Fikosianin Dea Widyaningtyas 13.70.0160 E5 UNIKA SOEGIJAPRANATA

7/24/2019 Fikosianin Dea Widyaningtyas 13.70.0160 E5 UNIKA SOEGIJAPRANATA

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1. MATERI METODE

1.1. Materi

1.1.1. Alat

Ala# !an" i"una,an ala* +ra,#i,u* ini aalahsentrifuge1 +en"au,2stirrer1 o3en1 an

plate stirrer&

1.1.2. Baha

4ahan !an" i"una,an ala* +ra,#i,u* ini aalah 5io*asa S+irulina 5asah1 a6uaes1

an e,s#rin&

1.2. Met!"e

4io*assa S+irulina ,erin" i*asu,,an ala* erlen*en!er&

Disen#ri7u"asi -((( r+* sela*a $( *eni# lalu su+erna#an i+inah ,e "elas u,ur&

Diau, *en""una,an s#irrer sela*a ,uran" le5ih 0 8a*&

S+irulina ilaru#,an en"an a6uaes 9+er5anin"an $:$(

Se5a"ian su+erna#an +aa "elas u,ur iencer,an hin""a $(;0

,e*uian iu,ur ,aar 7i,osianinn!a en"an

s+e,#ro7o#o*e#er 9)$-n* an )-0 n*&


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Sisa su+erna#an +aa "elas u,ur i#a*5ah,an es,#rin en"an

+er5anin"an su+erna#an:es,#rin < =:> 9,elo*+o, E$1 E01 an

E% an $:$ 9,elo*+o, E? an E-&

Se#elah #erca*+ur ra#a lalu i#uan",an ,e ala* @aah !an"

a+a# i"una,an se5a"ai alas un#u, +roses +en"erin"an&

Dio3en +aa suhu ?- hin""a ,erin" ,uran" le5ih ,aar air

se,i#ar 'B 9cu,u+ ia*5il en"an s+a#ula an iliha# ,erin" a#au

*asih "e*+al&


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2. #ASIL PEN$AMATAN

Hasil +en"a*a#an 7i,osianin a+a# iliha# +aa Ta5el $&

Ta5el $& Hasil Pen"a*#an .i,osianin

Kel!%&!

'

Berat Bi!%a((a

Keri)

*)+

,-%lah a-a"e(

/a) "ita%0aha'a

*%l+

T!tal iltrat

/a) "i&er!leh

*%l+

OD

13

OD

32

KF

*%)4%l+ *

E$ = =( -) (1(--$

(1($)?

(1==)

E$ = =( -) (1(-'-

(1($)?

(1>%$

E% = =( -) (1()?'

(1($->

$1('(

E? = =( -) (1()$%

(1($??

$1(0(

E- = =( -) (1()0?

(1($')

$1($0

Ke#eran"an :WarnaC < 5iru

*uaCC < 5iru#uaCCC < 5irusan"a# #ua

4erasar,an #a5el hasil +en"a*a#an ia#as *enun8u,an 5era# 5io*assa ,erin" !an"

i"una,an sa*a +aa #ia+ ,elo*+o,1 5e"i#u 8u"a 8u*lah a6uaes !an" i#a*5ah,an&

Na*un hasil 7i,osianin !an" ia+a#,an #ia+ ,elo*+o, 5er5ea;5ea& Paa ,elo*+o,

E$ *ena+a#,an ,aar 7i,osianin se5esar )10(0 *"2*l& Paa ,elo*+o, E0

*ena+a#,an ,aar 7i,osianin se5esar )1-$' *"2*l& Paa ,elo*+o, E% *ena+a#,an

,aar 7i,osianin se5esar '1?>% *"2*l& Paa ,elo*+o, E? *ena+a#,an ,aar 7i,osianin

se5esar '1$?( *"2*l& Paa ,elo*+o, E- *ena+a#,an ,aar 7i,osianin se5esar '1(=?

*"2*l& #er8ai +eru5ahan @arna ari se5elu* ,i#osan i,erin",an an se#elah

i,erin",an& Peru5ahan @arna i#u *eli+u#i 5iru #ua *en8ai 5iru *ua +aa ,elo*+o,

E$ an E01 #ia, aan!a +eru5ahan @arna !ai#u #e#a+ 5iru *ua +aa ,elo*+o, E% an

E?1 ser#a +eru5ahan @arna ari 5iru san"a# #ua *en8ai 5iru #ua +aa ,elo*+o, E-&

5. PEMBA#ASAN

Kaar 7i,osianin iu,ur en"an ru*us:

Konsen#rasi .i,osianin2K. 9*"2*l +aa ,elo*+o, E$1 E0 an E%1 ser#a

+er5anin"an $ : $ un#u, ,elo*+o, E? an E-& Tu8uan ari +ena*5ahan e,s#rin ini

aalah un#u, *e*+erce+a# +en"erin"an an *ence"ah ,erusa,an a,i5a# +anas1

*ela+isi ,o*+onen flavor1 *enin",a#,an #o#al +aa#an ser#a *e*+er5esar 3olu*e

9Mur#ala1 $>>>& De,s#rin aalah +olisa,aria !an" ihasil,an ari +roses hirolisa +a#i

!an" ia#ur oleh eni* #er#en#u a#au hirolisis oleh asa*& De,s#rin ini 5er@arna 5er,isaran#ara +u#ih hin""a ,unin"& Si7a# e,s#rin ini *uah laru# ala* air1 le5ih ce+a#

#eri+ersi1 #ia, ,en#al1 ser#a le5ih s#a5il i5anin",an +a#i 9Re!nol1 $>=0&=&>& Menuru# Ri5ua# an Ku*alanin"sih 90((? 7un"si e,s#rin se5a"ai +e*5a@a

5ahan +an"an !an" a,#i7 se+er#i 5ahan flavoran +er@arna !an" *e*5u#uh,an si7a#

*uah laru# air an 5ahan +en"isi i,arena,an a+a# *enin",a#,an 5era# +rou, ala*

5en#u, 5u5u,& De,s#rin 8u"a *a*+u un#u, *elinun"i s#a5ili#as flavor +aa +roses

+en"erin"an en"an spray dryer !an" *en""una,an suhu +anas 9Su+ar#i1 0(((&


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S#ru,#ur *ole,ul e,s#rin 5er5en#u, s+iral1 sehin""a *ole,ul;*ole,ul flavor a,an

#er+eran",a+ i ala* s#ru,#ur ini& Pena*5ahan e,s#rin ini a+a# *en"uran"i

,o*+onen volatile!an" hilan" sela*a +en"olahan 5erlan"sun" 9Arie71 $>='&

$(&$$& Selan8u#n!a a+a5ila suah #erca*+ur se*ua laru#ann!a *a,a i#uan",an ,e

ala* @aah !an" a+a# i"una,an se5a"ai alas un#u, +roses +en"erin"an& Se#elah

ila,u,an +enuan"an ,e*uian i*asu,,an ,e ala* ovenen"an suhu ?- hin""a

,erin" en"an ,uran" le5ih ,aar air n!a *enca+ai se,i#ar 'B 9cu,u+ en"an

*en"a*5il s+a#ula an iliha# suah ,erin" a#au *asih "e*+al& Se#elah i,erin",an

*a,a a,an #erliha# a#au *e*5en#u, aonan ,erin" !an" "e*+al1 *a,a se#elah i#u

ila,u,an +en"hancuran 7i,osianin !an" #elah ,erin" ini en"an ala# +enu*5u, hin""a

5er5en#u, +o@er& Pen"erin"an en"an *en""una,an suhu ?- ini suah sesuai1

,arena *enuru# Des*orieuF Dacaen 90(() 5ah@a 8i,a *en""una,an suhu ia#as

)( a,an *en!e5a5,an #er8ain!a e"raasi 7i,osianin an #i*5uln!a rea,si maillard&

Pen"erin"an en"an *en""una,an sinar *a#ahari lan"sun" a+a# i"una,an na*un

a,an a+a# *eni*5ul,an aro*a !an" #ia, iin"in,an an 8u"a a,an *enin",a#,an

,on#a*inasi 5a,#eri& Paa +roses +en!i*+anan Spirulina a+a# ila,u,an ala*

,eaaan ,erin"1 i,arena,an ala* ,onisi ini Spirulina #ia, *uah *en"ala*i

7er*en#asi 9An",a Suhar#ono1 0(((&$0&$%& Paa hasil !an" ia+a#,an 5ah@a +aa #ia+ ,elo*+o, *en""una,an 5era#

5io*asa ,erin" se5an!a, = "ra* ,e*uian i#a*5ah a6uaes se5an!a, =( *l sehin""a

ia+a#,an #o#al 7il#ra#e !an" sa*a !ai#u -) *l& +aa +en"u,uran optical density 9OD)$-

an OD)-0 ia+a#,an hasil !an" 5er5ea ari #ia+ ,elo*+o,& Hal ini i,arena,an

*e#oe a5sor5ansi i+en"aruhi oleh ,onsen#rasi an ,e8ernihan ari laru#an & Se*a,in

+e,a# an ,eruh sua#u laru#an *a,a nilai a5sor5ansi !an" ia+a#,an a,an se*a,in

#in""i 9.oF1 $>>$& Sean",an nilai K. 9*"2*l i"una,an un#u, *en"hi#un"yield ari

7i,osianin& Yield !an" ia+a#,an +aa ,elo*+o, E$ se5esar )10(0 *"2*l1 ,elo*+o,

E0 se5esar )1-$' *"2*l1 ,elo*+o, E% se5esar '1?>% *"2*l1 ,elo*+o, E? se5esar

'1$?( *"2*l1 ser#a +aa ,elo*+o, E- *ena+a#,an yieldse5esar '1(=? *"2*l& #erliha#

5ah@a nilai ,onsen#rasi !an" #in""i a,an *e*5ua# nilaiyield!an" se*a,in 5esar +ula&$?&$-& Warna ari 7i,osianin !an" #er5en#u, ari #ia+ ,elo*+o, 5er5ea;5ea& Paa

,elo*+o, E$ an E0 *en"ala*i +eru5ahan @arna ari 5iru #ua *en8ai 5iru *ua&


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Paa ,elo*+o, E% an E? #ia, *en"ala*i +eru5ahan @arna1 sean",an ,elo*+o, E-

*en"ala*i +eru5ahan @arna ari 5iru san"a# #ua *en8ai 5iru #ua& Peru5ahan @arna ini

i,arena,an oleh e,s#rin !an" i#a*5ah,an en"an ,onsen#rasi !an" #in""i *a,a a,an

*e*5ua# 5u5u, 7i,osianin !an" *e*ili,i @arna !an" *e*uar a#au cenerun" #ua1

sehin""a @arna 7i,osianin !an" 5iru #ua *an8ai 5iru *ua 9Wi!ono1 0(('& De,s#rin

*e*ili,i @arna +u#ih sehin""a a,an a+a# *e*uar,an @arna 7i,osianin !an"

ia+a#,an& Paa ,elo*+o, E? an E- *en""una,an ,onsen#rasi e,s#rin !an" sa*a

na*un hasil @arnan!a 5er5ea1 hal ini i,arena,an ,e#ia,#eli#ian +aa saa#

*eni*5an" e,s#rin !an" a,an i"una,an&$)&

$'& Spirulina aalah or"anis*e !an" #er*asu, ala* ,elo*+o, al"a hi8au 5irua#au serin" ise5u# en"an blue green algae& Spirulina ini *eru+a,an

or"anis*e *ul#iseluler !an" #u5uhn!a 5er5en#u, 7ila*en# 5er@arna hi8au;

5iru 5er5en#u, siliner an #ia, 5erca5an"& U,uran ari or"anis*e ini

aalah $(( ,ali le5ih 5esar ari sel arah *anusia 9Rich*on1 $>==&

Diala* ,oloni !an" 5esar or"anis*e ini 5er@arna hi8au #ua& Warna ini

ise5a5,an ,arena aan!a ,loro7il ala* 8u*lah !an" #in""i& Secara ala*i

spirulina#u*5uh i +erairan anau !an" 5ersi7a# al,ali an suhu han"a# a#au

,ola* an",al i @ila!ah #ro+is 9Tie#e1 0((?& Menuru# Pra5u#has et al&

90($$ Spirulina platensis *eru+a,an c!ano5ac#eriu* +ro,ario#i,

5er7ila*en an en"an *uah i,ul#ur,an an i+anen& ;+h!cooc!anin

aalah *a!or 7i,o5ilin +aa Spirulina& Pi"*en !an" #era+a# oaa 7i,osianin

an ,loro7il !ai#u +i"*en 5iru& 4io*assa Spirulina +i"*en hilan" saa#

+roses s+e,#ro7o#o*e#ri&

$=&

$>& Menuru# Suha Ka3i*ani 90($$ s+irulina *en"anun" -(;'(B +ro#ein

an ,a!a a,an 3i#a*in1 #eru#a*a 3i#a*in 4$01 ;caro#ene 9+ro3i#a*in A1 an

3i#a*in E& S+irulina 8u"a *en"anun" 7i,osianin se5an!a, 'B ari 5era#

,erin" ser#a *en"anun" +olisa,aria !an" *e*ili,i si7a# an#io,sian&

Menuru# Tie#e 90((? Spirulina secara ala*i *e*ili,i ,anun"an

,oles#erol1 ,alori1 le*a, an soiu* !an" renah& Or"anis*e ini 8u"a

*en"anun" Se*5ilan 3i#a*in !an" oen#in" ari e*+a# 5elas *ineral !an"

#er,ai# en"an asa* a*ino& Kanun"an ini !an" *en!e5a5,an +roses


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asi*ilasi +aa #u5uh 5er8alan *en8ai le5ih ce+a#& Spirulinaini *e*+un!ai

*e*5ransel !an" #i+is an le*5u# sehin""a *uah icerna an ala*

+en"olahann!a #ia, *e*5u#uh,an +en"olahan !an" ,husus& Spirulina

*en"nun" +ro#ein se5esar )(;'(B ari 5era# ,erin"n!a1 ,a!a a,an

,anun"an li+i !an" 5er5en#u, asa* le*a, 8enuh !ai#u #eru#a*a gamma

linolenic acidse5a"ian 5esarn!a& Or"anis*e ini a+a# i*an7aa#,an se5a"ai

an#io,sian !an" 5isa i"una,an ala* o5a#;o5a#an 9Mon#eiro et al, 0($(&

0(&

0$& Spirulina *e*ili,i *e*5ran #ila,oi !an" iala*n!a #era+a# s#ru,#ur

"ranula 5eru+a 7i,o5iliso* !an" #eriri ari 7i,o5ili+ro#ein& .i,o5ili+ro#ein

ini *e*+un!ai 7un"si un#u, *en!era+ caha!a an 8u"a *elinun"i +i"*en7o#osin#esis lainn!a ari o,siasi +aa caha!a en"an in#ensi#as !an" #in""i&

aha!a !an" #elah isera+ oleh 7i,osianin a,an i#rans7er ,e allo7i,osianin

!an" ,e*uian a,an i#erus,an *enu8u +usa# rea,si1 !ai#u ,loro7il i

*e*5rane #ila,oi& Kloro7il *eru+a,an +i"*en 7o#osin#esis Spirulina!an"

#erle#a, +aa *e*5ran #ila,oi i ala* ,ro*o+las 9Dihar*i1 0(($&

Pi"*en !an" #era+a# ala* Spirulina #er5a"i *en8ai #i"a ,elas1 !ai#u

,loro7il se5esar $1'B ari 5era# sel1 ,ara#enoi an Fan#o7il se5esar (1-B5era# sel1 an 7i,o5ili+ro#ein !an" #eriri ari 0(B +ro#ein seluler an secara

,uan#i#a#i7 *eru+a,an +i"*en !an" +alin" o*inan +aa Spirulina

9Rich*on1 $>==& Sa*a haln!a en"an +ern!a#aan Marre et al. 90($%

s+irulina *en"anun" 5an!a, +i"*en 7o#osin#esis en"an ,onsen#rasi 0(B

ari 5era# ,erin"1 se+er#i ,loro7il1 5e#a;,aro#en1 7i,osianin an Fan#ho+h!lls&

.i,osianin *eru+a,an su*5er +i"*en @arna 5iru !an" +en#in" un#u,

+e@arna *a,anan1 !an" #erseia i Spirulina+aa ,onsen#rasi an#ara %(;00("2,"&

00&

0%& Pi"*en 7i,osianin *enun8u,an @arna 5iru #ua an 5isa *e*ancar,an @arna

*erah #ua& Pi"*en ini *asu, iala* ,elo*+o, 5ili+ro#ein i*ana *e*ili,i

,e*a*+uan se5a"ai +en"ha*5a# ala* #er5en#u,n!a ,oloni ,an,er& Kelo*+o, +i"*en

!an" 5isa i#e*u,an +aa al"a *erah1 al"a hi8au;5iru1 an al"a cr!#o*ona ialah

7i,o5ili+ro#ein a#au 5ili+ro#ein 9J arra J hEocha1 $>')& Menuru# Shih et al., 90((>

an Ro*a! et al,.90((% 7i,osianin *e*+un!ai ,e"unaan !ai#u se5a"ai +e@arna ala*i


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an isa*+in" i#u *e*ili,i ,e"unaan se5a"ai +en!e*5uhan1 i*ana se5a"ai

an#io,sian an a+a# +ula *en8ai an#i raan"& Pe*uaran @arna +aa laru#an

7i,osianin se5esar %(B sesuah isi*+an sela*a - hari an 5enin" sesuah $- hari i

suhu %(& +i"*en 7i,osianin ini 5an!a, #er,anun" +aa al"a hi8au 5iru 9Mishara et

al,. 0((=& Pan8an" "elo*5an" se,i#ar -?) n* *eru+a,an nilai a5sor5ansi caha!a

*a,si*u* ari 7i,osianin 9J arra J hEocha1 $>')& Tera+a# ran#ai tetraphyrroles

#er5u,a +aa s#ru,#ur 7i,osianin i*ana *e*+un!ai ,e*a*+uan ala* *enan",a+

rai,al o,si"en 9Ro*a! et al.,$>>=&0?&

0-& Menuru# ahroo8ian et al& 90($% al"a 5iru hi8au !ai#u Spirulina platensis ini

suah la*a *en8ai su*5er *a,anan 5a"i *anusia an he@an ,arena*e*ili,i nu#risi !an" 5ai, an #in""i ,aro#enoi& S+irulina *eru+a,an

su*5er ala*i ari ,aro#enoi !an" e7e,#i7 un#u, +e@arnaan +aa ,unin"

#elur& Di +asaran1 *i,roal"a ,aro#enoi ini 5ersain" en"an +e@arna sin#e#i,

un#u, *e*5en#u, +i"*en& Menuru# Saranra8 Si3asa,#hi 90($? 7i,osianin

aalah +i"*en 5iru !an" laru# air !an" 5erasal ari #in#a spirulina !an"

i#e*u,an +aa al"a hi8au 5iru& .i,osianin *eru+a,an an#io,sian laru# air&

Spirulinaini *en"aun" c;+h!coc!anin !an" *eru+a,an +ro#ein s#a5il !an"

a+a# *en8ai se5a"ai an#i5oi&

2. KESIMPULAN

27.

Spirulina aalah or"anis*e !an" #er*asu, ala* ,elo*+o, al"a hi8au 5iru a#au

ise5u# en"an blue green algae& U,u#an ari Spirulinaaalah $(( ,ali le5ih 5esar ari sel arah *anusia& Spirulina #u*5uh i +erairan anau !an" 5ersi7a# al,ali an suhu han"a# a#au

,ola* an",al i @ila!ah #ro+is& Kanun"an +ro#ein ari Spirulinacu,u+ #in""i Spirulinasecara ala*i *e*ili,i ,anun"an ,oles#erol1 ,alori1 le*a, an soiu*

!an" renah& Spirulina *e*ili,i *e*5ran #ila,oi !an" iala*n!a #era+a# s#ru,#ur "ranula

5eru+a 7i,o5iliso* !an" #eriri ari 7i,o5ili+ro#ein Pi"*en 7i,osianin *enun8u,an @arna 5iru #ua an 5isa *e*ancar,an @arna

*erah #ua&


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Pi"*en 7i,osianin *asu, iala* ,elo*+o, 5ili+ro#ein i*ana *e*ili,i

,e*a*+uan se5a"ai +en"ha*5a# ala* #er5en#u,n!a ,oloni ,an,er .i,osianin *e*+un!ai ,e"unaan !ai#u se5a"ai +e@arna ala*i1 +en!e*5uhan1

i*ana se5a"ai an#io,sian an a+a# +ula *en8ai an#i raan"& Pi"*en 7i,osianin ini 5an!a, #er,anun" +aa al"a hi8au 5iru& Tera+a# ran#ai tetraphyrroles #er5u,a +aa s#ru,#ur 7i,osianin i*ana

*e*+un!ai ,e*a*+uan ala* *enan",a+ rai,al o,si"en& Pena*5ahan air +aa isolasi +i"*en 7i,osianin un#u, +roses e,s#ra,si ,arena

+i"*en 7i,osianin ari Spirulina a+a# laru# +aa +elaru# +olar& Proses +en"au,an en"an stirer ila,u,an en"an #u8uan un#u,

*en"ho*o"en,an Spirulina en"an a6uaes an a+a# *e*a,si*al,an e,s#ra,si

+i"*en 7i,osianin& .un"si sen#ri7u"asi +aa isolasi +i"*en 7i,osianin un#u, *en"ena+,an e5ris sel

an *en"a*5il +i"*en 7i,osianin !an" laru# ala* a6uaes Tu8uan ila,u,ann!a +en"u,uran a5sor5ansi +aa isolasi 7i,osianin un#u,

*en"e#ahui ,elaru#an ari 7i,osianin De,s#rin aalah +olisa,aria !an" ihasil,an ari +roses hirolisa +a#i !an" ia#ur

oleh eni* #er#en#u a#au hirolisis oleh asa*& Si7a# e,s#rin *uah laru# ala* air1 le5ih ce+a# #eri+ersi1 #ia, ,en#al1 ser#a

le5ih s#a5il i5anin",an +a#i&

Tu8uan +ena*5ahan e,s#rin un#u, *e*+erce+a# +en"erin"an an *ence"ah,erusa,an a,i5a# +anas1 *ela+isi ,o*+onen flavor1 *enin",a#,an #o#al +aa#an

ser#a *e*+er5esar 3olu*e Paa +roses +en!i*+anan Spirulina a+a# ila,u,an ala* ,eaaan ,erin"1

i,arena,an ala* ,onisi ini Spirulina#ia, *uah *en"ala*i 7er*en#asi De,s#rin *e*ili,i @arna +u#ih sehin""a a,an a+a# *e*uar,an @arna

7i,osianin !an" ia+a#,an&

28.

29.

30.

31.

32.

33.

34.

35.

36.

37.

38.

39.

40.

41.


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42.

43.

44.

?-& Se*aran"1 - No3e*5er 0($-

?)& Pra,#i,an1 Asis#en Dosen1; Deanna Sun#oro; .er!an#o /u@ono

?'&?=&?>&-(&-$&52.Dea Wi!anin"#!as

35. DAFTAR PUSTAKA

-?&

--& Ach*ai SS1 /a!ai1 Tri;Pan8i&90((0& Prou,si +i"*en oleh Spirulinaplatensis!an" i#u*5uh,an +aa *eia li*5ah la#e,s +e,a#&Ha!a#i& >9%:=(;=?&

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Fikosianin Dea Widyaningtyas 13.70.0160 E5 UNIKA SOEGIJAPRANATA

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