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SPEKTROFOTOMETRI UV
MULTIKOMPONEN
PENETAPAN KADAR INH
DAN VITAMIN B6
MAKALAH ANALISIS INSTRUMENTAL
MODUL IV
SPEKTROFOTOMETRI UV MULTIKOMPONEN
PENETAPAN KADAR INH DAN
VITAMIN B6
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Disusun Oleh:
Kelompo : A !An""o#$ : %& E$ Se#i$nis$ 'K %(( %%( (()&
*& E$ P+$sn$ P, 'K %(( %%( (%-&
-& D.$h Ris/$+i Pi#$lo$ S, 'K %(( %%( (-6&
0& El1esi Me1is$ I, 'K %(( %%( (-)&
Ko+e#o+ :
LABORATORIUM ANALISIS INSTRUMENTAL
FAKULTAS FARMASIUNIVERSITAS MUHAMMADI2AH SURAKARTA
*(%*
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ABSTRAK
Me#o1e pe+s$m$$n simul#$n 1il$u$n 1en"$n men"uu+ * $#$u le3ih p$n4$n"
"elom3$n" .$n" m$n$ m$sin"5m$sin" omponen #i1$ $$n men"$n""u, K+omoo+
.$n" 3e+3e1$53e1$ 1$+i #i$p omponen $$n mempun.$i eu$#$n $3so+3s 7$h$.$ .$n"
3e+3e1$ pul$ p$1$ s$#u 1$e+$h p$n4$n" "elom3$n", Pen"ui+$n 1il$u$n p$1$
m$sin"5m$sin" l$+u#$n p$1$ #i$p p$n4$n" "elom3$n" 'menu+u# 3$n.$n.$ omponen&8sehin""$ 1ipe+oleh pe+s$m$$n hu3un"$n $n#$+$ $3so+3$nsi 1en"$n onsen#+$si p$1$
p$n4$n" "elom3$n" #e+se3u#,
PENDAHULUAN
DASAR TEORI
Spe#+oo#ome#+i se+$p $1$l$h pen"uu+$n se+$p$n +$1i$si ele#+om$"ne#i
p$n4$n" "elom3$n" #e+#en#u .$n" smpi#8 men1e$#i mono+om$#i .$n" 1ise+$p 9$#,
Pen"uu+$n se+$p$n 1$p$# 1il$u$n p$1$ 1$e+$h ul#+$iole# ' %;( < -)( nm& $#$u
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p$1$ 1$e+$h 7$h$.$ #$mp$ ' -)( < =)( nm&, D$.$ se+$p $#$u se+$p$n 4enis 9$# p$1$
3$#$s53$#$s $1$+ #e+#en#u $1$l$h #e#$p8 #i1$ #e+"$n#un" 1$+i in#ensi#$s +$1i$si8 p$n4$n"
4$l$n sin$+ 1$n $1$+ l$+u#$n8 sehin""$ spe#+oo#ome#+i se+$p 1$p$# 1i"un$$n un#u
pene#$p$n $1$+, Al$# spe#+oo#ome#e+ p$1$ 1$s$+n.$ #e+1i+i $#$s sum3e+ sin$+ mono+om$#o+8
#emp$# sel un#u 9$# .$n" 1ipe+is$8 1e#e7#o+8 pen"u$# $+us8 1$n $l$# uu+ $#$u pen7$#$#,
Spe#+oo#ome#e+ 1$p$# 3ee+4$ se7$+$ o#om$#i $#$upun #i1$8 1$p$# mempun.$i s.s#em
sin$+ #un""$l $#$u "$n1$,
'Anonim8 %;=;&
B$"i$n moleul .$n" men"$3so+3si 1$l$m 1$e+$h ul#+$iole# 1$n 1$e+$h sin$+ #$mp$
1in.$#$$n se3$"$i +omoo+, D$l$m s$#u moleul 1$p$# 1i$n1un" 3e3e+$p$ +omoo+,
>i$ +omoo+ 1ipis$h$n s$#u s$m$ l$in p$lin" se1ii# oleh 1u$ $#om $+3on 4enuh8 m$$
#i1$ $1$ emun"in$n $1$n.$ on4u"$si $n#$+$ "u"us +omoo+,
'He+m$nn 1$n ?o##+ie18 %;;)&
Aspe Ku$li#$#i
D$#$ spe#+$ UV se7$+$ #e+sen1i+i #i1$ 1$p$# 1i"un$$n un#u i1en#ii$si
u$li#$#i o3$# $#$u me#$3oli#n.$, A$n #e#$pi 1i"$3un" 1en"$n 7$+$ l$in sepe+#i
Spe#+os#opi In+$me+$h8 +eson$nsi m$"ne# in#i8 1$n spe#+os#opi m$ss$8 1$p$# 1i"un$$n
un#u m$su1 i1en#ii$si@$n$lisis u$li#$#i sen.$/$ #e+se3u#,
Aspe Ku$n#i#$#i
D$l$m $spe u$n#i#$#i8 su$#u 3e+$s +$1i$si 1ien$$n p$1$ 7upli$n 'l$+u#$n
s$mpel& 1$n in#ensi#$s sin$+ +$1i$si .$n" 1i#e+us$n 1iuu+ 3es$+n.$,
An$lisis * 7$mpu+$n se7$+$ 3e+s$m$5s$m$
Bil$ 1iin"in$n pen"uu+$n * 3u$h sen.$/$ se7$+$ 3e+s$m$5s$m$ se7$+$
spe#+oo#ome#+i8 m$$ 1$p$# 1il$u$n p$1$ * p$n4$n" "elom3$n" .$n" m$n$ m$sin"5
m$sin" omponen #i1$ s$lin" men""$n""u $#$u "$n""u$n 1$+i omponen .$n" l$in
p$lin" e7il, Du$ 3u$h +omoo+ .$n" 3e+3e1$ $$n mempun.$i eu$#$n $3so+3si
7$h$.$ .$n" 3e+3e1$ pul$ p$1$ s$#u 1$e+$h p$n4$n" "elom3$n", Pen"uu+$n 1il$u$n
p$1$ m$sin"5m$sin" l$+u#$n p$1$ 1u$ p$n4$n" "elom3$n"8 sehin""$ 1ipe+oleh 1u$
pe+s$m$$n hu3un"$n $n#$+$ $3so+3$nsi 1en"$n onsen#+$si p$1$ 1u$ p$n4$n"
"elom3$n"8 $i3$#n.$ onsen#+$si m$sin"5m$sin" omponen 1$p$# 1ihi#un",
'?$n14$+8 *((=&
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TU>UAN
P+$#i$n 1ih$+$p$n m$mpu mene#$p$n $1$+ INH 1$n i#$min B6 1$l$m
s$mpel .$n" men"$n1un" 1u$ omponen $#$u le3ih men""un$$n $l$# spe#+oo#ome#e+
UV 1en"$n me#o1e pe+s$m$$n simul#$n,
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METODOLO?I
ALAT DAN BAHAN
5 Al$#
%, Spe#+oo#ome#e+ UV
*, Kue#-, L$3u #$$+ %( mL
0, Bee+ "l$ss %(( mL
!, Pipe# olume
6, Mi+opipe#
=, P+opipe#
), E+lenme.e+
;, Tim3$n"$n $n$li#i
%(, Pipe# #e#es
5 B$h$n
%, T$3le# .$n" men"$n1un" INH 1$n i#$min B6
*, Au$1es#
-, L$+u#$n 3$u INH (8%
0, L$+u#$n3$u Vi#$min B6 (8%
PROSEDUR RESMI
Vi#$min B6 'Pi+i1oCin Hi1+olo+i1$&
?olon"$n $n$lisis : V
: )H%*NO-l '*(!86&8 #i#i leleh *%(o8 3u3u +is#$l pu#ih8 #$ 3e+3$u8 +$s$ $s$m5p$hi#,
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Kel$+u#$n
1$l$m
$i+ E#$nol $se#on E#e+ lo+oo+m
1 : 8 1 : 100 Tak larut Tak larut Tak larut
Pemeriksaan kualitatif:%& Re$si 3esi 'III& lo+i1$ : /$+n$ me+$h
*& Ke 1$l$m 7$mpu+$n * mL l$+u#$n $s$m sul$nil$# #e+1i$9o#$si 1$l$m % mL - N N$OH
1i#$m3$h i+$(i+$ ! m" 9$#, L$+u#$n 3e+/$+n$ unin" #u$ s$mp$i 4in""$ emu1i$n
1i#$m3$h$n * mL - N $s$m $se#$#8 3e+u3$h men4$1i me+$h,
-& Ke 1$l$m l$+u#$n !( m" 9$# 1$l$m % mL $i+ 1i#$m3$h$n % #e#es l$+u#$n #em3$"$ sul$#
* 1$n % mL - N N$OH8 #e+3en#u /$+n$ 3i+u un"u,
0& Se4uml$h % m" 9$#8 1il$+u#$n 1$l$m %( mL $i+8 e 1$l$m % mL l$+u#$n 1ilo+inon
lo+emi1$ (8(0 1$l$m e#$nol mu#l$ 1$n % #e#es l$+u#$n 6 N $moni$8 #e+3en#u /$+n$
3i+u8 e 1$l$m % mL l$+u#$n l$inn.$8 1i#$m3$h l$+u#$n $s$m 3en9o$#e - % mL
1ilo+inon lo+imi1$ 1$n % #e#es l$+u#$n $moni$8 #i1$ #im3ul /$+n$ 3i+u,
Pemeriksaan kuantitatif:
%& Ti#+$si : L$+u#$n 9$# 1$l$m $s$m $se#$# '1i#$m3$h$n l$+u#$n +$s$ 'II& $se#$#& 1i#i#+$si
1en"$n (8(- N $s$m pe+lo+$# '%@*( mmol& s$mp$i #im3ul /$+n$ 3i+u, In1i$#o+ - #e#es
l$+u#$n n"u K+is#$l,
*& 1$l$m $i+ : **( p$1$ *!0 nm
0*! p$1$ -*0 nm,
Isoni$9i1 'INH&
?olon"$n $n$lisis : IV8 V
: 6H=H-( '%-=8%&8 4$+$ 7$i+ %=( < %=0o8 K+is#$l pu#ih8 +$s$ p$hi#,
Kel$+u#$n
1$l$m
$i+ E#$nol $se#on E#e+ lo+oo+m
1 : 8 1 : 45 1 : 1000 Tak larut 1 : 1000
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Pemeriksaan kualitatif:
%& Isoni$9i1 'INH& me+e1usi l$+u#$n Pe+$ Ni#+$#8 Amoni$8 pe+e$si Fehlin"8 1$n l$+u#$n
K$lium Pe+m$n"$n$# 1in"in,
*& L$+u#$n !( m" 9$# 1i+e$si$n 1en"$n % mL l$+u#$n *80 1i5ni#+olo+o3en9ol '%8( "+@%((
m*e#$nol 1i3u$# se"e+$& 1$n 0 #e#es - N N$OH, D$l$m /$#u sin"$#8 #e+3en#u l$+u#$n
me+$h 7ol$# .$n" #i1$ 3e+u3$h se$lipun 1ien7e+$n 1en"$n $i+,
-& Se4uml$h *( m" 9$# 1$n %(( m" N$#+ium K$+3on$# e+in" 1ip$n$s$n p$1$ $7$
po+selen8 #e+7ium 3$u pi+i1in,
Pemeriksaan kuantitatif:
%& Ti#+$si : L$+u#$n 9$# 1$l$m %) mL As$m Ase#$# 3e3$s $i+ 1$n * mL Anhi1+i1$ Ase#$#
1i#i#+$si 1en"$n (8(! N As$m Pe+lo+$# '%@*( mmol& s$mp$i #im3ul /$+n$ hi4$u, In1i$#o+
% #e#es l$+u#$n un"u +is#$l,*& 1$l$m $i+ : -)( p$1$ *66 nm,
ARA KER>A
%, Menen#u$n @ Men7$+i H$+"$
INH 1$l$m $i+ -)( p$1$ *66 nm
Vi#$min B6 1$l$m $i+ **( p$1$ *!0 nm
0*! p$1$ -*0 nm
*, A, Pene#$p$n L$+u#$n S#o INH (8%
Di#im3$n" %(( m" INH,
Dim$su$n e 1$l$m l$3u #$$+,
Di#$m3$h pel$+u# $i+ $1 %(( mL,
Di3u$# l$+u#$n s#o 3$u INH (8% ,
Nb: sudah tersedia di laboratorium
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B, Pene#$p$n L$+u#$n S#o Vi#$min B6 (8%
Di#im3$n" %(( m" Vi#$min B6,
Di#$m3$h pel$+u# $i+ $1 %(( mL,
Di3u$# l$+u#$n s#o 3$u Vi#$min B6 (8% ,
Nb: sudah tersedia di laboratorium
-, A, Di#en#u$n m$C
Di$m3il %(( L l$+u#$n s#o INH (8% 3@
Dim$su$n e 1$l$m l$3u #$$+ %( mL,
Di#$m3$h pel$+u# $i+ $1 %(8( mL,
Dim$su$n e 1$l$m ue#,
Diuu+ +es$p$nn.$ p$1$ 3e+3$"$i p$n4$n" "elom3$n" un#u menen#u$n m$C,
+$n"e : *(( < 0(( nm,
B, Di$m3il %(( L L$+u#$n S#o Vi#$min B6 (8%
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Dim$su$n 1$l$m l$3u #$$+ %( mL,
Di#$m3$h $u$1es# $1 %( mL,
Dim$su$n e 1$l$m ue#,
Diuu+ +es$p$nn.$ p$1$ 3e+3$"$i p$n4$n" "elom3$n" un#u menen#u$n m$C,
0, A, Pen"uu+$n A3so+3$nsiDi$m3il %(( L l$+u#$n s#o INH (8% 3@
Dim$su$n e 1$l$m l$3u #$$+ 1$n 1i#$m3$h$n $i+ $1 %( mL,
Dim$su$n e 1$l$m ue#,
Di3$7$ $3so+3$nsin.$ p$1$ se#i$p p$n4$n" "elom3$n" m$C p$1$ INH '*6! nm&
1$n Vi#$min B6 '-*08! nm&,
B, Di$m3il *(( L L$+u#$n S#o Vi#$min B6 (8% ,
Dim$su$n 1$l$m l$3u #$$+ 1$n 1i#$m3$h$n $i+ $1 %( mL,
Dim$su$n e 1$l$m ue#,
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Di3$7$ $3so+3$nsi p$1$ m$C INH '*6! nm& 1$n m$C Vi#$min B6 '-*08! nm&,
Dim$su$n 1$#$ $3so+3$nsi #e+se3u# e 1$l$m * pe+s$m$$n .$n" 1i1$p$#,
Diseles$i$n pe+s$m$$n #e+se3u# 1en"$n me#o1e elimin$si un#u men7$+i $1$+ sen.$/$
.$n" 1i$n$lisis,
HASIL DAN PEMBAHASAN
Senyawa Vitamin B6 Senyawa INH
220 pada 254 nm
425 pada 324 nm
320 pada 266 nm
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)*+ '9
INH
INH
0(001 *
0(001 *
0(374
0(372
0(037
0(045
Vi#, B6
Vi#, B6
0(002 *
0(002 *
0(2
0(315
0(752
0(771
;ata
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D$#$ Repli$si
Berat )m&+
)!+
Berat )m&+
)B+
Berat )m&+
)+
Berat kerta# 0(2682 0(2670 0(2650
Berat kerta# - .at 0(3682 0(3672 0(3655
Berat kerta# - .at
#i#a0(264 0(2672 0(2654
Berat .at 0(0 0(1000 0(1001
!dd10 m$
$arutan yan&
di'aa 25
$ ad 10 m$
=p400
Nilai !'#%r'an#i )!+
ada m
a 1 )265(0
nm+
ada m
a 2 )324(5
nm+
; I ! 0(631> 0(727 0(035> 0(040
; II B 0(67> 0(55 0(032> 0(02
; III 0(746> 0(575 0(041> 0(026
R$#$5+$#$ :
m$C %
R I (86=;
R II (86%; *6! nm
R III (866%
m$C %
R I (8(-=!
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R II (8(-(! -*08! nm
R III (8(--!
Konsen#+$si $/$lA A =!80= 3@ B 5%8(-6 , %(
50 3@
B B 5! 3@3 A =)8!0 3@
A =08-% 3@3 B 508%00 3@3
PERHITUN?AN
O+ien#$si
(86-* -=- A %!-8! B -)%
(8(0* 0% A -)% B %!-8!
*0(8=;* %0*,%%- A !),0)-8! B
6800= 6,00=
A !),0)-8! B J
*0-8-0! %-!666 A
A %8=*= %(5- 3@ 0((
A (86;% 3@ %((
%(( 6)86* 3@3
(8(0* 0% A -)% B
(8(0* 0% '%8=*= %(5-& -)% B
(8(0* (8(=% -)% 73
B 5=86%* %(5! 3@
%((
5=8!!; %(5! 3@3
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Repli$si I
(86-* -=- A %!-8! B -)%(8(-=! 0% A -)% B %!-8!
*!)86;; %0*,%%- A !),0)-8! B
!8=!6*! 6*;-8
! A !),0)-8! B J
*!*8;0- %-!0%;8! A
A %8)6) %(5- 3@ 0((
A %(( =!80=
%(( 6)86* 3@3
(8(-=! 0% '%8)6) %(5-& -)% B
(8(-=! (8(=6!) -)% B
5(8(-;() -)% B
5%8(*!= %(50
B5%8(*!= %(50 3@ B
5%(-6 %(50 3@3
508%00 3@3
Repli$si II
(86=; -=- A %!-8! B -)%
(8(-(! 0% A -)% B -=-
*=8)0 %!*;- A 6*;-8! B
%%8-) %!*8;
- A %0*%%- B J
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%6806 5%-!)%;8! B
B 5%8*%%; %(50 3@ 0((
(8(! 3@ (8(! 3@3 %(( 5! 3@3
(8(-(! 0% A -)% B
(8(-(! 0% A -)% '5%8*%%; %(50 &
(8(-(! 0% A '5(8(!&
A %8;6- %(5- 3@
%(( (8%;6 0((
=)8!0 3@3
Repli$si II
(866% -=- A %!-8! B -)%
(8(--! 0% A -)% B -=-
*!%8)0% %0*%%- A 6*;-8! B!8%0**! 6*;-8
! A %0*%%- B J
*!%8)-!) %-!0%;8! A
A %8)!;6 %(5- 3@
A %(( 5! 3@3
(8%)! 0(( =08-% 3@3
(8(--! 0% '%8)!;6 %(5-& -)% B
(8(--! (8(=6 -)% B
5%8%%! %(50 B
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5%8%%! %(50 B
B %(( 50806 3@3
K$1$+ INH +$#$5#+$#$
=68%(= 3@3K$1$+ Vi#$min B6 +$#$5+$#$
5-8(0)
3
@3EVALUASI HASIL
x x d? < d2
75(47
78(54
74(31
76(107
0(637
2(433
1(77
0(406
5(1
3(22
@umla d 4(867 d2 (554
1 %86**
SD *8%)6
D$#$ .$n" 1i7u+i"$i =)8!0 3@3
H$+"$ 1i#ol$ 4i$ *8!
%8! *8! =)8!0 3@3 (Jadi harga diterima)
P (8;!G n -G # *8-!-
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K$1$+ INH x #
=68%(= *8-!-
'=68%(= *8;6;=%& 3@3
K$1$+ Vi#$min B6
x x d? < d2
1(036 A 10
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5-8(0) *8-!-
'5-8(0) -86--& 3@3
PEMBAHASAN
P+$#ium ini 1il$u$n un#u mene#$pn$n $1$+ sen.$/$ 1$l$m s$mpel .$n"
men"$n1un" 1u$ ompone4 $#$u le3ih8 men""un$$n $l$# spe#+oo#ome#e+ UV 1en"$n
me#o1e pe+s$m$$n simul#$n,
P+$#iu ini men""un$$n me#o1e pe+s$m$$n simul#$n $+en$ me#o1e ini 1$p$#men"uu+ le3ih 1$+i s$#u omponen 1$l$m sen.$/$8 .$n" mempun.$i se+$p$n p$1$
p$n4$n" "elom3$n" .$n" 3e+1e$#$n8 h$l ini 1$p$# #e+4$1i $+en$ me#o1e pe+s$m$$n
simul#$n 1il$u$n 1en"$n men"uu+ p$1$ * $#$u le3ih p$n4$n" "elom3$n" .$n" m$n$
m$sin"5m$sin" omponen #i1$ $$n men"$n""u, K+omoo+ .$n" 3e+3e1$53e1$ 1$+i #i$p
omponen .$n" $$n mempun.$i eu$#$n $3so+3s 7$h$.$ .$n" 3e+3e1$ pul$ p$1$ s$#u
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1$e+$h p$n4$n" "elom3$n", Te#$pi #i1$ 1$p$# 1i"un$$n un#u sen.$/$ .$n"
mempun.$i "u"us +omoo+ s$m$,
S$mpel .$n" 1i"un$$n 1$l$h #$3le# .$n" men"$n1un" INH 'Isoni$9i1& 1$n
Vi#$min B6, INH 1$n Vi#$min B6 mempun.$i "u"us +omoo+ .$n" 3e+3e1$8 m$$ 1$#$
1i#e#$p$n $1$+n.$ men""un$$n me#o1e simul#$n, K+omoo+ $1$l$h "u"us $#$u s.s#em
.$n" 3e+#$n""un" 4$/$3 p$1$ pen.e+$p$n sin$+ .$n" me+up$$n "u"us #i1$ 4enuh .$n"
1$p$# men.e+$p +$1i$si 1$l$m 1$e+$h UV5Vis, Pen.e+$p$n se4uml$h ene+"i #e+se3u# $$n
men"h$sil$n #+$nsisi ele7#+on 1$n o+3i#$l 3e+esi#$si, Esi#$si $1$l$h #+$nsisi $#$u
pe+pin1$h$n ele7#+on 1$+i o+3i#$l +en1$h e o+3i#$l .$n" #in""i,
I9oni$9i1 mempun.$i h$+"$ 1$l$m pel$+u# $i+ $1$l$h -)( p$1$ *66
nm, 2$n" $+#in.$ Isoni$9i1 1en"$n $1$+ % 3@1i3$7$ $3so+3$nsin.$ p$1$ *66 nm
1en"$n ue# se#el$h % 7m $$n 1i1$p$# $3so+3$nsin.$ -)(, Se1$n"$n Vi#$min B6
mempun.$i h$+"$ 1$l$m pel$+u# $i+ **( p$1$ *!0 nm 1$n 0*! p$1$
-*0 nm,
L$+u#$n s#o 3$u INH 1$n Vi#$min B6 su1$h #e+se1i$ 1en"$n $1$+ %
3@1en"$n m$sin"5m$sin" pel$+u# $u$1es#, P$1$ penen#u$n m$C8 3e+1$s$+$n
h$+"$ 9$#8 un#u men"hi#un" 3e+$p$ onsen#+$si .$n" 1i"un$$n8 1ipe+oleh
onsen#+$si l$+u#$n INH .$n" 1i"un$$n $1$l$h (8((% 3@1$n 1i1$p$# m$C -*08!
nm 1en"$n $3so+3$nsi (8=0), Se1$n"$n onsen#+$si Vi#$min B6 .$n" 1i"un$$n $1$l$h
(8((* 3@1$n 1i1$p$# m$C *6!8( nm 1en"$n $3so+3$nsi (8-%*, Penen#u$n m$C
ini 1il$u$n p$1$ *(( nm < 0(( nm un#u UV,
P$1$ p+$#ium8 pem3$7$$n $3so+3$nsi 1il$u$n p$1$ m$C$+en$
epe$$nn.$ 4u"$ m$sim$l8 pe+u3$h$n $3so+3$nsi un#u se#i$p s$#u$n onsen#+$si
$1$l$h .$n" p$lin" 3es$+8 1i sei#$+ m$C8 3en#u u+$ $3so+3$nsin.$ 1$#$+ 'houm
L$m3e+#5Bee+ $$n #e+penuhi& 1$n 4i$ 1il$u$n pen"uu+$n ul$n" m$$ esl$h$n .$n"
ise3$3$n oleh pem$s$n"$n ul$n" p$n4$n" "elom3$n" $$n e7il se$li e#i$
1i"un$$n m$C,
P$1$ me#o1e pe+s$m$$n simul#$n8 pen"uu+$n 1il$u$n p$1$ m$sin"5m$sin"
l$+u#$n p$1$ #i$p p$n4$n" "elom3$n"8 sehin""$ 1ipe+oleh pe+s$m$n hu3un"$n $n#$+$
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$3so+3$nsi 1en"$n onsen#+$si, Dil$u$n pem3$7$$n $3so+3$nsi sen.$/$ INH 1en"$n
$1$+ (8((% 3@p$1$ m$C % '*6! nm& 1ipe+oleh $3so+3$nsi (8-=0 1$n m$C *
'-*08! nm& 1ipe+oleh $3so+3$nsi (8(-=, Pem3$7$$n 1il$u$n 1u$ $li, Den"$n $1$+ INH
(8((% 3@1i3$7$ p$1$ m$C % '*6! nm& 1ipe+oleh $3so+3$nsi (8-=* 1$n m$C *
.$i#u -*08! nm (8(0!, Dipe+oleh +$#$5+$#$ $3so+3$nsi un#u INH (8((% 3@ m$C %
'*6! nm& $1$l$h (8-=- 1$n p$1$ m$C * '-*08! nm& 1ipe+oleh $3so+3$nsi (8(0%, Un#u
pem3$7$$n $3so+3$nsi Vi#$min B6 $1$+ (8((* 3@p$1$ m$C % '*6! nm& 1ipe+oleh
$3so+3$nsi (8*;; 1$n p$1$ m$C * '-*08! nm& 1ipe+oleh $3so+3$nsi (8(=!*, Pem3$7$$n
1il$u$n * $li sepe+#i INH 1$n 1ipe+oleh $3so+3$nsi Vi#$min B6 p$1$ m$C % '*6!
nm& $1$l$h (8-%! 1$n p$1$ m$C * '-*08! nm& $1$l$h (8==%, D$n 1ipe+oleh $1$+ +$#$5
+$#$ $3so+3$nsi Vi#$min B6 p$1$ m$C % '*6! nm& $1$l$h (8(8-(= 1$n p$1$ m$C *
'-*08! nm& $1$l$h (8=6*, Be+1$s$+$n houm L$m3e+#5Bee+ .$n" men.$#$$n 3$h$/$
in#ensi#$n .$n" 1i#e+us$n oleh l$+u#$n 9$# pen.e+$p 3e+3$n1in" lu+us 1en"$n #e3$l 1$n
onsen#+$si l$+u#$n,
A , 3 , 7
Sehin""$ 1ie#$hui 3$h/$ $3so+3$nsi 'A& 3e+3$1in" lu+us 1en"$n $3so+#ii#$s ' &8
#e3$l ue# '3&8 1$n onsen#+$si '7&, sup$.$ nil$i 3 #e#$p8 m$$ sel$m$ pen"uu+$n
1i"un$$n ue# 1en"$n e#e3$l$n .$n" s$m$, D$l$ houm L$m3e+#5Bee+ #e+1$p$#
3e3e+$p$ pem3$#$s$n .$i#u su$+ 1i"un$$n 1i$n""$p mono+om$#is8 pen.e+$p$n #e+4$1i
1$l$m su$#u oume .$n" mempun.$i pen$mp$n" lu$s .$n" s$m$8 sen.$/$ .$n"
men.e+$p 1$l$m l$u#$n #e+se3u#8 1$n #i1$ #e+4$1i pe+is#i/$ luo+osensi 1$n oso+esensi,
D$n 1i1$p$# pe+s$m$$n :
A p$1$ *6! nm : -=- A %!-8! B ,,,,,,,,, pe+s$m$$n '%&
A p$1$ *6! nm : 0% A -)% B ,, pe+s$m$$n '*&
Kemu1i$n 1iuu+ $3so+3$nsi s$mpel8 #e#$pi $+en$ s$mpel 1$l$m 3en#u #$3le#
m$$ 3e+$# s$#u #$3le# 1i#im3$n" emu1i$n 1i"e+us 1$n 1i3u$# l$+u#$n #e+le3ih 1$hulu,
Den"$n 7$+$ s$mpel 1i#im3$n" %((8( m" 1en"$n ses$m$ .$n" 1il$u$n p$1$ ne+$7$
$n$li#i epe$$n (8% m", menim3$n" ses$m$ .$n" 1im$su1 $1$l$h 1ei$si
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penim3$n"$n #i1$ 3oleh le3ih 1$+i (8% 1$+i 3e+$# 9$# .$n" 1i#im3$n", Sel$n4u#n.$
1il$u$n 1en"$n pel$+u# .$n" s$m$ 1en"$n l$+u#$n s#o 3$u .$i#u $u$1es# 1$l$m l$3u
#$$+ %(8( mL emu1i$n 1is$+in" sup$.$ l$+u#$n 4e+nih8 $+en$ 3il$ #i1$ 4e+nih 3is$
#e+4$1i pen"h$m3u+$n /$+n$ p$1$ s$$# pem3$7$$n $3so+3$nsin.$,
P$1$ pem3$7$$n $3so+3$nsi8 l$+u#$n 1i$m3il *!L 1$n 1i#$m3$h$n $u$1es# $1
%(8( mL 1$l$m l$3u #$$+, Dil$u$n o+ien#$si p$1$ pem3$7$$n m$C % '*6! nm&
1i1$p$# $3so+3$nsi (86-*8 p$1$ m$C * '-*08! nm& 1i1$p$# $3so+3$nsi (8(0*,
P$1$ m$C % '*6! nm&8 $3so+3$nsi +$#$5+$#$ 1$+i e#i"$ +epli$si $1$l$h (86=;G (86%;G
(866%, P$1$ m$C * '-*08! nm& $3so+3$nsi +$#$5+$#$ 1$+i e#i"$ +epli$si $1$l$h
(8(-=!G (8(-(!G (8(--!, Den"$n pe+s$m$$n se3elumn.$ 1$p$# 1ihi#un" onsen#+$si s$mpel,
Konsen#+$si +$#$5+$#$ INH 1$l$m s$mpel .$n" 1i1$p$# $1$l$h =68%(= 3@31$n onsen#+$si
Vi#$min B6 +$#$5+$#$ 1$l$m s$mpel $1$l$h 5-8(0) 3@3, h$sil .$n" 1ipe+oleh Vi#$min B6
1$l$m s$mpel $1$l$h ne"$#i8 m$$ 1imun"in$n 1$l$m s$mpel $1$+ $ein su1$h
3e+u+$n" $#$u 3$h$n su1$h #i1$ $1$, Te#$pi h$sil ini 1$p$# 4u"$ 1imun"in$n $+en$
$1$ 3e3e+$p$ $7#o+ es$l$h$n 1$l$m p+$#ium sepe+#i menim3$n" s$mpel u+$n" #eli#i8
p$1$ s$$# pem3u$#$n l$+u#$n un#u 1i3$7$ $3so+3$nsin.$8 men"$1$nn.$ u+$n" #ep$#8
1$n p$1$ s$$# pen"$m3il$n l$+u#$n 1en"$n mi+opipe# u+$n" 3en$+, P$1$ s$$#
pem3$7$$n $3so+3$nsi semu$ l$+u#$n8 1i"un$$n l$+u#$n 3l$no .$i#u $u$1es# 's$m$
sepe+#i pel$+u#&, K$+en$ 3l$no 3e+un"si un#u mene#+$l$n $#$u men"hil$n"$n$3so+3$nsi 1$+i pel$+u# 1$l$m s$mpel8 sehin""$ .$n" #e+3$7$ p$1$ $l$# $3so+3$n h$n.$
$3so+3$nsi 1$+i s$mpel #$np$ $1$ "$n""u$n 1$+i pel$+u#,
D$+i e$lu$si h$sil 1i1$p$#$n $1$+ INH 1$l$m #$3le# #e+se3u# $1$l$h '=68%(=
*8;6;=& 3@31$n un#u $1$+ Vi$min B6 1$l$m #$3le# $1$l$h '5-8(0) -86--& 3@3,
KESIMPULAN
%& S$mpel men"$n1un" INH 1$n Vi#$min B6 1$p$# 1i#e#$p$n 1$+n.$ 1en"$n me#o1e
pe+s$m$$n simul#$n8 $+en$ INH 1$n Vi#$min B6 mempun.$i "u"us +omoo+,*& H$si ne"$#i p$1$ Vi#$min B6 1$p$# 1i$+#i$n 3$h/$ m$sih #e+3$7$n.$ INH p$1$
pem3$7$$n $+en$ mempun.$i .$n" #i1$ 3e"i#u 4$uh,
-& D$+i h$sil pe+7o3$$n 1ipe+oleh $1$+ 1$l$m s$mpel
INH '=68%(= *8;6;=& 3@3
Vi#$min B '5-8(0) -86--& 3@3,
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DAFTAR PUSTAKA
Anonim, %;=;, Farmakope Indonesia Edisi III, Depes RI, >$$+#$
Au#e+ho 1$n Ko$+, *((*,Identifikasi Obat, ITB, B$n1un", %6!
?$n14$+8 I3nu ?holi3 1$n Rohm$n, *((=,Kimia Farmasi nalisi, Pus#$$ Pel$4$+, 2o".$$+#$, *0;
Ro#h8 He+m$nn, >, 1$n Bl$s7he8 ?o##+ie1, %;)),nalisi Farm asi,U?M, 2o".$$+#$, -6)5-6;
PENETAPAN KADAR TABLET ISONIAID DAN P2RIDOINE MEN??UNAKAN
SPEKTROFOTOMETRI UV DEN?AN METODE SIMULTAN
I, TU>UAN PEROBAAN
ntukmen&etauiarapenetapankadarta'let i#%nia.id dan pyrid%ine
men&&unakan#pektr%C%t%metriu9den&anmet%de#imultan
II, DASAR TEORI;adia#ielektr%ma&netik( yan& mana#inar ultra9i%let
dan#inartampakmerupakan#ala#atunya( dapatdian&&ap#e'a&aiener&i yan&
meram'atdalam'entuk&el%m'an&
Be'erapai#tiladanu'un&andi&unakanuntukmen&&am'arkan&el%m'an&ini
anDan&&el%m'an&merupakanDarak linear dari#uatutitikpada#atu&el%m'an&ketitik
yan& 'er#e'elaanpada&el%m'an& yan& 'erdekatan)I'nuE%li'EinanDardan
;%man(2007+
Biladiin&inkanpen&ukurandua'ua#enyawa#eara'er#ama F
#amapada#pektr%C%t%metri( makadapatdilakukanpada 2 panDan&&el%m'an& yan&
manama#in& F ma#in&k%mp%nentidak#alin&men&&an&&uatau&an&&uandarik%mp%nen
yan& alin palin& keil ua'uakr%m%C%r yan&
'er'edaakanmempunyaikekuatana'#%r'#iaaya yan& 'er'eda pula
pada#atudaerapanDan&&el%m'an& en&ukurandilakukanpadama#in& F
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ma#in&larutanpada 2 panDan&&el%m'an&( #ein&&adiper%le 2
per#amaanu'un&anantaraa'#%r'an#iden&ank%n#entra#ipadaduapanDan&&el%m'an&(
aki'atnyak%n#entra#ima#in& F
ma#in&k%mp%nendapatdiitun&)I'nuE%li'EinanDardan ;%man(2007+
adaper%'aanini'ertuDuanuntukmenetapkankadar INH dalam#ediaan ta'let
yan& men&andun& INH
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di#e'a'kan%lepilantiamil( yan& manadikiraadau'un&annyadena&nkekuran&an
#er%t%nin di %takaki'at meta'%li#m tript%Can yan& dipertin&&i)=e##enden(17+ntuk#uatularutan yan& men&andun& 2 k%mp%nen yan& menyerap( dan y(
#erapandiukurpada 2 panDan&&el%m'an& ,etelitiandantin&&i di
dapatkanden&anmemilipanDan&&el%m'an&dimanapanDan&&el%m'an&pen&ukuranmer
upakanpanDan&&el%m'an&dimana#erapanyamak#imal(
karenaden&anper&e#eran#edikitpadakur9a#erapantidakmenye'a'kanperu'aan#erapa
n yan& terlampauDau)wwwdi&ili'uiaid+
@umlak%mp%nendalamampurandapatmenapai 8
k%mp%nenden&an#yarat#eli#ipanDan&&el%m'an&mak#imumantarak%mp%nen minimal
5 nm @ikaDumlak%mp%nendalam#ampelle'idari 3( makauntukmen&itun&kadar di
&unakan #%Ctware multik%mp%nen yan& terdapatpadaalat#pektr%C%t%metri V
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dilakukanden&anmem'uatkur9au'un&antaraa'#%r'an#iden&anpanDan&&el%m'an&dar
i#uatularutan'akupadak%n#entra#itertentu)I'nuE%li'EinanDardan ;%man(2007+
III, SIFAT BAHAN
Isoni$9i1@INH 'M1 -6 h$l *))&
;umu#m%lekul :Bm :137(1
emeriaan :tidak'erwarna( putitidak'er'au (,ri#tal atau'u'uk ,ri#tal puti
,elarutan : 1:8 air( 1:50 alk%%l( #edikitlarutdalamkl%r%C%m(
#an&at#edikitlarutdalameter
P.+i1oCine @i#$min B6 'M1 -6 h$l %;=)&
;umu#m%lekul :Bm :205(6
emerian :putiprakti#puti(,ri#tal 'u'uk ,ri#tal
,elarutan : 1:5 )air+( 1:115 )alk%%l+( tidaklarutdalameter
Isoni$9i1 'AOA8 p,*!*&
I#%nia.id $am'da Ga !1* 1m S%l9ent
I#%nia.id 265 420 0(01 N Hl
I#%nia.id 263 370 5* tH
I#%nia.id 267 374 0(5 N H2S4I#%nia.id 26 22 0(5 N NaH
P.+i1oCine Hl 'AOA8 p,*!;&yrid%ine Hl $am'da Ga !1* 1m S%l9ent
yrid%ine Hl 288 345 5* tH
yrid%ine Hl 20 425 0(1 N Hl
yrid%ine Hl 324( 254 350( 180 H2
elarutterpili 5*tH
Ren#$n"$3so+3$nsi (8! < %8!
I#%nia.id
0,2
370x10000
1,5
370x10000
5(4 ppm 40(5 ppm
;entan&terpili 5(4< 40(5
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yrid%ine
0,2
345x10000
1,5
345x10000
5(8 ppm 43(5 ppm
;entan&terpili 5(8
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6 J2244ppm
0,16
10=35,90ppm
ara kerDa :
ipetlarutan'akuinduk )30 Kl( 50Kl( 70Kl( 130Kl( 160Kl+
Ga#in& F ma#in&ditam'akanetan%l ad 10 ml( eka'#%r'an#inya
L$+u#$n3$uin1u p.+i1oCine
Konsentrasi :50,4 mg
50ml 1000=1008ppm
ara kerDa:
Tim'an& 50 m& pyrid%ine
$arutkandalamla'utakar 50 ml den&anetan%l ad 50 ml
,%%k ad %m%&en
$aruan'aku :
1 J1008ppm
0,03
10=3,02ppm
2J1008 ppm
0,05
10=5,04 ppm
3J1008ppm
0,07
10=7,06ppm
4J1008ppm
0,1
10=10,08ppm
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5J1008ppm
0,13
10=13,10ppm
6 J
1008ppm0,16
10
=16,13ppm
ara kerDa :
ipetlarutan'akuinduk )30 Kl( 50Kl( 70Kl( 130Kl( 160Kl+
Ga#in& F ma#in&ditam'akanetan%l ad 10 ml( eka'#%r'an#inya
P+ep$+$sis$mpel
ara kerDa :
Tim'anampel#e'anyak 25 m&
Tam'akanetan%l ad 25 ml dalamla'utakar 25 mll
Sarin&den&ankerta#watman
ipet 0(3 ml )300 Kl+ den&anmikr%pipet
Tam'akanetan%l ad 10 ml dalamla'utakar )30 ppm+
Bo3o# +$#$ < +$#$ - #$3le#1,6901
3=0,56 g 563mg
I#%nia.id x
400563=25mg
x=17,76mg
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yrid%ine x
10563=25mg
0(44 m&
Penim3$n"$ns$mpel
Sampel I 0(0268 &
Sampel II 0(026 &
Sampel III 0(0258 &
Pe+hi#un"$nonsen#+$sis$mpel
Sampel I 26,8mg
25ml 1000=1072ppm
1072 0,3ml
10ml 32(16
Sampel II 26,9mg
25ml 1000=1076ppm
1076 0,3ml
10ml 32(28
Sampel III 25,8mg
25ml 1000=1032ppm
1032
0,3ml
10ml 30(6
T$3elpen"$m$#$n3$u Isoni$9i1 'INH&
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,%n#entra#i !'# L 1 I#%nia.id
)265(5+
!'# L2 yrid%ine
)286(5+
!1*1m I#%nia.id !1*
1m yrid%ine
6(73 0(183 0(07 271(2 117(38
11(22 0(31 0(318 284(31 283(42
15(71 0(45 0(204 22(17 12(85
22(44 0(633 0(275 282(0 122(55
2(17 0(826 0(367 283(17 125(81
35(30 0(53 0(432 265(46 120(33
R$#$5+$#$ *=;8)! R$#$5+$#$ %0;8);
Isoni$9i1p$1$
isoni$9i1
C1=0,183
6,7310.000
271(2
C2=0,319
11,22
10.000
284(31
C3=0,459
15,7110.000
22(17
C4=0,633
22,4410.000
282(0
C5=0,826
29,1710.000
283(17
C6=0,953
35,9010.000
265(46
Isoni$9i1p$1$
p.+i1oCine
C1=0,079
6,7310.000
117(38
C2=0,138
11,2210.000
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283(42
C3=0,204
15,7110.000
12(85
C4=0,275
22,4410.000
122(55
C5=0,367
29,1710.000
125(81
C6=0,432
35,9010.000
120(33
T$3elpen"$m$#$n B$u P.+i1oCine
,%n#entra#i !'# L 1 I#%nia.id
)265(5+
!'# L2 yrid%ine
)286(5+
!1*1m I#%nia.id !1*
1m yrid%ine
3(02 0(014 0(057 46(36 188(74
5(04 0(028 0(110 55(55 218(25
7(06 0(060 0(184 84(5 260(62
10(08 0(083 0(238 82(34 236(11
13(10 0(104 0(311 7(3 237(40
16(13 0(153 0(41 4(85 25(76
R$#$5+$#$ =-8;% R$#$5+$#$ *--80)
P.+i1oCine p$1$
isoni$9i1
C1=0,014
3,0210.000
46(36
C2=0,028
5,04 10.000
55(55
C3=0,060
7,0610.000
84(5
C4=0,083
10,0810.000
82(34
C5=0,104
13,10
10.000
7(3
C6=0,153
16,1310.000
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4(85
P.+i1oCinep$1$
p.+i11oCine
C1=0,0573,02
10.000
188(74
C2=0,110
5,0410.000
218(25
C3=0,184
7,0610.000
260(62
C4=
0,238
10,08 10.000
236(11
C5=0,311
13,1010.000
237(40
C6=0,419
16,1310.000
25(76
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T$3elPen"$m$#$ns$mpel
,%n#entra#i !'# L 1I#%nia.id )265(5+ !'# L 1I#%nia.id )286(5+
32(16 0(617 0(2732(28 0(430 0(15
30(6 0(270 0(270
Sampel1:0,617=279,85INH . CINH+73,91PR. CPRx 233,48
0,279=149,89INH .CINH+233,48PR. CPRx73,91
144,05716=65339,378INH .CINH+17256,5068PR
20,62089=11078,3699INH .CINH+17256,5068PR
123,43627=54261,0081INH . CINH
CINH=2,275103 10.000=22,75ppm
CPR=
Sampel2:0,430=279,85INH . CINH+73,91PR. CPRx233,48
0,195=149,89INH .CINH+233,48PR. CPRx73,91
100,3964=65339,378INH . CINH+17256,5068PR
14,41245=11078,3699INH . CINH+17256,5068PR
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85,98395=54261,0081INH . CINH
CINH=1,585103
10.000=15,85ppm
CPR=
Sampel3:0,600=279,85INH . CINH+73,91PR .CPRx 233,48
0,270=149,89INH .CINH+233,48PR . CPRx73,91
140,088=65339,378INH .CINH+17256,5068PR
19,957=11078,3699INH . CINH+17256,5068PR
120,1323=54261,0081INH . CINH
CINH=2,213103
10.000=22,13ppm
CPR=
Pe+hi#un"$n$1$+ isoni$9i1
Sampel 1 22,75
32,16x100
70(74 *
Sampel 2 15,85
32,28x100
4(10 *
7/21/2019 spektrofotometri uv zat campuran
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Sampel 3 22,13
30,96x100
71(47 *
Pe+hi#un"$n3e+$#
Sampel 1 70(47 * 563 36( 75
Sampel 2 4( 10 * 563 276(43
Sampel 3 71(47 * 563 402(37
Pe+hi#un"$n 01
,adar )*+ ;ata
7/21/2019 spektrofotometri uv zat campuran
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tidakadapartikelk%l%idapala&i#u#pen#i(
karenaapa'ila#ampeltidakDerniataudikatakanterdapat#enyawa lain yan&
tidaklarutmakaa#ilpem'aaana'#%r'an#imenDaditidaktepat
adapenetapankadarkap#ulkl%ramCenik%linipraktikanmelakukanprepara#i#ampelden&anmet%deCil
tra#iatau di di#arin&men&&unkankerta##arin&
adapraktikuminikel%mp%k kami mendapatkana#ilDumla i#%nia.id 3(56 m& ta'let
#edan&kankadar#e'enarnyaadala 400 m&ta'let #edan&kankadarpyrid%k#inetidakter'aa
,e#alaandalampenetapankadarinidi#e'a'kankarenakelarutanpyrid%yne#an&at'e#ardankadarpy
rid%yne yan& terlalukeilyaitu 10 m&ta'let #edankankadar i#%nia.id 400 m&ta'let
dankearutannyayaitu 1:115 dalametan%ldankelarutan i#%nia.id 1:50 dalametan%l
!ki'atdariper'edaankelarutandankadar yan& #i&niCikaninimakaada#eDumlapyrid%yne yan&
tidakterlarut#empurna#elainituDu&ama#iada yan& tertin&&aldalamla'uukurmaupundalam pr%#e#
penyarin&an#ein&&aketikadilakukanpenetapankadarmakatidakter'aa
apatdikatakanDu&a#emakinkeilkadar%'at yan&
akanditentukanmakaakan#emakin'e#arper#enke#alaan yan& didapat
KESIMPULAN
,adar pyrid%ine yan& didapatadala 3(56 m& ta'let
DAFTAR PUSTAKA
!n%nim 17 =armak%pe Ind%ne#ia di#i III epartemen ,e#eatan;epu'likInd%ne#ia :
@akarta
;%man( !'dul( I'nuEandDar 2007Kimia FarmasiAnalisis u#takaBelaDar : %&yakarta 2008
Kimia FarmasiAnalisis.u#takaelaDar : %&yakarta
=e##enden( ;alp @ dan @%an S =e##enden( 17( OKimia OrganikP( Dilid 1 edi#iketi&a(
terDemaan%le : !l%y#iu# H ( ener'itrlan&&a( @akarta
Gartindale )200+ Martindale The Complete Drug Reference. < 36t diti%n
edarmaeutial re##
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