LAMPIRAN G

PERHITUNGAN DOSIS UJI KELOMPOK PERLAKUAN DOSIS

1, perlakuan awal

- Dosis 400 mg/ kg BB

C = 400 = 61 mg = 0,061 g

C =

= 0,0203 g/ml x 5 ml = 0,1015 g dalam 5 ml aquadest,

- Dosis 600 mg/ kg BB

C = 600 = 106,2 mg = 0,1062 g

C =

= 0,0354 g/ml x 5 ml = 0,177 g dalam 5 ml aquadest,

- Dosis 900 mg/ kg BB

C = 900 = 132,3 mg = 0,1323 g

C =

= 0,0354 g/ml x 5 ml = 0,177 g dalam 5 ml aquadest,

Tabel volume pemberian minggu ke-0

Dosis 400 mg/kg BBDosis 600 mg/kg BBDosis 900 mg/kg BB

Berat badan (g)Volume (ml)Berat badan (g)Volume (ml)Berat badan (g)Volume (ml)

290,73240,60250,63

260,65260,65220,55

280,70300,75250,63

250,63320,80250,63

240,60310,78300,75

2, Perlakuan minggu ke-1

- Dosis 400 mg/ kg BB

C = 400 = 61,2 mg = 0,0612 g

C =

= 0,0204 g/ml x 5 ml = 0,102 g dalam 5 ml aquadest,

- Dosis 600 mg/ kg BB

C = 600 = 81 mg = 0,081 g

C =

= 0,027 g/ml x 5 ml = 0,135 g dalam 5 ml aquadest,

- Dosis 900 mg/ kg BB

C = 900 = 127,8 mg = 0,1278 g

C =

= 0,0426 g/ml x 5 ml = 0,213 g dalam 5 ml aquadest,

Tabel volume pemberian minggu ke 1

Dosis 400 mg/kg BBDosis 600 mg/kg BBDosis 900 mg/kg BB

Berat badan (g)Volume (ml)Berat badan (g)Volume (ml)Berat badan (g)Volume (ml)

300,75200,50200,50

280,70200,50240,60

270,68220,55240,60

260,65250,63250,63

190,48240,60270,68

3, Perlakuan minggu ke-2

- Dosis 400 mg/ kg BB

C = 400 = 58,4 mg = 0,0584 g

C =

= 0,0292 g/ml x 5 ml = 0,146 g dalam 5 ml aquadest,

- Dosis 600 mg/ kg BB

C = 600 = 90 mg = 0,09 g

C =

= 0,036 g/ml x 5 ml = 0,18 g dalam 5 ml aquadest,

- Dosis 900 mg/ kg BB

C = 900 = 144 mg = 0,144 g

C =

= 0,0576 g/ml x 5 ml = 0,288 g dalam 5 ml aquadest,

Tabel volume pemberian minggu ke-2

Dosis 400 mg/kg BBDosis 600 mg/kg BBDosis 900 mg/kg BB

Berat badan (g)Volume (ml)Berat badan (g)Volume (ml)Berat badan (g)Volume (ml)

250,63270,68220,55

300,75180,45290,73

300,75200,50230,58

290,73260,63300,75

320,80270,68310,78

4, Perlakuan minggu ke-3

- Dosis 400 mg/ kg BB

C = 400 = 56,8 mg = 0,0568 g

C =

= 0,02272 g/ml x 5 ml = 0,1136 g dalam 5 ml aquadest,

- Dosis 600 mg/ kg BB

C = 600 = 79,2 mg = 0,0792 g

C =

= 0,0792 g/ml x 5 ml = 0,1584 g dalam 5 ml aquadest,

- Dosis 900 mg/ kg BB

C = 900 = 152,1 mg = 0,1521 g

C =

= 0,0507 g/ml x 5 ml = 0,2535 g dalam 5 ml aquadest,

Tabel volume pemberian minggu ke-3

Dosis 400 mg/kg BBDosis 600 mg/kg BBDosis 900 mg/kg BB

Berat badan (g)Volume (ml)Berat badan (g)Volume (ml)Berat badan (g)Volume (ml)

250,63250,63230,58

280,70220,55300,75

300,75260,65240,60

290,73270,68320,80

300,75320,80330,83

5, Perlakuan minggu ke-4

- Dosis 400 mg/ kg BB

C = 400 = 57,6 mg = 0,0576 g

C =

= 0,0576 g/ml x 5 ml = 0,1152 g dalam 5 ml aquadest,

- Dosis 600 mg/ kg BB

C = 600 = 75,6 mg = 0,0756 g

C =

= 0,03024 g/ml x 5 ml = 0,1512 g dalam 5 ml aquadest,

- Dosis 900 mg/ kg BB

C = 900 = 141,3 mg = 0,1413 g

C =

= 0,0471 g/ml x 5 ml = 0,2355 g dalam 5 ml aquadest,

Tabel volume pemberian minggu ke-4

Dosis 400 mg/kg BBDosis 600 mg/kg BBDosis 900 mg/kg BB

Berat badan (g)Volume (ml)Berat badan (g)Volume (ml)Berat badan (g)Volume (ml)

250,63240,60250,63

310,78220,55280,70

320,80260,65200,50

260,65250,63310,78

300,75290,73270,68

PERHITUNGAN DOSIS UJI KELOMPOK PERLAKUAN SATELIT

1, Perlakuan awal

- Dosis 400 mg/ kg BB

C = 400 = 55,2 mg = 0,0552 g

C =

= 0,02208 g/ml x 5 ml = 0,1104 g dalam 5 ml aquadest,

- Dosis 600 mg/ kg BB

C = 600 = 72,6 mg = 0,0726 g

C =

= 0,02904 g/ml x 5 ml = 0,1452 g dalam 5 ml aquadest,

- Dosis 900 mg/ kg BB

C = 900 = 111,6 mg = 0,1116 g

C =

= 0,04464 g/ml x 5 ml = 0,2232 g dalam 5 ml aquadest,

Tabel volume pemberian minggu ke-0

Dosis 400 mg/kg BBDosis 600 mg/kg BBDosis 900 mg/kg BB

Berat badan (g)Volume (ml)Berat badan (g)Volume (ml)Berat badan (g)Volume (ml)

240,60220,65280,70

280,70200,50290,73

350,88280,70220,55

270,68250,63240,60

240,60260,65210,53

2, Perlakuan minggu ke-1

- Dosis 400 mg/ kg BB

C = 400 = 52 mg = 0,052 g

C =

= 0,0208 g/ml x 5 ml = 0,104 g dalam 5 ml aquadest,

- Dosis 600 mg/ kg BB

C = 600 = 67,2 mg = 0,0672 g

C =

= 0,02688 g/ml x 5 ml = 0,1344 g dalam 5 ml aquadest,

- Dosis 900 mg/ kg BB

C = 900 = 107,1 mg = 0,1071 g

C =

= 0,04284 g/ml x 5 ml = 0,2142 g dalam 5 ml aquadest,

Tabel volume pemberian minggu ke-1

Dosis 400 mg/kg BBDosis 600 mg/kg BBDosis 900 mg/kg BB

Berat badan (g)Volume (ml)Berat badan (g)Volume (ml)Berat badan (g)Volume (ml)

250,63200,50240,60

250,63190,48250,63

320,80240,60220,55

250,63240,60230,58

230,58250,63250,63

3, Perlakuan minggu ke-2

- Dosis 400 mg/ kg BB

C = 400 = 56 mg = 0,056 g

C =

= 0,0224 g/ml x 5 ml = 0,112 g dalam 5 ml aquadest,

- Dosis 600 mg/ kg BB

C = 600 = 72 mg = 0,072 g

C =

= 0,0288 g/ml x 5 ml = 0,144 g dalam 5 ml aquadest,

- Dosis 900 mg/ kg BB

C = 900 = 94,5 mg = 0,0945 g

C =

= 0,04725 g/ml x 5 ml = 0,23625 g dalam 5 ml aquadest,

Tabel volume pemberian

Dosis 400 mg/kg BBDosis 600 mg/kg BBDosis 900 mg/kg BB

Berat badan (g)Volume (ml)Berat badan (g)Volume (ml)Berat badan (g)Volume (ml)

260,65200,50310,78

260,65290,73250,63

290,73200,50200,50

340,85250,6300

250,63260,65290,73

4, Perlakuan minggu ke-3

- Dosis 400 mg/ kg BB

C = 400 = 58,8 mg = 0,0588 g

C =

= 0,02352 g/ml x 5 ml = 0,1176 g dalam 5 ml aquadest,

- Dosis 600 mg/ kg BB

C = 600 = 68,4 mg = 0,0684 g

C =

= 0,02736 g/ml x 5 ml = 0,1368 g dalam 5 ml aquadest,

- Dosis 900 mg/ kg BB

C = 900 = 99,9 mg = 0,0999 g

C =

= 0,04995 g/ml x 5 ml = 0,24975 g dalam 5 ml aquadest,

Tabel volume pemberian minggu ke-3

Dosis 400 mg/kg BBDosis 600 mg/kg BBDosis 900 mg/kg BB

Berat badan (g)Volume (ml)Berat badan (g)Volume (ml)Berat badan (g)Volume (ml)

270,53160,40230,58

270,50230,58230,58

300,73200,50200,50

330,85240,6000

300,73260,65250,63

5, Perlakuan minggu ke-4

- Dosis 400 mg/ kg BB

C = 400 = 53,2 mg = 0,0532 g

C =

= 0,02128 g/ml x 5 ml = 0,1064 g dalam 5 ml aquadest,

- Dosis 600 mg/ kg BB

C = 600 = 61,8 mg = 0,0618 g

C =

= 0,02472 g/ml x 5 ml = 0,1236 g dalam 5 ml aquadest,

- Dosis 900 mg/ kg BB

C = 900 = 81,9 mg = 0,0819 g

C =

= 0,04095 g/ml x 5 ml = 0,20475 g dalam 5 ml aquadest,

Tabel volume pemberian minggu ke-4

Dosis 400 mg/kg BBDosis 600 mg/kg BBDosis 900 mg/kg BB

Berat badan (g)Volume (ml)Berat badan (g)Volume (ml)Berat badan (g)Volume (ml)

210,53160,40230,58

200,50230,58230,58

290,73200,50200,50

340,85240,6000

290,73260,65250,63

LAMPIRAN H

DATA PENIMBANGAN BERAT BADAN

BERAT BADAN KELOMPOK PERLAKUAN

Nomor kelompok mencitBerat badan

Perlakuan awalMinggu ke-1Minggu ke-2Minggu ke-3Minggu ke-4Sebelum bedah

Kontrol

1222425232323

2232732323233

3292825262726

4232230323334

5282527272729

Dosis 400 mg/ kg BB

1293025252526

226283028310

3282730303232

4252629292627

6222332303030

Dosis 600 mg/ kg BB

7242027252428

8262018222220

10322526262625

11312427272528

12342432322933

Dosis 900 mg/ kg BB

13252022232527

14222425302832

15252423242024

16252530323133

17302731332723

BERAT BADAN KELOMPOK SATELIT

Nomor kelompok mencitBerat badan

Perlakuan awalMinggu ke-1Minggu ke-2Minggu ke-3Minggu ke-4Sebelum bedah

Kontrol

1222425232323

2232732323233

3292825262726

4232230323334

5282527272729

Dosis 400 mg/ kg BB

19242526272129

2028252627200

2135322930290

22272534333437

23242325302930

Dosis 600 mg/ kg BB

2422202019160

25201929262325

26282420212024

27252425262426

2826252622260

Dosis 900 mg/ kg BB

29282431312325

20292525262325

31222220242030

3321252930250

34252529302525

LAMPIRAN I

DATA PENIMBANGAN BERAT ORGAN

Tabel berat organ mencit kelompok perlakuan

No, BBJantungParu-paruLimphaHeparGinjalOvarium

Kontrol negatif

1230,10500,31430,10961,05020,45430,3130

2330,10370,21360,18831,14880,36370,2172

3260,10250,20010,19701,33560,43730,0840

4340,15060,40000,16371,59670,67490,2595

5290,12200,19640,14541,45050,50330,1316

Dosis 400 mg/kg BB

1260,09490,16530,17921,22460,24470,1147

2310,12500,35050,50431,46000,35430,1563

3320,13460,26830,23871,80340,36460,1288

4270,14340,24880,22161,92140,47710,2143

6300,12850,31870,17481,58810,40460,2004

Dosis 600 mg/ Kg BB

7280,12720,21060,15241,49210,33840,0971

8200,10930,15460,14311,04120,28880,1498

10250,12560,30170,22381,65390,39640,2325

11280,10110,31700,19321,45900,33870,0892

12330,15280,34490,23551,64080,33770,1671

Dosis 900 mg/ Kg BB

13270,11050,33930,20851,63550,31120,1544

14320,10520,18700,15811,42870,30970,1414

15240,12000,16950,17041,24210,25530,1706

16330,14320,24030,27411,71460,40950,1023

17230,13010,20760,15041,58040,29550,1295

Tabel berat organ mencit kelompok satelit

No,BBJantungParu-paruLimphaHeparGinjalOvarium

Kontrol negatif

1230,10500,31430,10961,05020,45430,3130

2330,10370,21360,18831,14880,36370,2172

3260,10250,20010,19701,33560,43730,0840

4340,15060,40000,16371,59670,67490,2595

5290,12200,19640,14541,45050,50330,1316

Dosis 400 mg/kg BB

19290,15150,27220,19581,94440,39490,1322

20200,10850,25280,08751,00850,31650,0495

21290,16300,35900,12501,70900,21200,2560

22370,10650,26650,35471,91080,42320,0871

23300,15930,26240,11061,45770,34500,1859

Dosis 600 mg/ Kg BB

24160,11620,17660,19700,91550,29190,0560

25250,11560,30380,19051,26520,39160,1728

26240,09450,24190,18861,56850,28560,0828

27260,10600,29940,09721,22690,34290,2314

28260,38170,28270,12311,14880,33640,1103

Dosis 900 mg/ Kg BB

29250,12000,27070,11381,49880,29190,0560

20250,09360,17660,13981,35880,26540,1284

31300,16890,24220,26451,97010,40630,2075

33250,26760,23240,12310,91550,34300,1103

34250,38170,29270,25241,22560,47060,5064

LAMPIRAN J

DATA PERHITUNGAN INDEKS ORGAN

Tabel indeks organ mencit kelompok perlakuan

No, BBJantungParu-paruLimphaHeparGinjalOvarium

Kontrol negatif

1230,45651,36650,47654,56611,97521,3609

2330,31420,64730,57063,48121,10210,6582

3260,39420,76960,75775,13691,68190,3231

4340,44291,17650,48154,69621,98500,7632

5290,42070,67720,50145,00171,73550,4538

Dosis 400 mg/kg BB

1260,36500,63580,68924,71000,94120,4412

2310,40321,13061,62684,70971,14290,5042

3320,42060,83840,74595,63561,13940,4025

4270,53110,92150,82077,11631,76700,7937

6300,42831,06230,58275,29371,34870,6680

Dosis 600 mg/ Kg BB

7280,45430,75210,54435,32891,20860,3468

8200,54650,77300,71555,20601,44400,7490

10250,50241,20680,89526,61561,58560,9300

11280,36111,13210,69005,21071,20960,3186

12330,46301,04520,71364,97211,02330,5064

Dosis 900 mg/ Kg BB

13270,40931,25670,77226,05741,15260,5719

14320,32880,58440,49414,46470,96780,4419

15240,50000,70630,71005,17541,06380,7108

16330,43390,72820,83065,19581,24090,3100

17230,56570,90260,65396,87131,28480,5630

Tabel indeks organ mencit kelompok satelit

No, BBJantungParu-paruLimphaHeparGinjalOvarium

Kontrol negatif

1230,45651,36650,47654,56611,97521,3609

2330,31420,64730,57063,48121,10210,6582

3260,39420,76960,75775,13691,68190,3231

4340,44291,17650,48154,69621,98500,7632

5290,42070,67720,50145,00171,73550,4538

Dosis 400 mg/kg BB

19290,52240,93860,67526,70481,36170,4559

20200,54251,26400,43755,04251,58250,2475

21290,56211,23790,43105,89310,73100,8828

22370,28780,72030,95865,16431,14380,2354

23300,53100,87470,36874,85901,15000,6197

Dosis 600 mg/ Kg BB

24160,72631,10381,23135,72191,82440,3500

25250,46241,21520,76205,06081,56640,6912

26240,39381,00790,78586,53541,19000,3450

27260,40771,15150,37384,71881,31880,8900

28261,46811,08730,47254,41851,29380,4242

Dosis 900 mg/ Kg BB

29250,48001,08280,45525,99521,16760,2240

20250,37440,70640,55925,43521,06160,5136

31300,56300,80730,88176,56701,35430,6917

33251,07040,92960,49243,66201,37200,4412

34251,52681,17081,00964,90241,88242,0256

LAMPIRAN L

ANALISIS DATA INDEKS ORGAN

BERAT BADAN DAN JUMLAH JENGUKAN

1. Tabel analisis data indeks organ ginjal

Test of Homogeneity of Variances

organ_ginjal

Levene Statisticdf1df2Sig.

0.4116280.865

ANOVA

organ_ginjal

Sum of SquaresdfMean SquareFSig.

Between Groups1.0256.1712.144.080

Within Groups2.23128.080

Total3.25734

2. Tabel analisis data indeks organ jantung

Test of Homogeneity of Variances

organ_jantung

Levene Statisticdf1df2Sig.

6.357628.000

ANOVA

organ_jantung

Sum of SquaresdfMean SquareFSig.

Between Groups.6946.1161.705.157

Within Groups1.89828.068

Total2.59134

Kruskal-Wallis Test

Ranks

indeks organNMean Rank

organ_jantungkontrol negatif511.40

p1 400 mg513.20

p2 600 mg518.40

p3 900 mg516.40

s1 400 mg521.20

s2 600 mg520.40

s3 900 mg525.00

Total35

Test Statisticsa,b

organ_jantung

Chi-Square6.396

df6

Asymp. Sig..380

a. Kruskal Wallis Test

b. Grouping Variable: indeks organ

3. Analisis data indeks organ paru-paru

Test of Homogeneity of Variances

organ_paru_paru

Levene Statisticdf1df2Sig.

2.307628.062

ANOVA

organ_paru_paru

Sum of SquaresdfMean SquareFSig.

Between Groups.2246.037.741.621

Within Groups1.41328.050

Total1.63734

4. Analisis data indeks organ limpa

Test of Homogeneity of Variances

organ_limpha

Levene Statisticdf1df2Sig.

1.798628.136

ANOVA

organ_limpha

Sum of SquaresdfMean SquareFSig.

Between Groups.3706.062.946.479

Within Groups1.82528.065

Total2.19534

5. Analisis data indeks organ hepar

Test of Homogeneity of Variances

organ_hepar

Levene Statisticdf1df2Sig.

.446628.842

ANOVA

organ_hepar

Sum of SquaresdfMean SquareFSig.

Between Groups3.5246.587.785.589

Within Groups20.95128.748

Total24.47534

6. Analisis data indeks organ ovarium

Test of Homogeneity of Variances

organ_ovarium

Levene Statisticdf1df2Sig.

1.818628.132

ANOVA

organ_ovarium

Sum of SquaresdfMean SquareFSig.

Between Groups.3476.058.437.848

Within Groups3.70628.132

Total4.05334

7. Analisis data berat badan mencit

Test of Homogeneity of Variances

Levene Statisticdf1df2Sig.

minggu_0.549628.767

minggu_1.616628.716

minggu_2.429628.853

minggu_31.365628.263

minggu_41.850628.125

sebelum_bedah4.578628.002

ANOVA

Sum of SquaresdfMean SquareFSig.

minggu_0Between Groups98.343616.3901.317.282

Within Groups348.4002812.443

Total446.74334

minggu_1Between Groups81.886613.6482.090.086

Within Groups182.800286.529

Total264.68634

minggu_2Between Groups85.086614.181.929.489

Within Groups427.2002815.257

Total512.28634

minggu_3Between Groups145.771624.2952.137.080

Within Groups318.4002811.371

Total464.17134

minggu_4Between Groups198.286633.0482.212.072

Within Groups418.4002814.943

Total616.68634

sebelum_bedahBetween Groups779.1436129.8571.029.427

Within Groups3532.40028126.157

Total4311.54334

Kruskal-Wallis Test

Ranks

perubahan_berat_badanNMean Rank

sebelum_bedah1523.40

2519.70

3519.60

4520.20

5518.70

659.90

7514.50

Total35

Test Statisticsa,b

sebelum_bedah

Chi-Square5.667

df6

Asymp. Sig..461

a. Kruskal Wallis Test

b. Grouping Variable: perubahan_berat_badan

Analisis jengukan mencit

Test of Homogeneity of Variances

jengukan_mencit

Levene Statisticdf1df2Sig.

2.4106189.029

ANOVA

jengukan_mencit

Sum of SquaresdfMean SquareFSig.

Between Groups242.214640.3693.604.002

Within Groups2116.78618911.200

Total2359.000195

Multiple Comparisons

jengukan_mencit

Tukey

jumlah_jengukan jumlah_jengukanMean Difference (I-J)Std. ErrorSig.95% Confidence Interval

Lower BoundUpper Bound

kontrol negatifp1-.821.894.969-3.491.84

p2-2.286.894.146-4.95.38

p3-2.929*.894.021-5.59-.26

s1-.250.8941.000-2.922.42

s2-.357.8941.000-3.022.31

s3.143.8941.000-2.522.81

p1kontrol negatif.821.894.969-1.843.49

p2-1.464.894.658-4.131.20

p3-2.107.894.224-4.77.56

s1.571.894.995-2.093.24

s2.464.894.999-2.203.13

s3.964.894.934-1.703.63

p2kontrol negatif2.286.894.146-.384.95

p11.464.894.658-1.204.13

p3-.643.894.991-3.312.02

s12.036.894.261-.634.70

s21.929.894.325-.744.59

s32.429.894.100-.245.09

p3kontrol negatif2.929*.894.021.265.59

p12.107.894.224-.564.77

p2.643.894.991-2.023.31

s12.679*.894.048.015.34

s22.571.894.067-.095.24

s33.071*.894.013.415.74

s1kontrol negatif.250.8941.000-2.422.92

p1-.571.894.995-3.242.09

p2-2.036.894.261-4.70.63

p3-2.679*.894.048-5.34-.01

s2-.107.8941.000-2.772.56

s3.393.894.999-2.273.06

s2kontrol negatif.357.8941.000-2.313.02

p1-.464.894.999-3.132.20

p2-1.929.894.325-4.59.74

p3-2.571.894.067-5.24.09

s1.107.8941.000-2.562.77

s3.500.894.998-2.173.17

s3kontrol negatif-.143.8941.000-2.812.52

p1-.964.894.934-3.631.70

p2-2.429.894.100-5.09.24

p3-3.071*.894.013-5.74-.41

s1-.393.894.999-3.062.27

s2-.500.894.998-3.172.17

*. The mean difference is significant at the 0.05 level.

LAMPIRAN M

TABEL CHI-SQUARE