uraAupp

purM ppk purN

pAQ5pAQ5-1pAQ5-2pInter

upp purM

ACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTAT

ACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAA

AATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTATG

AAAGAAGATTATTCCATCCGAAAACTAACCTTTACCCTGGCACAAGT

CTTCTTTCGCCGCGCGCCTGGGGAAAAGACGTGCAAAAAGGTTGTGT

AAAGCAGTCTCGCAAACGTTTGCTTTCCCTGTTAGAATTGCGCCGAA

TTTTATTTTTCTACCGCAAGTAACGCGTGGGGACCCAAGCA

FNR

PurR

A

B

Deletion of FNR binding site in pInterD1 C

Deletion of PurR binding site in pInterD2