Isolasi Dan Identifikasi Escherichia Coli
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ISSN : 1411 - 8327 Isolasi dan Identifikasi Escherichia coli 0157:H7 pada Daging Sapi di Kabupaten Badung Provinsi Bali (ISOLATION AND IDENTIFICATION Escherichia coli 0157:H7 ON BEEFAT BmUNG REGENCY PROVINCE OF BALIl I WAYAN SUARDANA', BAMBANG SUMIARTO~ DAN DENNY WIDAYA LUKMAN~ l1 Laboratoriurn Kesehatan Masyarakat Veteriner FKH-UNUD, J1. PB.Sudirman Denpasar, Denpasar- Bali. Tlp.(0361) 223791. E-mail : [email protected] Laboratorium Kesehatan Masyarakat Veteriner FKH-UGM, . J1. Olah Raga, Karang Malang, Yogyakarta.Tlp. (0274) 563083 Laboratorium Kesehatan Masyarakat Veteriner FKH-IPB, J1. athis, Darmaga Bogor. Tlp. (0251) 625588, 629470(285). E-mail : dennylukman 0 hotmail.com ABSTRAK Keamanan bahan pangan merupakan masalah yang amat penting bagi konsumen dan industri pangan. Cemaran bakteri Escherichia coli dan Coliform indikator sanitasi dalam proses pengolahan bahan pangan. Pelacakan bakteri telah diiakukan secara rutin, temas.uk yang bersifat zoonosis seperti men hasilkan toksin ang dikenal dengan Shiga toxin. Toksin ini dapat menimbulkan diare berdarah, colitis fa enwrhagi dan Lmolitic uremic syndronte i HUS pada manusia. Dalam penelitian hi dipelajari hubungan antara tingkat cernaran dan insidensi Colifornz, E. coli, E. coli 0157 dan E.coli 0157:H7 pada dagng sapi. Bakteri pertarna ditumbuhkan pada media EMBA, selanjutnya dipupuk pada media SMAC dm diakhiri dengan uji aglutinasi lateks untuk memastikan keberadaan bakteri E.coii 0157 dan uji antiserum H7 untuk memastikan isolat yang diisolasi merupakan isolat E.coli 0157:H7, Hasil isolasi dan identiwasi terhadap 89 sampel da 'ng sapi diperoleh hasil rata-rata tingkat cemaran Coliform dan E.coli sebesar 93,Ol 2,64 x 10.' ef$ dan 47,82 + 32,34 n 10' cfulg. Pengujian dengan Spearman's rho rnenunjukkan bahwa tingkat cemaran Colifor-m rnenunjukkan adanya korelasi yang lemah (P>0.05) terhadap munculnya cemaran E.coli, E.coli 0157 maupun terhadap E.coli 0157 : H7 dan barn terlihat adanya korelasi yang sangat kuat (P<0.01) bilamana telah berhasil ditemukan bakteri E,coli dengan kemunculan E.coli 0157 maupun E. coli 01 5 7:H7. Pengujian dengan uji Wilcoxon menunjukkan tingkat cemaran bakteri coliform sangat nyata (P<0.01) lebih besar jika dibandingkan dengan tingkat cemaran Coliform, E.coli, E.coli 0157 dan E-coli 0 157337. Kata-kata kunci : Coliforrn, E.uoli, E, coli 0157:H7, daging sapi ABSTRACT The food safety is an important for both consumers and industries. Coliform and Escherichiu coli have been used as sanitary indicator of food processing industries. The detmtion of foodborne pathogens, including those capable of infecting human such as Escherichia coli 0157:H7 have also been conducted. The bacteria produce toxin konown as Shiga toxin which can cause bloody diarrhea to more serious clinical conditions such as hemorrhagic col~tis and hemolytic uremic syndrome. In the this study the correlation of contamination levels with incidence of Coliform, E.coli, E.coli 01 57 and 0157:H7 in beef were examined. The bacteria were firstly cultured in Eosin Methylene Blue Agar (EMBA) medium, then in Sorbitol MacConkey Agar (SMAC) medium and finally tested by Latex agglutination test to confirm the presence of E.coli 0157. A complete assay was carried by H7 antiserum for the presence of E.coli 0157:H7. From 89 beef samples examined, it was shown that the average of Coliform and E.coli contaminations were 93,01 + 2,64 x 10kfdg and 47,82 k 32,34 x lO'Wcfg repectively. Analysis by Spearman's rho test indicated that there was not si ificant (P>0.05) correlation betwsen Coliform contamination levels and the presence of Kcoli, E.co r i 0157 and 0157:H7. On the other hand, E.coli contamination levels correlated significantly (P<O.Ol) with the presence of E.di 0 157 and E.coli O157:H7. Wilcoxon test indicated the percentage of Colifora was significantly higher (P<0.01) than the percentage E.coli, E.coli 0157 and E.coli 01 57 : H7 contamination. Key words : Coliform, E.coli, E. coli 0157:H7, beef. Jurnal Veteriner, Maret 2007 16
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Transcript of Isolasi Dan Identifikasi Escherichia Coli
ISSN : 1411 - 8327
Isolasi dan Identifikasi Escherichia coli 0157:H7 pada Daging Sapi di Kabupaten Badung Provinsi Bali
(ISOLATION AND IDENTIFICATION Escherichia coli 0157:H7 ON BEEFAT B m U N G REGENCY PROVINCE OF BALIl
I WAYAN SUARDANA', BAMBANG SUMIARTO~ DAN DENNY WIDAYA LUKMAN~
l 1 Laboratoriurn Kesehatan Masyarakat Veteriner FKH-UNUD, J1. PB.Sudirman Denpasar, Denpasar- Bali. Tlp.(0361) 223791. E-mail : [email protected]
Laboratorium Kesehatan Masyarakat Veteriner FKH-UGM, .
J1. Olah Raga, Karang Malang, Yogyakarta.Tlp. (0274) 563083 Laboratorium Kesehatan Masyarakat Veteriner FKH-IPB, J1. athis, Darmaga
Bogor. Tlp. (0251) 625588, 629470(285). E-mail : dennylukman 0 hotmail.com
ABSTRAK
Keamanan bahan pangan merupakan masalah yang amat penting bagi konsumen dan industri pangan. Cemaran bakteri Escherichia coli dan Coliform indikator sanitasi dalam proses pengolahan bahan pangan. Pelacakan bakteri telah diiakukan secara rutin, temas.uk yang bersifat zoonosis seperti men hasilkan toksin
ang dikenal dengan Shiga toxin. Toksin ini dapat menimbulkan diare berdarah, colitis fa enwrhagi dan Lmolitic uremic syndronte i HUS pada manusia. Dalam penelitian h i dipelajari hubungan antara tingkat cernaran dan insidensi Colifornz, E. coli, E. coli 0157 dan E.coli 0157:H7 pada dagng sapi. Bakteri pertarna ditumbuhkan pada media EMBA, selanjutnya dipupuk pada media SMAC dm diakhiri dengan uji aglutinasi lateks untuk memastikan keberadaan bakteri E.coii 0157 dan uji antiserum H7 untuk memastikan isolat yang diisolasi merupakan isolat E.coli 0157:H7, Hasil isolasi dan identiwasi terhadap 89 sampel da 'ng sapi diperoleh hasil rata-rata tingkat cemaran Coliform dan E.coli sebesar 93,Ol 2,64 x 10.' ef$ dan 47,82 + 32,34 n 10' cfulg. Pengujian dengan Spearman's rho rnenunjukkan bahwa tingkat cemaran Colifor-m rnenunjukkan adanya korelasi yang lemah (P>0.05) terhadap munculnya cemaran E.coli, E.coli 0157 maupun terhadap E.coli 0157 : H7 dan barn terlihat adanya korelasi yang sangat kuat (P<0.01) bilamana telah berhasil ditemukan bakteri E,coli dengan kemunculan E.coli 0157 maupun E. coli 0 1 5 7:H7. Pengujian dengan uji Wilcoxon menunjukkan tingkat cemaran bakteri coliform sangat nyata (P<0.01) lebih besar jika dibandingkan dengan tingkat cemaran Coliform, E.coli, E.coli 0 1 5 7 dan E-coli 0 157337.
Kata-kata kunci : Coliforrn, E.uoli, E , coli 0157:H7, daging sapi
ABSTRACT
The food safety is an important for both consumers and industries. Coliform and Escherichiu coli have been used as sanitary indicator of food processing industries. The detmtion of foodborne pathogens, including those capable of infecting human such as Escherichia coli 0157:H7 have also been conducted. The bacteria produce toxin konown as Shiga toxin which can cause bloody diarrhea to more serious clinical conditions such as hemorrhagic col~tis and hemolytic uremic syndrome. In the this study the correlation of contamination levels with incidence of Coliform, E.coli, E.coli 01 57 and 0157:H7 in beef were examined. The bacteria were firstly cultured in Eosin Methylene Blue Agar (EMBA) medium, then in Sorbitol MacConkey Agar (SMAC) medium and finally tested by Latex agglutination test to confirm the presence of E.coli 0157. A complete assay was carried by H7 antiserum for the presence of E.coli 0157:H7. From 89 beef samples examined, it was shown that the average of Coliform and E.coli contaminations were 93,01 + 2,64 x 10kfdg and 47,82 k 32,34 x lO'Wcfg repectively. Analysis by Spearman's rho test indicated that there was not si ificant (P>0.05) correlation betwsen Coliform contamination levels and the presence of Kcoli, E.co r i 0157 and 0157:H7. On the other hand, E.coli contamination levels correlated significantly (P<O.Ol) with the presence of E . d i 0 1 57 and E.coli O157:H7. Wilcoxon test indicated the percentage of Colifora was significantly higher (P<0.01) than the percentage E.coli, E.coli 0157 and E.coli 0 1 57 : H7 contamination.
Key words : Coliform, E.coli, E. coli 0157:H7, beef.
Jurnal Veteriner, Maret 2007 16
