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Proco eedingsSeminar Reakror Nuklir dalam Penelitian Sains

da..1Tekrwlogi Menuju Era Tinggal Landas

Bandung

8-

10 Okrober 1991

PPTN

BATAN

IODINASI 1-125 FSH MENGGUNAKAN OKSIDATORN-BROMO

SUKSINIMID, LAKTOPEROKSIDASE DAN IODOGEN

Sukiyati Dj.,Wayan R.S, Gina M,A.Ariyanto.

Pusat Produksi Radioisotop - Badan Tenaga Atom Nasional

AUSTRAK

IODINASI (1-125) FSH MENGGUNAKAN OKSIDATOR N-BROMO SUKSINIMID,

LAKTOPEROKSIDASE DAN IODOGEN. FSH 1251adalah salah satu pereaksi Kit RIAyang

digunakan untuk menentukan konsentrasi FSH di dalam serum darah serta mengetahui

penyimpan~an koordinasi kelenjar hipotalamus, pituitari dan gonad. Untuk dapat mempro

duksi FSH1 51yang bermutu baik sebagai komponen pereaksi Kit RIAFSH, dalam penelitian

ini telah dilakukan iodinasi FSH dengan 1-125,menggunakan oksidator N-bromo suksinimid,

laktoperoksidase, dan iodo bead sebagai aseptor elektron :

1251

Terhadap masing-masing hasil penandaan dilakukan penentuan penandaan dan uji imuno

logi serta ikatan non spesifik (NSB) dengan membandingkannya dengan persen

)

ikatan

(Btr) Kit RIAFSH dari Amersham. Stabilitas ikatannya dalam penyimpanan pada 4 c juga

ditentukan. Dengan oksidator N-bromo suksinimid, lakto peroksidase dan iodogen diperoleh

hasil berturut-turut : persen penandaan 25 ,8,2 dan 18 ,nilai Btrsebesar 22 ,34

dan 25 serta NSB masing-masing sebesar 1,3; 2,3 dan 0,6 .Uji stabilitas menunjukkan

bahwa FSH_1251masih baik pada penyimpanan selama satu bulan pada suhu 4C.

AJBSTRACT

THE USE OF N-BROMOSUCCINIMIDE, LACTOPEROXIDASEAND IODOGEN FOR

IODINATIONOF FOLLICLE STIMULATINGHORMONE (FSH).FSH_1251s one ofthe FSH

RIA kit reagent utilize for determination of FSH concentration in blood serum, to study the

coordinationdisorder of the principle hormones, i.e., hypothalamus, pituitary and gonads. To

produce a goodquality ofFSH

125,1

as a component ofFSH RIAkit, a research on the iodination

(1-125)of FSH employing mild oxidant N-bromosuccinimid, lactoperoxidase, and iodo-bead

has been performed.

1251 I 1251+ + e- FSH +1251+ I FSH1251 + H+

The percentage oflabeling, the immunology activity and Non Spesific Binding ofthe labeled

products were determined. The immunology activity in ) T and non spesific binding )

NSB were compare with Amersham FSH RIAkit. The stability ofthe binding is also be checked

by storing the labeled products at 4C. With N-bromosuccinimid, lactoperoxidase and iodogen

as the oxidizing agents, the results obtained were respectively as follows : percentages of

labelling were 25 ,8.2 and 18 . T values were 22 ,34 and 25 ,and the NSB's

were 1.3, 2.6 and 0.6 .The stability test of labeled FSH.1251showed that it does not

deteriorate significantly after storage for one month at 4C.

DAFfAR PUSTAKA

1. Thorell, J. I. and Larson, I. , Lactoperoxidase coupled to polyacrylamide for radio-iodination

ofprotein's to high specificactivity, Immunochemistry II, USA (1974)203.

2. Paul Reay, Use of N-Bromo succinimide for the iodination of protein for radioimmunoassay,

Ann. Clin. Biochem 19 (1982) 129 - 133.

3. IAEA, Laboratory Training Manual on Radioimmunoassay in Animal Reproduction, Thctlni

cal Report Series. No.233,Vienna (1984) 186 - 193.

4. IAEA, Proceedings ofa Medical Applications ofNuclear Techniques in Developing Countries,

IAEA in co-operation with the World Health Organization and Held inVienna (26 - 30 August

1985).

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5. IAEA, Regional Training Course on Production and Use ofBulk Reagents for RIA ofThyroid

Related Hormones, Bangkok, Thailand 8 . 19December 1986 .

6. IAEA, Regional Course on Optimization of Production Techniques and Distribution Schemes

for Reagent for Radioimmunoassay, held at National Institute of Health Nonthaburi,

Thailand 9 20th October 1989 .