SABUN CUCI PIRING ( ) SEBAGAI PENGGANTI XYLOL PADA …repository.unimus.ac.id/1437/1/ABSTRAK.pdf ·...
Transcript of SABUN CUCI PIRING ( ) SEBAGAI PENGGANTI XYLOL PADA …repository.unimus.ac.id/1437/1/ABSTRAK.pdf ·...
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SABUN CUCI PIRING (DISHWASHING SOAP) SEBAGAI
PENGGANTI XYLOL PADA PROSES DEPARAFINASI
PENGECATAN IMUNOHISTOKIMIA HER2
Ardi Pranata 1, Sri Sinto Dewi
2, Arya Iswara
2
1. Program Studi D IV Analis Kesehatan Fakultas Ilmu Keperawatan dan Kesehatan
Universitas Muhammadiyah Semarang
2. Laboratorium Biologi Molekuler Fakultas Ilmu Keperawatan dan Kesehatan
Universitas Muhammadiyah Semarang
ABSTRAK
Kanker payudara memiliki tiga biomarker molekular yaitu estrogen reseptor (ER),
progesteron reseptor (PR), dan HER2 yang diadopsi sebagai pemeriksaan rutin
yang berperan menentukan prognosis dan prediktif kanker. Umumnya
pemeriksaan biomarker tersebut dilakukan dengan metode imunohistokimia.
Penggunaan xylol pada proses deparafinasi pengecatan HER2 diganti dengan
sabun cuci piring. Tujuan penelitian mengetahui gambaran hasil pengecatan
HER2 menggunakan 1,5%, 2%, 2,5% sabun cuci piring dan analisis perbedaan
hasil pengecatan dibandingkan dengan xylol. Penelitian secara eksperimental
dengan pendekatan cross sectional. Sampel penelitian menggunakan jaringan
kanker payudara HER2 positif skor +3 dari satu pasien. Metode pengecatan HER2
menggunakan teknik Strep(Avidin) Biotin Complex. Pengecatan HER2
menggunakan sabun cuci piring 1,5%, 2%, 2,5% dan xylol sebagai kontrol. Hasil
pengecatan HER2 menggunakan xylol skor +3, sabun cuci piring 1,5% skor +2,
sabun cuci piring 2% dan 2,5% skor +3. Terdapat perbedaan yang bermakna
antara xylol dengan sabun cuci piring 1,5%. Tidak terdapat perbedaan yang
bermakna antara xylol dengan sabun cuci piring 2% dan 2,5%. Xylol pada proses
deparafinasi pengecatan imunohistokimia HER2 dapat diganti dengan sabun cuci
piring 2%.
Kata kunci: HER2, xylol, deparafinasi, sabun cuci piring
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DISHWASHING SOAP AS A SUBSTITUTE
FOR XYLOL IN DEPARAFFINIZATION
IMMUNOHISTOCHEMISTRY STAINING OF HER2
Ardi Pranata 1, Sri Sinto Dewi
2, Arya Iswara
2
1. Department of D IV Medical Laboratory Technology of Nursing and Health Science
Faculty of Muhammadiyah University of Semarang
2. Molecular Biology Laboratory of Nursing and Health Science Faculty of
Muhammadiyah University of Semarang
ABSTRACT
Breast cancer has three molecular biomarker that is estrogen receptor (ER),
progesterone receptor (PR), and HER2 adopted as a routine examination that
played in determining the prognosis and predictive cancer. Generally, the
biomarker examination conducted by immunohistochemical methods. The use of
xylol in HER2-staining deparaffinization process replaced with dishwashing soap.
Purpose of the research was to described the result of staining using 1.5%, 2%,
2.5% of dishwashing soap and analysis difference of staining result compared
with xylol. This research was experimental with cross-sectional approach. Sample
of the research using HER2-positive breast cancer with score +3 from single
patient. Methode of HER2-staining used Strep(Avidin) Biotin Complex technique.
HER2-staining used 1,5%, 2%, 2,5% of dishwashing soap and xylol as control.
The result of HER2-staining used xylol as deparaffinization agent showed
obtained score +3, used 1,5% dishwashing soap obtained score +2, while 2% and
2.5% dishwashing soap obtained score +3. There is a significant difference
between xylol with dishwashing soap 1.5%. There was no significant difference
between xylol with dishwashing soap 2% and 2.5%. Xylol on HER2
immunohistochemical staining deparaffinization process can be replaced with
dishwashing soap 2%.
Keyword: HER2, xylol, deparaffinization, dishwashing soap
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