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KARYA TULIS AKHIR
PENGARUH EKSTRAK BIJI KAKAO (THEOBROMA CACAO L.) TERHADAP KUALITAS SPERMATOZOA TIKUS PUTIH (RATTUS
NORVEGICUS) JANTAN YANG DIINDUKSI ALLOXAN SEBAGAI AGEN HIPERGLIKEMIK
Oleh:
Pamela Andria Putri Kusuma Sumarauw 201110330311097
UNIVERSITAS MUHAMMADIYAH MALANG
FAKULTAS KEDOKTERAN
2015
HASIL PENELITIAN
PENGARUH EKSTRAK BIJI KAKAO (THEOBROMA CACAO L.)
TERHADAP KUALITAS SPERMATOZOA TIKUS PUTIH (RATTUS
NORVEGICUS) JANTAN YANG DIINDUKSI ALLOXAN
SEBAGAI AGEN HIPERGLIKEMIK
KARYA TULIS AKHIR
Diajukan kepada
Universitas Muhammadiyah Malang
Untuk Memenuhi Salah Satu Persyaratan
Dalam Menyelesaikan Program Sarjana
Fakultas Kedokteran
Oleh
Pamela Andria Putri Kusuma Sumarauw
201110330311097
UNIVERSITAS MUHAMMADIYAH MALANG
FAKULTAS KEDOKTERAN
2015
KATA PENGANTAR
Puji syukur saya panjatkan kepada Tuhan Yang Maha Esa, karena atas berkat
dan rahmat-Nya, penulis telah berhasil menyelesaikan penelitian yang
berjudul“Pengaruh Pemberian Ekstrak Biji Kakao (Theobroma Cacao L.) Terhadap
Kualitas Spermatozoa Tikus Putih (Rattus Norvegicus) Jantan Yang Diinduksi
Alloxan Sebagai Agen Hiperglikemik”. Penulisan penelitian ini dilakukan dalam
rangka memenuhi salah satu syarat untuk mencapai gelar Sarjana Kedokteran Jurusan
Pendidikan Dokter pada Fakultas Kedokteran Universitas Muhammadiyah Malang.
Penulis menyadari bahwa penyusunan tugas akhir ini jauh dari sempurna,
walaupun demikian penulis telah berusaha semaksimal mungkin serta mendapatkan
bantuan dan bimbingan dari dosen pembimbing dalam rangka penyusunan. Tanpa
bantuan dan dorongan dari berbagai pihak, sangatlah tidak mudah menjalani masa
perkuliahan hingga pada penyusunan tugas akhir ini.
Akhir kata penulis berharap semoga penelitian ini dapat menambah wawasan
dan bermanfaat bagi semua pihak.
Malang, Februari 2015
Penulis
xi
DAFTAR ISI
Halaman
HALAMAN JUDUL ................................................................................................... i LEMBAR PENGESAHAN ......................................................................................... iii HALAMAN PERNYATAAN ORISINALITAS ..................................................... iv LEMBAR PENGUJIAN ............................................................................................. v KATA PENGANTAR ................................................................................................. vi UCAPAN TERIMA KASIH ...................................................................................... vii ABSTRAK .................................................................................................................... ix ABSTRACT ................................................................................................................... x DAFTAR ISI ................................................................................................................ xi DAFTAR TABEL ........................................................................................................ xiv DAFTAR GAMBAR ................................................................................................... xv DAFTAR SINGKATAN.............................................................................................. xvi DAFTAR LAMPIRAN ......................................................................................... xvii BAB 1 PENDAHULUAN ............................................................................................ 1
1.1 Latar Belakang ............................................................................................... 1 1.2 Rumusan Masalah ......................................................................................... 3 1.3 Tujuan Penelitian .......................................................................................... 3
1.3.1 Tujuan umum ...................................................................................... 3 1.3.2 Tujuan khusus ..................................................................................... 3
1.4 Manfaat Penelitian ......................................................................................... 4 1.4.1 Manfaat teoritis ................................................................................... 4 1.4.2 Manfaat aplikatif ................................................................................ 4
BAB 2 TINJAUAN PUSTAKA ................................................................................. 5 2.1 Organ Reproduksi Tikus Jantan ................................................................... 5 2.1.1 Anatomi testis tikus jantan ................................................................. 5 2.1.2 Spermatogenesis …………………………………………………..7 2.1.3 Spermatozoa ........................................................................................ 10 2.2 Pemeriksaan Analisis Semen …………………………………………….12 2.2.1 Pemeriksaaan makroskopis ………………………………………..12 2.2.2 Pemeriksaan mikroskopis .................................................................... 13 2.3 Peroksidasi Lipid Spermatozoa .................................................................... 16 2.4 Diabetes Mellitus ........................................................................................... 17 2.4.1 Definisi ................................................................................................ 17 2.4.2 Klasifikasi ........................................................................................... 18 2.4.2.1 Diabetes mellitus tipe 1 .......................................................... 18 2.4.2.2 Diabetes mellitus tipe 2 .......................................................... 18 2.4.3 Kriteria diagnosis ................................................................................ 18 2.5 Alloxan ........................................................................................................... 19 2.5.1 Definisi ................................................................................................ 19
xii
2.5.2 Peran alloxan dalam pembentukan ROS ........................................... 19 2.6 Pengaruh Alloxan Terhadap kerusakan spermatozoa ................................. 20 2.7 Theobroma Cacao L. (Kakao) ...................................................................... 21 2.7.1 Taksonomi ............................................................................................ 23 2.7.2 Kandungan gizi biji kakao................................................................... 23 2.7.3 Manfaat biji kakao terhadap sistem reproduksi jantan ...................... 24 2.8Metode Ekstraksi ............................................................................................. 25 2.8.1 Definisi ................................................................................................... 25 2.8.2 Jenis metode ekstraksi ........................................................................... 25 BAB 3 KERANGKA KONSEPTUAL DAN HIPOTESIS PENELITIAN .......... 28 3.1 Kerangka Konseptual Penelitian ................................................................... 28 3.2 Hipotesis Penelitian ........................................................................................ 30 BAB 4 METODE PENELITIAN ............................................................................... 31 4.1 Jenis dan Rancangan Penelitian .................................................................... 31 4.2 Lokasi dan Waktu Penelitian ......................................................................... 31 4.3 Populasi dan Sampel ...................................................................................... 31 4.3.1 Populasi ................................................................................................ 31 4.3.2 Sampel .................................................................................................. 31 4.3.3 Besar sampel ........................................................................................ 31 4.3.4 Teknik pengambilan sampel ............................................................... 32 4.3.5 Karakteristik sampel penelitian........................................................... 32 4.3.6 Variabel penelitian ............................................................................... 33 4.3.6.1 Variabel bebas ......................................................................... 33 4.3.6.2 Variabel tergantung ................................................................ 33 4.3.7 Definisi operasional ............................................................................ 33 4.4 Alat dan Bahan Penelitian.............................................................................. 36 4.5 Prosedur Penelitian ......................................................................................... 37 4.6 Kerangka Operasional .................................................................................... 41 4.7 Analisis Data................................................................................................... 42 BAB 5 HASIL PENELITIAN DAN ANALISIS DATA ......................................... 43 5.1 Hasil penelitian .............................................................................................. 43 5.1.1 Kadar gula darah .................................................................................. 43 5.1.2 Kualitas spermatozoa ........................................................................... 45 5.2 Analisis data ................................................................................................... 47 5.2.1 Uji normalitas dan homogenitas ......................................................... 47 5.2.2 Analisis one way anova ....................................................................... 49 5.2.3 Analisis post hoc test ........................................................................... 51 5.2.4 Korelasi pearson .................................................................................. 53 5.2.5 Regresi linier sederhana ..................................................................... 54 BAB 6 PEMBAHASAN ............................................................................................... 60 6.1 Pembahasan .................................................................................................... 60 6.2 Keterbatasan penelitian .................................................................................. 63 BAB 7 KESIMPULAN DAN SARAN ....................................................................... 64
7.1 Kesimpulan ..................................................................................................... 64 7.2 Saran……… ................................................................................................... 64
xiii
DAFTAR PUSTAKA ................................................................................................... 65 LAMPIRAN……….. .................................................................................................... 73
xvii
DAFTAR LAMPIRAN
Lampiran Halaman
1 Gambar hasil penelitian..............................................................................................73
2 Hasil analisis data .......................................................................................................77
3 Dokumentasi Penelitian .............................................................................................90
4 Surat Determinasi Tanaman .......................................................................................91
65
DAFTAR PUSTAKA
Agarwal, A., Prabakaran, S., Said, T., 2005, Prevention of oxidative stress injury
to sperm. J Androl, 26, 60-654
Akbar B, 2010, Reproduksi Hewan Jantan, In: Tumbuhan Dengan Kandungan
Senyawa Aktif Yang Berpotensi Sebagai Bahan Antifertilitas, Adabia Press,
Jakarta, pp 15-17
Al-Haija, 2011, Main Causes of Infertility among Men Treated at Razan Centers
in West Bank: Retrospective study, An-Najah National University, Palestine
American Diabetes Association, 2015, Standards of Medical Care In Diabetes –
2015, The Journal of Clinical And Applied Research And Education, Vol.
38 (1), pp S1-S2
Andujar, M.C, 2012, Review Article: Cocoa Polyphenols and Their Potential
Benefits for Human Health, Facultat de Farmacia University de Valencia,
Spain
Anerao, Sharma, Rahtore, 2010, Studies On Human Sperm Motility and Viability
When Treatment With Rock Salt, Department of Physiology, M.G.M
Medical College, Vol.01, pp 01-10
Arief S, 2012, Radikal bebas, Bulletin Pediatrik Universitas Airlangga, Vol.1, pp
1-9. Surabaya
Ashfahani ED, Wiratmini NI, Sukmaningsih, 2010, Motilitas dan Viabilitas
Spermatozoa Mencit (Mus musculus L) Setelah Pemberian Ekstrak Temu
Putih (Curcuma zedoaria (Berg.) Roscoe), Jurnal Biologi, Vol. XIV (1), pp.
20 – 23
66
Asa Anindito GP, 2013, Pengaruh Pemberian Ekstrak Biji Anggur (Vitis Vinifera
L.) terhadap Motilitas dan Viabilitas Spermatozoa Tikus Putih (Rattus
Norvegicus Strain Wistar) Jantan yang Diberi Paparan Asap Rokok,
Fakultas Kedokteran Universitas Muhammadiyah Malang, Malang
Bashandy AE, 2007, Effect of Fixed Oil of Nigella Sativa on Male Fertilty in
Normal and Hyperlipidemic Rats, International Journal of Pharmacology,
Vol. 2 (1), pp 27-33
Boitrelle et al, 2013, The Nature of Human Sperm Head Vacuoles: A Systematic
Literature Review, Basic and Clinical Andrology, Vol.23 (2)
Chandrashekar, KN., Muralidhara., 2009, Evidence of oxidative stress and
mitochondrial dysfunctions in the testis of prepubertal diabetic rats. [Int J
Impot Res] 2009 May-Jun; Vol. 21 (3), pp. 198-206
Christijanti W, Utami NR, Iswara A, 2010, Efek Pemberian Antioskidan Vitamin
C dan E Terhadap Kualitas Spermatozoa Tikus Putih Terpapar Alletherin,
Biosaintifika, Vol.2 (10), pp. 18 -26
De Jonge, Barratt, 2006, The Sperm Cell : Production, Maturation, Fertilization,
Regeneration, Cambridge University Press, New York
Dewi FY, Anthara MS, Dharmayudha GO, 2014, Efektifitas Ekstrak Daun Sirih
Merah (Piper crocatum) Terhadap Penurunan Kadar Glukosa Darah Tikus
Putih Jantan (Rattus norvegicus) Yang Diinduksi Aloksan, Buletin Veteriner
Udayana, Vol.6 (1)
Dewi Wiryanthini IA, Aman IGM, Bagiada NA, 2012, Pemberian Ekstrak Biji
Kakao (Theobroma cacao L) Menurunkan Kadar Malondialdehide dan
Meningkatkan Kadar NOx Darah Tikus Putih (Rattus Norvegicus) yang
67
Diinduksi Stres Psikososial, Jurnal Ilmiah Kedokteran : Medicina, Vol. 43
(3)
Dharma SH, 2012, Peranan Antioksidan Endogen dan Eksogen Terhadap
Kesehatan, Cermin Dunia Kedokteran, Vol. 39 (10), pp 793 – 794
Dirjen POM, 2000, Paramaeter Standar Umum Ekstrak Tumbuhan Obat, Cetakan
Pertama, Jakarta: Departemen Kesehatan RI, pp 10 - 12
Erniati, Zakaria FR, Priosoeryanto BP, 2012, Efek Konsumsi Minuman Bubuk
Kakao (Theobroma Cacao L.) Bebas Lemak Terhadap Sifat Antioksidatif
Limfosit Subyek Perempuan, Jurnal Tekhnologi dan Industri Pangan, Vol.
XXIII (1), pp 81-85
Erukainure OL, Okafor OY, Obode OC et.al, 2012, Blend of Rossele Calyx and
Selected Fruits Modulates Testicular Redox Status an Sperm Quality of
Diabetic Rats, Journal Diabetes Metabolism,Vol.3(8), pp 214-218
Escriba PV, Nicolson GL, 2014, Membrane Structure and Function: Relevance of
Lipid and Protein Structures In Cellular Physiology, Pathology and
Therapy, Biochimica et Biophysica Acta, Vol. 1838, pp 1449–1450
Esmaeel AK, 2013, Relationships Between Diabetes Mellitus Type 2 and Male
Testosterone Level, Journal of Babylon University/Pure and Applied
Sciences No.(1), Vol.(21)
Evenson DP, Larson KJ, Jost LK, 2002, Sperm Chromatin Structure Assay: Its
Clinical Use for Detecting Sperm DNA Fragmentation In Male Fertility And
Comparisons with Another Techniques, Journal of Andrology, Vol. 23, pp
25 - 43
68
Faranita OV, 2009, Kualitas Spermatozoa Pada Tikus Wistar Jantan Diabetes
Mellitus, Fakultas Kedokteran Universitas Diponegoro, Semaran
Fauci A, Braunwald E, Kasper D, Hauser S, 2008, Harrison's Principles of
Internal Medicine 17th Edition, McGraw-Hill Medical, New York
Hafiz, R, 2008, Artikel Karya Ilmiah : Pengaruh Pemberian Minyak Jinten Hitam
(Nigella sativa)Terhadap Morfologi Spermatozoa Mencit Yang Diinduksi
oleh Alloxan, Fakultas Kedokteran Universitas Diponegoro, Semarang
Hertansa RM, 2010, Pengaruh Pemberian Dutch Style Cocoa Powder Terhadap
Kadar Glukosa Darah Tikus Putih (Rattus norvegicus) yang Diinduksi
Alloxan, Fakultas Kedokteran Universitas Sebelas Maret, Surakarta
Hidayah, Nurul, 2007, Identifikasi dan Pengelolaan Stress Infertil, Konferensi
Nasional Stress Management dalam Berbagai Setting Kehidupan, Bandung
Hii CL, Law CL, Suzannah S, Misnawi, Cloke M, 2009, Polyphenols in Cocoa
(Theobroma cacao L), Asian Journal of Food and Agro-Industry, Vol. 2 (4),
pp 702 – 722
Inveresk Research, 2000, Rat Sperm Morphological Assesment, Industrial
Reproductive Toxicology Discussion Group, Vol.1, pp 6 – 13
Karmawati E, Mahmud Z, Syakir M, Rubiyo, 2010, Budidaya dan Pasca Panen
Kakao, Pusat Penelitian dan Pengembangan Perkebunan, diunduh pada 16
September 2014 (perkebunan.litbang.deptan.go.id)
Khardori R, 2013, Changing Paradigm In Type 2 Diabetes Mellitus, Indian
Journal of Endocrinology and Metabolism, pp 68-71
69
Maneesh, M., 2006, Impaired Hypothalamic-Pituitary-Gonadal Axis Function in
Men With Diabetes Mellitus, Indian Journal of Clinical Biochemistry,
Vol.21 (1), pp 165-168
Meldawati, 2011, Pengaruh Ekstrak Buah (Morinda Citrifolia Linn) Terhadap
Kualitas, Kuantitas Sperma Dan Kadar Malondialdehyde Testis Tikus
Wistar Diabetes Mellitus, Fakultas Kedokteran Universitas Sumatera Utara,
Medan
Miyamoto, T, Tsujimura A, Miyagawa Y, Namiki M, 2012, Review Article : Male
infertility and Its Causes in Human, Advances in Urology Ashikawa
Medical University, Japan
Muchtadi D, 2013, Antioksidan & Kiat Sehat di Usia Produktif, Alfabeta,
Bandung
Nawaekasari M, 2012, Efek Senyawa Polifenol Ekstrak Biji Kakao (Theobroma
cacao L) Terhadap Pertumbuhan Bakteri Lactobacillus acidophilus,
Fakultas Kedokteran Gigi Universitas Jember
Nugroho AE, 2006, Hewan Percobaan Diabetes Mellitus: Patologi dan
Mekanisme Aksi Diabetogenik, Biodiversitas, 7(4), 378-382.
Oliveira H, Marcello S, Santos C, 2009, Adverse Effect of Cadmium Exposure On
Mouse Sperms, Reproductive Toxicology, Elsevier Ltd, Vol. 28, pp 550-555
Oryza Oil & Fat Chemical CO LTD, 2011, Cocoa Extract : Ingredient For
Weight Management with Natural Antioxidants, Tokyo
Paoletti R, Polli A, Conti A, Visioli F, 2011, Chocolate and Health, Springer,
Milan
70
Pebrianti Lina NM, 2011, Kualitas Spermatozoa Mencit Jantan Dewasa (Mus
musculus L.) Setelah Diberikan Monosodium Glutamat (MSG), Jurnal
Simbiosis Udayana, Vol. 1 (1), pp. 40 – 50
PERKENI, 2007, Guidelines For Management of Postmeal Glucose
Porbowaseso, 2014, Ekstraksi Polifenol Biji Kakao Secara Kimiawi Sebagai
Antioksidan dan Pewarna Alami, Fakultas Teknologi Pertanian Universitas
Jember, Jember
Purwaningsih, Agung W, Megaputera I, 2013, Formulasi Sediaan Ekstrak Etanol
Biji Kakao (Theobroma Cacao L.) Sebagai Kandidat Natural Antioxidant
Melalui Teknologi Mikroenkapsulasi Dengan Metode Spray-Drying,
Fakultas Farmasi Universitas Hasanuddin, Makasar (artikel.dikti.go.id
diunduh tanggal 18 Okteber 2014)
Septiana AT, Asnani A, 2012, Kajian Sifat Fisikokimia Ekstrak Rumput Laut
Coklat (Sargassum Duplicatum) Menggunakan Berbagai Pelarut Dan
Metode Ekstraksi, Jurnal AGROINTEK, Vol. 6(1), pp 22-28
Setiawan M, 2008, Buku Ajar Endokrin, Bab 2:17, UMM Press, Malang
Sherwood L, 2007, Human Physiology: From Cells To System 6thEdition, Chapter
20:819-824, EGC, Jakarta
Smit M, 2011, Clinical Application of Sperm Chromatin Structure Assesment in
Andrology Patients, Erasmus Universiteit Rotterdam, Rotterdam
Snell R, 2006, Anatomi Klinik untuk Mahasiswa Kedokteran Edisi 6, Bab 7:349-
350, EGC, Jakarta
Supranto J, 2007, Teknik Sampling untuk Survei dan Eksperimen, Rineka Cipta,
Jakarta
71
Tanagho E. A., McAninch, J. W., 2008. Smith's General Urology Vol. 17, Bab
44:689-691, McGraw-Hill Medical, New York
Tapas AR, Sakarkar DM, Kakde RB, 2008, Flavonoids as nutraceuticals: A
review, Tropical Journal of Pharmaceutical, Vol.7(3), pp 89-93
Trindade A, Simoes A, Silva R et.al, 2013, Long Term Evaluation of
Morphometric and Ultrastructural Changes of Testes of Alloxan-induced
Diabetic Rats, Acta Cirurgica Brasileira, Vol.28(4), pp 256-265
Twsigye CK, 2011, Cell Biology And Genetics, Chapter 4:23-24 African Virtual
University, Africa
WHO, 2011, Laboratory Manual For The Examination and Processing of Human
Semen, Edisi 5, pp 22
Wibisono H, 2010, Buku Pertama : Panduan Laboratorium Andrologi, Bab 1:7-
10, Refika Aditama, Bandung.
Widowati, 2008, Potensi Antioksidan Sebagai Anti Diabetes, Jurnal Kesehatan
Masyarakat, Vol.7 (2), pp 1 – 10
Winarni S, Judiwati R, Prajogo B, Hayati A, 2011, Fraksi Etanol 96% Biji Koro
Benguk (Mucuna pruriens L.) Sebagai Peningkat Kualitas Spermatozoa
Mencit (Mus Musculus), Jurnal Kesehatan Reproduksi, Vol.1 (2), pp 60 – 66
Winarsi H, 2007, Antioksidan Alami dan Radikal Bebas, Kanisius, Yogyakarta
Yudiarti T, Widiastuti E, Pratikno H, 2004, Buku Ajar Biologi, Fakultas
Peternakan Universitas Diponegoro, Semarang
Yulianto RA, Isnaeni W, Susanti R, 2013, Pengaruh Pemberian Vitamin E
Terhadap Kualitas Sperma Tikus Putih Yang Dipapar Timbal, Unnes
Journal of Life Science, Vol.2 (2)
72
Yuriska A, 2009, Efek Aloksan Terhadap Kadar Glukosa Darah Tikus Wistar,
Fakultas Kedokteran Universitas Diponegoro, Semarang
Zadda A, 2009, Pengaruh Diet Rumput Laut Eucheuma Sp. Terhadap Jumlah
Eritrosit Tikus Wistar dengan Diabetes Aloksan, Fakultas Kedokteran
Universitas Diponegoro, Semarang
Zare S, Rahmani Y, Eftekhaari TE, Faramarzi A, 2013, Role of oxidative stress in
Male infertility, International Electronic Journal of Medicine (IEJM), Vol. 2
(2), pp 77-81