DAFTAR ISI SURAT PERSETUJUAN irepository.unissula.ac.id/5211/6/daftar isi_1.pdfvi daftar isi surat...
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vi DAFTAR ISI SURAT PERSETUJUAN ....................................................................................... i SURAT PERNYATAAN ................................................................................................... ii RIWAYAT HIDUP ........................................................................................................... iii KATA PENGANTAR ....................................................................................................... iv DAFTAR ISI...................................................................................................................... vi DAFTAR SINGKATAN ................................................................................................... ix DAFTAR TABEL............................................................................................................... x DAFTAR GAMBAR ......................................................................................................... xi DAFTAR LAMPIRAN..................................................................................................... xii ABSTRAK ....................................................................................................................... xiv ABSTRACT...................................................................................................................... xv BAB I PENDAHULUAN ................................................................................................... 1 1.1. Latar belakang..................................................................................................... 1 I.2. Rumusan masalah ..................................................................................................... 3 I.3.Tujuan penelitian ....................................................................................................... 3 I.3.1. Tujuan umum..................................................................................................... 3 I.3.2. Tujuan khusus .................................................................................................... 3 I.4. Manfaat penelitian .................................................................................................... 4 1.4.1 Manfaat Akademik............................................................................................. 4 1.4.2 Manfaat Praktis .................................................................................................. 4 I.5. Originalitas Penelitian .............................................................................................. 4 BAB II TINJAUAN PUSTAKA ........................................................................................ 6 2.1. Penyembuhan Luka ............................................................................................. 6 2.1.1. Patogenesis Luka Bakar .................................................................................... 9 2.1.2 Efek Luka Bakar .............................................................................................. 10
Transcript of DAFTAR ISI SURAT PERSETUJUAN irepository.unissula.ac.id/5211/6/daftar isi_1.pdfvi daftar isi surat...
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DAFTAR ISI
SURAT PERSETUJUAN ....................................................................................... i
SURAT PERNYATAAN ................................................................................................... ii
RIWAYAT HIDUP ........................................................................................................... iii
KATA PENGANTAR ....................................................................................................... iv
DAFTAR ISI...................................................................................................................... vi
DAFTAR SINGKATAN ................................................................................................... ix
DAFTAR TABEL............................................................................................................... x
DAFTAR GAMBAR......................................................................................................... xi
DAFTAR LAMPIRAN..................................................................................................... xii
ABSTRAK....................................................................................................................... xiv
ABSTRACT...................................................................................................................... xv
BAB I PENDAHULUAN................................................................................................... 1
1.1. Latar belakang..................................................................................................... 1
I.2. Rumusan masalah ..................................................................................................... 3
I.3.Tujuan penelitian ....................................................................................................... 3
I.3.1. Tujuan umum..................................................................................................... 3
I.3.2. Tujuan khusus.................................................................................................... 3
I.4. Manfaat penelitian .................................................................................................... 4
1.4.1 Manfaat Akademik............................................................................................. 4
1.4.2 Manfaat Praktis .................................................................................................. 4
I.5. Originalitas Penelitian .............................................................................................. 4
BAB II TINJAUAN PUSTAKA ........................................................................................ 6
2.1. Penyembuhan Luka............................................................................................. 6
2.1.1. Patogenesis Luka Bakar .................................................................................... 9
2.1.2 Efek Luka Bakar .............................................................................................. 10
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2.1.3. Klasifikasi Luka Bakar.................................................................................... 10
2.2. Kadar PDGF...................................................................................................... 10
2.3. Prosentase Kolagen........................................................................................... 11
2.3.1. Sintesis kolagen .............................................................................................. 12
2.3.2. Peran kolagen dalam proses penyembuhan luka............................................. 12
2.4. Mesencymal Stem Cell ........................................................................................... 13
2.5. Pengaruh mesenchymal stem cell terhadap peningkatan kadar PDGF danprosentase kolagen pada penyembuhan luka bakar ...................................................... 15
2.6. Faktor perancu/ pengganggu dalam proses penyembuhan luka............................. 16
BAB III KERANGKA TEORI, KERANGKA KONSEP, HIPOTESIS .......................... 17
3.1. Kerangka Teori ...................................................................................................... 17
3.2.Kerangka Konsep.................................................................................................... 18
3.3. Hipotesis ................................................................................................................ 18
BAB IV METODE PENELITIAN ................................................................................... 19
4.1.Jenis Penelitian dan Rancangan Penelitian ............................................................. 19
4.2. Subyek dan Sampel Penelitian............................................................................... 19
4.3. Variabel Penelitian................................................................................................. 19
4.4. Definisi Operasional Penelitian ............................................................................. 20
4.4.1.Mesencymal stem Cell...................................................................................... 20
4.4.2.Kadar PDGF..................................................................................................... 20
4.4.3.Prosentase Kolagen ......................................................................................... 20
4.5. Bahan atau Metode Penelitian................................................................................ 21
4.6. Alat yang digunakan: ............................................................................................. 21
4.6.1. Alat Kultur: ..................................................................................................... 21
4.6.2. Alat Kultur Vessel: .......................................................................................... 22
4.6.3. Testing Instrumen:........................................................................................... 22
4.6.4.Incubation and Working Culture Area............................................................. 22
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4.6.5. Alat lain:.......................................................................................................... 22
4.7. Prosedur Penelitian ................................................................................................ 23
4.7.1. Teknik Isolasi Mesecymal Stem Cell dari Umbilical Cord ............................. 23
4.7.2. Kultur sel (perbanyak) .............................................................................. 24
Prosedur: .................................................................................................................. 24
4.7.3. Hitung jumlah sel ............................................................................................ 26
4.7.4. Diferensiasi menjadi sel spesifik in vitro ........................................................ 27
4.7.5. Pembuatan luka bakar mencit ......................................................................... 27
4.7.6. Perlakuan (pemberian stem sel pada luka bakar). ........................................... 28
4.7.7. Pembuatan preparat:.................................................................................. 29
4.7.8 Analisis kadar PDGF dengan Elisa.................................................................. 30
4.8. Metode Analisis Data............................................................................................. 32
4.9. Jadwal penelitian.................................................................................................... 32
4.10.Alur Penelitian ...................................................................................................... 33
BAB V HASIL DAN PEMBAHASAN............................................................................ 34
5.1 Hasil Penelitian ................................................................................................. 34
5.2. Pembahasan............................................................................................................ 40
BAB VI KESIMPULAN DAN SARAN .......................................................................... 45
6.1 Kesimpulan ............................................................................................................. 45
6.2. Saran ...................................................................................................................... 46
DAFTAR PUSTAKA ....................................................................................................... 47
LAMPIRAN...................................................................................................................... 50
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DAFTAR SINGKATAN
VEGF : Vascular Endothelial Growth Factor
PDGF : Platelet Derived Growth Factor
IL-1 : Interleukin-1
IL-6 : Interleukin-6
TNF-α : Tumor Necrosis Factor α
NK : Natural Killer
TGF-β : Tumor Growth Factor β
MMPS :Matrix Metalloproteinase Syntesis
CD : Cluster of Differentiation
PMN : Polimor fonuklear
FGF : Fibroblast Growth Factor
ECM : Extra celluler Matrix
EGF : Extra celluler Growth Factor
FGF : Fibroblast Growth Factor
MSCs : Mesenchymal Stem Cells
EPCs : Endogenus Progenitor Cells
mRNA: messenger Ribonukleat Acid
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DAFTAR TABEL
Halaman
Tabel1.5.Originalitaspenelitian….………………………………………………6
Tabel 5.1.1.Rerata Kadar PDGF hari ke 2 danke 7 serta kepadatan Kolagen antara
Kontrol, P1, P2 dan P3………………………....………………..42
Tabel 5.1.2.UjiPost Hoc PDGF harike 2………………………..……...………43
Tabel 5.1.3.UjiPost Hoc PDGF harike 7………………………..…….………..44
Tabel 5.1.5 Uji Post HocKolagen…………………..……………………....….46
Tabel 5.1.7.Uji Korelasi Pearson antara kadar PDGF dan prosentase
kolagen……………………………………………………………..48
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DAFTAR GAMBAR
Halaman
Gambar 2.1.a.Modifikasi Wound Healing………...………...…………….......…. 8
Gambar 2.1.b.Fase Penyembuhan Luka………………………………….............11
Gambar 2.2.Serat kolagen…………………….…...……...………………....…...14
Gambar 2.4.Mesenchymal stem cell……..…..……………………………......….18
Gambar 4.7.3.Kotak Hemocytometer…..………… ………………………....….32
Gambar 5.1.2.Grafikkadar PDGF hari ke-2 antara kontrol, P1, P2 dan P3...........44
Gambar 5.1.3. Grafik kadar PDGF hari ke-7 antara kontrol, P1, P2 dan P3…....45
Gambar 5.1.4.Grafik rata-rata PDGF hari ke-2 dan ke-7……………..………….45
Gambar 5.1.5.Grafik kepadatan kolagen……………………..………………......47
Gambar 5.1.6.Gambaran mikroskopis luka bakar kulit mencit hari ke 10
kelompok P3…………………………………………………….47
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DAFTAR LAMPIRAN
Halaman
Lampiran 1. Hasil analisis berat badan mencit…………………………………..58
Lampiran 2.Hasil Uji Analisis Deskriptif Rata-Rata Kadar PDGF Hari ke-2 … 59
Lampiran 3.Hasil Uji Analisis Normalitas Saphiro-wilk Kadar PDGF Hari ke-2.61
Lampiran 4.Hasil Uji Homogenitas Kadar PDGF Hari ke-2…..………….…….61
Lampiran 5. Hasil Uji One Way Anova dan Post Hoc Test LSD kadar PDGF Hari
ke-2……………………………………..………………………….61
Lampiran 6. Hasil Uji Analisis Deskriptif Rata-Rata Kadar PDGF Hari ke-
7………………………………………………………………….…62
Lampiran 7. Hasil Uji Analisis Normalitas Saphiro-wilk Kadar PDGF Hari ke-
7…………………………………………………………...………..64
Lampiran 8. Hasil Uji Homogenitas Kadar PDGF Hari ke-7…….…….……….64
Lampiran 9HasilUjiOne Way Anova dan Post Hoc Test LSD kadar PDGF Hari
ke-7…………………………………………………………….......65
Lampiran 10.Hasil Uji Analisis Normalitas dan Homogenitas prosentase
Kolagen……………………………………………...……………66
Lampiran 11. Hasil Uji One Way Anova dan Post Hoc Test LSD kepadatan
Kolagen………………………………………………..……….....66
Lampiran 12. Hasil uji beda PDGF hari ke-2 dan ke-7 dengan uji parametric
paired t-test pada tiap kelompok………………………………….67
Lampiran 13. Hasil Uji Korelasi antara Kepadatan Kolagen dengan PDG……...71
Lampiran 14. Proses Isolasi Mesenchymal Stem Cell………………………...……..72
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Lampiran 15. Foto penelitian dari perlakuan mencit luka bakar sampai
pengambilan jaringan untuk pemeriksaan histologi………..……73
Lampiran 16. Ethical Clearance……………………….…………………………74
Lampiran 17.Surat Keterangan Penelitian…………………………………….....75
Lampiran 18. Data berat badan mencit…………………………….…….………79
