Karbohidrat SMA
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KARBOHIDRAT * Reaksi monosakarida* Ikatan glikosida* Fungsi karbohidrat
http://114.141.57.234/wagiman/
KARBOHIDRAT :
Karbon dan hidrat
Senyawa yang rumus emphirisnya Cm(H2O)n
PEMBENTUKAN KARBOHIDRAT
Karbohidrat terjadi pada proses fotosintesis
mCO2 (g)+ nH2O (l) Cm(H2O)n(s) + mO2(g)klorofil
UV
PEMBAGIAN KARBOHIDRAT
Monosakarida glukosa,fruktosa, galaktosa (Karbohidrat paling sederhana) Disakarida maltosa, sukrosa dan laktosa (Karbohidrat yg terdiri dari 2 monosakarida) Oligosakarida : dekstrin (Karbohidrat yang terdiri dari beberapa monosakarida) Polisakarida amilum,glikogen dan selulosa (Karbohidrat yg terdiri dari banyak monosakarida)
Monosakarida
Memiliki atom karbon 3 sampai 7 Setiap atom karbon memiliki gugus
hidroksi dan keton atau aldehida. Setiap molekul monosakarida memiliki
1 gugus keton (ketosa) atau 1 gugus aldehida (aldosa)
Gugus aldehida selalu berada di atom C pertama
Gugus keton selalu berada di atom C kedua
Nama mono sakarida dengan akhiran osa
MonosakaridaAldosa (mis: glukosa) memiliki gugus aldehida pada salah satu ujungnya.
Ketosa (mis: fruktosa) biasanya memiliki gugus keton pada atom C2.
C
C OHH
C HHO
C OHH
C OHH
CH2OH
D-glucose
OH
C HHO
C OHH
C OHH
CH2OH
CH2OH
C O
D-fructose
Jika di sebelah kiri L
Notasi D vs L
Notasi D & L dilakukan karena adanya atom C dengan konfigurasi asimetris seperti pada gliseraldehida.
CH O
C
CH2OH
H OH
CH O
C
CH2OH
HO H
CH O
C
CH2OH
HO H
L-gliseraldehidaD-glyceraldehyde
L-gliseraldehida
CH O
C
CH2OH
H OH
D-gliseraldehida
Penampilan dalam bentuk gambar bagian bawah disebut Proyeksi Fischer.
Penamaan Gula
Untuk gula dengan atom C asimetrik lebih dari 1, notasi D atau L ditentukan oleh atom C asimetrik terjauh dari gugus aldehida atau keton.
Gula yang ditemui di alam adalah dalam bentuk isomer D.
O H O H C C H – C – OH HO – C – H HO – C – H H – C – OH H – C – OH HO – C – H H – C – OH HO – C – H CH2OH CH2OH
D-glukosa L-glukosa
Gula dalam bentuk D merupakan bayangan cermin dari gula dalam bentuk L. Kedua gula tersebut memiliki nama yang sama, misalnya D-glukosa & L-glukosa.
O H O H C C H – C – OH HO – C – H HO – C – H H – C – OH H – C – OH HO – C – H H – C – OH HO – C – H CH2OH CH2OH D-glukosa L-glukosa
Stereoisomers lainnya memiliki names yang unik, misalnya glukosa, manosa, galaktosa, dll. Jumlah stereoisomer adalah n2, dengan n adalah jumlah pusat asimetrik. Aldosa dengan 6-C memiliki 4 pusat asimetrik, oleh karenanya memiliki 16 stereoisomer (8 gula berbentuk D dan 8 gula berbentuk L).
Pembentukan hemiasetal & hemiketal
Aldehida dapat bereaksi dengan alkohol membentuk hemiasetal.
Keton dapat bereaksi dengan alkohol membentuk hemiketal.
C
R
R'
O
keton
aldehida
C
H
R
O
hemiasetal
O C
H
R
OHR'
alkohol
R' OH
hemiketal
O C
R
R'
OH" R
+
+ " R OH
alkohol
Pentosa dan heksosa dapat membentuk struktur siklik melalui reaksi gugus keton atau aldehida dengan gugus OH dari atom C asimetrik terjauh.Glukosa membentuk hemiasetal intra-molekular sebagai hasil reaksi aldehida dari C1 & OH dari atom C5, dinamakan cincin piranosa( segi 6). Penampilan dalam bentuk gula siklik disebut proyeksi Haworth
Perubahan FisherKe Hawort, gugusKiri di Fisher ditulisDi atas cincin dansebaliknya
H
OH
H
OHH
OH
CH2OH
H
H23
4
5
6
1
HC+
OH
C HHO
C OHH
C
O-H
H
CH OH
2
3
6
4
5
C
2
1
D - Glukosa
O- H+
Struktur FicherStruktur siklohemiasetal
H
CH O
OH
C HHO
C OHH
C
O
H
H
CH OH
2
3
6
4
5
C
2
1
α – D - Glukosa
O-H+
O-+
H O
OH
H
OHH
OH
CH2OH
H
OH
H H O
OH
H
OHH
OH
CH2OH
H
H
OH
-D- glukosa -D- glukosa
23
4
5
6
1 1
6
5
4
3 2
HC+
OH
C HHO
C OHH
C
O-H
H
CH OH
2
3
6
4
5
C
2
1
D - Glukosa
O- H+
Struktur Ficher Struktur siklohemiasetal
H
CH O
OH
C HHO
C OHH
C
O
H
H
CH OH
2
3
6
4
5
C
2
1
α – D - Glukosa
H
CH
OH
C HHO
C OHH
C
O
H
H
CH OH
2
3
6
4
5
C
2
1
β – D - Glukosa
O
H
CH O
OH
C HHO
C OHHO
C
H
H
CH OH
2
3
6
4
5
C
2
1
O
α. D galaktosaH OO
H
H
OHH
OH
CH2OH
H
OH
H
23
4
5
6
1
Monosakarida dapat membentuk Cincin piranosa, melalui reaksi antara gugus
aldehid atom C1 dengan OH dari C5. Cincin furanosa, melalui reaksi antara gugus keto
atom C2 dengan OH dari C5.
Pembentukan cincin siklik glukosa menghasilkan pusat asimetrik baru pada atom C1. Kedua stereoisomer disebut anomer, & .
Proyeksi Haworth menunjukkan bentuk cincin dari gula dengan perbedaan pada posisi OH di C1 anomerik :
(OH di bawah struktur cincin) (OH di atas struktur cincin).
H O
OH
H
OHH
OH
C H2OH
H
-D-glukosa
OH
H H O
OH
H
OHH
OH
C H2OH
H
H
OH
-D-glukosa
23
4
5
6
1 1
6
5
4
3 2
Karena sifat ikatan karbon yang berbentuk tetrahedral, gula piranosa membentuk konfigurasi “kursi" atau “perahu", tergantung dari gulanya.
Penggambaran konfigurasi kursi dari glukopiranosa di atas lebih tepat dibandingkan dengan proyeksi Haworth.
O
H
HO
H
HO
H
OHOHH
H
OH
O
H
HO
H
HO
H
HOHH
OH
OH
-D-glukopiranosa -D-glukopiranosa
1
6
5
4
32
Turunan gula
Gula alkohol – tidak memiliki gugus aldehida atau ketone; misalnya ribitol.
Gula asam –gugus aldehida pada atom C1, atau OH pada atom C6, dioksidasi membentuk asam karboksilat; misalnya asam glukonat, asam glukuronat.
CH2OH
C
C
C
CH2OH
H OH
H OH
H OH
D-ribitol
COOH
C
C
C
C
H OH
HO H
H OH
Asam D-glukonat Asam D-glukuronat
CH2OH
OHH
CH O
C
C
C
C
H OH
HO H
H OH
COOH
OHH
Oksidasi gula aldehida
C
C OHH
C HHO
C OHH
C OHH
CH2OH
D-glucose
OH
Oksidator
Asam D-glukonat
CO O H
C
C
C
C
H OH
HO H
H OH
CH2O H
OHH
Gula yang dapat dioksidasi adalah senyawa pereduksi. Gula yang demikian disebut sebagai gula pereduksi.
Senyawa yang sering digunakan sebagai pengoksidasi adalah ion Cu+2,terdapat dalam reagen fehling , campuran fehling A (larutan CuSO4) dan fehling B Larutan KNa – tartrat. yang berwarna biru cerah, yang akan tereduksi menjadi ion Cu+, yang berwarna merah kusam. Hal ini menjadi dasar bagi pengujian fehling/Benedict yang digunakan untuk menentukan keberadaan glukosa dalam urin, suatu pengujian bagi diagnosa diabetes.
Oksidasi gula aldehida
ISOMERI OPTIS Cahaya bergetar ke segala arah, jika cahaya tsb
dilewatkan pada kaca nikol hanya akan memiliki satu arah dinamakan cahaya yang terpolarisasi/terkukup. Kaca nikol disebut polarisator. Jika suatu zat dilewati cahaya terpolarisasi arah bidangnya diputar zat tersebut dinamai zat optis aktif. Zat bersifat optis aktif minimal memiliki 1 atom C khiral/asimetris, ke 4 tangannya mengikat gugus yang berbeda.
MUTAROTASI
Glukosa yang baru dilarutkan jika diukur sudut putarnya terhadap bidang polarisasi cahaya : 112o tetapi setelah didiamkam beberapa lama sudut putarnya menjadi 52,6 o . Peristiwa perubahan besarnya sudut ini disebut mutarotasi.
Oksidasi gula aldehida
Glukosa + Cu++
Gluconic acid + Cu2O (Cu2O is insol ppt)
Glukosa + O2
Asam glukonat + H2O2 (H2O2 nya diukur)
Glukosa + ATP
Glukosa-6-P + ADP (G-6-Pnya diukur)
panas & alk . pH
glukosa oksidase
heksokinase
Sifat2 mono sakaridaglukosa fruktosa galaktosa
Putar kanan Putar kiri Putar kanan
Mereduksi fehling
Mereduksi fehling Mereduksi fehling
Mengalami fermentasi
mengalami fermentasi
Tidak mengalami fermentasi
Mengalami mutarotasi
Gula amino - gugus amino menggantikan gugus hidroksil. Sebagai contoh glukosamina. Gugus amino dapat mengalami asetilasi, seperti pada N-asetilglukosamina.
Turunan gula
H O
OH
H
OH
H
NH 2H
OH
CH2OH
H
-D-glukosamina
H O
OH
H
OH
H
NH
OH
CH2OH
H
-D-N-asetilglukosamina
C CH 3
O
H
Disakarida
Gugus hidroksil anomerik dan gugus hidroksil gula atau senyawa yang lain dapat membentuk ikatan yang disebut ikatan glikosida dengan membebaskan air :
R-OH + HO-R' R-O-R' + H2OMisalnya pada maltosa gabungan 2 molekul glukosa gugus OH no. 4 dengan OH no. 1 dari glukosa yang berbeda membentuk ikatan glukosida.
O
H
HO
H
HO
H
OHOHH
H
OH
-D-glukopiranosa
O
H
HO
H
HO
H
OCH3
OHHH
OH
Metil--D-glukopiranosa
CH3- OH+
metanol
H2O
Struktur disakarida
Cellobiose, a product of cellulose breakdown, is the otherwise equivalent anomer (O on C1 points up). The (1 4) glycosidic linkage is represented as a zig-zag, but one glucose is actually flipped over relative to the other.
H O
O H
H
O HH
O H
CH 2O H
HO H
O H
H
O HH
O H
CH 2O H
H
O
HH
1
23
5
4
6
1
23
4
5
6
m altose
H O
O H
H
O HH
O H
CH 2O H
HO O H
H
H
O HH
O H
CH 2O H
H
H
H
O1
23
4
5
6
1
23
4
5
6
cellobiose
DisakaridaMaltosa, pemecahan produk pati (amylose), sebagai disakarida dengan ikatan (1 4) glucosida ikatan antara C1 - C4 OH dari 2 molekul glukosa.
Sifat2 DisakaridaMaltose
( gula pati)Sukrosa
(gula tebu)Laktosa
(gula susu)Putar kanan Putar kanan Putar kanan
Mereduksi fehling Tidak mereduksi fehling
Mereduksi fehling
Mengalami fermentasi
Tidak mengalami fermentasi
Tidak mengalami fermentasi
Mengalami inversi
Sukrosa(putar kanan)
+ H2O Glukosa(putar kanan)
+ Fruktosa(putar kiri yg lebih kuat
Campuran berputar kekiriPerubahan arah disebut inversi
Other disaccharides include: Sucrose, common table sugar, has a glycosidic bond linking
the anomeric hydroxyls of glucose & fructose.
Because the configuration at the anomeric C of glucose is (O points down from ring), the linkage is (12).
The full name of sucrose is -D-glucopyranosyl-(12)--D-fructopyranose.)
Lactose, milk sugar, is composed of galactose & glucose, with (14) linkage from the anomeric OH of galactose. Its full name is -D-galactopyranosyl-(1 4)--D-glucopyranose
Polysaccharides
Plants store glucose as amylose or amylopectin, glucose polymers collectively called starch. Glucose storage in polymeric form minimizes osmotic effects.Amylose is a glucose polymer with (14) linkages. It adopts a helical conformation. The end of the polysaccharide with an anomeric C1 not involved in a glycosidic bond is called the reducing end.
H O
OHH
OHH
OH
CH 2 OH
HO H
H
OHH
OH
CH 2 OH
H
O
HH H O
OH
OHH
OH
CH 2 OH
HH H O
H
OHH
OH
CH 2 OH
H
OH
HH O
OH
OHH
OH
CH 2 OH
H
O
H1
6
5
4
3
1
2
a m y lo s e
Amylopectin is a glucose polymer with mainly (14) linkages, but it also has branches formed by (16) linkages. Branches are generally longer than shown above.The branches produce a compact structure & provide multiple chain ends at which enzymatic cleavage can occur.
H O
OHH
OHH
OH
CH2OH
HO H
H
OHH
OH
CH2OH
H
O
HH H O
OH
OHH
OH
CH2
HH H O
H
OHH
OH
CH2OH
H
OH
HH O
OH
OHH
OH
CH2OH
H
O
H
O
1 4
6
H O
H
OHH
OH
CH2OH
HH H O
H
OHH
OH
CH2OH
HH
O1
OH
3
4
5
2
amylopectin
Glycogen, the glucose storage polymer in animals, is similar in structure to amylopectin. But glycogen has more (16) branches. The highly branched structure permits rapid release of glucose from glycogen stores, e.g., in muscle during exercise. The ability to rapidly mobilize glucose is more essential to animals than to plants.
H O
OHH
OHH
OH
CH 2OH
HO H
H
OHH
OH
CH 2OH
H
O
HH H O
OH
OHH
OH
CH 2
HH H O
H
OHH
OH
CH 2OH
H
OH
HH O
OH
OHH
OH
CH 2OH
H
O
H
O
1 4
6
H O
H
OHH
OH
CH 2OH
HH H O
H
OHH
OH
CH 2OH
HH
O1
OH
3
4
5
2
glycogen
Cellulose, a major constituent of plant cell walls, consists of long linear chains of glucose with (14) linkages.Every other glucose is flipped over, due to the linkages. This promotes intra-chain and inter-chain H-bonds and
c e l lu lo s e
H O
OHH
OHH
OH
CH 2 OH
HO
H
OHH
OH
CH 2 OH
HO
H H O
O H
OHH
OH
CH 2 OH
HH O
H
OHH
OH
CH 2 OH
H
H
OHH O
O H
OHH
OH
CH 2 OH
HO
H H H H
1
6
5
4
3
1
2
van der Waals interactions, that cause cellulose chains to be straight & rigid, and pack with a crystalline arrangement in thick bundles called microfibrils. Botany online website
Schematic of arrangement of cellulose chains in a microfibril.
Multisubunit Cellulose Synthase complexes in the plasma membrane spin out from the cell surface microfibrils consisting of 36 parallel, interacting cellulose chains. These microfibrils are very strong. The role of cellulose is to impart strength and rigidity to plant cell walls, which can withstand high hydrostatic pressure gradients. Osmotic swelling is prevented.Explore and compare structures of amylose & cellulose using Chime.
c e l lu lo s e
H O
OHH
OHH
OH
CH 2 OH
HO
H
OHH
OH
CH 2 OH
HO
H H O
O H
OHH
OH
CH 2 OH
HH O
H
OHH
OH
CH 2 OH
H
H
OHH O
O H
OHH
OH
CH 2 OH
HO
H H H H
1
6
5
4
3
1
2
Glycosaminoglycans (mucopolysaccharides) are polymers of repeating disaccharides. Within the disaccharides, the sugars tend to be modified, with acidic groups, amino groups, sulfated hydroxyl and amino groups, etc.Glycosaminoglycans tend to be negatively charged, because of the prevalence of acidic groups.
H O
H
H
O HH
O H
COO
H
H O
O H H
H
NH COCH 3H
CH 2O H
H
OO
D -g lucuronate
O
1
23
4
5
61
23
4
5
6
N -acetyl-D -g lucosam inehyaluronate
Hyaluronate is a glycosaminoglycan with a repeating disaccharide consisting of 2 glucose derivatives, glucuronate (glucuronic acid) & N-acetyl-glucosamine.
The glycosidic linkages are (13) & (14).
H O
H
H
O HH
O H
COO
H
H O
O H H
H
NH COCH 3H
CH 2O H
H
OO
D -glucuronate
O
1
23
4
5
61
23
4
5
6
N -acetyl-D -g lucosam inehyaluronate
Proteoglycans are glycosaminoglycans that are covalently linked to specific core proteins.
Some proteoglycans of the extracellular matrix in turn link non-covalently to hyaluronate via protein domains called link modules.
H O
H
H
O HH
O H
COO
H
H O
O H H
H
NH COCH 3H
CH 2O H
H
OO
D -glucuronate
O
1
23
4
5
61
23
4
5
6
N -acetyl-D -g lucosam inehyaluronate
For example, in cartilage multiple copies of the aggrecan proteoglycan bind to an extended hyaluronate backbone to form a large complex. Versican, another proteoglycan that binds to hyaluronate, is in the extracellular matrix of loose connective tissues. See web sites on aggrecan and aggrecan plus versican.
H O
H
H
O HH
O H
COO
H
H O
O H H
H
NH COCH 3H
CH 2O H
H
OO
D -glucuronate
O
1
23
4
5
61
23
4
5
6
N -acetyl-D -g lucosam inehyaluronate
Heparan sulfate is initially synthesized on a membrane-embedded core protein as a polymer of alternating N-acetylglucosamine and glucuronate residues. Later, in segments of the polymer, glucuronate residues may be converted to the sulfated sugar iduronic acid, while N-acetylglucosamine residues may be deacetylated and/or sulfated.
H O
H
OSO3H
OH
H
COOO H
H
NHSO3H
OH
CH2OSO3
H
H
H
O
O
heparin or heparan sulfate - examples of residues
iduronate-2-sulfate N-sulfo-glucosamine-6-sulfate
Heparin, a soluble glycosaminoglycan found in granules of mast cells, has a structure similar to that of heparan sulfates, but is more highly sulfated. When released into the blood, it inhibits clot formation by interacting with the protein antithrombin.
Heparin has an extended helical conformation.
heparin: (IDS-SGN)5
PDB 1RID
C O N SCharge repulsion by the many negatively charged groups may contribute to this conformation.
Heparin shown has 10 residues, alternating IDS (iduronate-2-sulfate) & SGN (N-sulfo-glucosamine-6-sulfate).
Some cell surface heparan sulfate glycosaminoglycans remain covalently linked to core proteins embedded in the plasma membrane. Proteins involved in signaling & adhesion at the cell surface recognize and bind segments of heparan sulfate chains having particular patterns of sulfation.
heparan sulfate glycosaminoglycan
cytosol
core protein
transmembrane -helix
O-linked oligosaccharide chains of glycoproteins vary in complexity. They link to a protein via a glycosidic bond between a sugar residue & a serine or threonine OH. O-linked oligosaccharides have roles in recognition, interaction, and enzyme regulation.
H O
OH
O
H
HNH
OH
CH2OH
H
C CH3
O
-D-N-acetylglucosamine
CH2 CH
C
NH
O
H
serine residue
Oligosaccharides that are covalently attached to proteins or to membrane lipids may be linear or branched chains.
H O
OH
O
H
HNH
OH
CH2OH
H
C CH3
O
-D-N-acetylglucosamine
CH2 CH
C
NH
O
H
serine residue
N-acetylglucosamine (GlcNAc) is a common O-linked glycosylation of protein serine or threonine residues. Many cellular proteins, including enzymes & transcription factors, are regulated by reversible GlcNAc attachment. Often attachment of GlcNAc to a protein OH alternates with phosphorylation, with these 2 modifications having opposite regulatory effects (stimulation or inhibition).
N-linked oligosaccharides of glycoproteins tend to be complex and branched. First N-acetylglucosamine is linked to a protein via the side-chain N of an asparagine residue in a particular 3-amino acid sequence.
H O
OH
HN
H
H
HNH
OH
CH2OH
H
C CH3
O
C CH2 CH
O HN
C
HN
O
HC
C
HN
HC
R
O
C
R
O
Asn
X
Ser or ThrN-acetylglucosamine
Initial sugar in N-linked glycoprotein oligosaccharide
Additional monosaccharides are added, and the N-linked oligosaccharide chain is modified by removal and addition of residues, to yield a characteristic branched structure.
NAN
Gal
NAG
Man
NAG
Gal
NAN
Man
Man
NAG
Gal
NAN
NAG
NAG
Asn
Fuc
N-linked oligosaccharide
Key:NAN = N-acetylneuraminateGal = galactoseNAG = N-acetylglucosamineMan = mannoseFuc = fucose
Many proteins secreted by cells have attached N-linked oligosaccharide chains.
Genetic diseases have been attributed to deficiency of particular enzymes involved in synthesizing or modifying oligosaccharide chains of these glycoproteins.
Such diseases, and gene knockout studies in mice, have been used to define pathways of modification of oligosaccharide chains of glycoproteins and glycolipids.
Carbohydrate chains of plasma membrane glycoproteins and glycolipids usually face the outside of the cell.
They have roles in cell-cell interaction and signaling, and in forming a protective layer on the surface of some cells.
Lectins are glycoproteins that recognize and bind to specific oligosaccharides. A few examples: Concanavalin A and wheat germ agglutinin
are plant lectins that have been useful research tools.
Mannan-binding lectin (MBL) is a glycoprotein found in blood plasma.
It associates with cell surface carbohydrates of disease-causing microorganisms, promoting phagocytosis of these organisms as part of the immune response.
A cleavage site just outside the transmembrane -helix provides a mechanism for regulated release of some lectins from the cell surface. A cytosolic domain participates in regulated interaction with the actin cytoskeleton.
transmembrane -helix
lectin domain selectin
cytoskeleton binding domain
cytosol
outside
Selectins are integral proteins of mammalian cell plasma membranes with roles in cell-cell recognition & binding. A lectin-like domain is at the end of an extracellular segment that extends out from the cell surface.