DETEKSI DAN KARAKTERISASI TURNIP MOSAIC VIRUS PENYEBAB PENYAKIT MOSAIK PADA TANAMAN CAISIN (Brassica campestris L. spp. chinensis)

Oleh AdministratorKamis, 11 Oktober 2012 12:21 - Terakhir Diupdate Senin, 15 Oktober 2012 14:05

DETEKSI DAN KARAKTERISASI TURNIP MOSAIC VIRUS PENYEBAB PENYAKITMOSAIK PADA TANAMAN CAISIN

(Brassica campestris L. spp. chinensis)1)

(Detection and Characterization of a Turnip Mosaic Virus Causing Mosaic Disease onCaisin (Brassica campestrisL. spp. chinensis)1)

Firdaus, SP., M.Si

Balai Pengkajian Teknologi Pertanian Aceh

E-mail : firdausjamal30@yahoo.com

ABSTRACT

A mosaic disease is becoming very common on caisin (Brassica campestris L. spp. chinensis)in Bogor, West Java.  Study to identify the causal agent was conducted in Cikabayan screenhouse and Virology Laboratory of Department of Plant Pests and diseases from June 2003 to

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DETEKSI DAN KARAKTERISASI TURNIP MOSAIC VIRUS PENYEBAB PENYAKIT MOSAIK PADA TANAMAN CAISIN (Brassica campestris L. spp. chinensis)

Oleh AdministratorKamis, 11 Oktober 2012 12:21 - Terakhir Diupdate Senin, 15 Oktober 2012 14:05

August 2004. A virus was isolated from caisin plants showing  mosaic symptoms and furtherinvestigation to identity and characterize of the virus was conducted involving several methods,i.e. I-ELISA, RT-PCR, EM, Host range, and transmission studies. Plant samples fromCinangneng, Bogor reacted positively with TuMV polyclonal antisera.  RT-PCR using a specificpair of primer for TuMV was successfully amplified a 800 bp DNA fragment from plant samples.Electron microscopy of leaf dips from plant samples revealed flexuous particle of 11-12 nm wideand 700-780 nm length. The host range of the virus includes plant species belongs to the familyBrassicaceae, Chenopodiaceae, and Solanaceae in which the virus induces necrotic locallesion, and mosaic symptoms. Two aphid species, Myzus persicae and Aphis craccivora, was able to transmit the virus. Based on the above characters, the virus was identified asTuMV.

Key words : Turnip mosaic virus, I-ELISA, RT-PCR, Brassicaceae

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