LAMPIRAN semangat

LAMPIRAN

Lampiran 1 Data jumlah AgNOR pada seluruh kelompok

Kontrol 1

3 5 7 5 4 7 6 7 6 6 Jumlah8 5 4 5 5 5 9 5 6 5

611

6 5 4 5 6 4 6 6 5 77 5 6 5 6 8 6 6 5 79 8 10 8 5 9 9 8 7 96 4 5 4 8 4 9 4 5 76 4 7 8 6 5 6 7 7 54 4 6 4 6 7 6 4 8 56 4 5 5 9 7 5 6 6 46 7 11 7 8 11 5 4 7 7

Rata rata 6,11

Kontrol 2

5 5 7 5 7 5 7 6 10 14 Jumlah5 7 8 5 5 5 6 9 9 10

740

11 11 5 10 10 11 7 8 5 37 6 8 8 14 11 8 6 5 66 7 6 5 6 6 7 6 11 87 5 8 7 6 8 10 4 6 66 5 9 5 6 6 5 6 9 96 11 6 5 6 6 6 4 9 9

11 7 8 12 9 9 9 8 11 94 7 8 7 6 5 11 10 8 12

Rata rata 7,4

Kontrol 3

6 10 4 6 8 7 5 6 6 6 Jumlah10 6 4 7 6 6 4 10 10 4

631

10 10 9 6 4 6 4 6 8 86 4 6 7 10 6 4 8 4 44 4 4 8 4 6 4 10 6 6

10 4 4 8 4 6 6 4 8 44 10 6 5 4 6 4 10 6 67 6 6 8 6 6 4 4 10 4

10 6 10 6 6 8 4 5 4 108 6 8 6 8 8 4 6 7 4

Rata rata 6,31

Kontrol 4 4 4 8 4 4 4 4 4 8 9 Jumlah8 4 7 4 4 4 4 4 6 4

518

8 4 8 6 6 6 5 4 8 47 5 7 10 6 4 4 4 4 66 4 8 10 6 6 4 4 5 44 8 6 4 4 5 4 4 6 4

8 4 4 6 6 4 10 4 10 44 4 4 4 4 4 4 4 4 44 4 4 4 5 4 4 4 4 5

8 4 4 4 6 4 10 4 4 4Rata rata 5,18

Kontrol 5

4 5 4 4 3 6 6 7 7 5 Jumlah5 5 7 5 4 4 3 4 6 7

568

3 5 5 3 8 6 3 4 8 66 5 4 4 6 5 7 6 4 64 5 4 5 3 6 7 5 6 83 5 4 4 4 6 4 6 7 94 4 7 4 6 4 5 6 7 74 4 5 6 5 4 8 6 5 77 6 8 7 7 9 7 8 6 79 7 6 8 10 7 6 8 7 10

Rata rata 5,68

Kontrol 6

4 4 10 10 8 8 6 4 8 4 Jumlah4 6 6 6 4 4 10 8 6 10

658

4 6 4 6 6 6 8 6 4 66 6 10 10 4 4 8 6 6 10

10 10 8 10 4 8 6 4 8 86 10 10 6 8 6 4 4 8 66 6 10 6 6 6 4 8 4 66 10 6 10 6 8 6 4 6 48 10 6 8 6 6 8 6 4 44 6 10 10 6 4 8 4 8 4

Rata rata 6,58

Dosis 1,1

5 7 4 2 4 4 5 2 2 5 Jumlah2 4 3 2 4 5 4 2 2 4

329

3 6 5 3 6 2 2 3 2 43 3 3 3 3 5 4 2 1 32 4 4 3 2 2 2 4 1 45 9 3 4 1 5 2 3 4 44 4 3 4 4 6 2 4 4 32 5 5 3 3 2 2 1 2 3

2 4 3 3 2 2 5 3 3 42 3 2 2 3 3 3 3 3 2

Rata rata 3,29

Dosis 1,2

7 4 6 5 5 5 4 4 6 4 Jumlah4 3 5 4 9 5 4 3 6 4

488

4 5 3 8 5 4 4 3 4 63 6 5 7 7 4 9 4 3 65 4 7 5 5 5 4 3 3 64 6 5 5 6 4 6 5 9 44 4 4 3 8 5 4 4 7 56 4 8 5 4 4 6 4 8 86 3 4 4 8 4 5 6 8 54 3 3 3 4 3 3 3 3 3

Rata rata 4,88

Dosis 1,3

3 2 5 2 3 4 4 3 4 5 Jumlah3 5 4 2 3 3 4 3 3 3

346

2 2 4 5 3 4 4 4 3 34 3 4 2 4 4 9 4 3 23 5 3 3 6 2 3 4 4 46 2 5 2 2 3 5 6 4 62 2 2 2 2 3 3 3 2 23 2 4 5 3 3 2 4 4 23 3 5 2 4 3 2 4 4 24 3 3 3 6 3 5 3 4 6

Rata rata 3,46Dosis 1,4 3 8 4 4 6 5 4 5 5 9 Jumlah

4 10 4 3 4 7 6 4 5 4

490

4 7 5 3 6 5 4 4 3 63 6 4 5 7 4 7 8 5 54 4 3 3 4 3 3 7 7 64 8 5 7 5 4 6 4 11 73 3 5 8 5 4 3 6 6 83 6 5 8 4 7 3 5 7 83 3 3 7 4 3 5 8 4 52 2 4 3 3 3 3 2 3 3

Rata rata 4,9

Dosis 1,5

4 3 5 6 7 4 7 5 5 6 Jumlah4 4 6 8 7 4 6 3 9 5

498

3 3 7 6 5 6 6 5 8 64 3 3 5 3 5 4 4 4 54 6 4 3 4 3 7 4 7 75 3 4 5 6 5 3 4 4 9

8 3 3 5 8 5 7 4 4 53 5 9 3 5 6 3 4 9 135 8 5 4 8 5 4 3 6 54 4 3 3 4 3 3 3 4 2

Rata rata 4,98

Dosis 1,6

4 3 6 4 4 3 7 3 3 4 Jumlah5 5 5 3 3 3 5 3 3 7

404

3 5 4 4 4 4 3 5 3 25 4 3 6 4 4 4 3 3 64 4 4 3 5 6 6 4 2 32 2 3 3 8 6 5 4 6 54 5 5 5 4 4 4 3 7 52 4 6 4 7 6 3 4 2 62 3 3 5 6 6 4 2 3 73 3 2 3 2 3 3 2 2 3

Rata rata 4,04

Dosis 2,1

5 5 4 5 4 7 2 3 4 2 Jumlah8 6 6 4 3 4 5 4 6 7

398

5 3 6 3 4 7 6 3 4 66 2 3 5 2 3 3 4 4 64 5 3 2 2 1 4 4 3 75 4 5 3 4 6 2 3 2 63 3 4 2 4 3 5 5 6 75 3 3 3 4 6 3 2 4 53 5 5 2 6 5 2 4 2 22 3 2 4 3 3 2 4 2 3

Rata rata 3,98

Dosis 2,2

3 5 7 4 3 2 7 3 5 4 Jumlah7 5 4 3 6 3 6 1 3 4

432

6 7 7 1 5 4 4 3 5 87 9 7 5 3 4 7 5 2 45 4 3 5 3 7 1 1 4 37 5 7 4 5 9 8 3 5 28 3 6 4 3 4 7 6 3 26 5 6 3 3 3 7 2 5 14 8 5 3 5 2 4 1 3 42 3 4 2 2 2 2 3 4 3

Rata rata 4,32

Dosis 2,35 8 4 3 3 3 3 12 6 2 Jumlah3 4 9 3 2 2 6 7 6 8

4465 4 6 3 3 5 5 8 10 5

6 4 4 6 9 4 4 3 3 35 6 5 3 3 3 5 4 8 13 6 7 2 2 6 3 3 5 24 4 7 4 4 7 1 4 4 16 10 5 3 3 3 5 6 7 33 9 7 5 3 3 4 6 4 23 3 2 4 3 1 3 4 2 3

Rata rata 4,46

Dosis 2,4

2 1 1 1 4 1 1 1 1 1 Jumlah1 1 1 1 4 1 2 2 1 1

145

1 1 2 2 1 1 1 1 3 11 2 1 1 3 2 1 1 1 11 1 1 1 1 1 1 2 1 51 1 2 3 1 1 1 1 2 31 1 1 1 2 1 1 2 1 11 1 1 1 1 4 1 2 1 11 1 1 1 1 1 1 1 2 42 2 2 1 2 2 2 1 2 1

Rata rata 1,45

Dosis 2,5

3 2 2 1 4 2 3 4 2 3 Jumlah1 3 2 1 2 3 2 2 5 1

273

2 4 3 2 2 2 2 3 5 34 4 3 2 3 2 3 4 5 13 4 3 3 4 1 3 2 4 23 3 4 3 4 2 3 3 2 35 1 2 2 2 1 1 4 1 41 6 4 3 4 2 3 4 3 12 5 4 3 2 5 4 2 4 32 2 3 2 1 2 1 1 3 2

Rata rata 2,73

Dosis 2,6

1 1 2 3 1 1 1 3 1 1 Jumlah1 1 1 2 1 1 3 2 2 1

160

1 1 1 3 1 2 2 1 3 21 1 2 1 1 1 1 3 2 31 3 1 2 2 2 2 2 1 31 1 1 1 1 2 1 2 1 12 1 1 1 3 2 3 3 1 32 3 2 1 4 1 1 1 1 31 4 1 1 2 1 1 1 1 11 1 1 2 2 1 1 1 1 2

Rata rata 1,6Dosis 3,1 6 2 4 4 3 1 3 1 6 4 Jumlah

5 5 5 3 3 2 4 4 4 6

308

3 4 4 2 2 1 3 1 1 46 3 5 4 3 2 3 1 4 23 3 6 6 5 2 2 5 2 32 3 2 2 2 2 5 2 1 53 3 2 1 2 2 3 2 3 53 5 4 4 6 2 3 4 5 33 4 2 1 4 2 3 2 4 43 1 3 2 2 1 2 1 1 2

Rata rata 3,08

Dosis 3,2

1 1 2 4 2 5 1 1 1 1 Jumlah4 4 1 5 3 4 4 1 1 2

229

1 4 1 3 2 3 1 3 4 21 3 2 1 1 1 1 3 4 11 2 6 1 2 2 2 2 2 22 2 4 1 3 2 3 1 2 21 2 4 3 3 3 2 1 1 43 2 6 3 2 3 5 1 1 11 1 1 5 2 1 6 3 4 21 3 2 2 3 1 1 2 2 1

Rata rata 2,29

Dosis 3,3

7 3 8 3 3 12 2 2 4 3 Jumlah5 2 8 8 7 6 5 6 2 3 4154 2 7 7 5 4 5 3 2 33 6 6 7 4 6 4 3 2 44 3 8 6 7 6 4 3 3 43 2 5 7 4 4 7 8 1 24 1 5 5 3 6 2 1 4 26 4 2 5 5 7 3 4 2 44 5 9 3 2 6 3 3 4 32 3 1 3 2 2 1 2 2 3

Rata rata 4,15

Dosis 3,4

2 5 2 2 4 3 3 3 4 5 Jumlah2 3 4 4 3 5 1 6 5 6

377

2 2 6 4 2 4 6 5 5 66 6 6 3 2 3 7 4 3 64 9 7 4 3 6 7 3 3 72 5 2 3 4 4 4 2 4 42 4 3 2 4 6 6 2 3 74 1 4 2 2 1 3 4 3 65 6 1 2 3 3 4 7 5 52 2 2 1 3 2 1 2 3 2

Rata rata 3,77

Dosis 3,5

3 3 4 2 1 3 4 3 3 5 Jumlah3 3 1 2 2 1 3 2 2 3

279

4 3 3 3 2 2 3 2 2 24 3 3 4 2 5 6 2 2 35 2 3 2 2 1 3 3 1 32 3 2 2 3 3 1 3 4 33 3 3 2 4 5 4 2 3 49 1 2 5 3 3 1 2 2 25 4 6 3 3 3 2 4 3 42 2 2 1 2 1 1 1 2 1

Rata rata 2,79

Dosis 3,6

2 5 2 2 4 3 3 3 4 5 Jumlah2 3 4 4 3 5 1 6 5 6

377

2 2 6 4 2 4 6 5 5 66 7 7 3 2 3 6 4 3 64 7 7 4 3 6 7 3 3 72 5 2 3 4 4 4 2 4 42 4 3 2 4 6 6 2 3 74 1 4 2 2 1 3 4 3 65 7 1 2 3 3 4 7 5 52 2 2 1 3 2 1 2 3 2

Rata rata 3,77

Lampiran 2 Descriptive Test Sebelum transformasi

Descriptives

Dosis Statistic Std. Error

agnor kontrol Mean 6,2100 ,31170

95% Confidence Interval for

Mean

Lower Bound 5,4087

Upper Bound 7,0113

5% Trimmed Mean 6,2011

Median 6,2100

Variance ,583

Std. Deviation ,76352

Minimum 5,18

Maximum 7,40

Range 2,22

Interquartile Range 1,23

Skewness ,333 ,845

Kurtosis ,400 1,741

dosis 1

Mean 4,1567 ,28688


Mean

Lower Bound 3,4192

Upper Bound 4,8941


Median 4,1650

Variance ,494


Minimum 3,29

Maximum 4,98

Range 1,69


Skewness -,045 ,845

Kurtosis -1,847 1,741

dosis 2

Mean 3,0900 ,55441


Mean

Lower Bound 1,6648

Upper Bound 4,5152


Median 3,3550

Variance 1,844

Std. Deviation 1,35803

Minimum 1,45

Maximum 4,46

Range 3,01


Skewness -,315 ,845

Kurtosis -2,418 1,741

dosis 3 Mean 3,3083 ,28824


Mean

Lower Bound 2,5674

Upper Bound 4,0493


Median 3,4250

Variance ,498


Minimum 2,29

Maximum 4,15

Range 1,86


Skewness -,353 ,845

Kurtosis -1,340 1,741

Lampiran 3 Test of Normality Sebelum transformasi

Tests of Normality

dosis Kolmogorov-Smirnova Shapiro-Wilk

Statistic Df Sig. Statistic df Sig.

agnor

kontrol ,147 6 ,200* ,989 6 ,986

dosis 1 ,182 6 ,200* ,920 6 ,507

dosis 2 ,244 6 ,200* ,856 6 ,175

dosis 3 ,243 6 ,200* ,942 6 ,672

*. This is a lower bound of the true significance.

a. Lilliefors Significance Correction

Lampiran 4 Homogenity Test Sebelum transformasi

Test of Homogeneity of Variance

Levene Statistic df1 df2 Sig.

agnor

Based on Mean 3,359 3 20 ,039

Based on Median 2,900 3 20 ,060

Based on Median and with

adjusted df

2,900 3 16,676 ,066

Based on trimmed mean 3,356 3 20 ,039

Lampiran 5 Test of Normality Setelah transformasi

Tests of Normality

dosis Kolmogorov-Smirnova Shapiro-Wilk

Statistic Df Sig. Statistic Df Sig.

trans_agnor

kontrol ,163 6 ,200* ,976 6 ,930

dosis 1 ,186 6 ,200* ,917 6 ,482

dosis 2 ,223 6 ,200* ,860 6 ,190

dosis 3 ,234 6 ,200* ,944 6 ,689

*. This is a lower bound of the true significance.

a. Lilliefors Significance Correction

Lampiran 6 Homogenity Test Setelah transformasi

Test of Homogeneity of Variance

Levene Statistic df1 df2 Sig.

trans_agnor

Based on Mean 1,195 3 20 ,337

Based on Median 1,171 3 20 ,346

Based on Median and with

adjusted df

1,171 3 10,086 ,369

Based on trimmed mean 1,194 3 20 ,338

Lampiran 7 One Way Anova Test

ANOVA

trans_agnor

Sum of Squares Df Mean Square F Sig.

Between Groups 3133,144 3 1044,381 19,659 ,000

Within Groups 1062,493 20 53,125

Total 4195,637 23

Lampiran 8 Post Hoc Bonferroni

Multiple Comparisons

Dependent Variable: trans_agnor

Bonferroni

(I) dosis (J) dosis Mean Difference

(I-J)

Std. Error Sig. 95% Confidence Interval

Lower Bound Upper Bound

kontrol

dosis 1 21,36053* 4,20811 ,000 9,0429 33,6782

dosis 2 27,96493* 4,20811 ,000 15,6473 40,2826

dosis 3 ,68942* 4,20811 ,000 15,3718 40,0071

dosis 1

kontrol -21,36053* 4,20811 ,000 -33,6782 -9,0429

dosis 2 6,60440 4,20811 ,793 -5,7133 18,9221

dosis 3 6,32888 4,20811 ,889 -5,9888 18,6465

dosis 2

kontrol -27,96493* 4,20811 ,000 -40,2826 -15,6473

dosis 1 -6,60440 4,20811 ,793 -18,9221 5,7133

dosis 3 -,27552 4,20811 1,000 -12,5932 12,0421

dosis 3

kotrol -27,68942* 4,20811 ,000 -40,0071 -15,3718

dosis 1 -6,32888 4,20811 ,889 -18,6465 5,9888

dosis 2 ,27552 4,20811 1,000 -12,0421 12,5932

*. The mean difference is significant at the 0.05 level.

Lampiran 9 Dokumentasi Penelitian

Gambar 1. Mencit donor

Gambar 2. Anastesi mencit donor

Gambar 3. Persiapan pembedahan mencit donor

Gambar 4. Pembedahan mencit donor

Gambar 5. Tumor

Gambar 6. Pembuatan bubur tumor

Gambar 7. Transplantasi bubur tumor ke mencit resipien

Gambar 8. Pemberian ekstrak Sarang Semut dan Methotrexate melalui sonde

Gambar 9. Preparat kelompok Kontrol, perbesaran 1000x dengan pengecatan AgNOR

Bintik AgNOR

Bintik AgNOR

Gambar 10. Preparat kelompok dosis 1, perbesaran 1000x dengan PengecetanAgNOR

Gambar 11. Preparat kelompok dosis 2, perbesaran 1000x dengan pengecatan AgNOR

Bintik AgNOR

Gambar 12. Preparat kelompok dosis 3, perbesaran 1000x dengan pengecatan AgNOR

Lampiran 10. Surat Ijin Penelitian

Bintik AgNOR

Lampiran 11. Surat Keterangan Penelitian dari Departemen Patologi Anatomik FK UI

Lampiran 12. Surat Ijin Penelitian Laboratorium Patologi Anatomi FK

UNISSULA

Lampiran 13.Ethical Clearance

LAMPIRAN semangat

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