64
64
Lampiran 2. Surat Keterangan Telah Melakukan Penelitian dari Bagian
Biologi Farmasi Fakultas Farmasi Universitas Wahid Hasyim
Semarang
65
65
Lampiran 3. Surat Keterangan Telah Melakukan Penelitian dari Bagian
Teknologi Farmasi Fakultas Farmasi Universitas Wahid
Hasyim Semarang
66
66
Lampiran 4. Surat Keterangan Telah Melakukan Penelitian dari Bagian
Farmakologi dan Farmasi Klinik Fakultas Farmasi
Universitas Wahid Hasyim Semarang
67
67
Lampiran 5. Surat Keterangan Telah Melakukan Pembelian Tikus Putih
(Rattus Norvegicus) Galur Wistar usia 2-3 bulan, Jenis
Kelamin Jantan Sebanyak 25 Ekor.
68
68
Lampiran 6. Surat Keterangan Ethical Clearance Fakultas Kedokteran
Universitas Islam Sultan Agung
71
71
Lampiran 8. Perhitungan Rendemen Ekstrak Etanol Daun Jambu Biji
Diketahui : bobot simplisia daun jambu biji = 2000 gram
Bobot ekstrak kental = 515 gram
Ditanya : randemen daun jambu biji ?
Jawab :
72
72
Lampiran 9. Perhitungan Nilai Bahan Pembuatan Gel EEDJB
Bahan Konsentrasi sediaan (b/b)
F1 F2 F3 F4
Ekstrak daun jambu biji (g)
Karbopol 934 (g)
Gliserin (mL)
Metil paraben (g)
Propil paraben (g)
Triethanolamine (TEA) (mL)
Akuades (mL) ad
3
1
2
0,2
0,2
1,25
100
5
1
2
0,2
0,2
1,25
100
7
1
2
0,2
0,2
1,25
100
-
1
2
0,2
0,2
1,25
100
Formula 1
Ekstrak daun jambu biji (g)
Karbopol 934 (g)
Gliserin (mL)
Metil paraben (g)
Propil paraben (g)
Triethanolamine (mL)
Akuades ad 100 (mL)
3
1
2
0,2
0,2
1,25 +
7,65
100-7,65 = 92,35 ≈ 92 mL
Formula 2
Ekstrak daun jambu biji (g)
Karbopol 934 (g)
Gliserin (mL)
Metil paraben (g)
Propil paraben (g)
Triethanolamine (mL)
Akuades ad 100 (mL)
5
1
2
0,2
0,2
1,25 +
9,65
100-9,65 = 90,35 ≈ 90 mL
Formula 3
Ekstrak daun jambu biji (g)
Karbopol 934 (g)
Gliserin (mL)
Metil paraben (g)
Propil paraben (g)
Triethanolamine (mL)
Akuades ad 100 (mL)
7
1
2
0,2
0,2
1,25 +
11,65
100-11,65 = 88,35 ≈ 88 mL
73
73
Lampiran 9. Lanjutan
Formula 4
Ekstrak daun jambu biji (g)
Karbopol 934 (g)
Gliserin (mL)
Metil paraben (g)
Propil paraben (g)
Triethanolamine (mL)
Akuades ad 100 (mL)
-
1
2
0,2
0,2
1,25 +
4,25
100-4,25 = 95,75 ≈ 96 mL
74
74
Lampiran 10. Hasil Pengujian Sifat Fisika dan Kimia Sediaan Gel EEDJB
1. Hasil Pengukuran Viskositas
Replikasi Viskositas Gel EEDJB (dPa.S)
F1 F2 F3 F4
1 200 200 200 150
2 200 200 200 150
3 200 200 200 150
Rata – Rata 200 200 200 200
Keterangan :
Formula 1 : Kelompok 1 gel ekstrak etanol daun jambu biji 3%
Formula 2 : Kelompok 2 gel ekstrak etanol daun jambu biji 5%
Formula 3 : Kelompok 3 gel ekstrak etanol daun jambu biji 7%
Formula 4 : Kelompok kontrol negatif basis gel karbopol
2. Hasil Pengukuran Daya Sebar
Formula Pengukuran Diameter Daya Sebar (cm) dengan Beban Diameter
Rata – Rata (cm) Kaca 50 100 150 200 250 300
F1
R1 3,2 3,3 3,4 3,5 3,6 3,7 3,8 3,50
R2 3,2 3,3 3,4 3,5 3,6 3,6 3,7 3,47
R3 3,3 3,4 3,6 3,7 3,8 3,9 4,0 3,67
Rata – Rata 3,54
F2
R1 3,2 3,3 3,4 3,5 3,6 3,7 3,8 3,50
R2 3,1 3,2 3,3 3,4 3,5 3,6 3,6 3,38
R3 3,1 3,2 3,3 3,4 3,5 3,6 3,7 3,40
Rata – Rata 3,43
F3
R1 3,0 3,1 3,2 3,3 3,4 3,5 3,5 3,28
R2 3,1 3,2 3,3 3,4 3,5 3,6 3,6 3,38
R3 3,0 3,1 3,2 3,3 3,4 3,5 3,6 3,30
Rata – Rata 3,32
F4
R1 4,4 4,5 4,6 4,7 4,8 4,9 5,0 4,70
R2 4,6 4,7 4,8 4,9 5,0 5,0 5,1 4,87
R3 4,5 4,6 4,7 4,8 4,9 5,0 5,1 5,23
Rata – Rata 4,93
Keterangan :
Formula 1 : Kelompok 1 gel ekstrak etanol daun jambu biji 3%
Formula 2 : Kelompok 2 gel ekstrak etanol daun jambu biji 5%
Formula 3 : Kelompok 3 gel ekstrak etanol daun jambu biji 7%
Formula 4 : Kelompok kontrol negatif basis gel karbopol
75
75
Lampiran 10. Lanjutan
3. Hasil Pengukuran Daya Lekat
Replikasi Daya Lekat Gel EEDJB (detik)
F1 F2 F3 F4
1 2,36 2,85 3,55 1,83
2 2,33 2,88 3,43 1,57
3 2,41 2,55 3,30 1,56
Rata – Rata 2,37 2,76 3,42 1,66
Keterangan :
Formula 1 : Kelompok 1 gel ekstrak etanol daun jambu biji 3%
Formula 2 : Kelompok 2 gel ekstrak etanol daun jambu biji 5%
Formula 3 : Kelompok 3 gel ekstrak etanol daun jambu biji 7%
Formula 4 : Kelompok kontrol negatif basis gel karbopol
4. Hasil Pengukuran pH
Replikasi Derajat Keasaman Gel EEDJB (pH)
F1 F2 F3 F4
1 6,99 6,66 6.58 7,15
2 6,99 6,66 6,58 7,15 3 6,99 6,66 6,58 7,15
Rata – Rata 6,99 6,66 6,58 7,15
Keterangan :
Formula 1 : Kelompok 1 gel ekstrak etanol daun jambu biji 3%
Formula 2 : Kelompok 2 gel ekstrak etanol daun jambu biji 5%
Formula 3 : Kelompok 3 gel ekstrak etanol daun jambu biji 7%
Formula 4 : Kelompok kontrol negatif basis gel karbopol
76
76
Lampiran 11. Perhitungan Persentase Penyembuhan Luka Bakar
Perhitungan Persentase Penyembuhan Luka Bakar
Px = dx(1)2-dx(17)
2 X 100%
dx(1)2
Keterangan :
Px = persentase penyembuhan luka bakar pada hari ke 17
dx(1) = diatemer luka bakar hari pertama
dx(17) = diameter luka bakar hari ke 17
K1 EEDJB 3%
Tikus 1 Tikus 2 Tikus 3
Px = 22-0,846
2 X 100 %
22
= 4-0,7157 X 100%
4
= 82,11 %
Px = 22-0,812
2 X 100 %
22
= 4-0,6593 X 100%
4
= 83,51 %
Px = 22-0,861
2 X 100 %
22
= 4-0,7413 X 100%
4
= 81,47 %
Tikus 4 Tikus 5
Px = 22-0,911
2 X 100 %
22
= 4-0,8299 X 100%
4
= 79,25 %
Px = 22-0,961
2 X 100 %
22
= 4-0,9235 X 100%
4
= 79,91 %
K2 EEDJB 5%
Tikus 1 Tikus 2 Tikus 3
Px = 22-0,463
2 X 100 %
22
= 4-0,2144 X 100%
4
= 94,64 %
Px = 22-0,47
2 X 100 %
22
= 4-0,2209 X 100%
4
= 94,48 %
Px = 22-0,44
2 X 100 %
22
= 4-0,1936 X 100%
4
= 95,16 %
Tikus 4 Tikus 5
Px = 22-0,512
2 X 100 %
22
= 4-0,2621 X 100%
4
= 93,45 %
Px = 22-0,709
2 X 100 %
22
= 4-0,5027 X 100%
4
= 87,43 %
77
77
Lampiran 11. Lanjutan
K3 EEDJB 7%
Tikus 1 Tikus 2 Tikus 3
Px = 22-0,237
2 X 100 %
22
= 4-0,0562 X 100%
4
= 98,59 %
Px = 22-0,287
2 X 100 %
22
= 4-0,0824 X 100%
4
= 97,94 %
Px = 22-0
2 X 100 %
22
= 4-0 X 100%
4
= 100 %
Tikus 4 Tikus 5
Px = 22-0
2 X 100 %
22
= 4-0 X 100%
4
= 100 %
Px = 22-0
2 X 100 %
22
= 4-0 X 100%
4
= 100 %
K- Basis Gel Karbopol
Tikus 1 Tikus 2 Tikus 3
Px = 22-1,638
2 X 100 %
22
= 4-2,6830 X 100%
4
= 32,92 %
Px = 22-1,682
2 X 100 %
22
= 4-2,8291 X 100%
4
= 29,27 %
Px = 22-1,595
2 X 100 %
22
= 4-2,5440 X 100%
4
= 36,4 %
Tikus 4 Tikus 5
Px = 22-1,566
2 X 100 %
22
= 4-2,4523 X 100%
4
= 38,69 %
Px = 22-1,565
2 X 100 %
22
= 4-2,4492 X 100%
4
= 38,77 %
78
78
Lampiran 11. Lanjutan
K+ Bioplacenton
Tikus 1 Tikus 2 Tikus 3
Px = 22-0
2 X 100 %
22
= 4-0 X 100%
4
= 100 %
Px = 22-0
2 X 100 %
22
= 4-0 X 100%
4
= 100 %
Px = 22-0,165
2 X 100 %
22
= 4-0,0272 X 100%
4
= 99,32 %
Tikus 4 Tikus 5
Px = 22-0,467
2 X 100 %
22
= 4-0,2181 X 100%
4
= 94,55 %
Px = 22-0,535
2 X 100 %
22
= 4-0,2862 X 100%
4
= 92,84 %
79
79
Lampiran 12. Hasil Uji Normalitas, Kruskall Walis, dan Mann Whitney
Viskositas
1. Uji Normalitas
Tests of Normality
Kolmogorov-Smirnov
a Shapiro-Wilk
Statistic Df Sig. Statistic df Sig.
Viskositas (dPa.S) ,460 12 ,000 ,552 12 ,000
a. Lilliefors Significance Correction
Uji Homogenitas
Test of Homogeneity of Variances
Viskositas (dPa.S)
Levene Statistic df1 df2 Sig.
. 3 . .
2. Uji Kruskall Walis
Ranks
Formula N Mean Rank
Viskositas (dPa.S) K1 Gel EEDJB 3% 3 8,00
K2 Gel EEDJB 5% 3 8,00
K3 Gel EEDJB 7% 3 8,00
K- Basis Gel Karbopol 3 2,00
Total 12
Test Statisticsa,b
Viskositas (dPa.S)
Chi-Square 11,000
Df 3
Asymp. Sig. ,012
a. Kruskal Wallis Test
b. Grouping Variable: Formula
80
80
Lampiran 12. Lanjutan
3. Uji Mann Whitney
Ranks
Formula N Mean Rank Sum of Ranks
Viskositas (dPa.S) K1 Gel EEDJB 3% 3 3,50 10,50
K2 Gel EEDJB 5% 3 3,50 10,50
Total 6
Test Statisticsb
Viskositas (dPa.S)
Mann-Whitney U 4,500
Wilcoxon W 10,500
Z ,000
Asymp. Sig. (2-tailed) 1,000
Exact Sig. [2*(1-tailed Sig.)] 1,000a
a. Not corrected for ties.
b. Grouping Variable: Formula
Ranks
Formula N Mean Rank Sum of Ranks
Viskositas (dPa.S) K1 Gel EEDJB 3% 3 3,50 10,50
K3 Gel EEDJB 7% 3 3,50 10,50
Total 6
Test Statisticsb
Viskositas (dPa.S)
Mann-Whitney U 4,500
Wilcoxon W 10,500
Z ,000
Asymp. Sig. (2-tailed) 1,000
Exact Sig. [2*(1-tailed Sig.)] 1,000a
a. Not corrected for ties.
b. Grouping Variable: Formula
81
81
Lampiran 12. Lanjutan
Ranks
Formula N Mean Rank Sum of Ranks
Viskositas (dPa.S) K1 Gel EEDJB 3% 3 5,00 15,00
K- Basis Gel Karbopol 3 2,00 6,00
Total 6
Test Statisticsb
Viskositas (dPa.S)
Mann-Whitney U ,000
Wilcoxon W 6,000
Z -2,236
Asymp. Sig. (2-tailed) ,025
Exact Sig. [2*(1-tailed Sig.)] ,100a
a. Not corrected for ties.
b. Grouping Variable: Formula
Ranks
Formula N Mean Rank Sum of Ranks
Viskositas (dPa.S) K2 Gel EEDJB 5% 3 3,50 10,50
K3 Gel EEDJB 7% 3 3,50 10,50
Total 6
Test Statisticsb
Viskositas (dPa.S)
Mann-Whitney U 4,500
Wilcoxon W 10,500
Z ,000
Asymp. Sig. (2-tailed) 1,000
Exact Sig. [2*(1-tailed Sig.)] 1,000a
a. Not corrected for ties.
b. Grouping Variable: Formula
82
82
Lampiran 12. Lanjutan
Ranks
Formula N Mean Rank Sum of Ranks
Viskositas (dPa.S) K2 Gel EEDJB 5% 3 5,00 15,00
K- Basis Gel Karbopol 3 2,00 6,00
Total 6
Test Statisticsb
Viskositas (dPa.S)
Mann-Whitney U ,000
Wilcoxon W 6,000
Z -2,236
Asymp. Sig. (2-tailed) ,025
Exact Sig. [2*(1-tailed Sig.)] ,100a
a. Not corrected for ties.
b. Grouping Variable: Formula
Ranks
Formula N Mean Rank Sum of Ranks
Viskositas (dPa.S) K3 Gel EEDJB 7% 3 5,00 15,00
K- Basis Gel Karbopol 3 2,00 6,00
Total 6
Test Statisticsb
Viskositas (dPa.S)
Mann-Whitney U ,000
Wilcoxon W 6,000
Z -2,236
Asymp. Sig. (2-tailed) ,025
Exact Sig. [2*(1-tailed Sig.)] ,100a
a. Not corrected for ties.
b. Grouping Variable: Formula
83
83
Lampiran 13. Hasil Uji Normalitas, Homogenitas, One Way Anova, dan
LSD Daya Sebar
1. Uji Normalitas
Tests of Normality
Formula
Kolmogorov-Smirnova Shapiro-Wilk
Statistic df Sig. Statistic df Sig.
Diameter Daya Sebar (cm) K1 Gel EEDJB 3% ,334 3 . ,860 3 ,266
K2 Gel EEDJB 5% ,328 3 . ,871 3 ,298
K3 Gel EEDJB 7% ,314 3 . ,893 3 ,363
K- Basis Gel Karbopol ,259 3 . ,959 3 ,610
a. Lilliefors Significance Correction
2. Uji Homogenitas
Test of Homogeneity of Variances
Diameter Daya Sebar (cm)
Levene Statistic df1 df2 Sig.
3,729 3 8 ,061
3. Uji One Way Anova
ANOVA
Diameter Daya Sebar (cm)
Sum of Squares df Mean Square F Sig.
Between Groups 5,155 3 1,718 74,868 ,000
Within Groups ,184 8 ,023
Total 5,338 11
84
Lampiran 13. Lanjutan
4. Uji LSD
Multiple Comparisons
Diameter Daya Sebar (cm)
LSD
(I) Formula (J) Formula
Mean
Difference (I-J) Std. Error Sig.
95% Confidence Interval
Lower Bound Upper Bound
K1 Gel EEDJB 3% K2 Gel EEDJB 5% ,12000 ,12369 ,360 -,1652 ,4052
K3 Gel EEDJB 7% ,22667 ,12369 ,104 -,0586 ,5119
K- Basis Gel Karbopol -1,38667* ,12369 ,000 -1,6719 -1,1014
K2 Gel EEDJB 5% K1 Gel EEDJB 3% -,12000 ,12369 ,360 -,4052 ,1652
K3 Gel EEDJB 7% ,10667 ,12369 ,414 -,1786 ,3919
K- Basis Gel Karbopol -1,50667* ,12369 ,000 -1,7919 -1,2214
K3 Gel EEDJB 7% K1 Gel EEDJB 3% -,22667 ,12369 ,104 -,5119 ,0586
K2 Gel EEDJB 5% -,10667 ,12369 ,414 -,3919 ,1786
K- Basis Gel Karbopol -1,61333* ,12369 ,000 -1,8986 -1,3281
K- Basis Gel Karbopol K1 Gel EEDJB 3% 1,38667* ,12369 ,000 1,1014 1,6719
K2 Gel EEDJB 5% 1,50667* ,12369 ,000 1,2214 1,7919
K3 Gel EEDJB 7% 1,61333* ,12369 ,000 1,3281 1,8986
*. The mean difference is significant at the 0.05 level.
85
85
Lampiran 14. Hasil Uji Normalitas, Homogenitas, One Way Anova, dan
LSD Daya Lekat
1. Uji Normalitas
Tests of Normality
Formula
Kolmogorov-Smirnova Shapiro-Wilk
Statistic Df Sig. Statistic df Sig.
Daya Lekat (detik) K1 Gel EEDJB 3% ,232 3 . ,980 3 ,726
K2 Gel EEDJB 5% ,356 3 . ,818 3 ,157
K3 Gel EEDJB 7% ,178 3 . ,999 3 ,956
K- Basis Gel Karbopol ,374 3 . ,778 3 ,062
a. Lilliefors Significance Correction
2. Uji Homogenitas
Test of Homogeneity of Variances
Daya Lekat (detik)
Levene Statistic df1 df2 Sig.
2,366 3 8 ,147
3. Uji One Way Anova
ANOVA
Daya Lekat (detik)
Sum of Squares Df Mean Square F Sig.
Between Groups 4,951 3 1,650 89,203 ,000
Within Groups ,148 8 ,019
Total 5,099 11
86
Lampiran 14. Lanjutan
4. Uji LSD
Multiple Comparisons
Daya Lekat (detik)
LSD
(I) Formula (J) Formula
Mean
Difference (I-J) Std. Error Sig.
95% Confidence Interval
Lower Bound Upper Bound
K1 Gel EEDJB 3% K2 Gel EEDJB 5% -,39333* ,11106 ,008 -,6494 -,1372
K3 Gel EEDJB 7% -1,06000* ,11106 ,000 -1,3161 -,8039
K- Basis Gel Karbopol ,71333* ,11106 ,000 ,4572 ,9694
K2 Gel EEDJB 5% K1 Gel EEDJB 3% ,39333* ,11106 ,008 ,1372 ,6494
K3 Gel EEDJB 7% -,66667* ,11106 ,000 -,9228 -,4106
K- Basis Gel Karbopol 1,10667* ,11106 ,000 ,8506 1,3628
K3 Gel EEDJB 7% K1 Gel EEDJB 3% 1,06000* ,11106 ,000 ,8039 1,3161
K2 Gel EEDJB 5% ,66667* ,11106 ,000 ,4106 ,9228
K- Basis Gel Karbopol 1,77333* ,11106 ,000 1,5172 2,0294
K- Basis Gel Karbopol K1 Gel EEDJB 3% -,71333* ,11106 ,000 -,9694 -,4572
K2 Gel EEDJB 5% -1,10667* ,11106 ,000 -1,3628 -,8506
K3 Gel EEDJB 7% -1,77333* ,11106 ,000 -2,0294 -1,5172
*. The mean difference is significant at the 0.05 level.
87
87
Lampiran 15. Hasil Uji Normalitas, Kruskall Walis, dan Mann Whitney pH
1. Uji Normalitas
Tests of Normality
Kolmogorov-Smirnov
a Shapiro-Wilk
Statistic df Sig. Statistic df Sig.
pH ,276 12 ,012 ,809 12 ,012
a. Lilliefors Significance Correction
Uji Homogenitas
Test of Homogeneity of Variances
pH
Levene Statistic df1 df2 Sig.
. 3 . .
2. Uji Kruskall Walis
Ranks
Formula N Mean Rank
pH K1 Gel EEDJB 3% 3 8,00
K2 Gel EEDJB 5% 3 5,00
K3 Gel EEDJB 7% 3 2,00
K- Basis Gel Karbopol 3 11,00
Total 12
Test Statisticsa,b
pH
Chi-Square 11,000
df 3
Asymp. Sig. ,012
a. Kruskal Wallis Test
b. Grouping Variable:
Formula
88
88
Lampiran 15. Lanjutan
3. Uji Mann Whitney
Ranks
Formula N Mean Rank Sum of Ranks
pH K1 Gel EEDJB 3% 3 5,00 15,00
K2 Gel EEDJB 5% 3 2,00 6,00
Total 6
Test Statisticsb
pH
Mann-Whitney U ,000
Wilcoxon W 6,000
Z -2,236
Asymp. Sig. (2-tailed) ,025
Exact Sig. [2*(1-tailed Sig.)] ,100a
a. Not corrected for ties.
b. Grouping Variable: Formula
Ranks
Formula N Mean Rank Sum of Ranks
pH K1 Gel EEDJB 3% 3 5,00 15,00
K3 Gel EEDJB 7% 3 2,00 6,00
Total 6
Test Statisticsb
pH
Mann-Whitney U ,000
Wilcoxon W 6,000
Z -2,236
Asymp. Sig. (2-tailed) ,025
Exact Sig. [2*(1-tailed Sig.)] ,100a
a. Not corrected for ties.
b. Grouping Variable: Formula
89
89
Lampiran 15. Lanjutan
Ranks
Formula N Mean Rank Sum of Ranks
pH K1 Gel EEDJB 3% 3 2,00 6,00
K- Basis Gel Karbopol 3 5,00 15,00
Total 6
Test Statisticsb
pH
Mann-Whitney U ,000
Wilcoxon W 6,000
Z -2,236
Asymp. Sig. (2-tailed) ,025
Exact Sig. [2*(1-tailed Sig.)] ,100a
a. Not corrected for ties.
b. Grouping Variable: Formula
Ranks
Formula N Mean Rank Sum of Ranks
pH K2 Gel EEDJB 5% 3 5,00 15,00
K3 Gel EEDJB 7% 3 2,00 6,00
Total 6
Test Statisticsb
pH
Mann-Whitney U ,000
Wilcoxon W 6,000
Z -2,236
Asymp. Sig. (2-tailed) ,025
Exact Sig. [2*(1-tailed Sig.)] ,100a
a. Not corrected for ties.
b. Grouping Variable: Formula
90
90
Lampiran 15. Lanjutan
Ranks
Formula N Mean Rank Sum of Ranks
pH K2 Gel EEDJB 5% 3 2,00 6,00
K- Basis Gel Karbopol 3 5,00 15,00
Total 6
Test Statisticsb
pH
Mann-Whitney U ,000
Wilcoxon W 6,000
Z -2,236
Asymp. Sig. (2-tailed) ,025
Exact Sig. [2*(1-tailed Sig.)] ,100a
a. Not corrected for ties.
b. Grouping Variable: Formula
Ranks
Formula N Mean Rank Sum of Ranks
pH K3 Gel EEDJB 7% 3 2,00 6,00
K- Basis Gel Karbopol 3 5,00 15,00
Total 6
Test Statisticsb
pH
Mann-Whitney U ,000
Wilcoxon W 6,000
Z -2,236
Asymp. Sig. (2-tailed) ,025
Exact Sig. [2*(1-tailed Sig.)] ,100a
a. Not corrected for ties.
b. Grouping Variable: Formula
91
91
Lampiran 16. Hasil Uji Normalitas, Homogenitas, Kruskall Walis, dan Mann
Whitney Persentase Penyembuhan Luka Bakar
1. Uji Normalitas
2. Uji Homogenitas
Test of Homogeneity of Variance
Levene Statistic df1 df2 Sig.
Persentase Penyembuhan
Luka Bakar
Based on Mean 2,359 4 20 ,088
Based on Median ,699 4 20 ,602
Based on Median and with
adjusted df
,699 4 15,3
56
,604
Based on trimmed mean 2,080 4 20 ,122
3. Uji Kruskall Walis
Ranks
Kelompok Hewan Uji N Mean Rank
Persentase Penyembuhan
Luka Bakar
K1 Gel EEDJB 3% 5 8,00
K2 Gel EEDJB 5% 5 14,20
K3 Gel EEDJB 7% 5 21,20
K- Basis Gel Karbopol 5 3,00
K+ Bioplacenton 5 18,60
Total 25
Tests of Normality
Kelompok Hewan Uji
Kolmogorov-Smirnova Shapiro-Wilk
Statistic Df Sig. Statistic df Sig.
Persentase Penyembuhan
Luka Bakar
K1 Gel EEDJB 3% ,224 5 ,200* ,957 5 ,787
K2 Gel EEDJB 5% ,352 5 ,042 ,722 5 ,016
K3 Gel EEDJB 7% ,361 5 ,032 ,764 5 ,040
K- Basis Gel Karbopol ,215 5 ,200* ,890 5 ,358
K+ Bioplacenton ,320 5 ,104 ,800 5 ,081
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
92
92
Lampiran 16. Lanjutan
Test Statisticsa,b
Persentase Penyembuhan
Luka Bakar
Chi-Square 20,934
Df 4
Asymp. Sig. ,000
a. Kruskal Wallis Test
b. Grouping Variable: Kelompok Hewan Uji
4. Uji Mann Whitney
Ranks
Kelompok Hewan Uji N Mean Rank Sum of Ranks
Persentase Penyembuhan
Luka Bakar
K1 Gel EEDJB 3% 5 3,00 15,00
K2 Gel EEDJB 5% 5 8,00 40,00
Total 10
Test Statisticsb
Persentase Penyembuhan
Luka Bakar
Mann-Whitney U ,000
Wilcoxon W 15,000
Z -2,611
Asymp. Sig. (2-tailed) ,009
Exact Sig. [2*(1-tailed Sig.)] ,008a
a. Not corrected for ties.
b. Grouping Variable: Kelompok Hewan Uji
Ranks
Kelompok Hewan Uji N Mean Rank Sum of Ranks
Persentase Penyembuhan
Luka Bakar
K1 Gel EEDJB 3% 5 3,00 15,00
K3 Gel EEDJB 7% 5 8,00 40,00
Total 10
93
93
Lampiran 16. Lanjutan
Test Statisticsb
Persentase Penyembuhan
Luka Bakar
Mann-Whitney U ,000
Wilcoxon W 15,000
Z -2,643
Asymp. Sig. (2-tailed) ,008
Exact Sig. [2*(1-tailed Sig.)] ,008a
a. Not corrected for ties.
b. Grouping Variable: Kelompok Hewan Uji
Ranks
Kelompok Hewan Uji N Mean Rank Sum of Ranks
Persentase Penyembuhan
Luka Bakar
K1 Gel EEDJB 3% 5 8,00 40,00
K- Basis Gel Karbopol 5 3,00 15,00
Total 10
Test Statisticsb
Persentase Penyembuhan
Luka Bakar
Mann-Whitney U ,000
Wilcoxon W 15,000
Z -2,611
Asymp. Sig. (2-tailed) ,009
Exact Sig. [2*(1-tailed Sig.)] ,008a
a. Not corrected for ties.
b. Grouping Variable: Kelompok Hewan Uji
Ranks
Kelompok Hewan Uji N Mean Rank Sum of Ranks
Persentase Penyembuhan
Luka Bakar
K1 Gel EEDJB 3% 5 3,00 15,00
K+ Bioplacenton 5 8,00 40,00
Total 10
94
94
Lampiran 16. Lanjutan
Test Statisticsb
Persentase Penyembuhan
Luka Bakar
Mann-Whitney U ,000
Wilcoxon W 15,000
Z -2,619
Asymp. Sig. (2-tailed) ,009
Exact Sig. [2*(1-tailed Sig.)] ,008a
a. Not corrected for ties.
b. Grouping Variable: Kelompok Hewan Uji
Ranks
Kelompok Hewan Uji N Mean Rank Sum of Ranks
Persentase Penyembuhan
Luka Bakar
K2 Gel EEDJB 5% 5 3,00 15,00
K3 Gel EEDJB 7% 5 8,00 40,00
Total 10
Test Statisticsb
Persentase Penyembuhan
Luka Bakar
Mann-Whitney U ,000
Wilcoxon W 15,000
Z -2,643
Asymp. Sig. (2-tailed) ,008
Exact Sig. [2*(1-tailed Sig.)] ,008a
a. Not corrected for ties.
b. Grouping Variable: Kelompok Hewan Uji
Ranks
Kelompok Hewan Uji N Mean Rank Sum of Ranks
Persentase Penyembuhan
Luka Bakar
K2 Gel EEDJB 5% 5 8,00 40,00
K- Basis Gel Karbopol 5 3,00 15,00
Total 10
95
95
Lampiran 16. Lanjutan
Test Statisticsb
Persentase Penyembuhan
Luka Bakar
Mann-Whitney U ,000
Wilcoxon W 15,000
Z -2,611
Asymp. Sig. (2-tailed) ,009
Exact Sig. [2*(1-tailed Sig.)] ,008a
a. Not corrected for ties.
b. Grouping Variable: Kelompok Hewan Uji
Ranks
Kelompok Hewan Uji N Mean Rank Sum of Ranks
Persentase Penyembuhan
Luka Bakar
K2 Gel EEDJB 5% 5 4,20 21,00
K+ Bioplacenton 5 6,80 34,00
Total 10
Test Statisticsb
Persentase Penyembuhan
Luka Bakar
Mann-Whitney U 6,000
Wilcoxon W 21,000
Z -1,362
Asymp. Sig. (2-tailed) ,173
Exact Sig. [2*(1-tailed Sig.)] ,222a
a. Not corrected for ties.
b. Grouping Variable: Kelompok Hewan Uji
Ranks
Kelompok Hewan Uji N Mean Rank Sum of Ranks
Persentase Penyembuhan
Luka Bakar
K3 Gel EEDJB 7% 5 8,00 40,00
K- Basis Gel Karbopol 5 3,00 15,00
Total 10
96
96
Lampiran 16. Lanjutan
Test Statisticsb
Persentase Penyembuhan
Luka Bakar
Mann-Whitney U ,000
Wilcoxon W 15,000
Z -2,643
Asymp. Sig. (2-tailed) ,008
Exact Sig. [2*(1-tailed Sig.)] ,008a
a. Not corrected for ties.
b. Grouping Variable: Kelompok Hewan Uji
Ranks
Kelompok Hewan Uji N Mean Rank Sum of Ranks
Persentase Penyembuhan
Luka Bakar
K3 Gel EEDJB 7% 5 6,20 31,00
K+ Bioplacenton 5 4,80 24,00
Total 10
Test Statisticsb
Persentase Penyembuhan
Luka Bakar
Mann-Whitney U 9,000
Wilcoxon W 24,000
Z -,780
Asymp. Sig. (2-tailed) ,435
Exact Sig. [2*(1-tailed Sig.)] ,548a
a. Not corrected for ties.
b. Grouping Variable: Kelompok Hewan Uji
Ranks
Kelompok Hewan Uji N Mean Rank Sum of Ranks
Persentase Penyembuhan
Luka Bakar
K- Basis Gel Karbopol 5 3,00 15,00
K+ Bioplacenton 5 8,00 40,00
Total 10
97
97
Lampiran 16. Lanjutan
Test Statisticsb
Persentase Penyembuhan
Luka Bakar
Mann-Whitney U ,000
Wilcoxon W 15,000
Z -2,619
Asymp. Sig. (2-tailed) ,009
Exact Sig. [2*(1-tailed Sig.)] ,008a
a. Not corrected for ties.
b. Grouping Variable: Kelompok Hewan Uji
98
98
Lampiran 17. Dokumentasi Penelitian
Tempat Pengambilan Simplisia Proses Sortasi Basah
Proses Penimbangan Proses Pengeringan
Proses Sortasi Kering Pengecekan Kadar Air
99
99
Lampiran 17. Lanjutan
Proses Penyerbukan Simplisia Serbuk Daun Jambu Biji
Penimbangan Serbuk Proses Maserasi
Proses Penyaringan Maserasi Hasil Maserat
100
100
Lampiran 17. Lanjutan
Proses Pengentalan Ekstrak Hasil EEDJB
Proses Pembuatan Basis Gel Karbopol
Alat dan Bahan Pembuatan Gel
101
101
Lampiran 17. Lanjutan
Proses Pembuatan Gel Penimbangan Sediaan Gel
Gel EEDJB 3% Gel EEDJB 5%
Gel EEDJB 4% Basis Gel Karbopol
102
102
Lampiran 17. Lanjutan
Pengujian Sifat Fisika Kimia Gel
Pengujian Viskositas Menggunakan Viskometer Rion
Pengujian pH Menggunakan pH Meter
Pengujian Daya Lekat
104
104
Lampiran 17. Lanjutan
Proses Seleksi Tikus Galur Wistas Proses Aklimitasi Tikus
Sewaktu Pmbelian
Proses Pencukuran Bulu Tikus Anastesi Lokal
Proses Pembuatan Luka Bakar
Alat Solder Pembuat Luka Bakar
105
105
Lampiran 17. Lanjutan
Kandang Tikus Individu
Proses Pengolesan Sediaan Gel EEDJB, Bioplacenton, dan Basis Gel Karbopol
106
106
Lampiran 17. Lanjutan
Pengujian Efektivitas Luka Bakar
Kelompok 1 Gel EEDJB 3%
Sebelum (pada hari ke-0) Sesudah (pada hari ke-17)
Kelompok 2 Gel EEDJB 5%
Sebelum (pada hari ke-0) Sesudah (pada hari ke-17)
Kelompok 3 Gel EEDJB 7%
Sebelum (pada hari ke-0) Sesudah (pada hari ke-17)