Phytochemical profile of Codiaeum variegatum (L.) Bl.

Veni SN., et.al., A, Int. J. Pharmacol. Pharm. Sci. (2015) 2:3; 22-31 22

International Journal of Pharmacology and Pharmaceutical Sciences 2014; Vol: 2, Issue: 3, 22-31.

Review Article

RESEARCH ARTICLE ISSN: 2394-613X

Phytochemical profile of Codiaeum variegatum (L.) Bl. Sangha R Bijekar1*, M.C.Gayatri2 1Department Molecular Biology, Bangalore University, Bangalore-India 2Department of Botany, Bangalore University, Bangalore - India

* Corresponding Author

Abstract

The present study investigates the qualitative and quantitative analysis of the major bioactive constituents of different parts like

root, stem and leaf of medicinally important plant Codiaeum variegatum using seven different solvents. Qualitative study of

alkaloids, carbohydrates, glycosides, steroids, flavonoids, coumarins, saponins, fatty acids, tannins, protein and amino acids, gum

and mucilage, terpenoids, anthroquinones and phenols showed different types of results in different solvents. Quantitative

estimation revealed that phytochemicals are in between the following range alkaloids in between the range of (4.66 -10.2%),

flavonoids (33.1-37.63%), saponins (11.36-13.76%), phenolics (35.43-39.76%), tannins (10.5-18.5%), terpenoids (27.56-

30.3%).

Key Words: Codiaeum variegatum, Euphorbiaceae, phytochemicals screening.

INTRODUCTION

Codiaeum variegatum (L.) Bl. ("garden croton" or "variegated croton"; syn. Croton variegatum L. is a species of plant in the genus

Codiaeum, which is a member of the family Euphorbiaceae. It is native to southern India, Sri Lanka, Indonesia, Malaysia, and the

western Pacific Ocean islands, growing in open forests and scrub (Huxley A, 1992) It is an evergreen shrub growing to 3 m tall and

has large, thick, leathery, shiny evergreen leaves, alternately arranged, 5–30 cm long and 0.5–8 cm broad. The inflorescences are

long racemes 8–30 cm long, with male and female flowers on separate inflorescences; the male flowers are white with five small

petals and 20–30 stamens, the female flowers yellowish, with no petals. The fruit is a capsule 9 mm diameter, containing three 6

mm seeds. The stems contain milky sap that bleeds from cut stems Croton ‘Gold Dust’ is a compact plant with elliptical to oval

leaves which are medium green and liberally dusted with paint-like specks of yellow. Mature crotons are "V" shaped. Crotons grow

best in well-drained soil and need to be shaded from the hottest sun of the day. Too much sun bleaches the color out of the leaves

and too little will make them more green. The leaves extracts of crotons are reported to have many medicinal properties including

purgative, sedative, antifungal, antiamoebic and anticancerous activities (Deshmukh & Borle, 1975; Kupchan et al., 1976). Anti-

inflammatory, Antifungal, antiamoebic and anticancerous activities (Olusola et al., 2007) Used to treat irregular menstruation

(Bourdya et al. 1992), wound healing (Sangeetha et al. 2011). A plant’s medicinal value is due to the presence of some chemical

substance that produces a physiological action on the Human body. These chemicals are of two types, primary and secondary

metabolites. Primary metabolites are those which are required for growth and development of plant and secondary metabolite are

the byproducts of metabolic pathway and play an important role in defense system. Secondary metabolites are such as alkaloids,

carbohydrates, glycosides, steroids, flavonoids, coumarins, saponins, fatty acids, tannins, protein and amino acids, gum and

mucilage, terpenoids, anthroquinones and Phenols. Study of these phytochemicals is crucial because these chemicals can be

exploited to synthesize future drugs.

MATERIAL AND METHODS

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Collection of Plant material

Codiaeum variegatum (L.) Bl. was collected from Lal Bagh Botanical garden, Bangalore and now plant is being maintained in the

Department of Molecular Biology, Bangalore University, Bangalore.

2.2 Preparation of plant extract

Leaf, stem, root and flowers were collected from Codiaeum variegatum. They were dried for one week at room temperature (in

shade). Dried plant parts were grinded in a blender to fine particles. Crude plant extract was prepared by Soxhlet extraction method.

Seven different solvents were used namely methanol, ethanol, water, chloroform, petroleum ether, hexane and acetone. 20gm of

dried fine grinded powder was uniformly packed into thimble and phytochemicals were extracted with 250mL of seven mentioned

solvents separately. The extraction was carried out for 24 hours. Later extract was concentrated by keeping it on hot plate at 30 to

40°C and stored at 4°C for further research.

Phytochemical profiling

Qualitative study

Test for alkaloids (Gibbs 1974)

The test solution of the extracts was dissolved in chloroform and the solution was extracted with Dil. HCL or H2SO4 and acid layer

taken and tested for presence of alkaloids.

Dragendroff’s test

To 2mL of acid layer of test solution, add 2mL of Dragendroff’s reagent (potassium bismuth iodide solution) and 2mL of Dil. HCL.

An orange-red precipitate indicates the presence of alkaloids.

Mayer’s test

To the 1 mL of acid layer of test solution, add 1mL of Mayer’s reagent (potassium mercuric iodide solution). Whitish or cream

colored precipitate indicates the presence of alkaloids.

Wagner’s test

To the 1mL of acid layer of test solution, add 2mL of Wagner’s reagent (iodine in potassium iodide). Reddish brown colored

precipitate indicates the presence of alkaloids.

Anthraquinone (Borntrager’s test)

About 0.5 g of the test solution was taken into a dry test tube and 5 mL of chloroform was added and shaken for 5 min. The extract

was filtered and the filtrate shaken with equal volume of 10% ammonia solution. A pink violet or red color in the ammoniacal layer

(lower layer) indicates the presence of anthroquinone (Aiyelaagbe et al. 2009)

Test for carbohydrates

Molisch’s test

To 2mL of the test solution, add 1mL of α-napthol solution, add concentrated sulphuric acid (H2SO4) through the sides of the test

tube. Purple or reddish violet color at the junction of the two liquids reveals the presence of carbohydrates.

Barford’s test


To 0.5mL of test solution, add 3mL of Barfoed's solution, Place the tubes in a boiling water bath A rusty or brownish-red color will

indicate monosaccharides.

Benedicts test

To 0.5mL of test solution, add 3 ml of Benedict's reagent. Shake each test tube to assure thorough mixing. Place the tubes in a

boiling water bath for 3 minutes. Red or green or yellow ppt obtained shows presence of reducing sugar

Cardiac glycosides (Keller Killiani’s test)

About 100 mg of test solution was dissolved in 1 mL of glacial acetic acid containing one drop of ferric chloride solution. This was

then underlayer with 1 mL of concentrated Sulphuric acid. A brown ring obtained at the interface indicated the presence of Cardiac

glycosides (Aiyelaagbe et al, 2009)

Coumarins test

In a test tube, 1 g of each of the extracts were placed and covered with filter paper moistened with dilute sodium hydroxide (NaOH),

then heated on water bath for a few minutes. The filter paper was examined under UV light, yellow fluorescence indicated the

presence of coumarins (El-Tawil et al., 1983)

Fatty acids test:

5mL of test solution was mixed with 5mL of ether. This extract was allowed to evaporate on filter paper and dried the filter paper.

The transparency on filter paper indicates the presence of fatty acids (Chandrashekar and Rao, 2013)

Test for Flavonoids

Shinoda’s test

About 1 g of each of the extracts was dissolved with 5 ml of ethanol (98 %). To this a small piece of magnesium foil metal was

added, this was followed by drop wise addition of concentrated hydrochloric acid. Intense cherry red colour indicated the presence

of flavonones. Orange red colour indicated the presence of flavonols (Brain et al., 1975).

Lead acetate test

Few drops of lead acetate solution were added to each of the extracts in test tubes. Formation of yellow coloured precipitate indicated

the presence of flavonoids (Tiwari et al., 2011).

Alkaline reagent test

About 1 ml test solution was treated with few drops of sodium hydroxide solution and observed for intense yellow coloration which

disappeared on the addition of dilute HCl. (Veena et al. 2013)

Detection of Gum and Mucilage

The plant extract was dissolved in 10mL of distilled water and to this; 25 mL of absolute alcohol was added with constant stirring.

White or cloudy precipitate indicated the presence of gums and mucilages (Sai Koteswar Sarma et al. 2011).

Ninhydrin test


Ninhydrin test: Add two drops of freshly prepared 0.2% ninhydrin reagent (0.1% solution in n-butanol) to the small quantity of

extract solution and heat. Development of blue color reveals the presence of proteins, peptides, or amino acids. (Saxena Mamta et

al. 2012)

Test for Phenols

Ferric chloride test: To 10mL of alcoholic solution of extract, 2mL of distilled water followed by drops of 10% aqueous Ferric

chloride (FeCl3) solution were added. Formation of blue colour indicates the presence of phenols (Chandrashekar and Rao, 2013)

Test for Saponins

About 0.5 g of the plant extract was shaken with water in a test tube. Frothing, which persist on warming was taking as a preliminary

evidence for the presence of saponin. Few drops of olive oil was added to 0.5 g of the extract and vigorously shaken. Formation of

soluble emulsion in the extract indicates the presence of Saponin (Odebiyi and Sofowora, 1978)

Test for Steroids

Liebermann Burchard Test:To 1mL of extract, 1mL of glacial acetic acid and 1mL of acetic anhydride and two drops of concentrated

sulphuric acid were added. The solution becomes red, then blue and finally bluish green, indicates the presence of steroids (Seema

Firdouse and Parwez Alam 2011)

Tannins test

The extract of the sample was treated with 15% ferric chloride test solution. The resultant colour was noted. A blue colour indicated

the presence of hydrolyzable tannin. Or into 10 mL of freshly prepared potassium hydroxide (KOH) in a beaker, 0.5 g of the extract

was added and shaken to dissolve. A dirty precipitate observed indicates the presence of tannin (Odebiyi and Sofowora, 1978;

Sofowora, 1982).

Test for terpenoids

Salkowski test: To 0.5 mL of each the extract was added 2mL of chloroform. Three mL of concentrated sulphuric acid (H2SO4)

was carefully added to form a layer. A reddish brown colouration of the interface indicates the presence of terpenoids. (Sofowora,

1982)

Phytochemical quantitative test

The extracts were subjected to quantitative phytochemical tests for plant secondary metabolites such as alkaloids, flavonoids,

saponins, phenolics, tannins and terpenoids.

Alkaloid determination using Harborne (1973) method

In 250mL conical flask, 5g of the dried fine powdered sample is taken and 200 ml of 10% acetic acid in ethanol was added and

covered and allowed to stand for 4h. This was filtered and the extract was concentrated on a water bath to one-quarter of the original

volume. Concentrated ammonium hydroxide was added dropwise to the extract until the precipitation was complete. The whole

solution was allowed to settle and the precipitated was collected and washed with dilute ammonium hydroxide and then filtered.

The residue is the alkaloid, which was dried and weighed.

Flavonoid determination by the method of Bohm and Kocipai- Abyazan (1994)

10 g of the plant sample was extracted repeatedly with 100 ml of 80% aqueous methanol at room temperature. The whole solution

was filtered through whatman filter paper No 42 (125 mm). The filtrate was evaporated into dryness over a water bath and weighed.


Saponin determination (Obadoni and Ochuko, 2001)

In conical flask, 20 g of dries fine particles plant sample was takem and 100 mL of 20% aqueous ethanol was added. This mixture

was heated (55˚C) on water bath for 4 h with continuous stirring. Later the mixture was filtered and the residue reextracted with

another 200 ml of 20% ethanol. This extracts was further reduced to 40 mL over hot water bath (90˚C). The concentrated extract

was transferred into a 250 ml separating funnel and 20 ml of diethyl ether was added and shaken vigorously. Ether layer was

discarded and aqueous layer was collected. This step of purification was repeated. 60 ml of n-butanol was added. The combined n-

butanol extracts were washed twice with 10 ml of 5% aqueous sodium chloride. The remaining solution was heated in a waterbath.

After evaporation the samples were dried in the oven and weighed.

Determination of tannin content of the sample (Van Buren, J. P. and Robinson, W. B. 1981):

In 250mL conical flask, 5g of powdered plant sample was taken and 50mL of distilled water was added and shook it vigorously for

an hour. Later this solution was filtered into a volumetric flask and 5mL of this filtrate is pipetted out into a test tube. The sample

were incubated for 1.5 hours at 20 – 300 C and the sample was then filled with distilled water up to mark of 50 mL of the volumetric

flask. Tannic acid was used as standard; 0.1g of tannic acid was dissolved in 100 mL of water to form tannic acid solution. Distilled

water was used as blank. The absorbance of the samples was measured at 760 nm. The values generated were used to calculate the

tannin content.

Determination of total phenols (Edeoga et al., 1983 and Jing-Chung et al., 2007):

The sample was boiled with 50 ml of ether for the extraction of the phenolic component for 15 min. 5 ml of the extract was taken

into a 50 ml flask, then 10 ml of distilled water was added. 2 ml of ammonium hydroxide solution and 5 ml of concentrated amyl

alcohol were also added and left to react for 30 min for colour development. This was measured at 505 nm. The standard curve was

prepared using 0, 50, 100, 150, 200, 250 mg/l solutions of gallic acid in methanol: water (50:50 v/v)

RESULTS AND DISCUSSION

Codiaeum variegatum is an ornamental plant and also known for its medicinal properties. The medicinal properties of plants are

due to the phytochemicals. These phytochemicals are the secondary metabolites which are produced in high quantity under stressed

conditions, which allows the plant to protect itself from adverse environmental factors. Dietary intake of these phytochemicals may

promote health benefits, protecting against chronic degenerative disorders, such as cancer, cardiovascular and neurodegenerative

diseases. Majority of foods, such as whole grains, beans, fruits, vegetables and herbs contain phytochemicals. These phytochemicals,

either alone or in combination, have tremendous therapeutic potential in curing various ailments (Bhatt et al., 1982). The results of

qualitative test of phytochemicals are presented in table 1. Different phytochemicals viz. alkaloids, anthroquinone, carbohydrates,

cardiac glycosides, coumarins, fatty acids, flavonoid, gum and mucilage, protein and amino Acids, phenols, saponin, steroids,

tannins and terpenoids were found to be present in different solvents depending upon their solubility. This result agrees with the

findings of Sangha R.B. et al., (2014) on qualitative phytochemical work carried on Baliospermum montanum (member of

Euphorbiaeae family).

Table 1- Qualitative analysis of different parts like root, stem, leaf, flower and latex of Codiaeum variegatum using

different Solvents-

Plant name W Ac Et Mt Ch Hx Eth Tests Phytochemicals

Cv(leaf) - - + - - - +

Dragendorff’s

Cv (stem) - - + - - - +

Cv (roots) - - + - - - +


Cv(leaf) - - + - - - +

Mayer’s test

Alkaloids

Cv (stem) - - + - - - +

Cv (roots) - - + - - - +

Cv(leaf) - - + - - - +

Wagner’s test Cv (stem) - - + - - - +

Cv (roots) - - + - - - +

CV (leaf) - - + - + + +

Borntreger’s test

Anthroquinones CV (stem) - - + - + + +

CV (roots) - - + - + + +

Cv(leaf) + - + + - - +

Molisch’s test

Carbohydrates

Cv (stem) + + + + - - -

Cv (roots) - + + + - - -

Cv(leaf) + - + + - - +

Barford’s test Cv (stem) + + + + - - -

Cv (roots) - + + + - - -

Cv(leaf) + - + + - - +

Benedicts test Cv (stem) + + + + - - -

Cv (roots) - + + + - - -

Cv(leaf) + + + + + - - Keller- Killiani

test

Cardiac Glycosides Cv (stem) + + - + - - -

Cv (roots) + + - + - -

Cv(leaf) - - - - - + -

Using NaOH

Coumarins Cv (stem) - - - - - - +

Cv (roots) - - - - - - -

Cv(leaf) - - - - - - -

Paper test

Fatty acids test Cv (stem) - - - - - - -

Cv (roots) - - - - - - -

Cv(leaf) - + + + + - -

Shinoda test

Flavonoids

Cv (stem) - + + + + - -

Cv (roots) - + + + + -

Cv(leaf) + + + + + + -

Alkaline reagent

test Cv (stem) - + + + + - +

Cv (roots) - + + + + -

Cv(leaf) + + + + + - -

Lead acetate test Cv (stem) - - + + + - +

Cv (roots) - - + + + - -


Cv(leaf) - - - - - - -

Gum and Mucilage Cv (stem) - - - - - - -

Cv (roots) - - - - - - -

Cv(leaf) + + - - - - -

Ninhydrin test

Protein and Amino

Acids Cv (stem) + - - + - - -

Cv (roots) + - - + - - -

Cv(leaf) - + + + - - -

Ferric chloride

test

Phenols Cv (stem) - + + + - - -

Cv (roots) - + + + - - -

Cv(leaf) + - + + - - +

Frothing test

Saponins Cv (stem) + - + + - - -

Cv (roots) + - + + - - -

Cv(leaf) - - - - - - +

Liebermann

Burchard reaction

Steroids Cv (stem) - - - - + + +

Cv (roots) - - + - - -

Cv(leaf) + - + + - - -

Using FeCl3

Tannins test Cv (stem) + - + + - - -

Cv (roots) + - + + - - -

Cv(leaf) - + - + + - -

Salkowski test

Terpenoids Cv (stem) - - - - - - -

Cv (roots) - - - - - - -

CV= Codiaeum variegatum, W=water, Ac=Acetone, Et= Ethanol, Mt= Methanol, Ch= Chloroform, Hx=Hexane, Eth= ether, + =

present, - = absent

Fig:1- Quantitative estimation (%) Secondary metabolites of different parts of Codiaeum variegatum, n=3, Data is

presented as Mean± SD.

05

1015202530354045

Qu

anti

tati

ve e

stim

atio

n

Phytochemicals

leaf

stem

root


Quantitative estimation revealed that alkaloid is in between the range of (4.66 -10.2%), flavonoids (33.1-37.63%), saponins (11.36-

13.76%), phenolics (35.43-39.76%), tannins (10.5-15.32%), terpenoids (27.56-30.3%). It has also found that the plant is rich in

flavonoids, phenols and terpenoids. The estimated quantitative data is presented in Fig 1.

The Codiaeum variegatum is known to have medicinal properties like anticancerous and anti-inflammatory properties. This study

revealed the presence of medicinal active constituents of Codiaeum variegatum. The phytochemicals like flavonoids, phenols and

terpenoids which are rich in Codiaeum variegatum may be contributing in providing the medicinal properties to the plant. The

inflammation is mainly caused by cyclooxygenase and lipooxygenase pathway. Phenolic compounds were shown to inhibit the

cyclooxygenase and lipooxygenase pathway (Ferrandiz et al. 1991; Ferrandiz et al. 1991; Laughton et al. 1991). Flavonoids are

found to inhibit Ornithine decarboxylase enzyme, it is rate-limiting enzyme in polyamine biosynthesis, which has been correlated

with the rate of DNA synthesis and cell proliferation in several tissues, hence inhibiting cell proliferation (Tanaka et al., 1997a;

Tanaka et al., 1997b; Makita et al., 1996). Flavonoids inhibit growth of microorganism by depolarizing membrane, inhibiting DNA,

RNA and protein synthesis (Dzoyem et al., 2013). Study of flavonoids, phenols and terpenoids from this plant may help us to exploit

more of the medicinal properties of Codiaeum variegatum.

CONCLUSION

The Codiaeum variegatum was screened for phytochemical constituents and found to be good source of medicinally active elements

which can be further exploit to isolate and synthesize modern medicines. This work justifies the need to isolate and characterize the

medicinally active compounds.

ACKNOWLEDGEMENT

UGC BSR Faculty fellow grant by UGC, New Delhi sanctioned to Dr. M.C.Gayatri is gratefully acknowledged.

Conflict of interest: ‘No conflict to be disclose’’.

REFERENCES

Aiyelaagbe OO and Paul Osamudiamen M. Phytochemical Screening for Active Compounds in Mangifera indica Leaves from

Ibadan, Oyo State 2009; vol.2(1) 11-13.

Boham BA and Kocipai-Abyazan R. Flavonoids and condensed tannins from leaves of Hawaiian Vaccinium vaticulatum and V.

Calycinium. Pacific Science 1974; 48: 458-463.

BOURDYA G & WALTERB. A Maternity and medicinal plants in Vanuatu The cycle of reproduction Journal of

Ethnopharmacology. 1992 37:179-196.

Bhatt AV, Nair KV, Nair CAA and Puri HS. Ethno-botanical studies in the Silent valley and adjoining areas. Bulletin of Medico-

Ethnobotanical Research 1982 vol.3 153-161.

Brain KR and Turner TD. The practical evaluation of phytochemicals. Wright Science Technical: Bristol 1975.

Chandrashekar R and Rao SN. Phytochemical analysis of ethanolic extract of leaves of leucas indica (eelli) Int J Pharm Bio Sci

2013; 4(1): 33 – 38.

Deshmukh SD and Borle MN. 1975. Studies on the insecticidal properties of indigenous plant products. Ind J Ent 1975;

37(1):11-18.

Dzoyem JP, Hamamoto H, Ngameni B, Ngadjui BT and Sekimizu K. Antimicrobial action mechanism of flavonoids from

Dorstenia species. Drug Discov Ther 2013;7(2):66-72.

http://scialert.net/asci/author.php?author=O.O.&last=Aiyelaagbe

http://scialert.net/asci/author.php?author=Paul%20M.&last=Osamudiamen


Edeoga1 HO, Okwu DE and Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants” African Journal of

Biotechnology 2005; 4 (7): 685-688.

El-Tawil BDH. Chemical constituents of indigenous plants used in Native Medicines of Saudi Arabia, II. Arab Gulf J Sci ResA

1983; 1(12):395 -410.

Ferrandiz ML, Nair AG and Alcaraz MJ. Inhibition of sheep platelet arachidonate metaboilm by flavonoidsfrom Spanish and Indian

medicinal herbs. Pharmazie 1990; 45: 206-208.

Ferrandiz ML and Alcaraz MJ. Antiinflammatory activity and anhibition of archidonic acid metabolism by flavonoids. Agents

Actions. 1991; 32:283-288.

Gibbs RD. Chemotaxonomy of Flowering Plants. McGill-Queen's University Press, Montreal and London 1974; vol.1.

Harborne JB. Phytochemical methods, London. Chapman and Hall Ltd. 1973; 49-188.

Huxley A. ed. New RHS Dictionary of Gardening. Macmillan 1992; 1: 665.

Jing-Chung Chen, Jan-Ying Yeh, Pei-Chun Chen and Cheng-Kuang Hsu. Phenolic content and DPPH radical scavenging activity

of Yam-containing Surimi gels influenced by salt and heating. Asian Journal of Health and Information Sciences 2007; 2(1-4): 1-

11.

Kupchan SM, Uchida I, Branfman AR, Dailey RC and Fei BY. Antileukemic principles isolated from Euphorbiaceae. Plants.

Sci. 1976; 191:571-572.

Laughton MJ, Evans PJ, Moroney MA, Hoult JR and Halliwell B. Inhibition of mammalian 5-lipoxygenase and cyclooxygenase

by flavonoids and phenolic dietary additives. Relationship to antioxidant activity and to iron-reducing ability. Biochem Pharmacol

1991;42: 1673-1681

Makita H, Tanaka T, Fujitsuka H, Tatematsu N, Satoh K, Hara A, Mori H. Chemoprevention of 4-nitroquinoline 1-oxide-induced

rat oral carcinogenesis by the dietary flavonoids chalcone, 2-hydroxychalcone, and quercetin. Cancer Res 1996; 56:4904–4909.

Obdoni BO and Ochuko PO. Phytochemical studies and comparative efficacy of the crude extracts of some Homostatic plants in

Edo and Delta States of Nigeria. Global J. Pure Appl. Sci. 2001; 8: 203-208.

Odebiyi A and Sofowora AE. Phytochemical screening of Nigeria medicinal plant. part III Lioydia 1978; 4: 234-246.

OLUSOLA K, OGUNWENMO OLUWATONI A and IDOWU CHUKWUDI INNO. Cultivars of Codiaeum variegatum (L.)

Blume (Euphorbiaceae) show variability in phytochemical and cytological characteristics African Journal of Biotechnology 2007;

Vol. 6 (20) 2400-2405.

Sai Koteswar Sarma D, Venkata Suresh Babu A. Pharmacognostic and phytochemical studies of Thespesia populnea Linn J. Chem.

Pharm. Res. 2011; 3(4):237-244.

Sangeetha G , Mohan Krishna L, Aruna G, Sekar Babu M, Balammal G. Study on wound healing activity of root of Codiaeum

variegatum. Innovative Drug Discovery 2011;1(1):19-23.

Sangha R Bijekar, Gayatri MC, and Rajanna L. Phytochemical profile of Baliospermum montanum (Wild.) Muell. Arg.

International Journal of Innovation and Scientific Research 2014; 12 (1) 37-42.

Saxena Mamta and Saxena Jyoti. Phytochemical screening of Acorus Calamus and Lantana Camara International research Journal

of Pharmacy 2012; 3 (5) 323-326.


Seema Firdouse, Parwez Alam. Phytochemical investigation of extract of Amorphophallus campanulatus tubers International

Journal of Phytomedicine 2011; 3: 32-35.

Sofowora A. Medicinal plants and traditional medicine in Africa. John Wiley, Chichester (1982; pp. 179.

Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: A review. Internationale Pharmaceutica

Sciencia 2011; 1(1): 98-106.

Tanaka T, Makita H, Kawabata K, Mori H, Kakumoto M, Satoh K, Hara A, Sumida T, Tanaka T and Ogawa H. Chemoprevention

of azoxymethane-induced rat colon carcinogenesis by the naturally occurring flavonoids, diosmin and hesperidin. Carcinogenesis

1997a; 18:957–965.

Tanaka T, Makita H, Ohnishi M, Mori H, Satoh K, Hara A, Sumida T, Fukutani K, Tanaka T and Ogawa H. Chemoprevention of

4-nitroquinoline 1-oxide-induced oral carcinogenesis in rats by flavonoids diosmin and hesperidin, each alone and in combination.

Cancer Res 1997b; 57:246–252.

Veena Sharma, Aastha Agarwal, Urmila Chaudhary, Manu Singh phytochemical investigation of various extracts of leaves and

stems of Achyranthes aspera Linn. International Journal of Pharmacy and Pharmaceutical Sciences 2013; Vol 5, Suppl 1.

Van Buren JP and Robinson WB. Formation of Complexes between Protein and Tannic Acid, Journal of Agric Food Chemistry

1981; 17: pp 772 – 777.

Phytochemical profile of Codiaeum variegatum (L.) Bl.

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