7. LAMPIRAN Lampiran 1. Kurva Standar Tokoferol … · Fermentasi Spontan Ul a b. 34. Fermenta si...
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28 7. LAMPIRAN Lampiran 1. Kurva Standar Tokoferol Tokoferol ppm abs 0 0.0392 40 0.1407 80 0.2509 120 0.3866 160 0.5098 200 0.6722 y = 0.0031x + 0.0184 R² = 0.9941 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0 50 100 150 200 250 Series1 Linear (Series1)
Transcript of 7. LAMPIRAN Lampiran 1. Kurva Standar Tokoferol … · Fermentasi Spontan Ul a b. 34. Fermenta si...
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7. LAMPIRAN
Lampiran 1. Kurva Standar Tokoferol
Tokoferol
ppm abs
0 0.0392
40 0.1407
80 0.2509
120 0.3866
160 0.5098
200 0.6722
y = 0.0031x + 0.0184 R² = 0.9941
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0 50 100 150 200 250
Series1
Linear (Series1)
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Lampiran 2. Kurva Standar Total Fenol
y = 0.0042x + 0.0369 R² = 0.9978
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
0 50 100 150 200 250
Series1
Linear (Series1)
Total Fenol
ppm abs
0 0.0414
50 0.2564
100 0.4265
150 0.6591
200 0.8792
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Lampiran 3. Pembuatan Media MRS broth
Medium MRS broth dibuat dengan mengencerkan 52,2 gram bubuk Merck MRS broth
dalam 1 liter aquades, kemudian medium diaduk hingga terlarut dengan merata. Setelah
itu medium disterilkan menggunakan autoclave pada suhu 121oC selama 15 menit.
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Lampiran 4. Data Absorbansi Tokoferol
Batch 1
32
Batch 2
33
Lampiran 5. Data Absorbansi Total Fenol
Batch 1
0 1 0.2011 0.036 0.004 25 10 10318.75 1031.875
2 0.2036 0.036 0.004 25 10 10475 1047.5
3 0.2006 0.036 0.004 25 10 10287.5 1028.75
4 0.2002 0.036 0.004 25 10 10262.5 1026.25
5 0.2037 0.036 0.004 25 10 10481.25 1048.125
6 0.2021 0.036 0.004 25 10 10381.25 1038.125
1 1 0.2051 0.036 0.004 25 10 10568.75 1056.875
2 0.2046 0.036 0.004 25 10 10537.5 1053.75
3 0.2076 0.036 0.004 25 10 10725 1072.5
4 0.2047 0.036 0.004 25 10 10543.75 1054.375
5 0.2034 0.036 0.004 25 10 10462.5 1046.25
6 0.2037 0.036 0.004 25 10 10481.25 1048.125
2 1 0.2132 0.036 0.004 25 10 11075 1107.5
2 0.2164 0.036 0.004 25 10 11275 1127.5
3 0.2148 0.036 0.004 25 10 11175 1117.5
4 0.2162 0.036 0.004 25 10 11262.5 1126.25
5 0.2157 0.036 0.004 25 10 11231.25 1123.125
6 0.2143 0.036 0.004 25 10 11143.75 1114.375
3 1 0.2214 0.036 0.004 25 10 11587.5 1158.75
2 0.221 0.036 0.004 25 10 11562.5 1156.25
3 0.2201 0.036 0.004 25 10 11506.25 1150.625
4 0.2206 0.036 0.004 25 10 11537.5 1153.75
5 0.2207 0.036 0.004 25 10 11543.75 1154.375
6 0.2218 0.036 0.004 25 10 11612.5 1161.25
4 1 0.2409 0.036 0.004 25 10 12806.25 1280.625
2 0.241 0.036 0.004 25 10 12812.5 1281.25
3 0.2401 0.036 0.004 25 10 12756.25 1275.625
4 0.2416 0.036 0.004 25 10 12850 1285
5 0.2389 0.036 0.004 25 10 12681.25 1268.125
6 0.2401 0.036 0.004 25 10 12756.25 1275.625
5 1 0.2504 0.036 0.004 25 10 13400 1340
2 0.2512 0.036 0.004 25 10 13450 1345
3 0.2503 0.036 0.004 25 10 13393.75 1339.375
4 0.2514 0.036 0.004 25 10 13462.5 1346.25
5 0.2525 0.036 0.004 25 10 13531.25 1353.125
6 0.2518 0.036 0.004 25 10 13487.5 1348.75
5.23907594
5.9116763
3.78456294
7.7055175 7.97%
11.48%
23.24%
29.77%
1277.70833
1345.41667
9.32696025
9.432462781036.77083
1055.3125
1119.375
1155.83333
0.00%
1.79%
StdevEkstrak
si
Pengen
ceran
Total Fenol
(ppm)
Rata2
T.Fenol
Total Fenol
(mg/100g)
%
Peningkatan
Hari
ke-
Fermentasi
Spontana bUl
34
Fermenta
si BALa b
Ekstra
ksi
Pengencera
n
Total Fenol
(ppm)
Total Fenol
(mg/100g)
Rata2
T.Fenol
(mg/100g)
stdev%
Peningkatan
0 1 0.2029 0.036 0.004 25 10 10431.25 1043.125
2 0.2031 0.036 0.004 25 10 10443.75 1044.375
3 0.2046 0.036 0.004 25 10 10537.5 1053.75
4 0.2029 0.036 0.004 25 10 10431.25 1043.125
5 0.2022 0.036 0.004 25 10 10387.5 1038.75
6 0.2033 0.036 0.004 25 10 10456.25 1045.625
1 1 0.2132 0.036 0.004 25 10 11075 1107.5
2 0.2122 0.036 0.004 25 10 11012.5 1101.25
3 0.2124 0.036 0.004 25 10 11025 1102.5
4 0.214 0.036 0.004 25 10 11125 1112.5
5 0.2108 0.036 0.004 25 10 10925 1092.5
6 0.2105 0.036 0.004 25 10 10906.25 1090.625
2 1 0.2295 0.036 0.004 25 10 12093.75 1209.375
2 0.2287 0.036 0.004 25 10 12043.75 1204.375
3 0.2268 0.036 0.004 25 10 11925 1192.5
4 0.2307 0.036 0.004 25 10 12168.75 1216.875
5 0.2304 0.036 0.004 25 10 12150 1215
6 0.231 0.036 0.004 25 10 12187.5 1218.75
3 1 0.2327 0.036 0.004 25 10 12293.75 1229.375
2 0.2325 0.036 0.004 25 10 12281.25 1228.125
3 0.2338 0.036 0.004 25 10 12362.5 1236.25
4 0.2333 0.036 0.004 25 10 12331.25 1233.125
5 0.232 0.036 0.004 25 10 12250 1225
6 0.2356 0.036 0.004 25 10 12475 1247.5
4 1 0.256 0.036 0.004 25 10 13750 1375
2 0.2564 0.036 0.004 25 10 13775 1377.5
3 0.2531 0.036 0.004 25 10 13568.75 1356.875
4 0.2573 0.036 0.004 25 10 13831.25 1383.125
5 0.2564 0.036 0.004 25 10 13775 1377.5
6 0.257 0.036 0.004 25 10 13812.5 1381.25
31.63%
1044.79167 4.9634
8.44481101.14583
1209.47917 9.8537
8.01771233.22917 18.04%
0.00%
5.39%
15.76%
9.44281375.20833
UlHari
ke-
35
Batch 2
36
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Lampiran 6. Analisis Data Tokoferol Fermentasi Spontan
Case Process ing Sum mary
4 100.0% 0 .0% 4 100.0%
4 100.0% 0 .0% 4 100.0%
4 100.0% 0 .0% 4 100.0%
4 100.0% 0 .0% 4 100.0%
4 100.0% 0 .0% 4 100.0%
4 100.0% 0 .0% 4 100.0%
Lama_Fermentasi
Hari ke-0
Hari ke-1
Hari ke-2
Hari ke-3
Hari ke-4
Hari ke-5
Fermentas i_Spontan
N Percent N Percent N Percent
Valid Missing Total
Cases
Tests of Nor mality
.243 4 . .932 4 .607
.239 4 . .959 4 .772
.261 4 . .881 4 .343
.251 4 . .913 4 .500
.177 4 . .989 4 .954
.214 4 . .982 4 .916
Lama_Fermentasi
Hari ke-0
Hari ke-1
Hari ke-2
Hari ke-3
Hari ke-4
Hari ke-5
Fermentas i_Spontan
Statistic df Sig. Statistic df Sig.
Kolmogorov-Smirnova
Shapiro-Wilk
Lilliefors Signif icance Correctiona.
ANOVA
Fermentas i_Spontan
10591.009 5 2118.202 228.594 .000
166.792 18 9.266
10757.801 23
Betw een Groups
Within Groups
Total
Sum of
Squares df Mean Square F Sig.
Ferm entas i_Spontan
Duncana
4 234.6875
4 235.2917
4 250.2292
4 269.2708
4 275.1875
4 291.4167
.782 1.000 1.000 1.000 1.000
Lama_Fermentasi
Hari ke-0
Hari ke-1
Hari ke-2
Hari ke-3
Hari ke-4
Hari ke-5
Sig.
N 1 2 3 4 5
Subset for alpha = .05
Means for groups in homogeneous subsets are displayed.
Uses Harmonic Mean Sample Size = 4.000.a.
Tes t of Homogene ity of Variances
Fermentas i_Spontan
.824 5 18 .549
Levene
Statistic df1 df2 Sig.
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Lampiran 7. Analisis Data Tokoferol Fermentasi Penambahan BAL
Case Process ing Summ ary
4 100.0% 0 .0% 4 100.0%
4 100.0% 0 .0% 4 100.0%
4 100.0% 0 .0% 4 100.0%
4 100.0% 0 .0% 4 100.0%
4 100.0% 0 .0% 4 100.0%
Lama_Fermentasi
Hari ke-0
Hari ke-1
Hari ke-2
Hari ke-3
Hari ke-4
Fermentas i_BAL
N Percent N Percent N Percent
Valid Missing Total
Cases
Tes ts of Normality
.214 4 . .963 4 .798
.191 4 . .973 4 .861
.239 4 . .938 4 .641
.378 4 . .804 4 .110
.238 4 . .940 4 .656
Lama_Fermentasi
Hari ke-0
Hari ke-1
Hari ke-2
Hari ke-3
Hari ke-4
Fermentas i_BAL
Statistic df Sig. Statistic df Sig.
Kolmogorov-Smirnova
Shapiro-Wilk
Lilliefors Signif icance Correctiona.
ANOVA
Fermentas i_BAL
9233.053 4 2308.263 246.111 .000
140.684 15 9.379
9373.738 19
Betw een Groups
Within Groups
Total
Sum of
Squares df Mean Square F Sig.
Test of Homogeneity of Variances
Fermentasi_BAL
5.182 4 15 .008
Levene
Statistic df1 df2 Sig.
Ferm entas i_BAL
Duncana
4 237.0208
4 252.8750
4 265.6458
4 281.3333
4 298.6667
1.000 1.000 1.000 1.000 1.000
Lama_Fermentasi
Hari ke-0
Hari ke-1
Hari ke-2
Hari ke-3
Hari ke-4
Sig.
N 1 2 3 4 5
Subset for alpha = .05
Means for groups in homogeneous subsets are displayed.
Uses Harmonic Mean Sample Size = 4.000.a.
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ANOVA
Fermentas i_Spontan
478058.8 5 95611.761 1514.264 .000
1894.222 30 63.141
479953.0 35
Betw een Groups
Within Groups
Total
Sum of
Squares df Mean Square F Sig.
Lampiran 8. Analisis Data Total Fenol Fermentasi spontan
Case Process ing Sum mary
6 100.0% 0 .0% 6 100.0%
6 100.0% 0 .0% 6 100.0%
6 100.0% 0 .0% 6 100.0%
6 100.0% 0 .0% 6 100.0%
6 100.0% 0 .0% 6 100.0%
6 100.0% 0 .0% 6 100.0%
Lama_Fermentasi
Hari ke-0
Hari ke-1
Hari ke-2
Hari ke-3
Hari ke-4
Hari ke-5
Fermentas i_Spontan
N Percent N Percent N Percent
Valid Missing Total
Cases
Tes ts of Nor mality
.271 6 .191 .855 6 .172
.307 6 .079 .858 6 .181
.142 6 .200* .991 6 .991
.170 6 .200* .957 6 .795
.274 6 .178 .914 6 .464
.208 6 .200* .947 6 .719
Lama_Fermentasi
Hari ke-0
Hari ke-1
Hari ke-2
Hari ke-3
Hari ke-4
Hari ke-5
Fermentas i_Spontan
Statistic df Sig. Statistic df Sig.
Kolmogorov-Smirnova
Shapiro-Wilk
This is a low er bound of the true signif icance.*.
Lilliefors Signif icance Correc tiona.
Test of Homogeneity of Variances
Fermentasi_Spontan
2.864 5 30 .031
Levene
Statistic df1 df2 Sig.
Ferm entas i_Spontan
Duncana
6 1038.6979
6 1053.0729
6 1118.1771
6 1145.2604
6 1275.0000
6 1356.0938
1.000 1.000 1.000 1.000 1.000 1.000
Lama_Fermentasi
Hari ke-0
Hari ke-1
Hari ke-2
Hari ke-3
Hari ke-4
Hari ke-5
Sig.
N 1 2 3 4 5 6
Subset for alpha = .05
Means for groups in homogeneous subsets are displayed.
Uses Harmonic Mean Sample Size = 6.000.a.
40
Lampiran 9. Analisis Data Total Fenol fermentasi Penambahan BAL
Case Process ing Summ ary
6 100.0% 0 .0% 6 100.0%
6 100.0% 0 .0% 6 100.0%
6 100.0% 0 .0% 6 100.0%
6 100.0% 0 .0% 6 100.0%
6 100.0% 0 .0% 6 100.0%
Lama_Fermentasi
Hari ke-0
Hari ke-1
Hari ke-2
Hari ke-3
Hari ke-4
Fermentas i_BAL
N Percent N Percent N Percent
Valid Missing Total
Cases
Tests of Normality
.260 6 .200* .828 6 .103
.145 6 .200* .995 6 .997
.154 6 .200* .967 6 .869
.215 6 .200* .948 6 .723
.200 6 .200* .941 6 .668
Lama_Fermentasi
Hari ke-0
Hari ke-1
Hari ke-2
Hari ke-3
Hari ke-4
Fermentas i_BAL
Statistic df Sig. Statistic df Sig.
Kolmogorov-Smirnova
Shapiro-Wilk
This is a low er bound of the true signif icance.*.
Lilliefors Signif icance Correctiona. Test of Homogeneity of Variances
Fermentasi_BAL
2.068 4 25 .115
Levene
Statistic df1 df2 Sig.
ANOVA
Fermentas i_BAL
387764.1 4 96941.016 2676.494 .000
905.485 25 36.219
388669.5 29
Betw een Groups
Within Groups
Total
Sum of
Squares df Mean Square F Sig.
Ferm entas i_BAL
Duncana
6 1046.0417
6 1116.3542
6 1205.5729
6 1262.0833
6 1373.3333
1.000 1.000 1.000 1.000 1.000
Lama_Fermentasi
Hari ke-0
Hari ke-1
Hari ke-2
Hari ke-3
Hari ke-4
Sig.
N 1 2 3 4 5
Subset for alpha = .05
Means for groups in homogeneous subsets are displayed.
Uses Harmonic Mean Sample Size = 6.000.a.
41
Lampiran 10. Analisis Data Tokoferol Tiap Perlakuan Fermentasi
Uji T Tokoferol Biji Kakao Pada 0 Jam Fermentasi
Uji T Tokoferol Biji Kakao Pada 24 Jam Fermentasi
Uji T Tokoferol Biji Kakao Pada 48 Jam Fermentasi
Group Statis tics
4 234.6875 3.29448 1.64724
4 237.0208 .18478 .09239
PERLAKUAN
Spontan 0 jam
BAL 0 jam
TOKOFEROL
N Mean Std. Deviation
Std. Error
Mean
Group Statis tics
4 235.2917 4.35066 2.17533
4 252.8750 1.92149 .96075
PERLAKUAN
Spontan 24 jam
BAL 24 jam
TOKOFEROL
N Mean Std. Deviation
Std. Error
Mean
Group Statis tics
4 250.2292 2.08653 1.04326
4 265.6458 4.51662 2.25831
PERLAKUAN
Spontan 48 jam
BAL 48 jam
TOKOFEROL
N Mean Std. Deviation
Std. Error
Mean
Independent Samples Test
13.303 .011 -1.414 6 .207 -2.33333 1.64983 -6.37032 1.70365
-1.414 3.019 .252 -2.33333 1.64983 -7.56530 2.89863
Equal variances
assumed
Equal variances
not assumed
TOKOFEROL
F Sig.
Levene's Test for
Equality of Variances
t df Sig. (2-tailed)
Mean
Dif ference
Std. Error
Dif ference Low er Upper
95% Conf idence
Interval of the
Dif ference
t-test for Equality of Means
Independent Samples Test
1.142 .326 -7.394 6 .000 -17.58333 2.37804 -23.40220 -11.76447
-7.394 4.127 .002 -17.58333 2.37804 -24.10620 -11.06046
Equal variances
assumed
Equal variances
not assumed
TOKOFEROL
F Sig.
Levene's Test for
Equality of Variances
t df Sig. (2-tailed)
Mean
Dif ference
Std. Error
Dif ference Low er Upper
95% Conf idence
Interval of the
Dif ference
t-test for Equality of Means
42
Independent Samples Test
.120 .741 -9.269 6 .000 -23.47917 2.53297 -29.67712 -17.28121
-9.269 5.990 .000 -23.47917 2.53297 -29.67955 -17.27879
Equal variances
assumed
Equal variances
not assumed
TOKOFEROL
F Sig.
Levene's Test for
Equality of Variances
t df Sig. (2-tailed)
Mean
Dif ference
Std. Error
Dif ference Low er Upper
95% Conf idence
Interval of the
Dif ference
t-test for Equality of Means
Uji T Tokoferol Biji Kakao Pada 72 Jam Fermentasi
Uji T Tokoferol Biji Kakao Pada 96 Jam Fermentasi
Group Statis tics
4 269.2708 1.25900 .62950
4 281.3333 3.06941 1.53471
PERLAKUAN
Spontan 72 jam
BAL 72 jam
TOKOFEROL
N Mean Std. Deviation
Std. Error
Mean
Independent Samples Test
6.017 .050 -6.197 6 .001 -15.41667 2.48764 -21.50371 -9.32962
-6.197 4.225 .003 -15.41667 2.48764 -22.18098 -8.65235
Equal variances
assumed
Equal variances
not assumed
TOKOFEROL
F Sig.
Levene's Test for
Equality of Variances
t df Sig. (2-tailed)
Mean
Dif ference
Std. Error
Dif ference Low er Upper
95% Conf idence
Interval of the
Dif ference
t-test for Equality of Means
Independent Samples Test
2.169 .191 -7.272 6 .000 -12.06250 1.65879 -16.12142 -8.00358
-7.272 3.982 .002 -12.06250 1.65879 -16.67642 -7.44858
Equal variances
assumed
Equal variances
not assumed
TOKOFEROL
F Sig.
Levene's Test for
Equality of Variances
t df Sig. (2-tailed)
Mean
Dif ference
Std. Error
Dif ference Low er Upper
95% Conf idence
Interval of the
Dif ference
t-test for Equality of Means
Group Statis tics
4 275.1875 3.50949 1.75475
4 298.6667 3.65339 1.82669
PERLAKUAN
Spontan 96 jam
BAL 96 jam
TOKOFEROL
N Mean Std. Deviation
Std. Error
Mean
43
Lampiran 11. Analisis Data Total Fenol Tiap Perlakuan Fermentasi
Uji T Total Fenol Biji Kakao Pada 0 Jam Fermentasi
Uji T Total Fenol Biji Kakao Pada 24 Jam Fermentasi
Uji T Total Fenol Biji Kakao Pada 48 Jam Fermentasi
Group Statis tics
6 1038.6979 10.59078 4.32367
6 1046.0417 2.20676 .90091
PERLAKUAN
Spontan 0 jam
BAL 0 jam
t.fenol
N Mean Std. Deviation
Std. Error
Mean
Group Statis tics
6 1053.0729 4.18991 1.71052
6 1116.3542 7.56138 3.08692
PERLAKUAN
Spontan 24 jam
BAL 24 jam
t.fenol
N Mean Std. Deviation
Std. Error
Mean
Group Statis tics
6 1118.1771 12.77726 5.21630
6 1205.5729 8.22871 3.35935
PERLAKUAN
Spontan 48 jam
BAL 48 jam
t.fenol
N Mean Std. Deviation
Std. Error
Mean
Independent Samples Test
9.135 .013 -1.663 10 .127 -7.34375 4.41653 -17.18440 2.49690
-1.663 5.433 .153 -7.34375 4.41653 -18.42979 3.74229
Equal variances
assumed
Equal variances
not assumed
t.fenol
F Sig.
Levene's Test for
Equality of Variances
t df Sig. (2-tailed)
Mean
Dif ference
Std. Error
Dif ference Low er Upper
95% Conf idence
Interval of the
Dif ference
t-test for Equality of Means
Independent Samples Test
1.063 .327 -17.931 10 .000 -63.28125 3.52916 -71.14471 -55.41779
-17.931 7.806 .000 -63.28125 3.52916 -71.45486 -55.10764
Equal variances
assumed
Equal variances
not assumed
t.fenol
F Sig.
Levene's Test for
Equality of Variances
t df Sig. (2-tailed)
Mean
Dif ference
Std. Error
Dif ference Low er Upper
95% Conf idence
Interval of the
Dif ference
t-test for Equality of Means
44
Independent Samples Test
4.033 .072 -31.801 10 .000 -98.33333 3.09219 -105.223 -91.44351
-31.801 7.053 .000 -98.33333 3.09219 -105.634 -91.03261
Equal variances
assumed
Equal variances
not assumed
t.fenol
F Sig.
Levene's Test for
Equality of Variances
t df Sig. (2-tailed)
Mean
Dif ference
Std. Error
Dif ference Low er Upper
95% Conf idence
Interval of the
Dif ference
t-test for Equality of Means
Uji T Total Fenol Biji Kakao Pada 72 Jam Fermentasi
Uji T Total Fenol Biji Kakao Pada 96 Jam Fermentasi
Group Statis tics
6 1145.2604 4.51740 1.84422
6 1262.0833 6.41856 2.62037
PERLAKUAN
Spontan 72 jam
BAL 72 jam
t.fenol
N Mean Std. Deviation
Std. Error
Mean
Group Statis tics
6 1275.0000 6.87216 2.80555
6 1373.3333 3.18484 1.30021
PERLAKUAN
Spontan 96 jam
BAL 96 jam
t.fenol
N Mean Std. Deviation
Std. Error
Mean
Independent Samples Test
1.244 .291 -14.086 10 .000 -87.39583 6.20443 -101.220 -73.57149
-14.086 8.539 .000 -87.39583 6.20443 -101.548 -73.24401
Equal variances
assumed
Equal variances
not assumed
t.fenol
F Sig.
Levene's Test for
Equality of Variances
t df Sig. (2-tailed)
Mean
Dif ference
Std. Error
Dif ference Low er Upper
95% Conf idence
Interval of the
Dif ference
t-test for Equality of Means
Independent Samples Test
1.829 .206 -36.458 10 .000 -116.82292 3.20429 -123.963 -109.683
-36.458 8.977 .000 -116.82292 3.20429 -124.074 -109.572
Equal variances
assumed
Equal variances
not assumed
t.fenol
F Sig.
Levene's Test for
Equality of Variances
t df Sig. (2-tailed)
Mean
Dif ference
Std. Error
Dif ference Low er Upper
95% Conf idence
Interval of the
Dif ference
t-test for Equality of Means
