dr.Iva Puspitasari,Sp.MK

23
dr.Iva Puspitasari,Sp.MK

description

dr.Iva Puspitasari,Sp.MK. Spesimen unt Pemeriksaan Mikrobiologi 􀃆 tatacara 􀀹pengambilan, 􀀹penampungan, 􀀹penyimpanan, 􀀹pemberian label dan 􀀹cara pengiriman spesimen. Tujuan: 􀀹tidak dicemari oleh bakteri lain, 􀀹bakteri di dalam spesimen tidak mati. - PowerPoint PPT Presentation

Transcript of dr.Iva Puspitasari,Sp.MK

Page 1: dr.Iva Puspitasari,Sp.MK

drIva PuspitasariSpMK

Spesimen unt Pemeriksaan Mikrobiologi 1048774 tatacara1048774pengambilan1048774penampungan1048774penyimpanan1048774pemberian label dan1048774cara pengiriman spesimen Tujuan1048774tidak dicemari oleh bakteri lain1048774bakteri di dalam spesimen tidak mati

Memuat Identitas penderita yang meliputi1048774 Nama1048774 Umur1048774 Jenis Kelamin1048774 Jenis permintaan pemeriksaanbiakan apa1048774 Jam dan Tanggal pengambilan spesimen Jika sampel gt 1 per Px

beri tanda lsquo1 dari 3rsquolsquo2 dari 3rsquo3 dari 3rsquo

INFORMASI UMUM Urin normal adalah steril Pencemaran uretra atau periuretra Urin kumpulan 24 jam Tidak bolehJENIS SPESIMEN URIN Urin kateter Urin porsi tengah ( (lsquoClean Catch Urinersquo) Urin Aspirasi Suprapubik

PEMILIHAN Kateter resiko memasukkan bakteri Jangan pakai Bed side Catheter bag PENGAMBILAN SPESIMEN II1 Bahan yang dibutuhkan Semprit isi 10 ml dan Jarum suntik nomor 21 Kapas alkohol

helliphellipUrine kateter

II2 Cara Pengambilan Sampel Jepit kateter (lt30 menit) Bersihkan dgn Alkohol pd tempat ambil urin Tusukkan jarum ambil urin tampung tutup

rapat CARA PEMBERIAN LABEL Pedoman Umum Cara Pemberian Label Cantumkan cara pengambilan urin misalnya Kateter

III PENGIRIMAN Segera priksa dalam 30 menit atau taruh dalam almari es dan paling lama 24 jam

URIN PORSI TENGAH(lsquoCLEAN CATCH URINErsquo)

I PEMILIHAN Dianjurkan urin pagi hari Buang 13 aliran urin pertama

PENGAMBILANII1 Bahan yang dibutuhkan

BotolTabung steril bertutup ulir Sabun medis Kasa Akuadesair suling

CARA PENGAMBILAN SPESIMENPenderita diberitahu (Lisantertulis) Instruksi pada Wanita1048774Duduk di toilet1048774Buka kakilutut ke samping selebar mungkin1048774Dgn sabun medis amp sponkainkapas cuci genital dgn gerak dari depan ke belakang1048774Bilas dengan spon basah depan ke belakang1048774Ulangi beberapa kali dgn spon basah baru1048774Pegang dengan jari dan taruh cawanbotol mulut

lebar di depan genital dan jangan menyentuhtepi botol

1048774Buang urin pertama keluar dan1048774Berikutnya ditampung1048774Tutup segera botol

Instruksi pada Laki Tarik kulit preputium (lsquoForeskinrsquo untuk yang

tidak khitan) dan bersihkan Glans penis Ikuti cara pencucian seperti pada wanita

Periksa bahwa tutup rapat dan tidak pecah dan

Jika tak segera diperiksa almari es

CARA PEMBERIAN LABEL Baca pedoman Umum Cara Pemberian Label Catat apakah penderita telah mendapat

antibiotk CATATAN pd anak pakai Pediatric bag

PEMILIHAN Cara ini terbebas dari1048774pencemar uretra dan1048774perineum Diutamakan untuk1048774anak atau1048774pemeriksaan anaerobik PENGAMBILAN SPESIMEN1048774Bahan yang Dibutuhkan1048774Desinfektan kulit1048774Anastesi lokal1048774Semprit isi 10 ml dan jarum nomor 221048774Botol steril bertutup ulir

CARA PENGAMBILAN SPESIMEN Desinfeksi kulit Antar Pusar (Umbilicus) sd penis Anastesi pada tempat tusukan

( Mid-line 2 CM di atas simpisis) Masukkan jarum ke k kemih yang sedang penuh Hisap tampung dalam botol dan tutup rapat

CARA PEMBERIAN LABEL Baca Pedoman Umum Cara Pemberian Label Beri catatan1048774Urin suprapubik1048774Apakah perlu pemeriksaan anaerobik1048774Catat waktu pengambilan (Jam tanggal)

PENGIRIMAN Segera periksa dalam 30 menit atau taruh dalam almari es dan paling lama 24

jam

CATATAN Pemeriksaan anaerobik hanya atas

permintaan Anak tempat tusukan 1-2 CM di atas simpisis

pubis Jml 5 ml

BILA JUMLAH KOLONI ML URIN

lt10000 DIANGGAP KONTAMINASI

10000-100000 SANGSI PERLU DIULANG 100000 SIGNIFICANT BACTERIURIA

(JELAS UTI)

Kriteria klasifikasi UTI berdasarkan sindroma klinik

Category Clinical laboratory

Acute Uncomplicated UTI in women

bullDysuria urgencyfrequencysuprapubic painbullNo urinary symptom in last 4 wk before current episodebullNo fever or flank pain

ge10 WBCmm3

ge 103 CFUml uropathogens in CCMS urine

Acute uncomplicated pyelonephritis

bullFever chillsbullFlank pain on examinationbullOther diagnosis exludedbullNo history or clinical evidence of uropathologic abnormalities

ge10 WBCmm3

ge 104 CFUml uropathogens in CCMS urine

Complicated UTI and UTI in men

bullAny combination of symptom listed abovebullOne or more factors assosiated with complicated UTI

ge10 WBCmm3

ge 105 CFUml uropathogens in CCMS urine

Asymptomatic UTI bullNo urinary symptoms plusmngt10 WBCmm3

ge 105 CFUml in two CCMS cultures gt24 hrs apart

Factors assosiated with complicated UTI include any UTIin male indwelling or intermitten urinary catheter gt100ml of postvoided residual urine obstructionuropathy urologic abnormalities azotemia amp renal transplantationCCMS Clean Catch Midstream urine

Pedoman umum interpretasi kultur urin

Result Spsific specimen typeassociated clinical condition if known

ge104 CFUml of single potential pathogen or for each of two potential pathogens

CCMS urinepyellonephritis acute cystitis asymptomatic bacteriuria or catheterized urines

complete

ge103 CFUml of single potential pathogen

CCMS urineasymptomatic males or catheterized urines or acute urethral syndrome

Complete

ge three organisme types with no predominating organism

CCMS urine or catheterized urines

None because of possible contamination ask for another specimen

Either two or three organism types with predominant growth of one organism type and lt104 CFUml of the other organism type(s)

CCMS urine Complete workup for predominating oganism(s) description for the other organism(S)

ge102 CFUml of any number of organism types (set up with a 0001-and 001-ml calibrated loop)

Suprapubic aspirates any other surgically obtained urines (including ileal conduits cystoscopy specimens)

Complete

CFU colony forming unit Predominant growth= 104 to ge105 CFUml A complete workup includes indentification of organism and appropriate susceptibility testing

Page 2: dr.Iva Puspitasari,Sp.MK

Spesimen unt Pemeriksaan Mikrobiologi 1048774 tatacara1048774pengambilan1048774penampungan1048774penyimpanan1048774pemberian label dan1048774cara pengiriman spesimen Tujuan1048774tidak dicemari oleh bakteri lain1048774bakteri di dalam spesimen tidak mati

Memuat Identitas penderita yang meliputi1048774 Nama1048774 Umur1048774 Jenis Kelamin1048774 Jenis permintaan pemeriksaanbiakan apa1048774 Jam dan Tanggal pengambilan spesimen Jika sampel gt 1 per Px

beri tanda lsquo1 dari 3rsquolsquo2 dari 3rsquo3 dari 3rsquo

INFORMASI UMUM Urin normal adalah steril Pencemaran uretra atau periuretra Urin kumpulan 24 jam Tidak bolehJENIS SPESIMEN URIN Urin kateter Urin porsi tengah ( (lsquoClean Catch Urinersquo) Urin Aspirasi Suprapubik

PEMILIHAN Kateter resiko memasukkan bakteri Jangan pakai Bed side Catheter bag PENGAMBILAN SPESIMEN II1 Bahan yang dibutuhkan Semprit isi 10 ml dan Jarum suntik nomor 21 Kapas alkohol

helliphellipUrine kateter

II2 Cara Pengambilan Sampel Jepit kateter (lt30 menit) Bersihkan dgn Alkohol pd tempat ambil urin Tusukkan jarum ambil urin tampung tutup

rapat CARA PEMBERIAN LABEL Pedoman Umum Cara Pemberian Label Cantumkan cara pengambilan urin misalnya Kateter

III PENGIRIMAN Segera priksa dalam 30 menit atau taruh dalam almari es dan paling lama 24 jam

URIN PORSI TENGAH(lsquoCLEAN CATCH URINErsquo)

I PEMILIHAN Dianjurkan urin pagi hari Buang 13 aliran urin pertama

PENGAMBILANII1 Bahan yang dibutuhkan

BotolTabung steril bertutup ulir Sabun medis Kasa Akuadesair suling

CARA PENGAMBILAN SPESIMENPenderita diberitahu (Lisantertulis) Instruksi pada Wanita1048774Duduk di toilet1048774Buka kakilutut ke samping selebar mungkin1048774Dgn sabun medis amp sponkainkapas cuci genital dgn gerak dari depan ke belakang1048774Bilas dengan spon basah depan ke belakang1048774Ulangi beberapa kali dgn spon basah baru1048774Pegang dengan jari dan taruh cawanbotol mulut

lebar di depan genital dan jangan menyentuhtepi botol

1048774Buang urin pertama keluar dan1048774Berikutnya ditampung1048774Tutup segera botol

Instruksi pada Laki Tarik kulit preputium (lsquoForeskinrsquo untuk yang

tidak khitan) dan bersihkan Glans penis Ikuti cara pencucian seperti pada wanita

Periksa bahwa tutup rapat dan tidak pecah dan

Jika tak segera diperiksa almari es

CARA PEMBERIAN LABEL Baca pedoman Umum Cara Pemberian Label Catat apakah penderita telah mendapat

antibiotk CATATAN pd anak pakai Pediatric bag

PEMILIHAN Cara ini terbebas dari1048774pencemar uretra dan1048774perineum Diutamakan untuk1048774anak atau1048774pemeriksaan anaerobik PENGAMBILAN SPESIMEN1048774Bahan yang Dibutuhkan1048774Desinfektan kulit1048774Anastesi lokal1048774Semprit isi 10 ml dan jarum nomor 221048774Botol steril bertutup ulir

CARA PENGAMBILAN SPESIMEN Desinfeksi kulit Antar Pusar (Umbilicus) sd penis Anastesi pada tempat tusukan

( Mid-line 2 CM di atas simpisis) Masukkan jarum ke k kemih yang sedang penuh Hisap tampung dalam botol dan tutup rapat

CARA PEMBERIAN LABEL Baca Pedoman Umum Cara Pemberian Label Beri catatan1048774Urin suprapubik1048774Apakah perlu pemeriksaan anaerobik1048774Catat waktu pengambilan (Jam tanggal)

PENGIRIMAN Segera periksa dalam 30 menit atau taruh dalam almari es dan paling lama 24

jam

CATATAN Pemeriksaan anaerobik hanya atas

permintaan Anak tempat tusukan 1-2 CM di atas simpisis

pubis Jml 5 ml

BILA JUMLAH KOLONI ML URIN

lt10000 DIANGGAP KONTAMINASI

10000-100000 SANGSI PERLU DIULANG 100000 SIGNIFICANT BACTERIURIA

(JELAS UTI)

Kriteria klasifikasi UTI berdasarkan sindroma klinik

Category Clinical laboratory

Acute Uncomplicated UTI in women

bullDysuria urgencyfrequencysuprapubic painbullNo urinary symptom in last 4 wk before current episodebullNo fever or flank pain

ge10 WBCmm3

ge 103 CFUml uropathogens in CCMS urine

Acute uncomplicated pyelonephritis

bullFever chillsbullFlank pain on examinationbullOther diagnosis exludedbullNo history or clinical evidence of uropathologic abnormalities

ge10 WBCmm3

ge 104 CFUml uropathogens in CCMS urine

Complicated UTI and UTI in men

bullAny combination of symptom listed abovebullOne or more factors assosiated with complicated UTI

ge10 WBCmm3

ge 105 CFUml uropathogens in CCMS urine

Asymptomatic UTI bullNo urinary symptoms plusmngt10 WBCmm3

ge 105 CFUml in two CCMS cultures gt24 hrs apart

Factors assosiated with complicated UTI include any UTIin male indwelling or intermitten urinary catheter gt100ml of postvoided residual urine obstructionuropathy urologic abnormalities azotemia amp renal transplantationCCMS Clean Catch Midstream urine

Pedoman umum interpretasi kultur urin

Result Spsific specimen typeassociated clinical condition if known

ge104 CFUml of single potential pathogen or for each of two potential pathogens

CCMS urinepyellonephritis acute cystitis asymptomatic bacteriuria or catheterized urines

complete

ge103 CFUml of single potential pathogen

CCMS urineasymptomatic males or catheterized urines or acute urethral syndrome

Complete

ge three organisme types with no predominating organism

CCMS urine or catheterized urines

None because of possible contamination ask for another specimen

Either two or three organism types with predominant growth of one organism type and lt104 CFUml of the other organism type(s)

CCMS urine Complete workup for predominating oganism(s) description for the other organism(S)

ge102 CFUml of any number of organism types (set up with a 0001-and 001-ml calibrated loop)

Suprapubic aspirates any other surgically obtained urines (including ileal conduits cystoscopy specimens)

Complete

CFU colony forming unit Predominant growth= 104 to ge105 CFUml A complete workup includes indentification of organism and appropriate susceptibility testing

Page 3: dr.Iva Puspitasari,Sp.MK

Memuat Identitas penderita yang meliputi1048774 Nama1048774 Umur1048774 Jenis Kelamin1048774 Jenis permintaan pemeriksaanbiakan apa1048774 Jam dan Tanggal pengambilan spesimen Jika sampel gt 1 per Px

beri tanda lsquo1 dari 3rsquolsquo2 dari 3rsquo3 dari 3rsquo

INFORMASI UMUM Urin normal adalah steril Pencemaran uretra atau periuretra Urin kumpulan 24 jam Tidak bolehJENIS SPESIMEN URIN Urin kateter Urin porsi tengah ( (lsquoClean Catch Urinersquo) Urin Aspirasi Suprapubik

PEMILIHAN Kateter resiko memasukkan bakteri Jangan pakai Bed side Catheter bag PENGAMBILAN SPESIMEN II1 Bahan yang dibutuhkan Semprit isi 10 ml dan Jarum suntik nomor 21 Kapas alkohol

helliphellipUrine kateter

II2 Cara Pengambilan Sampel Jepit kateter (lt30 menit) Bersihkan dgn Alkohol pd tempat ambil urin Tusukkan jarum ambil urin tampung tutup

rapat CARA PEMBERIAN LABEL Pedoman Umum Cara Pemberian Label Cantumkan cara pengambilan urin misalnya Kateter

III PENGIRIMAN Segera priksa dalam 30 menit atau taruh dalam almari es dan paling lama 24 jam

URIN PORSI TENGAH(lsquoCLEAN CATCH URINErsquo)

I PEMILIHAN Dianjurkan urin pagi hari Buang 13 aliran urin pertama

PENGAMBILANII1 Bahan yang dibutuhkan

BotolTabung steril bertutup ulir Sabun medis Kasa Akuadesair suling

CARA PENGAMBILAN SPESIMENPenderita diberitahu (Lisantertulis) Instruksi pada Wanita1048774Duduk di toilet1048774Buka kakilutut ke samping selebar mungkin1048774Dgn sabun medis amp sponkainkapas cuci genital dgn gerak dari depan ke belakang1048774Bilas dengan spon basah depan ke belakang1048774Ulangi beberapa kali dgn spon basah baru1048774Pegang dengan jari dan taruh cawanbotol mulut

lebar di depan genital dan jangan menyentuhtepi botol

1048774Buang urin pertama keluar dan1048774Berikutnya ditampung1048774Tutup segera botol

Instruksi pada Laki Tarik kulit preputium (lsquoForeskinrsquo untuk yang

tidak khitan) dan bersihkan Glans penis Ikuti cara pencucian seperti pada wanita

Periksa bahwa tutup rapat dan tidak pecah dan

Jika tak segera diperiksa almari es

CARA PEMBERIAN LABEL Baca pedoman Umum Cara Pemberian Label Catat apakah penderita telah mendapat

antibiotk CATATAN pd anak pakai Pediatric bag

PEMILIHAN Cara ini terbebas dari1048774pencemar uretra dan1048774perineum Diutamakan untuk1048774anak atau1048774pemeriksaan anaerobik PENGAMBILAN SPESIMEN1048774Bahan yang Dibutuhkan1048774Desinfektan kulit1048774Anastesi lokal1048774Semprit isi 10 ml dan jarum nomor 221048774Botol steril bertutup ulir

CARA PENGAMBILAN SPESIMEN Desinfeksi kulit Antar Pusar (Umbilicus) sd penis Anastesi pada tempat tusukan

( Mid-line 2 CM di atas simpisis) Masukkan jarum ke k kemih yang sedang penuh Hisap tampung dalam botol dan tutup rapat

CARA PEMBERIAN LABEL Baca Pedoman Umum Cara Pemberian Label Beri catatan1048774Urin suprapubik1048774Apakah perlu pemeriksaan anaerobik1048774Catat waktu pengambilan (Jam tanggal)

PENGIRIMAN Segera periksa dalam 30 menit atau taruh dalam almari es dan paling lama 24

jam

CATATAN Pemeriksaan anaerobik hanya atas

permintaan Anak tempat tusukan 1-2 CM di atas simpisis

pubis Jml 5 ml

BILA JUMLAH KOLONI ML URIN

lt10000 DIANGGAP KONTAMINASI

10000-100000 SANGSI PERLU DIULANG 100000 SIGNIFICANT BACTERIURIA

(JELAS UTI)

Kriteria klasifikasi UTI berdasarkan sindroma klinik

Category Clinical laboratory

Acute Uncomplicated UTI in women

bullDysuria urgencyfrequencysuprapubic painbullNo urinary symptom in last 4 wk before current episodebullNo fever or flank pain

ge10 WBCmm3

ge 103 CFUml uropathogens in CCMS urine

Acute uncomplicated pyelonephritis

bullFever chillsbullFlank pain on examinationbullOther diagnosis exludedbullNo history or clinical evidence of uropathologic abnormalities

ge10 WBCmm3

ge 104 CFUml uropathogens in CCMS urine

Complicated UTI and UTI in men

bullAny combination of symptom listed abovebullOne or more factors assosiated with complicated UTI

ge10 WBCmm3

ge 105 CFUml uropathogens in CCMS urine

Asymptomatic UTI bullNo urinary symptoms plusmngt10 WBCmm3

ge 105 CFUml in two CCMS cultures gt24 hrs apart

Factors assosiated with complicated UTI include any UTIin male indwelling or intermitten urinary catheter gt100ml of postvoided residual urine obstructionuropathy urologic abnormalities azotemia amp renal transplantationCCMS Clean Catch Midstream urine

Pedoman umum interpretasi kultur urin

Result Spsific specimen typeassociated clinical condition if known

ge104 CFUml of single potential pathogen or for each of two potential pathogens

CCMS urinepyellonephritis acute cystitis asymptomatic bacteriuria or catheterized urines

complete

ge103 CFUml of single potential pathogen

CCMS urineasymptomatic males or catheterized urines or acute urethral syndrome

Complete

ge three organisme types with no predominating organism

CCMS urine or catheterized urines

None because of possible contamination ask for another specimen

Either two or three organism types with predominant growth of one organism type and lt104 CFUml of the other organism type(s)

CCMS urine Complete workup for predominating oganism(s) description for the other organism(S)

ge102 CFUml of any number of organism types (set up with a 0001-and 001-ml calibrated loop)

Suprapubic aspirates any other surgically obtained urines (including ileal conduits cystoscopy specimens)

Complete

CFU colony forming unit Predominant growth= 104 to ge105 CFUml A complete workup includes indentification of organism and appropriate susceptibility testing

Page 4: dr.Iva Puspitasari,Sp.MK

INFORMASI UMUM Urin normal adalah steril Pencemaran uretra atau periuretra Urin kumpulan 24 jam Tidak bolehJENIS SPESIMEN URIN Urin kateter Urin porsi tengah ( (lsquoClean Catch Urinersquo) Urin Aspirasi Suprapubik

PEMILIHAN Kateter resiko memasukkan bakteri Jangan pakai Bed side Catheter bag PENGAMBILAN SPESIMEN II1 Bahan yang dibutuhkan Semprit isi 10 ml dan Jarum suntik nomor 21 Kapas alkohol

helliphellipUrine kateter

II2 Cara Pengambilan Sampel Jepit kateter (lt30 menit) Bersihkan dgn Alkohol pd tempat ambil urin Tusukkan jarum ambil urin tampung tutup

rapat CARA PEMBERIAN LABEL Pedoman Umum Cara Pemberian Label Cantumkan cara pengambilan urin misalnya Kateter

III PENGIRIMAN Segera priksa dalam 30 menit atau taruh dalam almari es dan paling lama 24 jam

URIN PORSI TENGAH(lsquoCLEAN CATCH URINErsquo)

I PEMILIHAN Dianjurkan urin pagi hari Buang 13 aliran urin pertama

PENGAMBILANII1 Bahan yang dibutuhkan

BotolTabung steril bertutup ulir Sabun medis Kasa Akuadesair suling

CARA PENGAMBILAN SPESIMENPenderita diberitahu (Lisantertulis) Instruksi pada Wanita1048774Duduk di toilet1048774Buka kakilutut ke samping selebar mungkin1048774Dgn sabun medis amp sponkainkapas cuci genital dgn gerak dari depan ke belakang1048774Bilas dengan spon basah depan ke belakang1048774Ulangi beberapa kali dgn spon basah baru1048774Pegang dengan jari dan taruh cawanbotol mulut

lebar di depan genital dan jangan menyentuhtepi botol

1048774Buang urin pertama keluar dan1048774Berikutnya ditampung1048774Tutup segera botol

Instruksi pada Laki Tarik kulit preputium (lsquoForeskinrsquo untuk yang

tidak khitan) dan bersihkan Glans penis Ikuti cara pencucian seperti pada wanita

Periksa bahwa tutup rapat dan tidak pecah dan

Jika tak segera diperiksa almari es

CARA PEMBERIAN LABEL Baca pedoman Umum Cara Pemberian Label Catat apakah penderita telah mendapat

antibiotk CATATAN pd anak pakai Pediatric bag

PEMILIHAN Cara ini terbebas dari1048774pencemar uretra dan1048774perineum Diutamakan untuk1048774anak atau1048774pemeriksaan anaerobik PENGAMBILAN SPESIMEN1048774Bahan yang Dibutuhkan1048774Desinfektan kulit1048774Anastesi lokal1048774Semprit isi 10 ml dan jarum nomor 221048774Botol steril bertutup ulir

CARA PENGAMBILAN SPESIMEN Desinfeksi kulit Antar Pusar (Umbilicus) sd penis Anastesi pada tempat tusukan

( Mid-line 2 CM di atas simpisis) Masukkan jarum ke k kemih yang sedang penuh Hisap tampung dalam botol dan tutup rapat

CARA PEMBERIAN LABEL Baca Pedoman Umum Cara Pemberian Label Beri catatan1048774Urin suprapubik1048774Apakah perlu pemeriksaan anaerobik1048774Catat waktu pengambilan (Jam tanggal)

PENGIRIMAN Segera periksa dalam 30 menit atau taruh dalam almari es dan paling lama 24

jam

CATATAN Pemeriksaan anaerobik hanya atas

permintaan Anak tempat tusukan 1-2 CM di atas simpisis

pubis Jml 5 ml

BILA JUMLAH KOLONI ML URIN

lt10000 DIANGGAP KONTAMINASI

10000-100000 SANGSI PERLU DIULANG 100000 SIGNIFICANT BACTERIURIA

(JELAS UTI)

Kriteria klasifikasi UTI berdasarkan sindroma klinik

Category Clinical laboratory

Acute Uncomplicated UTI in women

bullDysuria urgencyfrequencysuprapubic painbullNo urinary symptom in last 4 wk before current episodebullNo fever or flank pain

ge10 WBCmm3

ge 103 CFUml uropathogens in CCMS urine

Acute uncomplicated pyelonephritis

bullFever chillsbullFlank pain on examinationbullOther diagnosis exludedbullNo history or clinical evidence of uropathologic abnormalities

ge10 WBCmm3

ge 104 CFUml uropathogens in CCMS urine

Complicated UTI and UTI in men

bullAny combination of symptom listed abovebullOne or more factors assosiated with complicated UTI

ge10 WBCmm3

ge 105 CFUml uropathogens in CCMS urine

Asymptomatic UTI bullNo urinary symptoms plusmngt10 WBCmm3

ge 105 CFUml in two CCMS cultures gt24 hrs apart

Factors assosiated with complicated UTI include any UTIin male indwelling or intermitten urinary catheter gt100ml of postvoided residual urine obstructionuropathy urologic abnormalities azotemia amp renal transplantationCCMS Clean Catch Midstream urine

Pedoman umum interpretasi kultur urin

Result Spsific specimen typeassociated clinical condition if known

ge104 CFUml of single potential pathogen or for each of two potential pathogens

CCMS urinepyellonephritis acute cystitis asymptomatic bacteriuria or catheterized urines

complete

ge103 CFUml of single potential pathogen

CCMS urineasymptomatic males or catheterized urines or acute urethral syndrome

Complete

ge three organisme types with no predominating organism

CCMS urine or catheterized urines

None because of possible contamination ask for another specimen

Either two or three organism types with predominant growth of one organism type and lt104 CFUml of the other organism type(s)

CCMS urine Complete workup for predominating oganism(s) description for the other organism(S)

ge102 CFUml of any number of organism types (set up with a 0001-and 001-ml calibrated loop)

Suprapubic aspirates any other surgically obtained urines (including ileal conduits cystoscopy specimens)

Complete

CFU colony forming unit Predominant growth= 104 to ge105 CFUml A complete workup includes indentification of organism and appropriate susceptibility testing

Page 5: dr.Iva Puspitasari,Sp.MK

PEMILIHAN Kateter resiko memasukkan bakteri Jangan pakai Bed side Catheter bag PENGAMBILAN SPESIMEN II1 Bahan yang dibutuhkan Semprit isi 10 ml dan Jarum suntik nomor 21 Kapas alkohol

helliphellipUrine kateter

II2 Cara Pengambilan Sampel Jepit kateter (lt30 menit) Bersihkan dgn Alkohol pd tempat ambil urin Tusukkan jarum ambil urin tampung tutup

rapat CARA PEMBERIAN LABEL Pedoman Umum Cara Pemberian Label Cantumkan cara pengambilan urin misalnya Kateter

III PENGIRIMAN Segera priksa dalam 30 menit atau taruh dalam almari es dan paling lama 24 jam

URIN PORSI TENGAH(lsquoCLEAN CATCH URINErsquo)

I PEMILIHAN Dianjurkan urin pagi hari Buang 13 aliran urin pertama

PENGAMBILANII1 Bahan yang dibutuhkan

BotolTabung steril bertutup ulir Sabun medis Kasa Akuadesair suling

CARA PENGAMBILAN SPESIMENPenderita diberitahu (Lisantertulis) Instruksi pada Wanita1048774Duduk di toilet1048774Buka kakilutut ke samping selebar mungkin1048774Dgn sabun medis amp sponkainkapas cuci genital dgn gerak dari depan ke belakang1048774Bilas dengan spon basah depan ke belakang1048774Ulangi beberapa kali dgn spon basah baru1048774Pegang dengan jari dan taruh cawanbotol mulut

lebar di depan genital dan jangan menyentuhtepi botol

1048774Buang urin pertama keluar dan1048774Berikutnya ditampung1048774Tutup segera botol

Instruksi pada Laki Tarik kulit preputium (lsquoForeskinrsquo untuk yang

tidak khitan) dan bersihkan Glans penis Ikuti cara pencucian seperti pada wanita

Periksa bahwa tutup rapat dan tidak pecah dan

Jika tak segera diperiksa almari es

CARA PEMBERIAN LABEL Baca pedoman Umum Cara Pemberian Label Catat apakah penderita telah mendapat

antibiotk CATATAN pd anak pakai Pediatric bag

PEMILIHAN Cara ini terbebas dari1048774pencemar uretra dan1048774perineum Diutamakan untuk1048774anak atau1048774pemeriksaan anaerobik PENGAMBILAN SPESIMEN1048774Bahan yang Dibutuhkan1048774Desinfektan kulit1048774Anastesi lokal1048774Semprit isi 10 ml dan jarum nomor 221048774Botol steril bertutup ulir

CARA PENGAMBILAN SPESIMEN Desinfeksi kulit Antar Pusar (Umbilicus) sd penis Anastesi pada tempat tusukan

( Mid-line 2 CM di atas simpisis) Masukkan jarum ke k kemih yang sedang penuh Hisap tampung dalam botol dan tutup rapat

CARA PEMBERIAN LABEL Baca Pedoman Umum Cara Pemberian Label Beri catatan1048774Urin suprapubik1048774Apakah perlu pemeriksaan anaerobik1048774Catat waktu pengambilan (Jam tanggal)

PENGIRIMAN Segera periksa dalam 30 menit atau taruh dalam almari es dan paling lama 24

jam

CATATAN Pemeriksaan anaerobik hanya atas

permintaan Anak tempat tusukan 1-2 CM di atas simpisis

pubis Jml 5 ml

BILA JUMLAH KOLONI ML URIN

lt10000 DIANGGAP KONTAMINASI

10000-100000 SANGSI PERLU DIULANG 100000 SIGNIFICANT BACTERIURIA

(JELAS UTI)

Kriteria klasifikasi UTI berdasarkan sindroma klinik

Category Clinical laboratory

Acute Uncomplicated UTI in women

bullDysuria urgencyfrequencysuprapubic painbullNo urinary symptom in last 4 wk before current episodebullNo fever or flank pain

ge10 WBCmm3

ge 103 CFUml uropathogens in CCMS urine

Acute uncomplicated pyelonephritis

bullFever chillsbullFlank pain on examinationbullOther diagnosis exludedbullNo history or clinical evidence of uropathologic abnormalities

ge10 WBCmm3

ge 104 CFUml uropathogens in CCMS urine

Complicated UTI and UTI in men

bullAny combination of symptom listed abovebullOne or more factors assosiated with complicated UTI

ge10 WBCmm3

ge 105 CFUml uropathogens in CCMS urine

Asymptomatic UTI bullNo urinary symptoms plusmngt10 WBCmm3

ge 105 CFUml in two CCMS cultures gt24 hrs apart

Factors assosiated with complicated UTI include any UTIin male indwelling or intermitten urinary catheter gt100ml of postvoided residual urine obstructionuropathy urologic abnormalities azotemia amp renal transplantationCCMS Clean Catch Midstream urine

Pedoman umum interpretasi kultur urin

Result Spsific specimen typeassociated clinical condition if known

ge104 CFUml of single potential pathogen or for each of two potential pathogens

CCMS urinepyellonephritis acute cystitis asymptomatic bacteriuria or catheterized urines

complete

ge103 CFUml of single potential pathogen

CCMS urineasymptomatic males or catheterized urines or acute urethral syndrome

Complete

ge three organisme types with no predominating organism

CCMS urine or catheterized urines

None because of possible contamination ask for another specimen

Either two or three organism types with predominant growth of one organism type and lt104 CFUml of the other organism type(s)

CCMS urine Complete workup for predominating oganism(s) description for the other organism(S)

ge102 CFUml of any number of organism types (set up with a 0001-and 001-ml calibrated loop)

Suprapubic aspirates any other surgically obtained urines (including ileal conduits cystoscopy specimens)

Complete

CFU colony forming unit Predominant growth= 104 to ge105 CFUml A complete workup includes indentification of organism and appropriate susceptibility testing

Page 6: dr.Iva Puspitasari,Sp.MK

helliphellipUrine kateter

II2 Cara Pengambilan Sampel Jepit kateter (lt30 menit) Bersihkan dgn Alkohol pd tempat ambil urin Tusukkan jarum ambil urin tampung tutup

rapat CARA PEMBERIAN LABEL Pedoman Umum Cara Pemberian Label Cantumkan cara pengambilan urin misalnya Kateter

III PENGIRIMAN Segera priksa dalam 30 menit atau taruh dalam almari es dan paling lama 24 jam

URIN PORSI TENGAH(lsquoCLEAN CATCH URINErsquo)

I PEMILIHAN Dianjurkan urin pagi hari Buang 13 aliran urin pertama

PENGAMBILANII1 Bahan yang dibutuhkan

BotolTabung steril bertutup ulir Sabun medis Kasa Akuadesair suling

CARA PENGAMBILAN SPESIMENPenderita diberitahu (Lisantertulis) Instruksi pada Wanita1048774Duduk di toilet1048774Buka kakilutut ke samping selebar mungkin1048774Dgn sabun medis amp sponkainkapas cuci genital dgn gerak dari depan ke belakang1048774Bilas dengan spon basah depan ke belakang1048774Ulangi beberapa kali dgn spon basah baru1048774Pegang dengan jari dan taruh cawanbotol mulut

lebar di depan genital dan jangan menyentuhtepi botol

1048774Buang urin pertama keluar dan1048774Berikutnya ditampung1048774Tutup segera botol

Instruksi pada Laki Tarik kulit preputium (lsquoForeskinrsquo untuk yang

tidak khitan) dan bersihkan Glans penis Ikuti cara pencucian seperti pada wanita

Periksa bahwa tutup rapat dan tidak pecah dan

Jika tak segera diperiksa almari es

CARA PEMBERIAN LABEL Baca pedoman Umum Cara Pemberian Label Catat apakah penderita telah mendapat

antibiotk CATATAN pd anak pakai Pediatric bag

PEMILIHAN Cara ini terbebas dari1048774pencemar uretra dan1048774perineum Diutamakan untuk1048774anak atau1048774pemeriksaan anaerobik PENGAMBILAN SPESIMEN1048774Bahan yang Dibutuhkan1048774Desinfektan kulit1048774Anastesi lokal1048774Semprit isi 10 ml dan jarum nomor 221048774Botol steril bertutup ulir

CARA PENGAMBILAN SPESIMEN Desinfeksi kulit Antar Pusar (Umbilicus) sd penis Anastesi pada tempat tusukan

( Mid-line 2 CM di atas simpisis) Masukkan jarum ke k kemih yang sedang penuh Hisap tampung dalam botol dan tutup rapat

CARA PEMBERIAN LABEL Baca Pedoman Umum Cara Pemberian Label Beri catatan1048774Urin suprapubik1048774Apakah perlu pemeriksaan anaerobik1048774Catat waktu pengambilan (Jam tanggal)

PENGIRIMAN Segera periksa dalam 30 menit atau taruh dalam almari es dan paling lama 24

jam

CATATAN Pemeriksaan anaerobik hanya atas

permintaan Anak tempat tusukan 1-2 CM di atas simpisis

pubis Jml 5 ml

BILA JUMLAH KOLONI ML URIN

lt10000 DIANGGAP KONTAMINASI

10000-100000 SANGSI PERLU DIULANG 100000 SIGNIFICANT BACTERIURIA

(JELAS UTI)

Kriteria klasifikasi UTI berdasarkan sindroma klinik

Category Clinical laboratory

Acute Uncomplicated UTI in women

bullDysuria urgencyfrequencysuprapubic painbullNo urinary symptom in last 4 wk before current episodebullNo fever or flank pain

ge10 WBCmm3

ge 103 CFUml uropathogens in CCMS urine

Acute uncomplicated pyelonephritis

bullFever chillsbullFlank pain on examinationbullOther diagnosis exludedbullNo history or clinical evidence of uropathologic abnormalities

ge10 WBCmm3

ge 104 CFUml uropathogens in CCMS urine

Complicated UTI and UTI in men

bullAny combination of symptom listed abovebullOne or more factors assosiated with complicated UTI

ge10 WBCmm3

ge 105 CFUml uropathogens in CCMS urine

Asymptomatic UTI bullNo urinary symptoms plusmngt10 WBCmm3

ge 105 CFUml in two CCMS cultures gt24 hrs apart

Factors assosiated with complicated UTI include any UTIin male indwelling or intermitten urinary catheter gt100ml of postvoided residual urine obstructionuropathy urologic abnormalities azotemia amp renal transplantationCCMS Clean Catch Midstream urine

Pedoman umum interpretasi kultur urin

Result Spsific specimen typeassociated clinical condition if known

ge104 CFUml of single potential pathogen or for each of two potential pathogens

CCMS urinepyellonephritis acute cystitis asymptomatic bacteriuria or catheterized urines

complete

ge103 CFUml of single potential pathogen

CCMS urineasymptomatic males or catheterized urines or acute urethral syndrome

Complete

ge three organisme types with no predominating organism

CCMS urine or catheterized urines

None because of possible contamination ask for another specimen

Either two or three organism types with predominant growth of one organism type and lt104 CFUml of the other organism type(s)

CCMS urine Complete workup for predominating oganism(s) description for the other organism(S)

ge102 CFUml of any number of organism types (set up with a 0001-and 001-ml calibrated loop)

Suprapubic aspirates any other surgically obtained urines (including ileal conduits cystoscopy specimens)

Complete

CFU colony forming unit Predominant growth= 104 to ge105 CFUml A complete workup includes indentification of organism and appropriate susceptibility testing

Page 7: dr.Iva Puspitasari,Sp.MK

URIN PORSI TENGAH(lsquoCLEAN CATCH URINErsquo)

I PEMILIHAN Dianjurkan urin pagi hari Buang 13 aliran urin pertama

PENGAMBILANII1 Bahan yang dibutuhkan

BotolTabung steril bertutup ulir Sabun medis Kasa Akuadesair suling

CARA PENGAMBILAN SPESIMENPenderita diberitahu (Lisantertulis) Instruksi pada Wanita1048774Duduk di toilet1048774Buka kakilutut ke samping selebar mungkin1048774Dgn sabun medis amp sponkainkapas cuci genital dgn gerak dari depan ke belakang1048774Bilas dengan spon basah depan ke belakang1048774Ulangi beberapa kali dgn spon basah baru1048774Pegang dengan jari dan taruh cawanbotol mulut

lebar di depan genital dan jangan menyentuhtepi botol

1048774Buang urin pertama keluar dan1048774Berikutnya ditampung1048774Tutup segera botol

Instruksi pada Laki Tarik kulit preputium (lsquoForeskinrsquo untuk yang

tidak khitan) dan bersihkan Glans penis Ikuti cara pencucian seperti pada wanita

Periksa bahwa tutup rapat dan tidak pecah dan

Jika tak segera diperiksa almari es

CARA PEMBERIAN LABEL Baca pedoman Umum Cara Pemberian Label Catat apakah penderita telah mendapat

antibiotk CATATAN pd anak pakai Pediatric bag

PEMILIHAN Cara ini terbebas dari1048774pencemar uretra dan1048774perineum Diutamakan untuk1048774anak atau1048774pemeriksaan anaerobik PENGAMBILAN SPESIMEN1048774Bahan yang Dibutuhkan1048774Desinfektan kulit1048774Anastesi lokal1048774Semprit isi 10 ml dan jarum nomor 221048774Botol steril bertutup ulir

CARA PENGAMBILAN SPESIMEN Desinfeksi kulit Antar Pusar (Umbilicus) sd penis Anastesi pada tempat tusukan

( Mid-line 2 CM di atas simpisis) Masukkan jarum ke k kemih yang sedang penuh Hisap tampung dalam botol dan tutup rapat

CARA PEMBERIAN LABEL Baca Pedoman Umum Cara Pemberian Label Beri catatan1048774Urin suprapubik1048774Apakah perlu pemeriksaan anaerobik1048774Catat waktu pengambilan (Jam tanggal)

PENGIRIMAN Segera periksa dalam 30 menit atau taruh dalam almari es dan paling lama 24

jam

CATATAN Pemeriksaan anaerobik hanya atas

permintaan Anak tempat tusukan 1-2 CM di atas simpisis

pubis Jml 5 ml

BILA JUMLAH KOLONI ML URIN

lt10000 DIANGGAP KONTAMINASI

10000-100000 SANGSI PERLU DIULANG 100000 SIGNIFICANT BACTERIURIA

(JELAS UTI)

Kriteria klasifikasi UTI berdasarkan sindroma klinik

Category Clinical laboratory

Acute Uncomplicated UTI in women

bullDysuria urgencyfrequencysuprapubic painbullNo urinary symptom in last 4 wk before current episodebullNo fever or flank pain

ge10 WBCmm3

ge 103 CFUml uropathogens in CCMS urine

Acute uncomplicated pyelonephritis

bullFever chillsbullFlank pain on examinationbullOther diagnosis exludedbullNo history or clinical evidence of uropathologic abnormalities

ge10 WBCmm3

ge 104 CFUml uropathogens in CCMS urine

Complicated UTI and UTI in men

bullAny combination of symptom listed abovebullOne or more factors assosiated with complicated UTI

ge10 WBCmm3

ge 105 CFUml uropathogens in CCMS urine

Asymptomatic UTI bullNo urinary symptoms plusmngt10 WBCmm3

ge 105 CFUml in two CCMS cultures gt24 hrs apart

Factors assosiated with complicated UTI include any UTIin male indwelling or intermitten urinary catheter gt100ml of postvoided residual urine obstructionuropathy urologic abnormalities azotemia amp renal transplantationCCMS Clean Catch Midstream urine

Pedoman umum interpretasi kultur urin

Result Spsific specimen typeassociated clinical condition if known

ge104 CFUml of single potential pathogen or for each of two potential pathogens

CCMS urinepyellonephritis acute cystitis asymptomatic bacteriuria or catheterized urines

complete

ge103 CFUml of single potential pathogen

CCMS urineasymptomatic males or catheterized urines or acute urethral syndrome

Complete

ge three organisme types with no predominating organism

CCMS urine or catheterized urines

None because of possible contamination ask for another specimen

Either two or three organism types with predominant growth of one organism type and lt104 CFUml of the other organism type(s)

CCMS urine Complete workup for predominating oganism(s) description for the other organism(S)

ge102 CFUml of any number of organism types (set up with a 0001-and 001-ml calibrated loop)

Suprapubic aspirates any other surgically obtained urines (including ileal conduits cystoscopy specimens)

Complete

CFU colony forming unit Predominant growth= 104 to ge105 CFUml A complete workup includes indentification of organism and appropriate susceptibility testing

Page 8: dr.Iva Puspitasari,Sp.MK

CARA PENGAMBILAN SPESIMENPenderita diberitahu (Lisantertulis) Instruksi pada Wanita1048774Duduk di toilet1048774Buka kakilutut ke samping selebar mungkin1048774Dgn sabun medis amp sponkainkapas cuci genital dgn gerak dari depan ke belakang1048774Bilas dengan spon basah depan ke belakang1048774Ulangi beberapa kali dgn spon basah baru1048774Pegang dengan jari dan taruh cawanbotol mulut

lebar di depan genital dan jangan menyentuhtepi botol

1048774Buang urin pertama keluar dan1048774Berikutnya ditampung1048774Tutup segera botol

Instruksi pada Laki Tarik kulit preputium (lsquoForeskinrsquo untuk yang

tidak khitan) dan bersihkan Glans penis Ikuti cara pencucian seperti pada wanita

Periksa bahwa tutup rapat dan tidak pecah dan

Jika tak segera diperiksa almari es

CARA PEMBERIAN LABEL Baca pedoman Umum Cara Pemberian Label Catat apakah penderita telah mendapat

antibiotk CATATAN pd anak pakai Pediatric bag

PEMILIHAN Cara ini terbebas dari1048774pencemar uretra dan1048774perineum Diutamakan untuk1048774anak atau1048774pemeriksaan anaerobik PENGAMBILAN SPESIMEN1048774Bahan yang Dibutuhkan1048774Desinfektan kulit1048774Anastesi lokal1048774Semprit isi 10 ml dan jarum nomor 221048774Botol steril bertutup ulir

CARA PENGAMBILAN SPESIMEN Desinfeksi kulit Antar Pusar (Umbilicus) sd penis Anastesi pada tempat tusukan

( Mid-line 2 CM di atas simpisis) Masukkan jarum ke k kemih yang sedang penuh Hisap tampung dalam botol dan tutup rapat

CARA PEMBERIAN LABEL Baca Pedoman Umum Cara Pemberian Label Beri catatan1048774Urin suprapubik1048774Apakah perlu pemeriksaan anaerobik1048774Catat waktu pengambilan (Jam tanggal)

PENGIRIMAN Segera periksa dalam 30 menit atau taruh dalam almari es dan paling lama 24

jam

CATATAN Pemeriksaan anaerobik hanya atas

permintaan Anak tempat tusukan 1-2 CM di atas simpisis

pubis Jml 5 ml

BILA JUMLAH KOLONI ML URIN

lt10000 DIANGGAP KONTAMINASI

10000-100000 SANGSI PERLU DIULANG 100000 SIGNIFICANT BACTERIURIA

(JELAS UTI)

Kriteria klasifikasi UTI berdasarkan sindroma klinik

Category Clinical laboratory

Acute Uncomplicated UTI in women

bullDysuria urgencyfrequencysuprapubic painbullNo urinary symptom in last 4 wk before current episodebullNo fever or flank pain

ge10 WBCmm3

ge 103 CFUml uropathogens in CCMS urine

Acute uncomplicated pyelonephritis

bullFever chillsbullFlank pain on examinationbullOther diagnosis exludedbullNo history or clinical evidence of uropathologic abnormalities

ge10 WBCmm3

ge 104 CFUml uropathogens in CCMS urine

Complicated UTI and UTI in men

bullAny combination of symptom listed abovebullOne or more factors assosiated with complicated UTI

ge10 WBCmm3

ge 105 CFUml uropathogens in CCMS urine

Asymptomatic UTI bullNo urinary symptoms plusmngt10 WBCmm3

ge 105 CFUml in two CCMS cultures gt24 hrs apart

Factors assosiated with complicated UTI include any UTIin male indwelling or intermitten urinary catheter gt100ml of postvoided residual urine obstructionuropathy urologic abnormalities azotemia amp renal transplantationCCMS Clean Catch Midstream urine

Pedoman umum interpretasi kultur urin

Result Spsific specimen typeassociated clinical condition if known

ge104 CFUml of single potential pathogen or for each of two potential pathogens

CCMS urinepyellonephritis acute cystitis asymptomatic bacteriuria or catheterized urines

complete

ge103 CFUml of single potential pathogen

CCMS urineasymptomatic males or catheterized urines or acute urethral syndrome

Complete

ge three organisme types with no predominating organism

CCMS urine or catheterized urines

None because of possible contamination ask for another specimen

Either two or three organism types with predominant growth of one organism type and lt104 CFUml of the other organism type(s)

CCMS urine Complete workup for predominating oganism(s) description for the other organism(S)

ge102 CFUml of any number of organism types (set up with a 0001-and 001-ml calibrated loop)

Suprapubic aspirates any other surgically obtained urines (including ileal conduits cystoscopy specimens)

Complete

CFU colony forming unit Predominant growth= 104 to ge105 CFUml A complete workup includes indentification of organism and appropriate susceptibility testing

Page 9: dr.Iva Puspitasari,Sp.MK

Instruksi pada Laki Tarik kulit preputium (lsquoForeskinrsquo untuk yang

tidak khitan) dan bersihkan Glans penis Ikuti cara pencucian seperti pada wanita

Periksa bahwa tutup rapat dan tidak pecah dan

Jika tak segera diperiksa almari es

CARA PEMBERIAN LABEL Baca pedoman Umum Cara Pemberian Label Catat apakah penderita telah mendapat

antibiotk CATATAN pd anak pakai Pediatric bag

PEMILIHAN Cara ini terbebas dari1048774pencemar uretra dan1048774perineum Diutamakan untuk1048774anak atau1048774pemeriksaan anaerobik PENGAMBILAN SPESIMEN1048774Bahan yang Dibutuhkan1048774Desinfektan kulit1048774Anastesi lokal1048774Semprit isi 10 ml dan jarum nomor 221048774Botol steril bertutup ulir

CARA PENGAMBILAN SPESIMEN Desinfeksi kulit Antar Pusar (Umbilicus) sd penis Anastesi pada tempat tusukan

( Mid-line 2 CM di atas simpisis) Masukkan jarum ke k kemih yang sedang penuh Hisap tampung dalam botol dan tutup rapat

CARA PEMBERIAN LABEL Baca Pedoman Umum Cara Pemberian Label Beri catatan1048774Urin suprapubik1048774Apakah perlu pemeriksaan anaerobik1048774Catat waktu pengambilan (Jam tanggal)

PENGIRIMAN Segera periksa dalam 30 menit atau taruh dalam almari es dan paling lama 24

jam

CATATAN Pemeriksaan anaerobik hanya atas

permintaan Anak tempat tusukan 1-2 CM di atas simpisis

pubis Jml 5 ml

BILA JUMLAH KOLONI ML URIN

lt10000 DIANGGAP KONTAMINASI

10000-100000 SANGSI PERLU DIULANG 100000 SIGNIFICANT BACTERIURIA

(JELAS UTI)

Kriteria klasifikasi UTI berdasarkan sindroma klinik

Category Clinical laboratory

Acute Uncomplicated UTI in women

bullDysuria urgencyfrequencysuprapubic painbullNo urinary symptom in last 4 wk before current episodebullNo fever or flank pain

ge10 WBCmm3

ge 103 CFUml uropathogens in CCMS urine

Acute uncomplicated pyelonephritis

bullFever chillsbullFlank pain on examinationbullOther diagnosis exludedbullNo history or clinical evidence of uropathologic abnormalities

ge10 WBCmm3

ge 104 CFUml uropathogens in CCMS urine

Complicated UTI and UTI in men

bullAny combination of symptom listed abovebullOne or more factors assosiated with complicated UTI

ge10 WBCmm3

ge 105 CFUml uropathogens in CCMS urine

Asymptomatic UTI bullNo urinary symptoms plusmngt10 WBCmm3

ge 105 CFUml in two CCMS cultures gt24 hrs apart

Factors assosiated with complicated UTI include any UTIin male indwelling or intermitten urinary catheter gt100ml of postvoided residual urine obstructionuropathy urologic abnormalities azotemia amp renal transplantationCCMS Clean Catch Midstream urine

Pedoman umum interpretasi kultur urin

Result Spsific specimen typeassociated clinical condition if known

ge104 CFUml of single potential pathogen or for each of two potential pathogens

CCMS urinepyellonephritis acute cystitis asymptomatic bacteriuria or catheterized urines

complete

ge103 CFUml of single potential pathogen

CCMS urineasymptomatic males or catheterized urines or acute urethral syndrome

Complete

ge three organisme types with no predominating organism

CCMS urine or catheterized urines

None because of possible contamination ask for another specimen

Either two or three organism types with predominant growth of one organism type and lt104 CFUml of the other organism type(s)

CCMS urine Complete workup for predominating oganism(s) description for the other organism(S)

ge102 CFUml of any number of organism types (set up with a 0001-and 001-ml calibrated loop)

Suprapubic aspirates any other surgically obtained urines (including ileal conduits cystoscopy specimens)

Complete

CFU colony forming unit Predominant growth= 104 to ge105 CFUml A complete workup includes indentification of organism and appropriate susceptibility testing

Page 10: dr.Iva Puspitasari,Sp.MK

PEMILIHAN Cara ini terbebas dari1048774pencemar uretra dan1048774perineum Diutamakan untuk1048774anak atau1048774pemeriksaan anaerobik PENGAMBILAN SPESIMEN1048774Bahan yang Dibutuhkan1048774Desinfektan kulit1048774Anastesi lokal1048774Semprit isi 10 ml dan jarum nomor 221048774Botol steril bertutup ulir

CARA PENGAMBILAN SPESIMEN Desinfeksi kulit Antar Pusar (Umbilicus) sd penis Anastesi pada tempat tusukan

( Mid-line 2 CM di atas simpisis) Masukkan jarum ke k kemih yang sedang penuh Hisap tampung dalam botol dan tutup rapat

CARA PEMBERIAN LABEL Baca Pedoman Umum Cara Pemberian Label Beri catatan1048774Urin suprapubik1048774Apakah perlu pemeriksaan anaerobik1048774Catat waktu pengambilan (Jam tanggal)

PENGIRIMAN Segera periksa dalam 30 menit atau taruh dalam almari es dan paling lama 24

jam

CATATAN Pemeriksaan anaerobik hanya atas

permintaan Anak tempat tusukan 1-2 CM di atas simpisis

pubis Jml 5 ml

BILA JUMLAH KOLONI ML URIN

lt10000 DIANGGAP KONTAMINASI

10000-100000 SANGSI PERLU DIULANG 100000 SIGNIFICANT BACTERIURIA

(JELAS UTI)

Kriteria klasifikasi UTI berdasarkan sindroma klinik

Category Clinical laboratory

Acute Uncomplicated UTI in women

bullDysuria urgencyfrequencysuprapubic painbullNo urinary symptom in last 4 wk before current episodebullNo fever or flank pain

ge10 WBCmm3

ge 103 CFUml uropathogens in CCMS urine

Acute uncomplicated pyelonephritis

bullFever chillsbullFlank pain on examinationbullOther diagnosis exludedbullNo history or clinical evidence of uropathologic abnormalities

ge10 WBCmm3

ge 104 CFUml uropathogens in CCMS urine

Complicated UTI and UTI in men

bullAny combination of symptom listed abovebullOne or more factors assosiated with complicated UTI

ge10 WBCmm3

ge 105 CFUml uropathogens in CCMS urine

Asymptomatic UTI bullNo urinary symptoms plusmngt10 WBCmm3

ge 105 CFUml in two CCMS cultures gt24 hrs apart

Factors assosiated with complicated UTI include any UTIin male indwelling or intermitten urinary catheter gt100ml of postvoided residual urine obstructionuropathy urologic abnormalities azotemia amp renal transplantationCCMS Clean Catch Midstream urine

Pedoman umum interpretasi kultur urin

Result Spsific specimen typeassociated clinical condition if known

ge104 CFUml of single potential pathogen or for each of two potential pathogens

CCMS urinepyellonephritis acute cystitis asymptomatic bacteriuria or catheterized urines

complete

ge103 CFUml of single potential pathogen

CCMS urineasymptomatic males or catheterized urines or acute urethral syndrome

Complete

ge three organisme types with no predominating organism

CCMS urine or catheterized urines

None because of possible contamination ask for another specimen

Either two or three organism types with predominant growth of one organism type and lt104 CFUml of the other organism type(s)

CCMS urine Complete workup for predominating oganism(s) description for the other organism(S)

ge102 CFUml of any number of organism types (set up with a 0001-and 001-ml calibrated loop)

Suprapubic aspirates any other surgically obtained urines (including ileal conduits cystoscopy specimens)

Complete

CFU colony forming unit Predominant growth= 104 to ge105 CFUml A complete workup includes indentification of organism and appropriate susceptibility testing

Page 11: dr.Iva Puspitasari,Sp.MK

CARA PENGAMBILAN SPESIMEN Desinfeksi kulit Antar Pusar (Umbilicus) sd penis Anastesi pada tempat tusukan

( Mid-line 2 CM di atas simpisis) Masukkan jarum ke k kemih yang sedang penuh Hisap tampung dalam botol dan tutup rapat

CARA PEMBERIAN LABEL Baca Pedoman Umum Cara Pemberian Label Beri catatan1048774Urin suprapubik1048774Apakah perlu pemeriksaan anaerobik1048774Catat waktu pengambilan (Jam tanggal)

PENGIRIMAN Segera periksa dalam 30 menit atau taruh dalam almari es dan paling lama 24

jam

CATATAN Pemeriksaan anaerobik hanya atas

permintaan Anak tempat tusukan 1-2 CM di atas simpisis

pubis Jml 5 ml

BILA JUMLAH KOLONI ML URIN

lt10000 DIANGGAP KONTAMINASI

10000-100000 SANGSI PERLU DIULANG 100000 SIGNIFICANT BACTERIURIA

(JELAS UTI)

Kriteria klasifikasi UTI berdasarkan sindroma klinik

Category Clinical laboratory

Acute Uncomplicated UTI in women

bullDysuria urgencyfrequencysuprapubic painbullNo urinary symptom in last 4 wk before current episodebullNo fever or flank pain

ge10 WBCmm3

ge 103 CFUml uropathogens in CCMS urine

Acute uncomplicated pyelonephritis

bullFever chillsbullFlank pain on examinationbullOther diagnosis exludedbullNo history or clinical evidence of uropathologic abnormalities

ge10 WBCmm3

ge 104 CFUml uropathogens in CCMS urine

Complicated UTI and UTI in men

bullAny combination of symptom listed abovebullOne or more factors assosiated with complicated UTI

ge10 WBCmm3

ge 105 CFUml uropathogens in CCMS urine

Asymptomatic UTI bullNo urinary symptoms plusmngt10 WBCmm3

ge 105 CFUml in two CCMS cultures gt24 hrs apart

Factors assosiated with complicated UTI include any UTIin male indwelling or intermitten urinary catheter gt100ml of postvoided residual urine obstructionuropathy urologic abnormalities azotemia amp renal transplantationCCMS Clean Catch Midstream urine

Pedoman umum interpretasi kultur urin

Result Spsific specimen typeassociated clinical condition if known

ge104 CFUml of single potential pathogen or for each of two potential pathogens

CCMS urinepyellonephritis acute cystitis asymptomatic bacteriuria or catheterized urines

complete

ge103 CFUml of single potential pathogen

CCMS urineasymptomatic males or catheterized urines or acute urethral syndrome

Complete

ge three organisme types with no predominating organism

CCMS urine or catheterized urines

None because of possible contamination ask for another specimen

Either two or three organism types with predominant growth of one organism type and lt104 CFUml of the other organism type(s)

CCMS urine Complete workup for predominating oganism(s) description for the other organism(S)

ge102 CFUml of any number of organism types (set up with a 0001-and 001-ml calibrated loop)

Suprapubic aspirates any other surgically obtained urines (including ileal conduits cystoscopy specimens)

Complete

CFU colony forming unit Predominant growth= 104 to ge105 CFUml A complete workup includes indentification of organism and appropriate susceptibility testing

Page 12: dr.Iva Puspitasari,Sp.MK

PENGIRIMAN Segera periksa dalam 30 menit atau taruh dalam almari es dan paling lama 24

jam

CATATAN Pemeriksaan anaerobik hanya atas

permintaan Anak tempat tusukan 1-2 CM di atas simpisis

pubis Jml 5 ml

BILA JUMLAH KOLONI ML URIN

lt10000 DIANGGAP KONTAMINASI

10000-100000 SANGSI PERLU DIULANG 100000 SIGNIFICANT BACTERIURIA

(JELAS UTI)

Kriteria klasifikasi UTI berdasarkan sindroma klinik

Category Clinical laboratory

Acute Uncomplicated UTI in women

bullDysuria urgencyfrequencysuprapubic painbullNo urinary symptom in last 4 wk before current episodebullNo fever or flank pain

ge10 WBCmm3

ge 103 CFUml uropathogens in CCMS urine

Acute uncomplicated pyelonephritis

bullFever chillsbullFlank pain on examinationbullOther diagnosis exludedbullNo history or clinical evidence of uropathologic abnormalities

ge10 WBCmm3

ge 104 CFUml uropathogens in CCMS urine

Complicated UTI and UTI in men

bullAny combination of symptom listed abovebullOne or more factors assosiated with complicated UTI

ge10 WBCmm3

ge 105 CFUml uropathogens in CCMS urine

Asymptomatic UTI bullNo urinary symptoms plusmngt10 WBCmm3

ge 105 CFUml in two CCMS cultures gt24 hrs apart

Factors assosiated with complicated UTI include any UTIin male indwelling or intermitten urinary catheter gt100ml of postvoided residual urine obstructionuropathy urologic abnormalities azotemia amp renal transplantationCCMS Clean Catch Midstream urine

Pedoman umum interpretasi kultur urin

Result Spsific specimen typeassociated clinical condition if known

ge104 CFUml of single potential pathogen or for each of two potential pathogens

CCMS urinepyellonephritis acute cystitis asymptomatic bacteriuria or catheterized urines

complete

ge103 CFUml of single potential pathogen

CCMS urineasymptomatic males or catheterized urines or acute urethral syndrome

Complete

ge three organisme types with no predominating organism

CCMS urine or catheterized urines

None because of possible contamination ask for another specimen

Either two or three organism types with predominant growth of one organism type and lt104 CFUml of the other organism type(s)

CCMS urine Complete workup for predominating oganism(s) description for the other organism(S)

ge102 CFUml of any number of organism types (set up with a 0001-and 001-ml calibrated loop)

Suprapubic aspirates any other surgically obtained urines (including ileal conduits cystoscopy specimens)

Complete

CFU colony forming unit Predominant growth= 104 to ge105 CFUml A complete workup includes indentification of organism and appropriate susceptibility testing

Page 13: dr.Iva Puspitasari,Sp.MK

BILA JUMLAH KOLONI ML URIN

lt10000 DIANGGAP KONTAMINASI

10000-100000 SANGSI PERLU DIULANG 100000 SIGNIFICANT BACTERIURIA

(JELAS UTI)

Kriteria klasifikasi UTI berdasarkan sindroma klinik

Category Clinical laboratory

Acute Uncomplicated UTI in women

bullDysuria urgencyfrequencysuprapubic painbullNo urinary symptom in last 4 wk before current episodebullNo fever or flank pain

ge10 WBCmm3

ge 103 CFUml uropathogens in CCMS urine

Acute uncomplicated pyelonephritis

bullFever chillsbullFlank pain on examinationbullOther diagnosis exludedbullNo history or clinical evidence of uropathologic abnormalities

ge10 WBCmm3

ge 104 CFUml uropathogens in CCMS urine

Complicated UTI and UTI in men

bullAny combination of symptom listed abovebullOne or more factors assosiated with complicated UTI

ge10 WBCmm3

ge 105 CFUml uropathogens in CCMS urine

Asymptomatic UTI bullNo urinary symptoms plusmngt10 WBCmm3

ge 105 CFUml in two CCMS cultures gt24 hrs apart

Factors assosiated with complicated UTI include any UTIin male indwelling or intermitten urinary catheter gt100ml of postvoided residual urine obstructionuropathy urologic abnormalities azotemia amp renal transplantationCCMS Clean Catch Midstream urine

Pedoman umum interpretasi kultur urin

Result Spsific specimen typeassociated clinical condition if known

ge104 CFUml of single potential pathogen or for each of two potential pathogens

CCMS urinepyellonephritis acute cystitis asymptomatic bacteriuria or catheterized urines

complete

ge103 CFUml of single potential pathogen

CCMS urineasymptomatic males or catheterized urines or acute urethral syndrome

Complete

ge three organisme types with no predominating organism

CCMS urine or catheterized urines

None because of possible contamination ask for another specimen

Either two or three organism types with predominant growth of one organism type and lt104 CFUml of the other organism type(s)

CCMS urine Complete workup for predominating oganism(s) description for the other organism(S)

ge102 CFUml of any number of organism types (set up with a 0001-and 001-ml calibrated loop)

Suprapubic aspirates any other surgically obtained urines (including ileal conduits cystoscopy specimens)

Complete

CFU colony forming unit Predominant growth= 104 to ge105 CFUml A complete workup includes indentification of organism and appropriate susceptibility testing

Page 14: dr.Iva Puspitasari,Sp.MK

Kriteria klasifikasi UTI berdasarkan sindroma klinik

Category Clinical laboratory

Acute Uncomplicated UTI in women

bullDysuria urgencyfrequencysuprapubic painbullNo urinary symptom in last 4 wk before current episodebullNo fever or flank pain

ge10 WBCmm3

ge 103 CFUml uropathogens in CCMS urine

Acute uncomplicated pyelonephritis

bullFever chillsbullFlank pain on examinationbullOther diagnosis exludedbullNo history or clinical evidence of uropathologic abnormalities

ge10 WBCmm3

ge 104 CFUml uropathogens in CCMS urine

Complicated UTI and UTI in men

bullAny combination of symptom listed abovebullOne or more factors assosiated with complicated UTI

ge10 WBCmm3

ge 105 CFUml uropathogens in CCMS urine

Asymptomatic UTI bullNo urinary symptoms plusmngt10 WBCmm3

ge 105 CFUml in two CCMS cultures gt24 hrs apart

Factors assosiated with complicated UTI include any UTIin male indwelling or intermitten urinary catheter gt100ml of postvoided residual urine obstructionuropathy urologic abnormalities azotemia amp renal transplantationCCMS Clean Catch Midstream urine

Pedoman umum interpretasi kultur urin

Result Spsific specimen typeassociated clinical condition if known

ge104 CFUml of single potential pathogen or for each of two potential pathogens

CCMS urinepyellonephritis acute cystitis asymptomatic bacteriuria or catheterized urines

complete

ge103 CFUml of single potential pathogen

CCMS urineasymptomatic males or catheterized urines or acute urethral syndrome

Complete

ge three organisme types with no predominating organism

CCMS urine or catheterized urines

None because of possible contamination ask for another specimen

Either two or three organism types with predominant growth of one organism type and lt104 CFUml of the other organism type(s)

CCMS urine Complete workup for predominating oganism(s) description for the other organism(S)

ge102 CFUml of any number of organism types (set up with a 0001-and 001-ml calibrated loop)

Suprapubic aspirates any other surgically obtained urines (including ileal conduits cystoscopy specimens)

Complete

CFU colony forming unit Predominant growth= 104 to ge105 CFUml A complete workup includes indentification of organism and appropriate susceptibility testing

Page 15: dr.Iva Puspitasari,Sp.MK

Pedoman umum interpretasi kultur urin

Result Spsific specimen typeassociated clinical condition if known

ge104 CFUml of single potential pathogen or for each of two potential pathogens

CCMS urinepyellonephritis acute cystitis asymptomatic bacteriuria or catheterized urines

complete

ge103 CFUml of single potential pathogen

CCMS urineasymptomatic males or catheterized urines or acute urethral syndrome

Complete

ge three organisme types with no predominating organism

CCMS urine or catheterized urines

None because of possible contamination ask for another specimen

Either two or three organism types with predominant growth of one organism type and lt104 CFUml of the other organism type(s)

CCMS urine Complete workup for predominating oganism(s) description for the other organism(S)

ge102 CFUml of any number of organism types (set up with a 0001-and 001-ml calibrated loop)

Suprapubic aspirates any other surgically obtained urines (including ileal conduits cystoscopy specimens)

Complete

CFU colony forming unit Predominant growth= 104 to ge105 CFUml A complete workup includes indentification of organism and appropriate susceptibility testing