Sequenching by Hybridization

(SBH)

The next generation of sequenching technologies

Ziebolz B, 2006, Next generation sequenching technologies, BTi.

Sequenching by HybridizationPrinsip:

Perbedaan hibridisasi dari pelacak oligonukleotidayang dapat digunakan untuk membaca kode genetik dari DNA target.

Pendekatan:1. Imobilisasi DNA pada membran yang akan menginisiasi hibridisasi yang

dideteksi oleh probe oligonukleotida pendek.2. Hibridisasi DNA ke microfabricated tilling arrays immobilized

oligonucleotide, with > 100.000 copies of each induvidual of feature.

Mekanisme SBH

To resequence a given base, four features are present on the microarray, each identical except for a different nucleotide at the query position (the central base of 25-bp oligonucleotides). Genotyping data at each base are obtained through the differential hybridization of genomic DNA to each set of four features.

Jay Shendure*, Robi D.Mitra‡, Chris Varma* and George M. Church*, 2005, ADVANCED SEQUENCING TECHNOLOGIES: METHODS AND GOALS, Nature Review, Volume 5.

Lanjutan…

MIcroarrayDNA microarray analysis is one of the fastest-growing new technologies in the field of genetic research. The basis of DNA microarray is sequencing by hybridization.

Aplikasi SBHIdentification of FVIII gene mutations in patients with hemophilia A