Lampiran 1. Hasil Determinasi Buah Cabai Paprika Meraheprints.unwahas.ac.id/1031/6/lampiran.pdf ·...
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Transcript of Lampiran 1. Hasil Determinasi Buah Cabai Paprika Meraheprints.unwahas.ac.id/1031/6/lampiran.pdf ·...
39
DAFTAR PUSTAKA
Lampiran 1. Hasil Determinasi Buah Cabai Paprika Merah
40
Lanjutan lampiran 1....
41
Lanjutan lampiran 1 ....
42
Lampiran 2. Surat Keterangan Telah Melakukan Penelitian di
Laboratorium Kimia Farmasi Universitas Wahid Hasyim
Semarang
43
Lampiran 3. Data Perhitungan dan Penimbangan vitamin C
a. Data Penimbangan vitamin C
Keterangan Hasil Penimbangan
Berat cawan 25577,2mg
Berat cawan + vitamin C 25589,1 mg
Berat cawan + sisa 25578,8 mg
Berat vitamin C 10,3 mg
b. Pembuatan Larutan Stok Vitamin C 10 ppm
Vitamin C 200 ppm = 10.000 µg ÷ 50 mL
Vitamin C 10 ppm = (200 ppm x 0.5 mL) ÷ 10 ppm = 10 mL
Vitamin C 200 ppm dipipet 0.5 mL dimasukkan dalam labu takar 10 mL
kemudian ad dengan aquadest sampai tanda batas.
c. Pembuatan Kurva baku vitamin C
1 .Pembuatan larutan konsentrasi 0.5 ppm
C1 X V1 = C2 X V2
10 ppm X V1 = 0.5 ppm X 5 ml
V1 = 0.25 ml = 250 µL
Sebanyak 250 µL larutan stok vitamin C 10 ppm diencerkan dalam
aquadest ad 5 ml dalam labu takar.
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Lanjutan Lampiran 3...
2. Pembuatan larutan konsentrasi 1 ppm
C1 X V1 = C2 X V2
10 ppm X V1 = 1 ppm X 5 ml
V1 = 500 µL
Sebanyak 500 µL larutan stok vitamin C 10 ppm diencerkan dalam
aquadest ad 5 ml dalam labu takar.
3. Pembuatan larutan konsentrasi 1,5 ppm sebanyak 10 ml
C1 X V1 = C2 X V2
10 ppm X V1 = 1.5 ppm X 5 ml
V1 = 750 µL
Sebanyak 750 µL larutan stok vitamin C 10 ppm diencerkan dalam
aquadest ad 5 ml dalam labu takar.
4. Pembuatan larutan konsentrasi 2 ppm sebanyak 10 ml
C1 X V1 = C2 X V2
10 ppm X V1 = 2 ppm X 5 ml
V1 = 1000 µL
Sebanyak 1000 µL larutan stok vitamin C 10 ppm diencerkan dalam
aquadest ad 5 ml dalam labu takar.
5.Pembuatan larutan konsentrasi 2,5 ppm sebanyak 10 ml
C1 X V1 = C2 X V2
10 ppm X V1 = 2.5 ppm X 5 ml
V1 = 1250 µL
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Lanjutan Lampiran 3...
Sebanyak 1250 µL larutan stok vitamin C 10 ppm diencerkan dalam
aquadest ad 5 ml dalam labu takar.
d. Data Penimbangan Buah Cabai Paprika Merah
Keterangan Hasil Penimbangan
Berat cawan 50,9709 g
Berat cawan + paprika merah 56,1103 g
Berat cawan + sisa 51,0170 g
Berat paprika merah 5,0933 g
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Lampiran 4. Data Perhitungan Kadar vitamin C
Persamaan kurva baku Y= 0,13901 x - 0,00808
Perhitungan:
Kadar vitamin C 0 hari suhu kamar ( Faktor Pengenceran = 10x)
(Y= 0,13901 x - 0,00808) X FP
(0,449= 0,13901X - 0,00808) X 10
X=( 0,449 + 0,00808 ) X 10
0,13901
X =32,8810 mg/ 5 g
X= 32,8810 mg X 100 g
5 g
X =657,62 mg =0,6576 g/100 g = 0,658 g/100 g
Perlakuan Absorbansi
(y)
Kadar Vitamin
C(mg)
Kadar vitamin
C (%b/b)
kadar vitamin C
(%b/b)
rata-rata
0 Hari Suhu kamar 0.449 32.8810 0.658
0.65 0.445 32.5933 0.652
0.442 32.3775 0.647
3 Hari
0°C
0.767 55.575 1.115
0.86 0.510 37.269 0.745
0.681 35.701 0.714
10°C
0.771 56.044 1.121
0.95 0.505 36.909 0.734
0.681 49.570 0.991
Suhu kamar
0.773 56.188 1.124
0.95 0.504 36.837 0.737
0.680 49.498 0.989
7 Hari
0°C
0.431 31.586 0.632
0.61 0.386 28.349 0.567
0.432 31.658 0.633
10°C
0.450 32.953 0.659
0.64 0.410 30.075 0.602
0.448 32.809 0.656
Suhu kamar 0.364 26.766 0.535
0.52 0.331 24.392 0.488
0.365 26.838 0.537
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Lampiran 5. Data Perhitungan dan penimbangan DPPH
a. Pembuatan larutan DPPH
Molaritas DPPH yang dibutuhkan = 0,5 mM
BM DPPH = 394,32 g/mol
Volume larutan = 100 ml
Penimbangan DPPH = BM DPPH x Vol larutan x Molaritas DPPH
= (394,32 g/mol x 100 ml x 0,5 mM) / 1000
= 19,716 mg = 19,7 mg
Cara pembuatan larutan DPPH :
Ditimbang DPPH 19,7 mg dilarutkan dengan metanol p.a 100 ml dan divortek
homogen. Didapat konsentrasi 0,5 mM sebanyak 100 ml, kemudian diencerkan 10
x yaitu diambil 10 ml larutan DPPH 0,5 mM dan ditambahkan metanol p.a 90 ml
sehingga diperoleh larutan DPPH 100 ml konsentrasi 0,05 mM.
b. Data Penimbangan DPPH
Keterangan Hasil Penimbangan
Berat botol timbang 10 710,8 mg
Berat botol timbang + DPPH 10730,8 mg
Berat botol timbang + sisa 10711,1mg
Berat DPPH 19,7 mg
48
Lampiran 6. Data Perhitungan aktivitas antioksidan
Aktivitas antioksidan dihitung menggunakan rumus :
a.
Keterangan :
Abs.kontrol : Serapan radikal DPPH 0,05 mM kontrol pada panjang gelombang maksimum.
Abs.Sampel : Serapan radikal DPPH 0,05 mM sampel pada panjang gelombang maksimum.
Perlakuan Absorbansi
Aktivitas
Antioksidan
(% inhibisi)
Aktivitas
Antioksidan
Rata-rata
(% inhibisi)
0 Hari Suhu kamar
0.600 36.5079
35.03 0.610 35.4497
0.632 33.1217
3 Hari
0°C
0.676 28.4656
29.21 0.666 29.5238
0.665 29.6296
10°C
0.624 33.9682
33.93 0.630 33.3333
0.619 34.4973
Suhu kamar
0.695 26.4550
26.17 0.696 26.3492
0.702 25.7143
7 Hari
0°C
0.688 27.1958
27.16 0.692 26.7725
0.685 27.5132
10°C
0.646 31.6402
31.46 0.648 31.4286
0.649 31.3227
Suhu kamar
0.720 23.8095
24.02 0.715 24.3386
0.719 23.9153
% Inhibisi = Abs. kontrol – Abs.Sampel x 100 %
Abs.kontrol
49
Lampiran 7. Analisis Data Secara Statistik
STASTISTIK PENGARUH SUHU DAN LAMA PENYIMPANAN TERHADAP
KADAR VITAMIN C
One-Sample Kolmogorov-Smirnov Test
kadar vitamin c % inhibisi
N 21 21
Normal Parametersa Mean .73981 42.41205
Std. Deviation .201810 8.059471
Most Extreme Differences Absolute .252 .138
Positive .252 .138
Negative -.130 -.097
Kolmogorov-Smirnov Z 1.153 .634
Asymp. Sig. (2-tailed) .140 .816
a. Test distribution is Normal.
Between-Subjects Factors
Value Label N
suhu 1 suhu kamar 9
2 suhu 0 6
3 suhu 10 6
lama penyimpanan 1 0 hari 3
2 3 hari 9
3 7 hari 9
50
Lanjutan lampiran 7 ....
Descriptive Statistics
Dependent Variable:kadar vitamin c
suhu
lama
penyimpanan Mean Std. Deviation N
suhu kamar 0 hari .65233 .005508 3
3 hari .95000 .196426 3
7 hari .52000 .027731 3
Total .70744 .214995 9
suhu 0 3 hari .85800 .223108 3
7 hari .61067 .037820 3
Total .73433 .197066 6
suhu 10 3 hari .94867 .196942 3
7 hari .63900 .032078 3
Total .79383 .211410 6
Total 0 hari .65233 .005508 3
3 hari .91889 .184044 9
7 hari .58989 .060870 9
Total .73981 .201810 21
51
Lanjutan lampiran 7 ....
Levene's Test of Equality of Error Variancesa
Dependent Variable:% inhibisi
F df1 df2 Sig.
.239 6 14 .956
Tests the null hypothesis that the error variance
of the dependent variable is equal across groups.
a. Design: Intercept + SUHU + LP + SUHU * LP
Tests of Between-Subjects Effects
Dependent Variable:kadar vitamin c
Source
Type III Sum of
Squares df Mean Square F Sig.
Corrected Model .554a 6 .092 4.954 .006
Intercept 10.112 1 10.112 542.785 .000
suhu .014 2 .007 .376 .693
lp .501 2 .250 13.440 .001
suhu * lp .026 2 .013 .694 .516
Error .261 14 .019
Total 12.308 21
Corrected Total .815 20
a. R Squared = ,680 (Adjusted R Squared = ,543)
52
Lanjutan lampiran 7 ....
Estimated Marginal Means
1. suhu
Dependent Variable:kadar vitamin c
suhu Mean Std. Error
95% Confidence Interval
Lower Bound Upper Bound
suhu kamar .707 .045 .610 .805
suhu 0 .734a .056 .615 .854
suhu 10 .794a .056 .674 .913
a. Based on modified population marginal mean.
2. lama penyimpanan
Dependent Variable:kadar vitamin c
lama
penyimp
anan Mean Std. Error
95% Confidence Interval
Lower Bound Upper Bound
0 hari .652a .079 .483 .821
3 hari .919 .045 .821 1.016
7 hari .590 .045 .492 .687
a. Based on modified population marginal mean.
53
Lanjutan lampiran 7 ....
Post Hoc Tests
suhu
Multiple Comparisons
kadar vitamin c
Tukey HSD
(I) suhu (J) suhu
Mean Difference
(I-J) Std. Error Sig.
95% Confidence Interval
Lower Bound Upper Bound
suhu kamar suhu 0 -.02689 .071936 .926 -.21517 .16139
suhu 10 -.08639 .071936 .472 -.27467 .10189
suhu 0 suhu kamar .02689 .071936 .926 -.16139 .21517
suhu 10 -.05950 .078802 .736 -.26575 .14675
suhu 10 suhu kamar .08639 .071936 .472 -.10189 .27467
suhu 0 .05950 .078802 .736 -.14675 .26575
Based on observed means.
The error term is Mean Square (Error) = ,019.
54
Lanjutan lampiran 7 ....
Homogeneous Subsets
kadar vitamin c
Tukey HSD
suhu N
Subset
1
suhu kamar 9 .70744
suhu 0 6 .73433
suhu 10 6 .79383
Sig. .493
Means for groups in homogeneous subsets are displayed.
Based on observed means.
The error term is Mean Square (Error) = .019
55
Lanjutan lampiran 7 ....
Multiple Comparisons
kadar vitamin c
Tukey HSD
(I) lama
penyim
panan
(J) lama
penyim
panan
Mean Difference
(I-J) Std. Error Sig.
95% Confidence Interval
Lower Bound Upper Bound
0 hari 3 hari -.26656* .090993 .028 -.50471 -.02840
7 hari .06244 .090993 .775 -.17571 .30060
3 hari 0 hari .26656* .090993 .028 .02840 .50471
7 hari .32900* .064342 .000 .16060 .49740
7 hari 0 hari -.06244 .090993 .775 -.30060 .17571
3 hari -.32900* .064342 .000 -.49740 -.16060
Based on observed means.
The error term is Mean Square (Error) = ,019.
*. The mean difference is significant at the ,05 level.
56
Lanjutan lampiran 7 ....
Homogeneous Subsets
kadar vitamin c
Tukey HSD
lama
penyimpanan N
Subset
1 2
7 hari 9 .58989
0 hari 3 .65233
3 hari 9 .91889
Sig. .738 1.000
Means for groups in homogeneous subsets are displayed.
Based on observed means.
The error term is Mean Square(Error) = ,019.
57
Lanjutan lampiran 7 ....
STASTISTIK PENGARUH SUHU DAN LAMA PENYIMPANAN
TERHADAP % INHIBISI
Between-Subjects Factors
Value Label N
suhu 1 suhu kamar 9
2 suhu 0 6
3 suhu 10 6
lama penyimpanan 1 0 hari 3
2 3 hari 9
3 7 hari 9
58
Lanjutan lampiran 7 ....
Descriptive Statistics
Dependent Variable:% inhibisi
suhu
lama
penyimpanan Mean Std. Deviation N
suhu kamar 0 hari 5.39810E1 4.069230 3
3 hari 3.54540E1 .733108 3
7 hari 3.16170E1 .485873 3
Total 4.03507E1 10.564002 9
suhu 0 3 hari 4.12633E1 1.277792 3
7 hari 3.72907E1 .699722 3
Total 3.92770E1 2.362958 6
suhu 10 3 hari 5.13693E1 1.334485 3
7 hari 4.59090E1 .344349 3
Total 4.86392E1 3.115179 6
Total 0 hari 5.39810E1 4.069230 3
3 hari 4.26956E1 7.045207 9
7 hari 3.82722E1 6.249159 9
Total 4.24120E1 8.059471 21
59
Lanjutan lampiran 7....
Levene's Test of Equality of Error Variancesa
Dependent Variable:% inhibisi
F df1 df2 Sig.
4.293 6 14 .120
Tests the null hypothesis that the error variance
of the dependent variable is equal across groups.
a. Design: Intercept + suhu + lp + suhu * lp
Tests of Between-Subjects Effects
Dependent Variable:% inhibisi
Source
Type III Sum of
Squares df Mean Square F Sig.
Corrected Model 1256.394a 6 209.399 68.643 .000
Intercept 37465.302 1 37465.302 1.228E4 .000
suhu 697.471 2 348.736 114.318 .000
lp 924.083 2 462.042 151.461 .000
suhu * lp 2.433 2 1.217 .399 .678
Error 42.708 14 3.051
Total 39073.519 21
Corrected Total 1299.101 20
a. R Squared = ,967 (Adjusted R Squared = ,953)
60
Lanjutan lampiran 7 ....
Estimated Marginal Means
1. suhu
Dependent Variable:% inhibisi
suhu Mean Std. Error
95% Confidence Interval
Lower Bound Upper Bound
suhu kamar 40.351 .582 39.102 41.599
suhu 0 39.277a .713 37.748 40.806
suhu 10 48.639a .713 47.110 50.168
a. Based on modified population marginal mean.
2. lama penyimpanan
Dependent Variable:% inhibisi
lama
penyimp
anan Mean Std. Error
95% Confidence Interval
Lower Bound Upper Bound
0 hari 53.981a 1.008 51.818 56.144
3 hari 42.696 .582 41.447 43.944
7 hari 38.272 .582 37.024 39.521
a. Based on modified population marginal mean.
61
Lanjutan lampiran 7 ....
Post Hoc Tests
suhu
Multiple Comparisons
% inhibisi
Tukey HSD
(I) suhu (J) suhu
Mean Difference
(I-J) Std. Error Sig.
95% Confidence Interval
Lower Bound Upper Bound
suhu kamar suhu 0 1.07367 .920532 .491 -1.33562 3.48296
suhu 10 -8.28850* .920532 .000 -10.69779 -5.87921
suhu 0 suhu
kamar -1.07367 .920532 .491 -3.48296 1.33562
suhu 10 -9.36217* 1.008392 .000 -12.00141 -6.72292
suhu 10 suhu
kamar 8.28850
* .920532 .000 5.87921 10.69779
suhu 0 9.36217* 1.008392 .000 6.72292 12.00141
Based on observed means.
The error term is Mean Square(Error) = 3,051.
*. The mean difference is significant at the ,05 level.
62
Lanjutan lampiran 7 ....
Homogeneous Subsets
% inhibisi
Tukey HSD
suhu N
Subset
1 2
suhu 0 6 3.92770E1
suhu kamar 9 4.03507E1
suhu 10 6 4.86392E1
Sig. .513 1.000
Means for groups in homogeneous subsets are displayed.
Based on observed means.
The error term is Mean Square(Error) = 3,051.
63
Lanjutan lampiran 7 ....
Lama Penyimpanan
Multiple Comparisons
% inhibisi
Tukey HSD
(I) lama
penyimp
anan
(J) lama
penyimpanan
Mean Difference
(I-J) Std. Error Sig.
95% Confidence Interval
Lower Bound Upper Bound
0 hari 3 hari 11.28544* 1.164391 .000 8.23791 14.33298
7 hari 15.70878* 1.164391 .000 12.66124 18.75631
3 hari 0 hari -11.28544* 1.164391 .000 -14.33298 -8.23791
7 hari 4.42333* .823349 .000 2.26840 6.57827
7 hari 0 hari -15.70878* 1.164391 .000 -18.75631 -12.66124
3 hari -4.42333* .823349 .000 -6.57827 -2.26840
Based on observed means.
The error term is Mean Square(Error) = 3,051.
*. The mean difference is significant at the ,05 level
64
Lanjutan lampiran 7 ....
Homogeneous Subsets
% inhibisi
Tukey HSD
lama
penyimpanan N
Subset
1 2 3
7 hari 9 3.82722E1
3 hari 9 4.26956E1
0 hari 3 5.39810E1
Sig. 1.000 1.000 1.000
Means for groups in homogeneous subsets are displayed.
Based on observed means.
The error term is Mean Square(Error) = 3,051.
65
Lampiran 8. Data gambar
a. Panjang Gelombang Vitamin C
66
Lanjutan Lampiran 8....
b. Data Panjang Gelombang DPPH
67
Lanjutan Lampiran 8....
c. Data Operating Time DPPH
68
Lanjutan lampiran 8 ....
d. Kurva Baku Vitamin C
69
Lanjutan lampiran 8 ....
e. Data- data Gambar Penelitian
Deret kurva baku vitamin C
Larutan paprika + DPPH
Paprika suhu 0°C, 10°C dan suhu kamar