan kadar gula darah pada kondisi hiperglikemia sehingga juga

berperan sebagai pangan untuk obat (medicinal foods). Kombinasi PLA gadung atau

gembili dengan alginat perlakuan: penambahan alginat, PLA gadung, PLA

gadung+alginat, PLA gembili, PLA gembli+alginate, dan taraf penambahan 10%. Juga

dilakukan control berupa tikus normal yang diberi pakan standar (kontrol normal),

control tikus hiperglikemia diberi pakan standar (kontrol positif), dan roti tawar atau

biskuit tanpa penambahan PLA. Pada akhir masa percobaan, jenis-jenis dan kadar

asam lemak rantai pendek (SCFA, short chain fatty acids) pada caecum tikus akan

dianalisis dan dilakukan meal tolerance test (MTT) untuk mengetahui efek

penghambatan polisakarida terhadap absorpsi glukosa sesaat setelah konsumsi.

Pengujian organoleptik dilakukan dengan uji hedonic skala 1-7.

Produk pangan berupa roti tawar dan biskuit untuk penderita diabetes secara

organoleptik dapat diterima oleh konsumen. Penambahan PLA gadung dan gembili

dikombinasikan atau tanpa kombinasi dengan alginat pada roti tawar dan biskuit dapat

menghambat peningkatan kadar gula darah. Penghambatan yang tajam terjadi pada

roti tawar dan biskuit yang ditambah alginat, diikuti PLA gadung+alginat, PLA

gembili+alginat, PLA gadung, PLA gembili, dan tanpa PLA. Hasil penelitian ini

menunjukkan bahwa adanya PLA gadung/gembili dengan atau tanpa alginat dapat

menghambat penyerapan gula darah dari produk. Produk roti tawar dan biskuit dapat

digunakan oleh penderita diabetes karena tidak menyebabkan peningkatan kadar gula

darah yang tajam.

Pemberian roti tawar dan biskuit mengandung PLA gadung/gembili dengan atau

tanpa alginat dalam jangka panjang dapat menurunkan kadar gula darah pada kondisi 4

hiperglikemia. Urutan penurunan dari yang paling tajam adalah roti tawar dan biskuit

yang ditambah alginat, PLA gadung+alginat, PLA gembili+alginat, PLA gadung, dan

PLA gembili. Tikus kontrol yang diberi pakan standar menunjukan peningkatan kadar

gula darah, demikian pula untuk tikus yang diberi roti tawar dan biskuit tanpa PLA. PLA

gadung lebih efektif dalam menurunkan kadar gula darah dibandingkan PLA gembili.

SCFA yang terbentuk pada digesta caecum untuk perlakuan PLA gadung/gembili dan

alginat lebih rendah dibandingkan tikus kontrol yang diberi pakan standar AIN 93M.

Mekanisme penurunan kadar gula darah pada tikus yang diberi roti tawar dan biskuit

mengandung PLA lebih didominasi oleh penghambatan penyerapan nutrisi dan efek

rasa kenyang. Hal ini perlu dibuktikan lebih lanjut.

Kata kunci: polisakarida, gadung, gembili, diabetes, formula mix

SUMMARY

For patients with diabetes, a proper diet is necessary to control blood sugar

levels. Proper food products are needed so that proper nutrition consumed can still be

maintained while keeping blood sugar levels. Development of commonly-consumed

food products for people with diabetes is needed to be carried out by using a pattern of

consumption of Indonesian society.

Our previous studies showed that administration of water-soluble polysaccharide

(WSP) of Gadung and Gembili orally in Wistar rats with diabetes may reduce blood

sugar levels from hyperglycaemia to normoglycaemia. Fermentative extraction of

Gadung and Gembili WSP was conducted by using a yeast of tempeh. Year 1 study

had successfully develop food products in the form of porridge and noodles containing

WSP of Gadung and Gembili with or without combination with Alginate which inhibit the

increase in blood sugar levels after consumption. The highest inhibition obtained from

the addition of 15% WSP. The addition of Gembili and Gadung WSP combined with

Alginate also produced a better characteristic of products.

In Year 2, study was conducted in formulation of WSP from Gadung and Gembili

on fresh bakery products (white bread) and biscuits. The results of this formulation will

be a formula mix of white bread and biscuits which is potential for a patent application.

This study will analyse the product of white bread and biscuits containing

polysaccharides from Gembili/Gadung with or without combination with Alginate to

decrease blood sugar levels under conditions of hyperglycemia so that it may also

serves as food for drug (medicinal foods). The combination of Gadung or Gembili WSP

with Alginate treatment: the addition of Alginate, Gadung WSP, Gadung+Alginate WSP,

Gembili WSP, Gembili+Alginate WSP, and standard addition level of 10% were applied

in this research.

Treatments applied were addition of Alginate, Gadung WSP, Gadung+Alginate

WSP, Gembili WSP, Gembili+Alginate WSP and the addition level of 10%. Control was

applied as normal Wistar rats with standard feed (normal control), hyperglycemic Wistar

rats with standard feed (possitive control), and Wistar rats fed with white bread or

biscuits without the addition of WSP. At the end of the probationary period, the types

and levels of short chain fatty acids (SCFA, short chain fatty acids) in the rat caecum 5

were analyzed and meal tolerance test (MTT) was performed to determine the inhibitory

effect of polysaccharides on the absorption of glucose shortly after consumption.

Organoleptic test was carried out by hedonic test with a scale of 1-7.

Based on organoleptic test result, food products made for diabetics, i.e. white

bread and biscuits were acceptable. Importantly, the study showed that addition of

Gadung and Gembili WSP combined or without combination with Alginate on white

bread and biscuits may inhibit the increase in blood sugar levels. Sharp inhibition occurs

in white bread and biscuits with Alginate, followed by Gadung+Alginate WSP,

Gembili+Alginate WSP, Gadung WSP, Gembili WSP, and without a PLA. The results of

this study indicated that the presence of PLA Gadung/Gembili with or without Alginate

may inhibit the absorption of blood sugar of the product. White bread products and

biscuits can be consumed by diabetics patients because it does not cause a sharp

increase in blood sugar levels. The provision of white bread and biscuits containing

Gadung/Gembili WSP with or without Alginate in the long term may reduce blood sugar

levels under conditions of hyperglycemia. The sequence of the sharpest decline was

white bread and biscuits with Alginate, Gadung+Alginate WSP, Alginate+Gembili WSP,

Gadung WSP, and Gembili WSP. Control Wistar rats fed with standard feed showed an

increase in blood sugar levels as similar to Wistar rats fed with white bread and biscuits

without a WSP. Gadung WSP had been found to be more effective in lowering blood

sugar levels as compared to Gembili WSP.

SCFA formed in caecum digesta for the treatment of Gadung/Gembili WSP and

Alginate were lower than the control rats fed with standard feed AIN 93M. The

mechanism of lowering blood sugar levels in rats fed with white bread containing WSP

was suggested to be more dominated by the inhibition of nutrients absorption and

satiety. However, this suggestion needs to be further investigated.

Key words: polysaccharides, gadung, gembili, diabetes, mix formula

DAFTAR PUSTAKA

Amici, E.; Tetradis-Meris, G.; Pulido de Torres, C.; & Jousse, F. 2008. Alginate gelation in microfluidic channels. Food Hydrocolloids 22: 97–104.

de Leeuw, J.A., Jongbloed, A.W., and Verstegen, M.W.A. 2004. Dietary fiber stabilizes blood glucose and insulin levels and reduces physical activity in sows (Sus scrofa). J. Nutr. 134: 1481–1486.

de Munter JS, Hu FB, Spiegelman D, Franz M, van Dam RM. 2007. Whole grain, bran, and germ intake and risk of type 2 diabetes: a prospective cohort study and systematic review. PLoS Med. 4:e261.

Edwards, CA.; Blackburn, NA.; Craigen, L.; Davison, P.; Tomlin, J.; Sugden, K.; Johnson, IT.; & Read, NW. 1987. Viscosity of food gums determined in vitro related to their hypoglycemic actions. Am J Clin Nutr 46:72-77.

Fu, Y-C., S. Chen, And Y-J. Lai. 2004. Centrifugation and foam fractionation effect on mucilage recovery from Dioscorea (yam) tuber. Journal of Food Science 69(9): E512-E514.

Harijono; Sunarharum, WB; & Estiasih, T. 2009. Ekstraksi Polisakarida Bioaktif dari Gadung dan Gembili dan Potensinya untuk Terapi Diabetes dan Penurun Kadar Kolesterol Darah. Laporan Penelitian. Universitas Brawijaya, Malang.

Harijono, Estiasih, T., dan Sunarharum, W.B. 2010. Pengembangan Produk Pangan Mengandung Polisakarida Gadung dan Gembili untuk Penderita Diabetes. Laporan Penelitian Hibah Kompetensi Tahun 1, LPPM UB, Malang

Heini AF, Lara-Castro C, Schneider H, Kirk KA, Considine RV, Weinsier RL. 1998. Effect of hydrolyzed guar fiber on fasting and postprandial satiety and satiety hormones: a double-blind, placebo-controlled trial during controlled weight loss. Int J Obes Relat Metab Disord. 22:906–9.

Jenkins D.J., Kendall C.W., Axelsen M., Augustin L.S., Vuksan V.. 2000. Viscous and nonviscous fibres, nonabsorbable and low glycaemic index carbohydrates, blood lipids and coronary heart disease. Curr Opin Lipidol. 11:49–56.

Jing, L. & Yin, L. 2010. Antihyperglycemic activity of polysaccharide from Lycium barbarum. Journal of Medicinal Plants Research 4(1): 023-026.

Kiho T, Kochi M, Usui S, Hirano K, Aizawa K, Inakum T. 2001. Antidiabetic effect of an acidic polysaccharide (TAP) from Tremella aurantia and its degradation product (TAP-H). Biol. Pharm. Bull. 24(12): 1400—1403

Lu, ZX.; Walker, KZ.; Muir, JG.; & O’Dea, K. 2004. Arabinoxylan fibre improves metabolic control in people with Type II diabetes. European Journal of Clinical Nutrition 58: 621–628.

Lunn J, Buttriss JL. 2007. Carbohydrates and dietary fibre. Nutrition Bulletin 32: 21–64 Madar, Z.; Nir, M.; Trostler, N.; & Norenberg, C. 1988. Effects of Cottonseed Dietary Fiber

on metabolic Parameters in Diabetic Rats and Non-Insulin-Dependent Diabetic Humans. J. Nutr. 118: 1143 -1148.

Maki, K.C.; Carson, M.L.; Miller, M.P.; Turowski, M.; Bell, M.; Wilder, D.M.; Rains, T.M.; & Reeves, M.S. 2008. Hydroxypropylmethylcellulose and Methylcellulose Consumption Reduce Postprandial Insulinemia in Overweight and Obese Men and Women. J. Nutr. 138: 292–296.

Mao, X.; Wu, Y.; Wu, K.; Liu, M.; Zhang, J.; Zou, F.; Ou-Yang, J. 2007. Astragalus polysaccharide reduces hepatic endoplasmic reticulum stress and restores glucose

homeostasis in a diabetic KKAy mouse model. Acta Pharmacol Sin 28 (12): 1947–1956.

Marsman K, McBurney M. 1996. Dietary fiber and short-chain fatty acids affect cell proliferation and protein synthesis in isolated rat colonocytes. J. Nutr. 126: 1429-1437

Pi-Sunyer X. 2005. Do glycemic index, glycemic load, and fiber play a role in insulin sensitivity, disposition index, and type 2 diabetes? Diabetes Care 28:2978–9.

Rai, PK.; Singh, SK.; Kesari, AN.; Watal, G. 2007. Glycaemic evaluation of Psidium guajava in rats. Indian Journal of Medical Research 126(3): 224-227.

Robertson MD, Bickerton AS, Dennis AL, Vidal H, Frayn KN. 2005. Insulinsensitizing effects of dietary resistant starch and effects on skeletal muscle and adipose tissue metabolism. Am J Clin Nutr. 82:559–67.

Ruzaidi, A.; Maleyki, A.; Amin, I.; Nawalyah, A.G.; Muhajir, H.; Pauliena, M.B.S. M.J. & Muskinah, M.S. 2008. Hypoglycaemic Properties of Malaysian Cocoa (Theobroma cacao) Polyphenols-Rich Extract. International Food Research Journal 15(3).

Samra RA, Anderson GH. 2007. Insoluble cereal fiber reduces appetite and short-term food intake and glycemic responses to food consumed 75 min later by healthy men. Am J Clin Nutr. 86:972–9.

Schulze MB, Schulz M, Heidemann C, Schienkiewitz A, Hoffmann K, Boeing H. 2007. Fiber and magnesium intake and incidence of type 2 diabetes: a prospective study and meta-analysis. Arch Intern Med. 167:956–65.

Shin KO, Jeon JR, Lee JS, Kim JY, Lee CH, Kim SD, Yu YS, Nam DH. 2006. Lactic acid fermentation of Chinese yam (Dioscorea batatas Decne) flour and its pharmacological effect on gastrointestinal function in rat model. Biotechnology and Bioprocess Engineering 11(3): 240-244

Shobana, S.; Kumari, S.R.U.; Malleshi, N.G. & Ali, S.Z. 2007. Glycemic response of rice, wheat and finger millet based diabetic food formulations in normoglycemic subjects. International Journal of Food Sciences and Nutrition 58(5): 363-372.

Walewijk, A.; Cooper-White, J.J.; Dunstan & D.E. 2008. Adhesion measurements between alginate gel surfaces via texture analysis. Food Hydrocolloids 22: 91–96.

Weickert, M.O. dan A.P.F.H. Pfeiffer. 2008. Metabolic effects of dietary fiber consumption and prevention of diabetes. J. Nutr. 138: 439–442.

Xie, J.T.; Wu, J.A.; Mehendale, S.; Aung, H.H.; Yuan, C.S. 2004. Anti-hyperglycemic effect of the polysaccharides fraction from American ginseng extract in ob/ob mice. Phytomedicine: 182-187.

Al-Habori, Raman A. 1998. Antidiabetic and hypocholesterolaemic effects of fenugreek. Phytotherapy Research 12: 233–242

American Diabetes Association/ADA. 1995-2010. Diabetes Superfoods, http://www.diabetes.org/food-and-fitness/food/what-can-i-eat/diabetes-superfoods.html, diakses 8 April 2010.

Amici, E.; Tetradis-Meris, G.; Pulido de Torres, C.; & Jousse, F. 2008. Alginate gelation in microfluidic channels. Food Hydrocolloids 22: 97–104.

Andari, R.Y. 2009. Pengaruh penggunaan tepung sera dan tepung beras merah terhadap kualitas biskuit (kajian proporsi tepung dan konsentrasi kuning telur). Skripsi Jurusan Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Brawijaya. Malang.

Astawan, M. 2003. Membuat Mie dan Bihun. Penebar Swadaya. Jakarta. Bogasari Baking Centre. 2002. Proses pembuatan roti tawar. Jurusan Teknologi Hasil

Pertanian, Fakultas Teknologi Pertanian, Universitas Brawijaya. Malang.

Braun, L. 2008. Wild yam Dioscorea sp. Complementary Medicine March/April Chan YC, Hsu CK, Wang MF, Liao JW, Su TY. 2006. Beneficial effect of yam on the

amyloid β-protein, monoamine oxidase B and cognitive deficit in mice with accelerated senescence. J Sci Food Agric 86:1517–1525

Chou ST, Chiang BH, Chung YC, Chen PC, Hsu CK. 2006. Effects of storage temperatures on the antioxidative activity and composition of yam. Food Chem. 98: 618–623

de Leeuw, J.A., Jongbloed, A.W., and Verstegen, M.W.A. 2004. Dietary fiber stabilizes blood glucose and insulin levels and reduces physical activity in sows (Sus scrofa). J. Nutr. 134: 1481–1486.

de Munter JS, Hu FB, Spiegelman D, Franz M, van Dam RM. 2007. Whole grain, bran, and germ intake and risk of type 2 diabetes: a prospective cohort study and systematic review. PLoS Med. 4:e261.

Edwards, CA.; Blackburn, NA.; Craigen, L.; Davison, P.; Tomlin, J.; Sugden, K.; Johnson, IT.; & Read, NW. 1987. Viscosity of food gums determined in vitro related to their hypoglycemic actions. Am J Clin Nutr 46:72-77.

Fu, Y-C., S. Chen, And Y-J. Lai. 2004. Centrifugation and foam fractionation effect on mucilage recovery from Dioscorea (yam) tuber. Journal of Food Science 69(9): E512-

E514. Harijono. 2006. Pengembangan proses detoksifikasi umbi-umbian secara mikrobiologis,

Jurusan THP, FTP, Universitas Brawijaya Malang. Harijono. 2008b. Isolasi, purifikasi dan karakterisasi enzim β-glukosidase pada gadung

(dioscorea hispida dennst), Jurusan THP, FTP, Universitas Brawijaya Malang. Harijono; Sari, T.A.; & Martati, E. 2008a. Detoksifikasi Umbi Gadung (Dioscorea hispida

Dennst.) dengan Pemanasan Terbatas dalam Pengolahan Tepung Gadung. Jurnal Teknologi Pertanian 9(2): 75-82.

Harijono; Sunarharum, WB; & Estiasih, T. 2009a. Ekstraksi Polisakarida Bioaktif dari Gadung dan Gembili dan Potensinya untuk Terapi Diabetes dan Penurun Kadar Kolesterol Darah. Laporan Penelitian. Universitas Brawijaya, Malang.

Harijono; Sutrisno, A.; Estiasih, T.; Sunarharum, WB. 2009b. Pengembangan Teknologi Pengolahan Umbi Gadung dan Gembili untuk Pangan dan Bahan Obat dalam rangka Penguatan Petani dan Ketahanan Pangan. Laporan Penelitian Hibah Kompetensi Batch III dibiayai oleh DIKTI. Universitas Brawijaya, Malang.

Harijono, Estiasih, T., dan Sunarharum, W.B. 2010a. Pengembangan Produk Pangan Mengandung Polisakarida Gadung dan Gembili untuk Penderita Diabetes. Laporan Penelitian Hibah Kompetensi Tahun 1, LPPM UB, Malang

Harijono, Estiasih, T., dan Mahatmanto, T. 2010b. Efek hipokolesterolemik polisakarida larut air dari umbi gadung pada kondisi hiperkolesterolemia. Laporan Penelitian FTP UB, Malang.

Heini AF, Lara-Castro C, Schneider H, Kirk KA, Considine RV, Weinsier RL. 1998. Effect of hydrolyzed guar fiber on fasting and postprandial satiety and satiety hormones: a double-blind, placebo-controlled trial during controlled weight loss. Int J Obes Relat Metab Disord. 22:906–9.

Hou WC, Lee MH, Chen HJ, Liang WL, Han CH, Liu YW, Lin YH. 2001. Antioxidant activities of dioscorin, the storage protein of yam (Dioscorea batatas Decne) tuber. J. Agric. Food Chem. 49: 4956-4960

Hsu FH, Lin YH, Lee MH, Lin CL, Hou WC. 2002. Both dioscorin, the tuber storage protein angiotensin converting enzyme inhibitory activities. J. Agric. Food Chem. 50(21): 6109-6113

Jenkins D.J.A., Thorne M.J., Wolever T.M.S., Jenkins A.L., Rao A.V., & Thompson L.U. 1987. The effect of starchprotein interaction in wheat on the glycemic response and rate of in vitro digestion. Am J Clin Nutr 45:946-951.

Jenkins D.J., Kendall C.W., Axelsen M., Augustin L.S., Vuksan V.. 2000. Viscous and nonviscous fibres, nonabsorbable and low glycaemic index carbohydrates, blood lipids and coronary heart disease. Curr Opin Lipidol. 11:49–56.

Jing, L. & Yin, L. 2010. Antihyperglycemic activity of polysaccharide from Lycium barbarum. Journal of Medicinal Plants Research 4(1): 023-026.

Kiho T, Kochi M, Usui S, Hirano K, Aizawa K, Inakum T. 2001. Antidiabetic effect of an acidic polysaccharide (TAP) from Tremella aurantia and its degradation product (TAP-H). Biol. Pharm. Bull. 24(12): 1400—1403

Lee CL, Hung HK, Wang JJ, Pan TM. 2007. Improving the ratio of monacolin K to citrinin production of Monascus purpureus NTU 568 under Dioscorea medium through the mediation of pH value and ethanol addition. J. Agric. Food Chem. (16): 6493-6502

Liu YW, Shang HF, Wang CK, Hsu FL, Hou WC. 2007. Immunomodulatory activity of dioscorin, the storage protein of yam (Dioscorea alata cv. Tainong no.1) tuber. Food and Chemical Toxicology 45: 2312-2318

Liu, J.; Yang, F.; Lu, C.; Yang, Y.; Wen, C.; Hua, K. & Wu, S. 2008. Polysaccharides from Dioscorea batatas Induce Tumor Necrosis Factor-r Secretion via Toll-like Receptor 4-Mediated Protein Kinase Signaling Pathways. J. Agric. Food Chem., 56, 9892–9898.

Liu YM,Lin, KW. 2009, Antioxidative ability, dioscorin stability, and the quality of yam chips from various yam species as affected by processing method, J.Food Sci. 74(2): C118-C125.

Lu, ZX.; Walker, KZ.; Muir, JG.; & O’Dea, K. 2004. Arabinoxylan fibre improves metabolic control in people with Type II diabetes. European Journal of Clinical Nutrition 58: 621–628.

Lunn J, Buttriss JL. 2007. Carbohydrates and dietary fibre. Nutrition Bulletin 32: 21–64 Madar, Z.; Nir, M.; Trostler, N.; & Norenberg, C. 1988. Effects of Cottonseed Dietary Fiber

on metabolic Parameters in Diabetic Rats and Non-Insulin-Dependent Diabetic Humans. J. Nutr. 118: 1143 -1148.

Maki, K.C.; Carson, M.L.; Miller, M.P.; Turowski, M.; Bell, M.; Wilder, D.M.; Rains, T.M.; & Reeves, M.S. 2008. Hydroxypropylmethylcellulose and Methylcellulose Consumption Reduce Postprandial Insulinemia in Overweight and Obese Men and Women. J. Nutr. 138: 292–296.

Mann, J. 2007. Dietary carbohydrate: relationship to cardiovascular disease and disorders of carbohydrate metabolism. European Journal of Clinical Nutrition 61 (Suppl 1): S100–S111.

Mao, X.; Wu, Y.; Wu, K.; Liu, M.; Zhang, J.; Zou, F.; Ou-Yang, J. 2007. Astragalus polysaccharide reduces hepatic endoplasmic reticulum stress and restores glucose homeostasis in a diabetic KKAy mouse model. Acta Pharmacol Sin 28 (12): 1947–1956.

Marsman K, McBurney M. 1996. Dietary fiber and short-chain fatty acids affect cell proliferation and protein synthesis in isolated rat colonocytes. J. Nutr. 126: 1429-1437

Milo, L. November 2009. Defeating Diabetes in The Power of Umami, Food Technology Magazine, vol 63, no 11., Institute of Food Technologist. USA.

Mortensen A, Kulling SE, Schwartz H, Rowland I, Ruefer CE, Rimbach G, Cassidy A, Magee P, Millar J, Hall WL, Birkved FB, Sorensen IK, Sontag G. Analytical and compositional aspects of isoflavones in food and their biological effects. Mol. Nutr. Food Res. 53

Myoda, T., Matsuda, Y., Suzuki, T., Nakagawa, T., Nagai, T. & Nagashima, T. 2006, Identification of soluble proteins and interaction with mannan in mucilage of dioscorea opposita thunb. (chinese yam tuber), Food Sci. Technol. Res., vol. 12, no. 4, pp. 299-302.

Niño J, Jiménez DA, Mosquera OM, Correa YM. 2007. Diosgenin quantification by HPLC in a Dioscorea polygonoides tuber collection from Colombian flora. J. Braz. Chem. Soc. 18(5) 1073-1076

Pi-Sunyer X. 2005. Do glycemic index, glycemic load, and fiber play a role in insulin sensitivity, disposition index, and type 2 diabetes? Diabetes Care 28:2978–9.

Pusat Komunikasi Publik, Sekretariat Jenderal Departemen Kesehatan. 2009. Tahun 2030 prevalensi diabetes di Indonesia mencapai 21,3 juta orang. http://www.depkes.go.id/index.php/berita/press-release/414-tahun-2030-preva-lensi-diabetes-melitus-di-indonesia-mencapai-213-juta-orang.html. diakses 5 April 2010.

Rai, PK.; Singh, SK.; Kesari, AN.; Watal, G. 2007. Glycaemic evaluation of Psidium guajava in rats. Indian Journal of Medical Research 126(3): 224-227.

Robertson MD, Bickerton AS, Dennis AL, Vidal H, Frayn KN. 2005. Insulinsensitizing effects of dietary resistant starch and effects on skeletal muscle and adipose tissue metabolism. Am J Clin Nutr. 82:559–67.

Ruzaidi, A.; Maleyki, A.; Amin, I.; Nawalyah, A.G.; Muhajir, H.; Pauliena, M.B.S. M.J. & Muskinah, M.S. 2008. Hypoglycaemic Properties of Malaysian Cocoa (Theobroma cacao) Polyphenols-Rich Extract. International Food Research Journal 15(3).

Samra RA, Anderson GH. 2007. Insoluble cereal fiber reduces appetite and short-term food intake and glycemic responses to food consumed 75 min later by healthy men. Am J Clin Nutr. 86:972–9.

Schulze MB, Schulz M, Heidemann C, Schienkiewitz A, Hoffmann K, Boeing H. 2007. Fiber and magnesium intake and incidence of type 2 diabetes: a prospective study and meta-analysis. Arch Intern Med. 167:956–65.

Shajeela, P.S., V. R. Mohan, L. Louis Jesudas and P. Tresina Soris. 2011. Nutritional and antinutritional evaluation of wild yam (Dioscorea spp.). Tropical and Subtropical. Agroecosystems 14: 723-730

Shin KO, Jeon JR, Lee JS, Kim JY, Lee CH, Kim SD, Yu YS, Nam DH. 2006. Lactic acid fermentation of Chinese yam (Dioscorea batatas Decne) flour and its pharmacological effect on gastrointestinal function in rat model. Biotechnology and Bioprocess Engineering 11(3): 240-244

Shobana, S.; Kumari, S.R.U.; Malleshi, N.G. & Ali, S.Z. 2007. Glycemic response of rice, wheat and finger millet based diabetic food formulations in normoglycemic subjects. International Journal of Food Sciences and Nutrition 58(5): 363-372.

Sulistyono, E. dan J. Marpaung. 2004. Studi karakter umbi dan kandungan nutrisi umbi Dioscorea spp. Catatan Penelitian. Bul. Agron. 32(2): 39-42.

Syafi’i, I.; Harijono; & Martati, E. 2009. Detoksifikasi Umbi Gadung (Dioscorea hispida Dennst) dengan Pemanasan dan Pengasaman pada Pembuatan Tepung. Jurnal Teknologi Pertanian 10(1): 62-82.

Torsdottir, I.; Alpsten, M.; Holm, G.; Sandberg, F.A.; & Ta-Lli, J. 1991. A Small Dose of Soluble Alginate-Fiber Affects Postprandial Glycemia and Gastric Emptying In Humans With Diabetes. J. Nutr. 121: 795-799.

Walewijk, A.; Cooper-White, J.J.; Dunstan & D.E. 2008. Adhesion measurements between alginate gel surfaces via texture analysis. Food Hydrocolloids 22: 91–96.

Weickert, M.O. dan A.P.F.H. Pfeiffer. 2008. Metabolic effects of dietary fiber consumption and prevention of diabetes. J. Nutr. 138: 439–442.

Wolever, T.M.S.; Gibbs, A.L.; Mehling, C.; Chiasson, J.; Connelly, P.W.; Josse, R.G.; Leiter, L.A.; Maheux, P.; Rabasa-Lhoret, R.; Rodger, N.W.; & Ryan, E.A. 2008. The Canadian Trial of Carbohydrates in Diabetes (CCD), a 1-y controlled trial of low-glycemic-index dietary carbohydrate in type 2 diabetes: no effect on glycated hemoglobin but reduction in C-reactive protein. Am J Clin Nutr 87:114 –25.

Xie, J.T.; Wu, J.A.; Mehendale, S.; Aung, H.H.; Yuan, C.S. 2004. Anti-hyperglycemic effect of the polysaccharides fraction from American ginseng extract in ob/ob mice. Phytomedicine: 182-187.

Yang DJ, Lin JT. 2008. Effects of different storage conditions on steroidal saponins in yam (Dioscorea pseudojaponica Yamamoto) tubers. Food Chem. 110: 670–677.

Zhao, G.; Kan, J.; Li, Z. & Chen, Z. 2005. Structural features and immunological activity of a

polysaccharide from Dioscorea opposita Thunb roots. Carbohydrate Polymers

61:125–131.